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1. 发明授权

US08202725B2 Cell sodding method and apparatus 有权
标题翻译：细胞浸渍法和装置
公开(公告)号：US08202725B2
公开(公告)日：2012-06-19
申请号：US11314281
申请日：2005-12-22
申请人： Paul E. Kosnik , Christopher T. England , Robert G. Dennis , Stuart K. Williams
发明人： Paul E. Kosnik , Christopher T. England , Robert G. Dennis , Stuart K. Williams
IPC分类号： C12N5/00
CPC分类号： C12N5/0697 , C12M21/08 , C12M25/10 , C12M25/14 , C12M27/10 , C12M29/10 , C12N2533/30
摘要： Tissue engineering methods and biochamber apparatus are provided for making tissue grafts for implantation into a patient. The methods include applying a sustained low magnitude pressure gradient transmurally across a permeable scaffold material using a media containing cells, preferably microvascular epithelial cells, to be deposited on the scaffold for the production of tissue grafts, preferably vascular grafts, to promote accelerated adhesion and maturation of cells on the scaffold material. Biochambers for preparing tubular tissue grafts are provided which contain connectors for holding a graft substrate, proximal and distal tubing for connection to an optional perfusion system, and structure for switching between transmural flow of a cell suspension across the graft substrate and translumenal flow through the lumen of the graft.
摘要翻译：提供组织工程方法和生物处理设备用于制造植入患者体内的组织移植物。所述方法包括使用包含细胞（优选微血管上皮细胞）的培养基透过可渗透的支架材料跨度施加持续的低幅度压力梯度，以沉积在支架上用于生产组织移植物，优选血管移植物，以促进加速粘附和成熟的细胞在脚手架材料上。提供了用于制备管状组织移植物的生物体，其包含用于保持移植物基质的连接器，用于连接到任选的灌注系统的近端和远端管，以及用于在穿过移植物基质的细胞悬浮液的跨壁流和通过内腔的经血流之间切换的结构的移植物。

2. 发明申请

US20090192454A1 CATHETER FOR CELL DELIVERY 审中-公开
标题翻译：电池输送管
公开(公告)号：US20090192454A1
公开(公告)日：2009-07-30
申请号：US12418551
申请日：2009-04-03
申请人： Eugene Boland , Stuart K. Williams , Paul E. Kosnik
发明人： Eugene Boland , Stuart K. Williams , Paul E. Kosnik
IPC分类号： A61M25/10 , A61M25/00 , A61M5/178
CPC分类号： A61M25/10182 , A61M25/007 , A61M25/10184 , A61M2025/0057 , A61M2025/105 , A61M2025/1075
摘要： A cell delivery system and method for delivering cells locally to a tissue, body cavity, or joint is described. The cell delivery system comprises a catheter configured to deliver stem cells in a pressure controlled manner. The catheter may comprise an inner bladder and an outer perforated bladder. The inner bladder may be expanded through the use of a pressure conduit in order to deploy a stent. Cells, such as endothelial cells derived from adipose tissue, may be introduced between the inner and outer bladder. The inner bladder may be further expanded in order to exert pressure on the outer perforated bladder to advance the stems cells though the apertures of the outer bladder. The inner bladder may remain pressurized to hold the outer bladder against the vessel wall, thereby directing the stem cells to specific target sites. The system may be used to deliver stem cells with or without other therapeutic agents. The system may be used with or without a stent. The system may further comprise a pressure gauge that permits measurement and regulation of pressure within the catheter.
摘要翻译：描述了将细胞局部递送至组织，体腔或关节的细胞递送系统和方法。细胞递送系统包括配置成以压力控制的方式递送干细胞的导管。导管可以包括内囊和外穿孔囊。可以通过使用压力导管来扩张内囊，以便展开支架。可以在内囊和外囊之间引入细胞，例如源自脂肪组织的内皮细胞。内囊可以进一步膨胀以便在外部穿孔的膀胱上施加压力，以通过外囊的孔推进杆细胞。内囊可以保持加压以将外囊保持抵靠血管壁，从而将干细胞引导到特定的靶位点。该系统可用于递送具有或不具有其它治疗剂的干细胞。该系统可以在有或没有支架的情况下使用。该系统还可以包括允许测量和调节导管内的压力的压力计。

