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1. 发明授权

US07776521B1 Coronavirus isolated from humans 有权
标题翻译：从人类分离的冠状病毒
公开(公告)号：US07776521B1
公开(公告)日：2010-08-17
申请号：US11748359
申请日：2007-05-14
申请人： Paul A. Rota , Larry J. Anderson , William J. Bellini , Michael D. Bowen , Cara Carthel Burns , Raymond Campagnoli , Qi Chen , James A. Comer , Byron T. Cook , Shannon L. Emery , Dean D. Erdman , Cynthia S. Goldsmith , Jeanette Guarner , Charles D. Humphrey , Joseph P. Icenogle , Thomas G. Ksiazek , Richard F. Meyer , Stephan S. Monroe , William Allan Nix , M. Steven Oberste , Christopher D. Paddock , Teresa C. T. Peret , Pierre E. Rollin , Mark A. Pallansch , Anthony Sanchez , Wun-Ju Shieh , Suxiang Tong , Sherif R. Zaki
发明人： Paul A. Rota , Larry J. Anderson , William J. Bellini , Michael D. Bowen , Cara Carthel Burns , Raymond Campagnoli , Qi Chen , James A. Comer , Byron T. Cook , Shannon L. Emery , Dean D. Erdman , Cynthia S. Goldsmith , Jeanette Guarner , Charles D. Humphrey , Joseph P. Icenogle , Thomas G. Ksiazek , Richard F. Meyer , Stephan S. Monroe , William Allan Nix , M. Steven Oberste , Christopher D. Paddock , Teresa C. T. Peret , Pierre E. Rollin , Mark A. Pallansch , Anthony Sanchez , Wun-Ju Shieh , Suxiang Tong , Sherif R. Zaki
IPC分类号： C12Q1/70 , C07H21/00 , C12N15/50
CPC分类号： C07K14/005 , A61K39/12 , A61K39/215 , C12N7/00 , C12N2770/20021 , C12N2770/20022 , C12N2770/20034
摘要： Disclosed herein is a newly isolated human coronavirus (SARS-CoV), the causative agent of severe acute respiratory syndrome (SARS). Also provided are the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of the SARS-CoV open reading frames, as well as methods of using these molecules to detect a SARS-CoV and detect infections therewith. Immune stimulatory compositions are also provided, along with methods of their use.
摘要翻译：本文公开了新分离的人冠状病毒（SARS-CoV），严重急性呼吸综合征（SARS）的致病因子。还提供了SARS-CoV基因组的核酸序列和SARS-CoV开放阅读框的氨基酸序列，以及使用这些分子检测SARS-CoV并检测其感染的方法。还提供免疫刺激组合物及其使用方法。

2. 发明授权

US07220852B1 Coronavirus isolated from humans 有权
标题翻译：从人类分离的冠状病毒
公开(公告)号：US07220852B1
公开(公告)日：2007-05-22
申请号：US10822904
申请日：2004-04-12
申请人： Paul A. Rota , Larry J. Anderson , William J. Bellini , Cara Carthel Burns , Raymond Campagnoli , Qi Chen , James A. Comer , Shannon L. Emery , Dean D. Erdman , Cynthia S. Goldsmith , Charles D. Humphrey , Joseph P. Icenogle , Thomas G. Ksiazek , Stephan S. Monroe , William Allan Nix , M. Steven Oberste , Teresa C. T. Peret , Pierre E. Rollin , Mark A. Pallansch , Anthony Sanchez , Suxiang Tong , Sherif R. Zaki
发明人： Paul A. Rota , Larry J. Anderson , William J. Bellini , Cara Carthel Burns , Raymond Campagnoli , Qi Chen , James A. Comer , Shannon L. Emery , Dean D. Erdman , Cynthia S. Goldsmith , Charles D. Humphrey , Joseph P. Icenogle , Thomas G. Ksiazek , Stephan S. Monroe , William Allan Nix , M. Steven Oberste , Teresa C. T. Peret , Pierre E. Rollin , Mark A. Pallansch , Anthony Sanchez , Suxiang Tong , Sherif R. Zaki
IPC分类号： C12N15/50 , C12N7/00
CPC分类号： C07K14/005 , A61K39/12 , A61K39/215 , C12N7/00 , C12N2770/20021 , C12N2770/20022 , C12N2770/20034
摘要： Disclosed herein is a newly isolated human coronavirus (SARS-CoV), the causative agent of severe acute respiratory syndrome (SARS). Also provided are the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of the SARS-CoV open reading frames, as well as methods of using these molecules to detect a SARS-CoV and detect infections therewith. Immune stimulatory compositions are also provided, along with methods of their use.
摘要翻译：本文公开了新分离的人冠状病毒（SARS-CoV），严重急性呼吸综合征（SARS）的致病因子。还提供了SARS-CoV基因组的核酸序列和SARS-CoV开放阅读框的氨基酸序列，以及使用这些分子检测SARS-CoV并检测其感染的方法。还提供免疫刺激组合物及其使用方法。

