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1. 发明授权

US5466574A Apparatus and methods for magnetic separation featuring external magnetic means 失效
标题翻译：用于磁分离的装置和方法，具有外部磁性装置
公开(公告)号：US5466574A
公开(公告)日：1995-11-14
申请号：US6071
申请日：1993-01-15
申请人： Paul A. Liberti , Yuzhou Wang , Weixin Tang , Brian P. Feeley , Dhanesh I. Gohel
发明人： Paul A. Liberti , Yuzhou Wang , Weixin Tang , Brian P. Feeley , Dhanesh I. Gohel
IPC分类号： B03C1/00 , A23L3/32 , B01D35/06 , B01D43/00 , B03C1/025 , B03C1/035 , G01N33/543 , G01N33/553 , G01N33/577 , G01N35/00 , C12Q1/70
CPC分类号： B03C1/286 , A23L3/32 , B03C1/035 , G01N33/54326 , B03C2201/18 , B03C2201/26 , G01N35/0098 , Y10S435/971 , Y10S436/807 , Y10S436/824 , Y10S436/827 , Y10S436/828
摘要： A magnetic separator for isolating magnetically-labeled substances of interest, such as immunological agents, from a non-magnetic test medium using a method of high gradient magnetic separation. The target substance is contacted with microscopic magnetic particles having a receptor for binding with the target substance. The test medium containing the magnetic particles is held in a non-magnetic container and placed into a gap within an arrangement of magnets for causing the magnetic particles to adhere to selected locations upon the interior wall of the container. The quantity of magnetic particles may be controlled to cause the magnetic particles collected upon the interior wall to form a monolayer. The magnets are arranged upon a yoke which may provide linear, surrounding multipolar, or partially surrounding multipolar configurations of magnetic pole faces about the gap. The magnetic pole faces may be of differing cross-sectional areas to enhance the magnetic field gradient in regions adjacent to the smaller pole faces. The method and apparatus may be used for such investigations as enzyme-labeled competitive immunoassay and "sandwich" immunoassays.
摘要翻译：一种使用高梯度磁选法从非磁性测试介质中分离磁性标记物质的磁分离器，例如免疫学试剂。目标物质与具有与靶物质结合的受体的微观磁性粒子接触。将包含磁性颗粒的测试介质保持在非磁性容器中并放置在磁体布置内的间隙中，以使磁性颗粒粘附到容器内壁上的选定位置。可以控制磁性颗粒的量以使收集在内壁上的磁性颗粒形成单层。磁体布置在磁轭上，该磁轭可围绕间隙提供线性的，围绕多极的或部分围绕的多极配置的磁极面。磁极面可以具有不同的横截面积，以增强与较小极面相邻的区域中的磁场梯度。该方法和装置可用于诸如酶标记的竞争性免疫测定和“三明治”免疫测定的这种研究。

2. 发明授权

US5795470A Magnetic separation apparatus 失效
标题翻译：磁选机
公开(公告)号：US5795470A
公开(公告)日：1998-08-18
申请号：US482636
申请日：1995-06-07
申请人： Yuzhou Wang , Weixin Tang , Paul A. Liberti
发明人： Yuzhou Wang , Weixin Tang , Paul A. Liberti
IPC分类号： A23L3/32 , B03C1/035 , G01N35/00 , B01D35/06
CPC分类号： A23L3/32 , B03C1/035 , G01N35/0098
摘要： A magnetic separation apparatus is disclosed herein. The apparatus includes a flow-through vessel defining a plenum and an inlet port for introducing fluid into the plenum. A hydrodynamic damping structure is provided between the inlet port and plenum for reducing turbulence in the fluid. A mounting device is provided with a slot therein for supporting and receiving the vessel. The mounting device also supports a first array of magnets along one side of the slot and arranged adjacent one another to face the slot with alternating polarity, and a second array of magnets along the opposite side of the slot from the first array arranged adjacent one another to face the slot with alternating polarity and confronting and magnetically opposing the magnets in the first array. The first and second arrays of magnets produce a magnetic field within the plenum having a field strength that is maximum at a location at the interior surface of a wall of the vessel and that is minimum at a geometrical center of the plenum so that magnetic particles in the fluid are attracted to and immobilized on the inner surface of the vessel.
摘要翻译：本文公开了磁分离装置。该装置包括限定通气室的流通容器和用于将流体引入通风室的入口端口。在入口和集气室之间设有流体动力学阻尼结构，以减少流体中的湍流。安装装置在其中设置有用于支撑和接收容器的狭槽。安装装置还支撑沿着槽的一侧的第一磁体阵列并彼此相邻地布置以与交替极性相对的槽，并且沿与狭槽相邻的第一阵列的相对侧的第二磁体阵列相邻布置面对具有交替极性的槽，并且与第一阵列中的磁体相对并且磁性地相对。第一和第二磁体阵列在集气室内产生磁场，其中磁场强度在容器的壁的内表面处的位置处是最大的，并且在集气室的几何中心处最小，使得磁性颗粒在流体被吸引并固定在容器的内表面上。

