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1. 发明授权

US11639498B2 Fusion polymerase and method for using the same 有权
公开(公告)号：US11639498B2
公开(公告)日：2023-05-02
申请号：US16105502
申请日：2018-08-20
申请人： New England Biolabs, Inc.
发明人： Pei-Chung Hsieh , Luo Sun , Thomas C. Evans, Jr. , Theodore B. Davis , Andrew F. Gardner
IPC分类号： C12N9/12 , C12P19/34 , C12N15/10 , C12N15/64 , C12N15/66 , C12N9/22 , C12N9/96 , C12N9/20
摘要： This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.

2. 发明申请

US20220307081A1 Method for Removing and/or Detecting Nucleic Acids Having Mismatched Nucleotides 有权
公开(公告)号：US20220307081A1
公开(公告)日：2022-09-29
申请号：US17825346
申请日：2022-05-26
申请人： New England Biolabs, Inc.
发明人： Andrew F. Gardner
IPC分类号： C12Q1/6855 , C12N9/22 , C12N9/00 , C12Q1/6874
摘要： Provided herein, among other things, are various in vitro methods that involve cleaving dsDNA molecules that comprise a mismatched nucleotide using EndoMS. In some embodiments, the method may comprise ligating a T-tailed double-stranded adapter to A-tailed double-stranded fragments of nucleic acid to produce ligation products that comprise adapter-ligated fragments and double-stranded adapter dimers that comprise a T:T mismatch at the ligation junction and cleaving both strands of the adapter dimers using EndoMS.

3. 发明申请

US20180355328A1 Fusion Polymerase and Method for Using the Same 审中-公开
公开(公告)号：US20180355328A1
公开(公告)日：2018-12-13
申请号：US16105502
申请日：2018-08-20
申请人： New England Biolabs, Inc.
发明人： Pei-Chung Hsieh , Luo Sun , Thomas C. Evans, JR. , Theodore B. Davis , Andrew F. Gardner
IPC分类号： C12N9/12 , C12N9/96 , C12N9/22 , C12N9/20 , C12N15/66 , C12N15/10 , C12P19/34 , C12N15/64
CPC分类号： C12N9/1252 , C07K2319/00 , C12N9/20 , C12N9/22 , C12N9/96 , C12N15/1027 , C12N15/1031 , C12N15/64 , C12N15/66 , C12P19/34 , C12Y207/07007
摘要： This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.

4. 发明申请

US20220282233A1 Cleavage of Single Stranded DNA Having a Modified Nucleotide 有权
公开(公告)号：US20220282233A1
公开(公告)日：2022-09-08
申请号：US17637430
申请日：2020-08-21
申请人： New England Biolabs, Inc.
发明人： Andrew F. Gardner , Kelly M. Zatopek
IPC分类号： C12N9/22 , C12N15/10 , C12Q1/6806 , C12N9/24 , C07K1/10
摘要： Methods are provided that, for example, include (a) combining ssDNA containing a modified nucleotide (e.g., a ssDNA with a modified nucleotide proximate to its 5′ end) with a DNA cleavage enzyme capable of cleaving the ssDNA at the modified nucleotide (e.g., to generate a first ssDNA fragment having a 3′OH and a second ssDNA fragment having the modified nucleotide); wherein the ratio of enzyme to DNA substrate is less than 1:1 molar ratio (m/m); and (b) cleaving at least 95% of the ssDNA at the modified nucleotide. In some embodiments, a method may comprise (a) combining (i) a ssDNA comprising a modified nucleotide (e.g., proximate to its 5′ end) with (ii) a DNA cleavage enzyme capable of cleaving the ssDNA at the modified nucleotide (e.g., to generate (after cleavage) a first ssDNA fragment having a 3′OH and a second ssDNA fragment comprising the modified nucleotide) wherein the ratio of enzyme to DNA substrate is less than 1:1 molar ratio and cleaving at least 95% of the ssDNA at the modified nucleotide. In some embodiments, methods provided herein may include (a) combining (i) a ssDNA (1) immobilized on a substrate and (2) comprising a modified nucleotide with (ii) a ssDNA cleaving enzyme capable of cleaving the ssDNA at the modified nucleotide (e.g., to generate (after cleavage) a first ssDNA fragment having a 3′OH and a second ssDNA fragment comprising the modified nucleotide) ; and (b) cleaving the immobilized ssDNA to release the second single stranded DNA fragment from the substrate. At least 95% (m/m) of an ssDNA comprising a modified nucleotide may be cleaved in less than 60 minutes.

5. 发明授权

US11371088B2 Method for removing and/or detecting nucleic acids having mismatched nucleotides 有权
公开(公告)号：US11371088B2
公开(公告)日：2022-06-28
申请号：US16616631
申请日：2018-06-11
申请人： New England Biolabs, Inc.
发明人： Andrew F. Gardner
IPC分类号： C12Q1/6855 , C12N9/22 , C12N9/00 , C12Q1/6874
摘要： Provided herein, among other things, are various in vitro methods that involve cleaving dsDNA molecules that comprise a mismatched nucleotide using EndoMS. In some embodiments, the method may comprise ligating a T-tailed double-stranded adapter to A-tailed double-stranded fragments of nucleic acid to produce ligation products that comprise adapter-ligated fragments and double-stranded adapter dimers that comprise a T:T mismatch at the ligation junction and cleaving both strands of the adapter dimers using EndoMS.

6. 发明申请

US20210164030A1 Method for Removing and/or Detecting Nucleic Acids Having Mismatched Nucleotides 有权
公开(公告)号：US20210164030A1
公开(公告)日：2021-06-03
申请号：US16616631
申请日：2018-06-11
申请人： New England Biolabs, Inc.
发明人： Andrew F. Gardner
IPC分类号： C12Q1/6855 , C12Q1/6874 , C12N9/22 , C12N9/00
摘要： Provided herein, among other things, are various in vitro methods that involve cleaving dsDNA molecules that comprise a mismatched nucleotide using EndoMS. In some embodiments, the method may comprise ligating a T-tailed double-stranded adapter to A-tailed double-stranded fragments of nucleic acid to produce ligation products that comprise adapter-ligated fragments and double-stranded adapter dimers that comprise a T:T mismatch at the ligation junction and cleaving both strands of the adapter dimers using EndoMS.

7. 发明申请

US20200325533A1 Mapping the Location, Type and Strand of Damaged and/or Mismatched Nucleotides in Double-Stranded DNA 审中-公开
公开(公告)号：US20200325533A1
公开(公告)日：2020-10-15
申请号：US16758190
申请日：2018-08-21
申请人： New England Biolabs, Inc.
发明人： Kelly M. Zatopek , Vladimir Potapov , Jennifer Ong , Laurence Ettwiller , Lixin Chen , Thomas C. Evans, Jr. , Andrew F. Gardner
IPC分类号： C12Q1/6869 , C12Q1/6806
摘要： Providing herein, among other things, is a method comprising incubating a double-stranded nucleic acid having a nick with a nick translating activity, a ligase, and a nucleotide mix comprising at least one modified nucleotide, to generate a product comprising a patch of a newly synthesized strand of a duplex nucleic acid containing a plurality of modified nucleoside monophosphates that are at or adjacent to the site of the nick. In some embodiments, the method may be used to map damaged nucleoside monophosphates in a nucleic acid. Compositions and kits for use in performing the method are also provided.

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