3. 发明授权

US5316910A Bioassay for influenza A and B nucleoprotein 失效
标题翻译： A型和B型甲基蛋白的生物测定
公开(公告)号：US5316910A
公开(公告)日：1994-05-31
申请号：US15739
申请日：1993-02-10
申请人： Paul A. Rota , Renee A. Black
发明人： Paul A. Rota , Renee A. Black
IPC分类号： A61K38/00 , A61K39/00 , C07K14/11 , C07K16/10 , C12N15/866 , C12Q1/00
CPC分类号： C12N15/86 , C07K14/005 , C07K16/1018 , A61K38/00 , A61K39/00 , C12N2710/14143 , C12N2740/16134 , C12N2740/16234 , C12N2760/16122
摘要： The present invention relates to nucleoprotein (NP) genes of the influenza A and B viruses which were constructed from virion RNA and subsequently expressed in Spodoptera frugiperda (S19) cells using the baculovirus vector, Autographa californica nuclear polyhedrosis virus (AcNPV). Western blot analysis of lysates prepared from S19 cells infected with the recombinant viruses confirmed that the baculovirus-expressed NP antigens were reactive with monoclonal antibodies specific for either type A or B NP and with anti-NP antibodies in human serum samples. Electrophoretic analysis indicated that the expressed influenza NP antigens comigrated with NP purified from influenza A or B virions and that the recombinant NP antigens represented greater than 10% of total protein in infected cells. Dilutions of clarified S19 cell lysates were used as antigens in a standard enzyme immunoassay format to detect serum antibody specific for influenza A or B viruses. The results from assays using the baculovirus-expressed NP antigens showed good correlation with the results obtained using bacterially-expressed NP antigen as well as complement fixation. Therefore, baculovirus-expressed NP antigens can be used in reproducible and routine assays for the serodiagnosis of influenza virus infections as an alternative to the more burdensome complement fixation or hemagglutination tests.
摘要翻译：本发明涉及由病毒粒子RNA构建并随后使用杆状病毒载体（Autographa californica nuclear polyhedrosis virus，AcNPV）在草地贪夜蛾（S-19）细胞中表达的甲型和乙型流感病毒的核蛋白（NP）基因。用重组病毒感染的S19细胞制备的裂解物的Western印迹分析证实，杆状病毒表达的NP抗原与人血清样品中针对A型或B型NP特异性抗体和抗NP抗体的单克隆抗体反应。电泳分析表明，表达的流感NP抗原与从A型或B型病毒粒子中纯化的NP一起游离，并且重组NP抗原在感染细胞中占总蛋白的10％以上。将澄清的S19细胞裂解物的稀释液用作标准酶免疫测定法的抗原，以检测甲型或乙型流感病毒特异性的血清抗体。使用杆状病毒表达的NP抗原的测定结果与使用细菌表达的NP抗原以及补体固定获得的结果具有良好的相关性。因此，杆状病毒表达的NP抗原可用于可重复和常规检测流感病毒感染的血清学诊断，作为更繁重的补体固定或血细胞凝集试验的替代方法。

4. 发明授权

US5374717A Sequences of the hemagglutinins of recent strains of influenza type B virus 失效
标题翻译：近来B型流感病毒血凝素的序列
公开(公告)号：US5374717A
公开(公告)日：1994-12-20
申请号：US954840
申请日：1992-09-30
申请人： Paul A. Rota , Mark L. Hemphill
发明人： Paul A. Rota , Mark L. Hemphill
IPC分类号： A61K39/00 , C07K14/11 , C07H21/04
CPC分类号： C07K14/005 , A61K39/00 , C12N2760/16222
摘要： The invention provides sequence analyses for recent strains of Influenza Type B Virus.
摘要翻译：本发明提供了近来B型流感病毒株的序列分析。

5. 发明授权

US5290686A Expression of influenza a M2 protein in baculovirus 失效
标题翻译：流感病毒M2蛋白在杆状病毒中的表达
公开(公告)号：US5290686A
公开(公告)日：1994-03-01
申请号：US738032
申请日：1991-07-31
申请人： Alan P. Kendal , Renee Black , Paul A. Rota
发明人： Alan P. Kendal , Renee Black , Paul A. Rota
IPC分类号： C07K14/195 , C07K14/005 , C07K14/11 , C07K14/705 , C12N5/10 , C12N7/00 , C12N15/09 , C12P21/00 , C12R1/91 , G01N33/569 , C12P21/02
CPC分类号： C07K14/005 , C12N2710/14143 , C12N2760/16122 , C12N2760/16222
摘要： The present invention relates to baculovirus-expressed influenza antigens, in particular, to the influenza A membrane protein, M2, expressed from Autographa Californica nuclear polyhedrosis virus (AcNPV). The present invention further relates to a method to increase the yield of baculovirus-expressed M2 proteins in host cells by culturing the recombinant baculovirus infected host cells with an amantadine-like drug. Other aspect of the present invention relate to the use of baculovirus-expressed M2 proteins in reproducible and routine assays for the seradiagnosis of influenza A virus infections as an alternative to the more burdensome complement fixation and hemagglutination tests.
摘要翻译：本发明涉及杆状病毒表达的流感抗原，特别涉及由Autographa Californica核多角体病毒（AcNPV）表达的甲型流感病毒蛋白M2。本发明还涉及通过用金刚烷胺类药物培养重组杆状病毒感染的宿主细胞来增加宿主细胞中杆状病毒表达的M2蛋白的产量的方法。本发明的另一方面涉及杆状病毒表达的M2蛋白在可重复和常规测定中用于甲型流感病毒感染的血清学诊断，作为更繁重的补体固定和血细胞凝集试验的替代方案。

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