3. 发明授权

US5541072A Method for magnetic separation featuring magnetic particles in a multi-phase system 失效
标题翻译：在多相系统中磁性粒子的磁分离方法
公开(公告)号：US5541072A
公开(公告)日：1996-07-30
申请号：US228818
申请日：1994-04-18
申请人： Yuzhou Wang , Weixin Tang , William J. Cronin , Paul A. Liberti
发明人： Yuzhou Wang , Weixin Tang , William J. Cronin , Paul A. Liberti
IPC分类号： B03C1/01 , B03C1/28 , B03C1/32 , C12M3/00 , G01N33/543 , G01N35/00 , G01N35/02 , H01F1/44 , G01N33/567 , G01N33/569
CPC分类号： G01N35/026 , B03C1/01 , B03C1/288 , B03C1/32 , C12M47/04 , G01N33/54326 , H01F1/44 , G01N35/0098 , Y10S436/807 , Y10S436/824 , Y10T436/25375
摘要： Methods and devices are provided for separation of magnetic particles and/or magnetic-associated substances from non-magnetic associated substances and media. The methods are specifically applicable to biological separation, and utilize a phase phenomenon arising from mutual interactions between suspended magnetic particles and interactions with the suspension medium. The phenomenon is exploited to produce and maintain a distinct, structured phase of magnetic particles, or ferrophase, within a multi-phase liquid system. A ferrophase is established within a separation chamber prior to introducing therein a test sample containing the target substances to be separated. The formation of a ferrophase is used to transport target substances from regions of relatively low magnetic field gradient to regions of relatively high magnetic field gradient within a high-gradient magnetic separation apparatus. Such transport of the ferrophase is accomplished at greater speed than the transport of individual magnetic particles within a single liquid phase, thereby enabling a more effective separation of magnetic from non-magnetic components.
摘要翻译：提供了用于将磁性颗粒和/或磁性相关物质与非磁性相关物质和介质分离的方法和装置。该方法特别适用于生物分离，并利用由悬浮磁性颗粒与悬浮介质的相互作用引起的相位现象。这种现象被用于在多相液体系统内产生和维持磁性颗粒或热解酶的独特结构化阶段。在引入含有待分离的目标物质的测试样品之前，在分离室内建立热解酶。铁高杆菌的形成用于将靶物质从相对低的磁场梯度的区域输送到高梯度磁力分离装置内的相对高的磁场梯度的区域。富马酸酶的这种运输以比在单个液相内单个磁性颗粒的输送更快的速度来实现，从而能够更有效地将磁性与非磁性组分分离。

4. 发明申请

US20210363508A1 CELL DATA RECORDERS AND USES THEREOF 有权
公开(公告)号：US20210363508A1
公开(公告)日：2021-11-25
申请号：US16970115
申请日：2019-08-22
申请人： David R, Liu , Weixin Tang , The Broad Institute, Inc.
发明人： David R. Liu , Weixin Tang
IPC分类号： C12N9/22 , C12N15/11 , C12Q1/02
摘要： Described herein are compositions, vectors, cells, methods, and kits that provide cell data recorder systems for recording cell states. The cell data recorder systems allow for the recording of both the presence and duration of one or more stimuli in a programmable, reproducible, and multiplexable manner. These cell data recorder systems employ a nucleic acid programmable DNA binding protein, such as a Cas9 nuclease, or a fusion protein comprising a nucleic acid programmable DNA binding domain and a nucleic acid editing domain to introduce recordable changes in the genome of a cell or in a plasmid within the cell.

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