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1. 发明授权

US5508171A Assay method with enzyme electrode system 失效
标题翻译：酶电极系统的测定方法
公开(公告)号：US5508171A
公开(公告)日：1996-04-16
申请号：US198407
申请日：1994-02-22
申请人： P. Douglas Walling , Joseph E. Bateson , Lance S. Kuhn , M. Luann Ochs , Kent M. Kost , Martin T. Gerber , Klaus H. Pollmann , Chi-Neng A. Han
发明人： P. Douglas Walling , Joseph E. Bateson , Lance S. Kuhn , M. Luann Ochs , Kent M. Kost , Martin T. Gerber , Klaus H. Pollmann , Chi-Neng A. Han
IPC分类号： C12Q1/00 , C12Q1/26 , C12Q1/54
CPC分类号： C12Q1/004 , Y10S435/817
摘要： A new electrochemical biosensor and method of its use. The electrochemical biosensor has a pair of electrodes consisting of a working electrode and a counter electrode made of the same electrically conducting materials. The counter electrode may be the same size or smaller than the working electrode. The biosensor includes a sample receiving portion wherein a portion of the electrode surfaces is covered by a reagent that includes a redox mediator and an enzyme. A fluid that contains an analyte is added to the sample receiving portion. A reaction involving the analyte, enzyme and redox mediator occurs. After this reaction is complete, an electrical potential difference is applied between the electrodes. Diffusion limited current is measured and correlated to the concentration of analyte in the fluid.
摘要翻译：一种新的电化学生物传感器及其使用方法。电化学生物传感器具有由工作电极和由相同导电材料制成的对电极组成的一对电极。对电极的尺寸可以与工作电极相同。生物传感器包括样品接收部分，其中一部分电极表面被包含氧化还原介体和酶的试剂覆盖。含有分析物的流体被添加到样品接收部分。发生涉及分析物，酶和氧化还原介体的反应。在该反应完成之后，在电极之间施加电位差。测量扩散限制电流并与流体中分析物的浓度相关。

2. 发明授权

US5762770A Electrochemical biosensor test strip 失效
标题翻译：电化学生物传感器试片
公开(公告)号：US5762770A
公开(公告)日：1998-06-09
申请号：US496939
申请日：1995-06-30
申请人： G. John Pritchard , Joseph E. Bateson , Brian S. Hill , Brian A. Heald , Scott E. Hubbard
发明人： G. John Pritchard , Joseph E. Bateson , Brian S. Hill , Brian A. Heald , Scott E. Hubbard
IPC分类号： G01N27/327 , C12Q1/00 , C12Q1/54
CPC分类号： C12Q1/00 , C12Q1/006
摘要： An electrochemical biosensor test strip that has a minimum volume blood sample requirement of about 9 microliters. The test strip has working and counter electrodes that are substantially the same size and made of the same electrically conducting material placed on a first insulating substrate. Overlaying the electrodes is a second insulating substrate, which includes a cutout portion that forms a reagent well. The cutout portion exposes a smaller area of the counter electrode than the working electrode. A reagent for analysis of an analyte substantially covers the exposed areas of working and counter electrodes in the reagent well. Overlaying the reagent well and affixed to the second insulating substrate is a spreading mesh that is impregnated with a surfactant. The small cutout portion of 4 millimeters by 4.2 millimeters, small mesh of 6 millimeters by 5.8 millimeters, and small amount of reagent, 4 microliters before drying, allow the test strip to analyze a whole blood sample of about 9 microliters.
摘要翻译：电化学生物传感器测试条具有约9微升的最小体积血液样本要求。测试条具有基本上相同尺寸并由放置在第一绝缘基板上的相同导电材料制成的工作对电极。覆盖电极是第二绝缘基板，其包括形成试剂的切口部分。切除部分暴露对电极的面积比工作电极的面积小。用于分析分析物的试剂基本上覆盖试剂阱中的工作和对电极的暴露区域。将试剂叠加并固定到第二绝缘基底上的是用表面活性剂浸渍的铺展网。 4毫米×4.2毫米的小切口部分，6毫米×5.8毫米的小网孔和少量试剂，干燥前4微升，允许测试条分析约9微升的全血样品。

3. 发明授权

US5627075A Stable diagnostic reagent 失效
标题翻译：稳定的诊断试剂
公开(公告)号：US5627075A
公开(公告)日：1997-05-06
申请号：US619118
申请日：1996-03-20
申请人： Joseph E. Bateson
发明人： Joseph E. Bateson
IPC分类号： C12N9/04 , C12N9/96 , C12Q1/00 , C12Q1/26 , C12Q1/54 , G01N31/00
CPC分类号： C12N9/0006 , C12N9/96 , C12Q1/006 , C12Q1/54 , Y10T436/10 , Y10T436/104998 , Y10T436/144444 , Y10T436/2525
摘要： The inclusion of succinic acid or a salt thereof to stabilize compositions that include glucose oxidase and/or ferricyanide. Typically, the succinic acid or succinic acid salt is included in a diagnostic reagent utilized in test strips or test solutions useful for the analysis of glucose from a fluid sample.
摘要翻译：包含琥珀酸或其盐以稳定包括葡萄糖氧化酶和/或铁氰化物的组合物。通常，琥珀酸或琥珀酸盐被包括在用于从流体样品分析葡萄糖的测试条或测试溶液中使用的诊断试剂中。

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