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    • 2. 发明授权
    • Self contained biological indicator
    • 自包含生物指标
    • US5736355A
    • 1998-04-07
    • US645259
    • 1996-05-13
    • Denis G. DykePaul S. MalcheskyRaymond C. KralovicDonna M. RichardsonJoseph J. Switka
    • Denis G. DykePaul S. MalcheskyRaymond C. KralovicDonna M. RichardsonJoseph J. Switka
    • C12M1/28C12Q1/18C12Q1/22C12M1/34
    • C12Q1/18C12M23/32C12M23/38C12M25/02C12M25/06C12M37/04C12Q1/22
    • A self contained biological indicator (A) determines an effectiveness of a microbial decontamination process. A dart (68) has a seal (80) engaged by rim (58) in detents (50C) in fins (36A-36F) of a cap (C). The dart has a cutting edge (76) aligned just above a foil seal (86) sealing a growth medium (F) in a medium housing (D). The dart has a chamber (72) in which challenge spores or other microorganisms on a paper disk (74) are housed. A microporous membrane (G) traps the challenge spores in the chamber while allowing a microbial decontamination fluid to flow through to contact the spores. The housing is lodged by a flange (56) in detents (50A) in fins (36A-36F). After the microbial decontamination process is complete, the cap is forced toward medium housing causing the rim and the flange to dislodge from their respective detents and lodge in detents (50B) resulting in the puncturing of the foil seal, the sealing of the dart within the medium housing and the immersion of the dart and the challenge spores into the growth medium.
    • 自给自足的生物指示剂(A)确定微生物净化过程的有效性。 飞镖(68)具有在盖(C)的翅片(36A-36F)中的掣子(50C)中与轮辋(58)接合的密封件(80)。 飞镖具有在密封培养基壳体(D)中的生长介质(F)的箔密封件(86)正上方对准的切割边缘(76)。 飞镖具有容纳在纸盘(74)上的挑战孢子或其他微生物的室(72)。 微孔膜(G)捕获腔室中的挑战孢子,同时允许微生物去污染液体流过以接触孢子。 壳体通过翼片(36A-36F)中的棘爪(50A)中的凸缘(56)放置。 微生物净化过程完成后,盖被迫朝向中等壳体,导致轮缘和凸缘从它们各自的棘爪上移开,并且卡在掣子(50B)中,导致穿刺箔片密封件,将飞镖密封在 中等住房和镖的浸入和挑战孢子进入生长培养基。