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    • 4. 发明申请
    • Tissue scaffold having aligned fibrils, apparatus and method for producing the same, and artificial tissue and methods of use thereof
    • 具有对齐原纤维的组织支架,其制造方法和人造组织及其使用方法
    • US20050009178A1
    • 2005-01-13
    • US10861664
    • 2004-06-04
    • Michael YostC. GoreLouis TerracioRichard GoodwinEdie Goldsmith
    • Michael YostC. GoreLouis TerracioRichard GoodwinEdie Goldsmith
    • A61F2/02A61F2/04A61F2/06A61K38/17C12M3/00C12N20060101C12N5/08C12N11/02
    • B29C47/24B29C47/0023C12M21/08C12M25/14
    • A tubular tissue scaffold is described which comprises a tube having a wall, wherein the wall includes biopolymer fibrils that are aligned in a helical pattern around the longitudinal axis of the tube where the pitch of the helical pattern changes with the radial position in the tube wall. The scaffold is capable of directing the morphological pattern of attached and growing cells to form a helical pattern around the tube walls. Additionally, an apparatus for producing such a tubular tissue scaffold is disclosed, the apparatus comprising a biopolymer gel dispersion feed pump that is operably connected to a tube-forming device having an exit port, where the tube-forming device is capable of producing a tube from the gel dispersion while providing an angular shear force across the wall of the tube, and a liquid bath located to receive the tubular tissue scaffold from the tube-forming device. A method for producing the tubular tissue scaffolds is also disclosed. Also, artificial tissue comprising living cells attached to a tubular tissue scaffold as described herein is disclosed. Methods for using the artificial tissue are also disclosed.
    • 描述了一种管状组织支架,其包括具有壁的管,其中所述壁包括围绕所述管的纵向轴线以螺旋形图案对准的生物聚合物原纤维,其中螺旋形图案的间距随管壁中的径向位置而变化 。 支架能够引导附着和生长的细胞的形态模式以在管壁周围形成螺旋图案。 此外,公开了一种用于生产这种管状组织支架的装置,该装置包括生物聚合物凝胶分散体供给泵,其可操作地连接到具有出口的管形成装置,其中管形成装置能够产生管 从所述凝胶分散体提供穿过所述管壁的角度剪切力,以及液体浴,其位于从所述管形成装置接收所述管状组织支架。 还公开了一种用于生产管状组织支架的方法。 此外,公开了包含附着在本文所述的管状组织支架上的活细胞的人造组织。 还公开了使用人造组织的方法。