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1. 发明申请

US20070258962A1 Expression Vectors Comprising the Mcmv Ie2 Promoter 有权
标题翻译：包含Mcmv Ie2启动子的表达载体
公开(公告)号：US20070258962A1
公开(公告)日：2007-11-08
申请号：US10548364
申请日：2004-03-10
申请人： Philippe Chatellard , Markus Imhof
发明人： Philippe Chatellard , Markus Imhof
IPC分类号： A61K48/00 , C12N15/63 , C12N5/10 , C12P21/06
CPC分类号： C12N15/85 , C12N2710/16122 , C12N2710/16143 , C12N2830/205 , C12N2830/60
摘要： The invention relates to an expression vector comprising the promoter of the mCMV-IE2 gene, or a functional expression promoting fragment thereof, and/or an enhancer of the mCMV-IE2 gene, or a functional expression enhancing fragment thereof, wherein expression vector does not contain any complete gene of the mCMV.
摘要翻译：本发明涉及包含mCMV-IE2基因的启动子或其功能性表达促进片段和/或mCMV-IE2基因的增强子或其功能性表达增强片段的表达载体，其中表达载体不含有mCMV的任何完整基因。

2. 发明申请

US20080032300A1 Lupac Bifunctional Marker and Its Use in Protein Production 审中-公开
标题翻译： Lupac双功能标记及其在蛋白质生产中的应用
公开(公告)号：US20080032300A1
公开(公告)日：2008-02-07
申请号：US11720560
申请日：2005-12-01
申请人： Philippe Chatellard , Markus Imhof
发明人： Philippe Chatellard , Markus Imhof
IPC分类号： C07H21/04 , C07K14/00 , C12N15/00 , C12N5/00 , C12P21/06 , C12Q1/68
CPC分类号： G01N33/5023 , C07K2319/00 , C12N9/0069 , C12N9/1029
摘要： This invention relates to industrial production of proteins. More specifically, the invention relates to the Lupac surrogate marker, which corresponds to a fusion between luciferase and the puromycin N-acetyl transferase. The invention further relates to the use of Lupac for screening cells for high expression of a protein of interest.
摘要翻译：本发明涉及蛋白质的工业化生产。更具体地，本发明涉及对应于荧光素酶和嘌呤霉素N-乙酰转移酶之间的融合物的Lupac替代标记。本发明还涉及Lupac用于筛选高表达目的蛋白质的细胞的用途。

3. 发明授权

US09051582B2 Expression vectors comprising the mCMV IE2 promoter 有权
标题翻译：包含mCMV IE2启动子的表达载体
公开(公告)号：US09051582B2
公开(公告)日：2015-06-09
申请号：US12872151
申请日：2010-08-31
申请人： Philippe Chatellard , Markus Imhof
发明人： Philippe Chatellard , Markus Imhof
IPC分类号： C12N15/113 , C12N15/11 , C12N15/63 , C12N15/85
CPC分类号： C12N15/85 , C12N2710/16122 , C12N2710/16143 , C12N2830/205 , C12N2830/60
摘要： The invention relates to an expression vector comprising the promoter of the mCMV-IE2 gene, or a functional expression promoting fragment thereof, and/or an enhancer of the mCMV-IE2 gene, or a functional expression enhancing fragment thereof, wherein the expression vector does not contain any complete gene of the mCMV.
摘要翻译：本发明涉及包含mCMV-IE2基因的启动子或其功能性表达促进片段和/或mCMV-IE2基因的增强子或其功能性表达增强片段的表达载体，其中所述表达载体为不含任何完整的mCMV基因。

4. 发明授权

US08133699B2 Minimal DNA sequence acting as a chromatin insulator and its use in protein expression 有权
标题翻译：作为染色质绝缘子的最小DNA序列及其在蛋白质表达中的应用
公开(公告)号：US08133699B2
公开(公告)日：2012-03-13
申请号：US10576509
申请日：2004-10-20
申请人： Philippe Chatellard , Markus Imhof
发明人： Philippe Chatellard , Markus Imhof
IPC分类号： C12P21/06 , C12P21/04 , C07H21/04 , C12N5/00 , C12N5/02
CPC分类号： C07K14/465 , C12N15/63
摘要： The invention relates to expression vectors comprising a DNA sequence of 146 bp capable of acting as chromatin insulator, to host cells containing such vectors, to a method of producing a desired polypeptide by using vectors containing said sequence and to the use of said DNA sequence.
摘要翻译：本发明涉及包含能够作为染色质绝缘体的146bp的DNA序列的表达载体，含有这种载体的宿主细胞，通过使用含有所述序列的载体和所述DNA序列的使用来产生所需多肽的方法。

