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    • 9. 发明授权
    • Functional characterization of genes
    • 基因的功能表征
    • US06300542B1
    • 2001-10-09
    • US09317378
    • 1999-05-24
    • Steven P. BriggsRobert B. Meeley
    • Steven P. BriggsRobert B. Meeley
    • A01H100
    • C12N15/8241A01H1/04C12Q1/6895
    • Insertions into a gene of known sequence can be generated by crossing two parent plants, one of which contains a transposable element, to produce F1 progeny plants in which the insertion is detected by means of a PCR. F1 progeny plants containing such an insertion are self-fertilized to produce F2 progeny which are homozygous for the insertion. The function of a gene disabled by the insertion can be ascertained from a comparison of the phenotype of the F2 progeny with a parental phenotype. Large numbers of F1 progeny can be tested simultaneously for the presence of insertions. A collection of F2 seed can be stored and used for phenotype comparison when an insertion is detected.
    • 可以通过将两个亲本植物(其中一个含有转座因子)杂交到产生F1后代植物中来产生已知序列的基因,其中通过PCR检测插入。 含有这种插入物的F1后代植物是自我受精的,以产生纯合插入的F2后代。 可以从F2后代的表型与亲本表型的比较中确定插入能够阻断的基因的功能。 可以同时测试大量的F1后代的插入物的存在。 当检测到插入时,可以存储F2种子的集合并用于表型比较。