3. 发明申请

US20080160085A1 Cell delivery matrices 审中-公开
标题翻译：细胞递送矩阵
公开(公告)号：US20080160085A1
公开(公告)日：2008-07-03
申请号：US11901138
申请日：2007-09-13
申请人： Eugene D. Boland , Stuart K. Williams , Paul E. Kosnik
发明人： Eugene D. Boland , Stuart K. Williams , Paul E. Kosnik
IPC分类号： A61K9/00 , A61K35/12
CPC分类号： A61K9/4808 , A61K9/0019 , A61K35/36 , A61K35/44 , A61K47/42 , A61L27/16 , A61L27/18 , A61L27/225 , A61L27/24 , A61M25/104 , C12N5/0653 , C08L27/18 , C08L67/02
摘要： Cell delivery matrices and methods for facilitating local delivery of adipose derived endothelial cells to a target tissue, body cavity, or joint are described. The cell delivery matrix may be a three-dimensional matrix scaffold comprising fibrin derived from the patient's own body. The cell delivery matrix may be biocompatible and semi-permeable. The cell delivery matrix used in the methods of the invention may be comprised of any degradable, bioabsorbable or non-degradable, biocompatible polymer. Regenerative therapies comprising implanting in the subject cell delivery matrices localizing adipose derived endothelial cells are described. The cell delivery matrices maintain the adipose derived endothelial cells at the target for a therapeutically effective amount of time. The adipose derived endothelial cells can be allogenic or syngenic to the subject. The endothelial cells may be delivered alone or in combination with other therapeutic agents.
摘要翻译：描述了用于促进将脂肪来源的内皮细胞局部递送至靶组织，体腔或关节的细胞递送基质和方法。细胞递送基质可以是包含衍生自患者本身的纤维蛋白的三维基质支架。细胞递送基质可以是生物相容性的和半渗透性的。用于本发明方法的细胞递送基质可以由任何可降解的，生物可吸收的或不可降解的生物相容性聚合物组成。描述了包含植入本发明的细胞递送基质定位脂肪来源的内皮细胞的再生疗法。细胞递送基质将靶向脂肪来源的内皮细胞维持在治疗有效的时间量。脂肪来源的内皮细胞可以是同种异体的或与受试者同源的。内皮细胞可以单独递送或与其它治疗剂组合递送。

4. 发明申请

US20080199953A1 SYSTEM AND METHOD FOR FORMING A CONNECTIVE TISSUE CONSTRUCT 失效
标题翻译：用于形成连接组织结构的系统和方法
公开(公告)号：US20080199953A1
公开(公告)日：2008-08-21
申请号：US10602789
申请日：2003-06-24
申请人： Paul E. Kosnik , Robert G. Dennis , Sarah C. Calve , Ellen M. Arruda
发明人： Paul E. Kosnik , Robert G. Dennis , Sarah C. Calve , Ellen M. Arruda
IPC分类号： C12N5/06 , C12N5/08 , C12M1/00
CPC分类号： A61L27/3604 , A61K35/12 , A61L27/3804 , A61L27/386 , A61L27/3895 , C12N5/066 , C12N2500/38 , C12N2533/18 , C12N2533/52 , C12N2533/56
摘要： A system and method are provided for forming a connective tissue construct, such as a tendon construct, in vitro. A substrate is provided with at least two anchors secured thereto in spaced relationship. Fibroblast cells are provided on the substrate in the absence of a synthetic matrix, where at least some of the cells are in contact with the anchors. The cells are cultured in vitro under conditions to allow the cells to self-organize and become confluent between the anchors, where the anchors are receptive to the cells and allow the cells to attach thereto while permitting the cells to detach from the substrate to form a three-dimensional connective tissue construct.
摘要翻译：提供了用于在体外形成结缔组织构建体（例如腱结构）的系统和方法。基板具有至少两个以其间隔的关系固定到其上的锚固件。在不存在合成基质的情况下，在基质上提供成纤维细胞，其中至少一些细胞与锚接触。在体外培养细胞以允许细胞自我组织并在锚定体之间融合，其中锚固体接受细胞并允许细胞附着于细胞，同时允许细胞从基底分离以形成三维结缔组织结构。

5. 发明授权

US07422900B1 System and method for forming a connective tissue construct 失效
标题翻译：用于形成结缔组织结构的系统和方法
公开(公告)号：US07422900B1
公开(公告)日：2008-09-09
申请号：US10602789
申请日：2003-06-24
申请人： Paul E. Kosnik , Robert G. Dennis , Sarah C. Calve , Ellen M. Arruda
发明人： Paul E. Kosnik , Robert G. Dennis , Sarah C. Calve , Ellen M. Arruda
IPC分类号： C12N5/00
CPC分类号： A61L27/3604 , A61K35/12 , A61L27/3804 , A61L27/386 , A61L27/3895 , C12N5/066 , C12N2500/38 , C12N2533/18 , C12N2533/52 , C12N2533/56
摘要： A system and method are provided for forming a connective tissue construct, such as a tendon construct, in vitro. A substrate is provided with at least two anchors secured thereto in spaced relationship. Fibroblast cells are provided on the substrate in the absence of a synthetic matrix, where at least some of the cells are in contact with the anchors. The cells are cultured in vitro under conditions to allow the cells to self-organize and become confluent between the anchors, where the anchors are receptive to the cells and allow the cells to attach thereto while permitting the cells to detach from the substrate to form a three-dimensional connective tissue construct.
摘要翻译：提供了用于在体外形成结缔组织构建体（例如腱结构）的系统和方法。基板具有至少两个以其间隔的关系固定到其上的锚固件。在不存在合成基质的情况下，在基质上提供成纤维细胞，其中至少一些细胞与锚接触。在体外培养细胞以允许细胞自我组织并在锚定体之间融合，其中锚固体接受细胞并允许细胞附着于细胞，同时允许细胞从基底分离以形成三维结缔组织结构。

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