5. 发明授权

US06340741B1 Potent genetic switch allowing regulated gene expression in eukaryotic cells 失效
标题翻译：有力的基因转换允许在真核细胞中调节基因表达
公开(公告)号：US06340741B1
公开(公告)日：2002-01-22
申请号：US09370683
申请日：1999-08-09
申请人： Nicolas Mermod , Markus O. Imhof , Philippe Chatellard
发明人： Nicolas Mermod , Markus O. Imhof , Philippe Chatellard
IPC分类号： C07K1400
CPC分类号： C12N15/635
摘要： The present invention provides a system for controlled transgene transcription using chimeric activator and repressor proteins functioning in a novel regulatory network. The target transgene is actively silenced in non-inducing conditions by a novel class of chimeric proteins consisting of the DNA-binding tetracycline repressor fused to distinct multimerized eukaryotic transcriptional repression domains. In the presence of a tetracycline “inducer”, the repressor does not bind to the promoters for both the target gene and for another regulatory gene encoding a transactivator (e.g., GAL4-VP16). Under these inducing conditions, the transactivator activates expression of the target transgene and of its own gene, in an additional autoregulatory positive feedback loop.
摘要翻译：本发明提供使用嵌合活化剂和抑制蛋白在新的调节网络中起作用的受控转基因转录系统。目标转基因在非诱导条件下通过融合到不同的多聚化真核转录抑制结构域的DNA结合四环素阻遏物组成的新一类嵌合蛋白质来主动沉默。在四环素“诱导物”的存在下，阻遏物不与靶基因和编码反式激活因子（例如GAL4-VP16）的另一调控基因的启动子结合。在这些诱导条件下，反式激活剂在另外的自动调节阳性反馈环中激活靶转基因及其自身基因的表达。

6. 发明申请

US20110008839A1 Expression Vectors Comprising the MCMV IE2 Promoter 有权
标题翻译：包含MCMV IE2启动子的表达载体
公开(公告)号：US20110008839A1
公开(公告)日：2011-01-13
申请号：US12872151
申请日：2010-08-31
申请人： Philippe Chatellard , Markus Imhof
发明人： Philippe Chatellard , Markus Imhof
IPC分类号： C12P21/00 , C12N15/63 , C12N5/10
CPC分类号： C12N15/85 , C12N2710/16122 , C12N2710/16143 , C12N2830/205 , C12N2830/60
摘要： The invention relates to an expression vector comprising the promoter of the mCMV-IE2 gene, or a functional expression promoting fragment thereof, and/or an enhancer of the mCMV-IE2 gene, or a functional expression enhancing fragment thereof, wherein the expression vector does not contain any complete gene of the mCMV.
摘要翻译：本发明涉及包含mCMV-IE2基因的启动子或其功能性表达促进片段和/或mCMV-IE2基因的增强子或其功能性表达增强片段的表达载体，其中所述表达载体为不含任何完整的mCMV基因。

7. 发明授权

US07824907B2 Expression vectors comprising the mCMV IE2 promoter 有权
标题翻译：包含mCMV IE2启动子的表达载体
公开(公告)号：US07824907B2
公开(公告)日：2010-11-02
申请号：US10548364
申请日：2004-03-10
申请人： Philippe Chatellard , Markus Imhof
发明人： Philippe Chatellard , Markus Imhof
IPC分类号： C12N15/00 , A61K48/00 , A61K39/12
CPC分类号： C12N15/85 , C12N2710/16122 , C12N2710/16143 , C12N2830/205 , C12N2830/60
摘要： The invention relates to an expression vector comprising the promoter of the mCMV-IE2 gene, or a functional expression promoting fragment thereof, and/or an enhancer of the mCMV-IE2 gene, or a functional expression enhancing fragment thereof, wherein expression vector does not contain any complete gene of the mCMV.
摘要翻译：本发明涉及包含mCMV-IE2基因的启动子或其功能性表达促进片段和/或mCMV-IE2基因的增强子或其功能性表达增强片段的表达载体，其中表达载体不含有mCMV的任何完整基因。

8. 发明授权

US07129062B2 Matrix attachment regions and methods for use thereof 有权
标题翻译：基质附着区域及其使用方法
公开(公告)号：US07129062B2
公开(公告)日：2006-10-31
申请号：US10059561
申请日：2002-01-28
申请人： Nicolas Mermod , Monique Zahn-Zabal , Markus Imhof , Philippe Chatellard , Pierre-Alain Girod
发明人： Nicolas Mermod , Monique Zahn-Zabal , Markus Imhof , Philippe Chatellard , Pierre-Alain Girod
IPC分类号： C12P21/06 , C12N15/63 , C12N15/74 , C12N15/00 , C12N5/02 , C07H21/04
CPC分类号： C12N15/85 , C12N2830/46 , C12N2840/203
摘要： The present invention relates to compositions and methods for transfecting eukaryotic cells with nucleic acid vectors. In particular, the invention relates to the uses of Matrix Attachment Region (MAR) elements to increase stable and transient transfection efficiency.
摘要翻译：本发明涉及用核酸载体转染真核细胞的组合物和方法。特别地，本发明涉及基质附着区（MAR）元件用于增加稳定和瞬时转染效率的用途。

9. 发明授权

US5989910A Potent genetic switch allowing regulated gene expression in eukaryotic cells 失效
公开(公告)号：US5989910A
公开(公告)日：1999-11-23
申请号：US128312
申请日：1998-08-03
申请人： Nicolas Mermod , Markus O. Imhof , Philippe Chatellard
发明人： Nicolas Mermod , Markus O. Imhof , Philippe Chatellard
IPC分类号： C12N15/63 , C12N5/00
CPC分类号： C12N15/635
摘要： The present invention provides a system for controlled transgene transcription using chimeric activator and repressor proteins functioning in a novel regulatory network. The target transgene is actively silenced in non-inducing conditions by a novel class of chimeric proteins consisting of the DNA-binding tetracycline repressor fused to distinct multimerized eukaryotic transcriptional repression domains. In the presence of a tetracycline "inducer", the repressor does not bind to the promoters for both the target gene and for another regulatory gene encoding a transactivator (e.g., GAL4-VP16). Under these inducing conditions, the transactivator activates expression of the target transgene and of its own gene, in an additional autoregulatory positive feedback loop.

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