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1. 发明申请

US20110136200A1 Bacterial Glutamine Synthetases and Methods of Use 有权
标题翻译：细菌谷氨酰胺合成酶和使用方法
公开(公告)号：US20110136200A1
公开(公告)日：2011-06-09
申请号：US13027666
申请日：2011-02-15
申请人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
发明人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
IPC分类号： C12N9/00
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包括对应于多核苷酸的多肽。特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

2. 发明授权

US08101389B2 Bacterial glutamine synthetases and methods of use 有权
标题翻译：细菌性谷氨酰胺合成酶及其使用方法
公开(公告)号：US08101389B2
公开(公告)日：2012-01-24
申请号：US13027666
申请日：2011-02-15
申请人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
发明人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
IPC分类号： C12N9/00
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包括对应于多核苷酸的多肽。特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

3. 发明授权

US07910805B2 Bacterial glutamine synthetases and methods of use 有权
标题翻译：细菌性谷氨酰胺合成酶及其使用方法
公开(公告)号：US07910805B2
公开(公告)日：2011-03-22
申请号：US11760602
申请日：2007-06-08
申请人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
发明人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
IPC分类号： C12N15/52 , C12N15/82
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包括对应于多核苷酸的多肽。特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

4. 发明申请

US20090018016A1 BACTERIAL GLUTAMINE SYNTHETASES AND METHODS OF USE 有权
标题翻译：细菌谷氨酰胺合成酶及其使用方法
公开(公告)号：US20090018016A1
公开(公告)日：2009-01-15
申请号：US11760602
申请日：2007-06-08
申请人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
发明人： Nicholas B. Duck , Todd K. Hinson , Vadim Beilinson , Laura Cooper Schouten , Daniel John Tomso
IPC分类号： A01N61/00 , C12N15/11 , C12N15/63 , C12N1/21 , A01P13/00 , A01P21/00 , C12N5/10 , A01H5/00 , C07K14/00
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包括对应于多核苷酸的多肽。特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

5. 发明授权

US08426204B2 Chlamydomonas EPSPS chloroplast transit peptide (CTP) and expression cassettes and transgenic plants utilizing the CTP 有权
标题翻译：衣原体EPSPS叶绿体转运肽（CTP）和表达盒和利用CTP的转基因植物
公开(公告)号：US08426204B2
公开(公告)日：2013-04-23
申请号：US12555175
申请日：2009-09-08
申请人： Philip E. Hammer , Vadim Beilinson , Todd K. Hinson
发明人： Philip E. Hammer , Vadim Beilinson , Todd K. Hinson
IPC分类号： A01H5/00 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8279 , C12N15/8214 , C12N15/8274
摘要： The present invention provides compositions and methods for targeting polypeptides to the chloroplasts of higher plants. Compositions include expression cassettes having a nucleotide sequence encoding a chloroplast targeting peptide (CTP) operably linked to a nucleotide sequence of interest, wherein the CTP is derived from Chlamydomonas sp. Plant transformation vectors, plants and plant cells having the CTP sequences are also encompassed, as well as variants and fragments of the CTP sequences. Methods for expressing a heterologous nucleotide sequence in a plant using the CTP sequences disclosed herein are also provided.
摘要翻译：本发明提供了将多肽靶向高等植物的叶绿体的组合物和方法。组合物包括具有编码与感兴趣的核苷酸序列可操作地连接的叶绿体靶向肽（CTP）的核苷酸序列的表达盒，其中CTP衍生自衣藻属。还包括具有CTP序列的植物转化载体，植物和植物细胞，以及CTP序列的变体和片段。还提供了使用本文公开的CTP序列在植物中表达异源核苷酸序列的方法。

