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1. 发明授权

US06340741B1 Potent genetic switch allowing regulated gene expression in eukaryotic cells 失效
标题翻译：有力的基因转换允许在真核细胞中调节基因表达
公开(公告)号：US06340741B1
公开(公告)日：2002-01-22
申请号：US09370683
申请日：1999-08-09
申请人： Nicolas Mermod , Markus O. Imhof , Philippe Chatellard
发明人： Nicolas Mermod , Markus O. Imhof , Philippe Chatellard
IPC分类号： C07K1400
CPC分类号： C12N15/635
摘要： The present invention provides a system for controlled transgene transcription using chimeric activator and repressor proteins functioning in a novel regulatory network. The target transgene is actively silenced in non-inducing conditions by a novel class of chimeric proteins consisting of the DNA-binding tetracycline repressor fused to distinct multimerized eukaryotic transcriptional repression domains. In the presence of a tetracycline “inducer”, the repressor does not bind to the promoters for both the target gene and for another regulatory gene encoding a transactivator (e.g., GAL4-VP16). Under these inducing conditions, the transactivator activates expression of the target transgene and of its own gene, in an additional autoregulatory positive feedback loop.
摘要翻译：本发明提供使用嵌合活化剂和抑制蛋白在新的调节网络中起作用的受控转基因转录系统。目标转基因在非诱导条件下通过融合到不同的多聚化真核转录抑制结构域的DNA结合四环素阻遏物组成的新一类嵌合蛋白质来主动沉默。在四环素“诱导物”的存在下，阻遏物不与靶基因和编码反式激活因子（例如GAL4-VP16）的另一调控基因的启动子结合。在这些诱导条件下，反式激活剂在另外的自动调节阳性反馈环中激活靶转基因及其自身基因的表达。

2. 发明授权

US5989910A Potent genetic switch allowing regulated gene expression in eukaryotic cells 失效
公开(公告)号：US5989910A
公开(公告)日：1999-11-23
申请号：US128312
申请日：1998-08-03
申请人： Nicolas Mermod , Markus O. Imhof , Philippe Chatellard
发明人： Nicolas Mermod , Markus O. Imhof , Philippe Chatellard
IPC分类号： C12N15/63 , C12N5/00
CPC分类号： C12N15/635
摘要： The present invention provides a system for controlled transgene transcription using chimeric activator and repressor proteins functioning in a novel regulatory network. The target transgene is actively silenced in non-inducing conditions by a novel class of chimeric proteins consisting of the DNA-binding tetracycline repressor fused to distinct multimerized eukaryotic transcriptional repression domains. In the presence of a tetracycline "inducer", the repressor does not bind to the promoters for both the target gene and for another regulatory gene encoding a transactivator (e.g., GAL4-VP16). Under these inducing conditions, the transactivator activates expression of the target transgene and of its own gene, in an additional autoregulatory positive feedback loop.

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