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1. 发明申请

US20170226460A1 CELL CULTURE METHOD IN A BIOREACTOR SYSTEM USING MICRO-CARRIERS 审中-公开
公开(公告)号：US20170226460A1
公开(公告)日：2017-08-10
申请号：US15496753
申请日：2017-04-25
申请人： PBS Biotech, Inc.
发明人： Chanyong Brian LEE , Yasunori HASHIMURA
IPC分类号： C12M1/12 , C12M1/00 , C12M1/04 , C12N1/00 , C12M1/26
CPC分类号： C12M25/16 , C12M27/04 , C12M27/06 , C12M29/04 , C12M29/06 , C12M33/14 , C12M37/00 , C12M41/00 , C12N1/00 , Y10T137/0318
摘要： A system and method for compartmentalizing micro-carriers in a bioreactor includes a container configured to store micro-carriers and a vessel configured to contain a fluid so as to prevent the micro-carriers from entering the vessel during sterilization, shipping, storage, or other pre-use handling of the system. One or more addition lines connect the container and the vessel such that the vessel is in fluid communication with the vessel. The one or more addition lines are contactable by at least one blocking element configured to reversibly block fluid flow between the container and the vessel. The container, addition lines, and vessel are configured to allow the micro-carriers to be injected into the vessel at any point during a cell culture run. The vessel may also include a rotatable wheel, a harvest port configured to allow for the removal of the micro-carriers from the vessel, and a media removal port comprising a retention screen for removing spent medium.

2. 发明申请

US20140196791A1 METHOD AND APPARATUS FOR THE USE OF MICRO-CARRIERS IN A DISPOSABLE BIOREACTOR SYSTEM 有权
标题翻译：使用微载体在可处理的生物反应器系统中的方法和装置
公开(公告)号：US20140196791A1
公开(公告)日：2014-07-17
申请号：US14153679
申请日：2014-01-13
申请人： PBS BIOTECH, Inc.
发明人： Chanyong Brian LEE , Yasunori HASHIMURA
IPC分类号： C12M1/12
CPC分类号： C12M25/16 , C12M27/04 , C12M27/06 , C12M29/04 , C12M29/06 , C12M33/14 , C12M37/00 , C12M41/00 , C12N1/00 , Y10T137/0318
摘要： A system and method for compartmentalizing micro-carriers in a bioreactor includes a container configured to store micro-carriers and a vessel configured to contain a fluid so as to prevent the micro-carriers from entering the vessel during sterilization, shipping, storage, or other pre-use handling of the system. One or more addition lines connect the container and the vessel such that the vessel is in fluid communication with the vessel. The one or more addition lines are contactable by at least one blocking element configured to reversibly block fluid flow between the container and the vessel. The container, addition lines, and vessel are configured to allow the micro-carriers to be injected into the vessel at any point during a cell culture run. The vessel may also include a rotatable wheel, a harvest port configured to allow for the removal of the micro-carriers from the vessel, and a media removal port comprising a retention screen for removing spent medium.
摘要翻译：用于在生物反应器中分隔微载体的系统和方法包括：构造成存储微载体的容器和构造成容纳流体的容器，以便在灭菌，运输，储存或其它过程中防止微载体进入容器系统的预先使用处理。一个或多个添加管线连接容器和容器，使得容器与容器流体连通。一个或多个添加管线可被至少一个阻挡元件接触，该阻挡元件被构造成可逆地阻止容器和容器之间的流体流动。容器，添加管线和容器构造成允许微细载体在细胞培养运行期间的任何点注入血管。容器还可以包括可旋转轮，收集口构造成允许从容器移除微载体，以及介质移除端口，其包括用于移除废介质的保留屏。

3. 发明公开

US20240141275A1 SYSTEMS AND METHODS FOR EXPANSION AND DIFFERENTIATION OF THERAPEUTIC CELLS IN BIOREACTORS 审中-公开
公开(公告)号：US20240141275A1
公开(公告)日：2024-05-02
申请号：US16517902
申请日：2019-07-22
申请人： PBS Biotech, Inc.
发明人： Chanyong Brian LEE
IPC分类号： C12M1/00
CPC分类号： C12M29/18 , C12M29/14 , C12M29/26
摘要： Systems and methods for complete medium exchange using two bioreactors and at least one external separation and retention device designed for therapeutic cells grown as aggregates, on the surface of microcarriers, or as single cells, for scalable expansion and/or directed differentiation.

4. 发明申请

US20200002668A1 CONTROL OF CELL GROWTH AND AGGREGATE SIZE IN BIOREACTORS 审中-公开
公开(公告)号：US20200002668A1
公开(公告)日：2020-01-02
申请号：US16460021
申请日：2019-07-02
申请人： PBS Biotech, Inc.
发明人： Chanyong Brian LEE , Sunghoon JUNG , Yasunori Hashimura
IPC分类号： C12N5/00
摘要： Methods of repeated aggregate dissociation and reformation of pluripotent stem cells (PSCs) within the same bioreactor until a desired final cell number is achieved. A preferred step-wise process for controlled growth of PSCs and aggregate size using periodic dissociation with a dissociation medium which contains either proteolytic enzymes or chemical reagents, mechanical agitation, or a combination of these methods.

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