6. 发明申请

US20100071090A1 COMPOSITIONS AND METHODS FOR EXPRESSION OF A HETEROLOGOUS NUCLEOTIDE SEQUENCE IN PLANTS 有权
标题翻译：表达植物中异位核苷酸序列的组合物和方法
公开(公告)号：US20100071090A1
公开(公告)日：2010-03-18
申请号：US12555175
申请日：2009-09-08
申请人： Philip E. Hammer , Vadim Beilinson , Todd K. Hinson
发明人： Philip E. Hammer , Vadim Beilinson , Todd K. Hinson
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8279 , C12N15/8214 , C12N15/8274
摘要： The present invention provides compositions and methods for targeting polypeptides to the chloroplasts of higher plants. Compositions include expression cassettes having a nucleotide sequence encoding a chloroplast targeting peptide (CTP) operably linked to a nucleotide sequence of interest, wherein the CTP is derived from Chlamydomonas sp. Plant transformation vectors, plants and plant cells having the CTP sequences are also encompassed, as well as variants and fragments of the CTP sequences. Methods for expressing a heterologous nucleotide sequence in a plant using the CTP sequences disclosed herein are also provided.
摘要翻译：本发明提供了将多肽靶向高等植物的叶绿体的组合物和方法。组合物包括具有编码与感兴趣的核苷酸序列可操作地连接的叶绿体靶向肽（CTP）的核苷酸序列的表达盒，其中CTP衍生自衣藻属。还包括具有CTP序列的植物转化载体，植物和植物细胞，以及CTP序列的变体和片段。还提供了使用本文公开的CTP序列在植物中表达异源核苷酸序列的方法。

7. 发明申请

US20090241219A1 GDC-2 GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GDC-2基因对抗药性的抵抗
公开(公告)号：US20090241219A1
公开(公告)日：2009-09-24
申请号：US12423926
申请日：2009-04-15
申请人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck
发明人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C12N9/14 , C12P21/02
CPC分类号： C12N9/88 , C12N15/8275 , C12N15/8278
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3 or the nucleotide sequences set forth in SEQ ID NOS:1 and 2.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3所示的氨基酸序列的核苷酸序列或SEQ ID NO：1和2所示的核苷酸序列。

8. 发明授权

US07538262B2 GDC-2 genes conferring herbicide resistance 失效
标题翻译：赋予除草剂抗性的GDC-2基因
公开(公告)号：US07538262B2
公开(公告)日：2009-05-26
申请号：US11185560
申请日：2005-07-20
申请人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck
发明人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck
IPC分类号： A01H5/10 , C12N15/82 , C12N15/52 , C12N15/63
CPC分类号： C12N9/88 , C12N15/8275 , C12N15/8278
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3 or the nucleotide sequences set forth in SEQ ID NOS:1 and 2.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3所示的氨基酸序列的核苷酸序列或SEQ ID NO：1和2所示的核苷酸序列。

9. 发明授权

US07807881B2 Methods to confer herbicide resistance 失效
标题翻译：赋予除草剂抗性的方法
公开(公告)号：US07807881B2
公开(公告)日：2010-10-05
申请号：US11588811
申请日：2006-10-27
申请人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck , Michael G. Koziel
发明人： Philip E. Hammer , Todd K. Hinson , Nicholas B. Duck , Michael G. Koziel
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8274 , C12N9/88 , C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plant cells and bacterial cells are provided. The methods comprise transforming the cells with nucleotide sequences encoding herbicide resistance genes. In particular, herbicide resistance is conferred by expression of proteins with homology to decarboxylase enzymes. Compositions comprise transformed plants, plant tissues, and seeds, as well as transformed bacterial cells.
摘要翻译：提供了赋予对植物细胞和细菌细胞的除草剂抗性的组合物和方法。所述方法包括用编码除草剂抗性基因的核苷酸序列转化细胞。特别地，通过与脱羧酶具有同源性的蛋白质的表达赋予除草剂抗性。组合物包括转化的植物，植物组织和种子，以及转化的细菌细胞。

10. 发明授权

US07504561B2 GDC-1 genes conferring herbicide resistance 失效
标题翻译：赋予除草剂抗性的GDC-1基因
公开(公告)号：US07504561B2
公开(公告)日：2009-03-17
申请号：US11185342
申请日：2005-07-20
申请人： Philip E. Hammer , Todd K. Hinson , Brian Carr , Nicholas B. Duck
发明人： Philip E. Hammer , Todd K. Hinson , Brian Carr , Nicholas B. Duck
IPC分类号： C12N15/82 , C12N15/52
CPC分类号： C12N9/88 , C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding an amino acid sequence shown in SEQ ID NO:3, 6, 8, 11, 19, or 21, or a nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 5, 7, 9, 10, 18, or 20, as well as variants and fragments thereof.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,6,8,11,19或21所示氨基酸序列的核苷酸序列或SEQ ID NO： 1，2，4，5，7，9，10，18或20，以及其变体和片段。

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