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    • 5. 发明授权
    • Juvenile hormone or one of its agonists as a chemical ligand to control gene expression in plants by receptor mediated transactivation
    • 少年激素或其激动剂之一作为通过受体介导的反式激活控制植物中的基因表达的化学配体
    • US06362394B1
    • 2002-03-26
    • US09376107
    • 1999-08-17
    • Lyle Dean CrosslandStephen Arthur Goff
    • Lyle Dean CrosslandStephen Arthur Goff
    • C12N1582
    • C12N15/8238C07K14/70567C07K14/721C07K2319/00C12N15/8287
    • The present invention is drawn to a method of controlling gene expression in plants. Specifically, the method comprises transforming a plant with a USP receptor expression cassette which encodes a USP receptor and at least one target expression cassette which encodes a target polypeptide. Contacting said transformed plant with juvenile hormone or one of its agonists activates expression of the target polypeptide in the presence of said USP receptor polypeptide. Optionally, additional “secondary” receptor expression cassettes may be used, wherein the secondary receptor expression cassette encodes a receptor polypeptide distinct from USP. The method is useful for controlling various traits of agronomic importance, such as plant fertility. Also disclosed is a method of identifying previously unknown ligands for USP. Substances to be tested are identified by placing them in contact with plant cells transformed with a USP receptor expression cassette and a target expression cassette. The target expression cassette encodes a reporter polypeptide whose expression can be determined quantitatively or qualitatively, whereby the test substance is identified as a ligand for USP.
    • 本发明涉及控制植物中基因表达的方法。 具体地,该方法包括用编码USP受体的USP受体表达盒和至少一个编码靶多肽的目标表达盒转化植物。 将所述转化的植物与幼体激素或其一种激动剂接触在所述USP受体多肽的存在下激活靶多肽的表达。 任选地,可以使用另外的“次级”受体表达盒,其中二级受体表达盒编码与USP不同的受体多肽。 该方法可用于控制农艺学重要性的各种性状,如植物生育力。 还公开了鉴定USP以前未知的配体的方法。 通过将它们与用USP受体表达盒和目标表达盒转化的植物细胞接触来鉴定待测物质。 靶表达盒编码报告多肽,其表达可以定量或定性地测定,由此将检测物质鉴定为USP的配体。
    • 6. 发明授权
    • Fungal genes required for normal growth and development
    • 正常生长发育所需的真菌基因
    • US06291660B1
    • 2001-09-18
    • US09415522
    • 1999-10-08
    • Thomas Deane GaffneyJuergen WendlandFred DietrichPeter PhilippsenStephen Arthur Goff
    • Thomas Deane GaffneyJuergen WendlandFred DietrichPeter PhilippsenStephen Arthur Goff
    • C07H2104
    • C07K14/37
    • The present invention relates to genomic DNA sequences obtained from terminal sequencing of random genomic fragments of the filamentous fungus Ashbya gossypii, to the sequences obtained therewith and the use of the sequences for forensic identification, to characterize genes and gene organization or this ascomycete by inter-genomic comparison, to identify biosynthetic genes that can be used as selection markers, to isolate promoters and terminators for application in a homologous as well as heterologous context, to find putative centromere containing clones, chromosome mapping, chromosome identifying, general information about chromosome organization and in addition to identify ORF containing SRS sequences with no homology to S. cervisiae or any other organism which allows the identification of A. gossypii specific genes.
    • 本发明涉及从丝状真菌灰白僵菌的随机基因组片段的末端测序获得的基因组DNA序列,其获得的序列以及用于法医鉴定的序列用于表征基因和基因组织或这种子囊菌, 基因组比较,以鉴定可用作选择标记的生物合成基因,分离启动子和终止子以应用于同源和异源上下文中,以发现推定的着丝粒含有克隆,染色体定位,染色体鉴定,关于染色体组织的一般信息和 除了鉴定含有SRS序列的ORF之外,还没有与S.sarvisiae或任何其他允许鉴别羊链球菌特异性基因的生物体的同源性。