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1. 发明授权

US09005526B2 Narrow bore porous layer open tube capillary column and uses thereof 有权
标题翻译：窄孔多孔层开管毛细管柱及其用途
公开(公告)号：US09005526B2
公开(公告)日：2015-04-14
申请号：US14048378
申请日：2013-10-08
申请人： Northeastern University
发明人： Barry L. Karger , Jian Zhang
IPC分类号： G01N30/02 , G01N30/60 , B01J20/28 , B01J20/285 , B01J20/289 , B01J20/32 , G01N30/72 , G01N30/52
CPC分类号： G01N30/60 , B01J20/28085 , B01J20/285 , B01J20/289 , B01J20/327 , B01J20/3282 , B01J2220/54 , B01J2220/84 , B01J2220/86 , G01N30/6073 , G01N30/7266 , G01N2030/528 , Y10T436/11 , Y10T436/117497
摘要： A polymer-based PLOT capillary column prepared by in situ copolymerization of a functional monomer and a crosslinking monomer, which enhances the strength of the polymer matrix, is disclosed. Also disclosed is a system comprising the polymer-based PLOT column coupled to a mass flow or concentration sensitive detector, for carrying out a chemical analysis method on samples separated by liquid chromatography using the column, and a process for using the system. Columns of the invention can be prepared in a robust fashion with a very narrow i.d., e.g., 5-15 μm. Thus, they are suitable for commercial use in ultratrace LC/MS proteomic analysis. Columns according to the invention are characterized by high resolving power and high column-to-column reproducibility. When these columns are coupled on-line with, e.g., ESI-MS detection, the resulting systems are capable of detecting the component parts of complex proteomic samples down to the low attomole to sub-attomole level.
摘要翻译：公开了通过功能单体和交联单体的原位共聚制备的聚合物基PLOT毛细管柱，其增强聚合物基质的强度。还公开了一种系统，其包含与质量流量或浓度敏感检测器相连的基于聚合物的PLOT柱，用于对通过使用该柱的液相色谱分离的样品进行化学分析方法，以及使用该系统的方法。可以以非常窄的i.d.例如5-15μm的稳健方式制备本发明的色谱柱。因此，它们适用于超临界LC / MS蛋白质组学分析中的商业用途。根据本发明的色谱柱的特征在于高分辨能力和高柱色谱重现性。当这些柱在线与例如ESI-MS检测偶联时，所得到的系统能够检测复合蛋白质组样品的组分部分，直至低阿托莫尔至亚阿托莫尔水平。

2. 发明申请

US20140033804A1 NARROW BORE POROUS LAYER OPEN TUBE CAPILLARY COLUMN AND USES THEREOF 有权
标题翻译： NARROW BORE POROUS LAYER OPEN TUBE CAPILLARY COLUMN及其用途
公开(公告)号：US20140033804A1
公开(公告)日：2014-02-06
申请号：US14048378
申请日：2013-10-08
申请人： Northeastern University
发明人： Barry L. Karger , Jian Zhang
IPC分类号： G01N30/60
CPC分类号： G01N30/60 , B01J20/28085 , B01J20/285 , B01J20/289 , B01J20/327 , B01J20/3282 , B01J2220/54 , B01J2220/84 , B01J2220/86 , G01N30/6073 , G01N30/7266 , G01N2030/528 , Y10T436/11 , Y10T436/117497
摘要： A polymer-based PLOT capillary column prepared by in situ copolymerization of a functional monomer and a crosslinking monomer, which enhances the strength of the polymer matrix, is disclosed. Also disclosed is a system comprising the polymer-based PLOT column coupled to a mass flow or concentration sensitive detector, for carrying out a chemical analysis method on samples separated by liquid chromatography using the column, and a process for using the system. Columns of the invention can be prepared in a robust fashion with a very narrow i.d., e.g., 5-15 μm. Thus, they are suitable for commercial use in ultratrace LC/MS proteomic analysis. Columns according to the invention are characterized by high resolving power and high column-to-column reproducibility. When these columns are coupled on-line with, e.g., ESI-MS detection, the resulting systems are capable of detecting the component parts of complex proteomic samples down to the low attomole to sub-attomole level.
摘要翻译：公开了通过功能单体和交联单体的原位共聚制备的聚合物基PLOT毛细管柱，其增强聚合物基质的强度。还公开了一种系统，其包含与质量流量或浓度敏感检测器相连的基于聚合物的PLOT柱，用于对通过使用该柱的液相色谱分离的样品进行化学分析方法，以及使用该系统的方法。可以以非常窄的i.d.例如5-15mum的稳健方式制备本发明的色谱柱。因此，它们适用于超临界LC / MS蛋白质组学分析中的商业用途。根据本发明的色谱柱的特征在于高分辨能力和高柱色谱重现性。当这些柱在线与例如ESI-MS检测偶联时，所得到的系统能够检测复合蛋白质组样品的组分部分，直至低阿托莫尔至亚阿托莫尔水平。

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