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1. 发明申请

US20080227118A1 Protein Immobilization Method and Quantification Method 审中-公开
标题翻译：蛋白质固定化方法和定量方法
公开(公告)号：US20080227118A1
公开(公告)日：2008-09-18
申请号：US10585985
申请日：2005-01-21
申请人： Naoyuki Kohno , Hitoshi Uemori , Takahiro Nishibu , Kazunari Hirayasu , Yoshiteru Kobayashi , Takashi Yokoyama
发明人： Naoyuki Kohno , Hitoshi Uemori , Takahiro Nishibu , Kazunari Hirayasu , Yoshiteru Kobayashi , Takashi Yokoyama
IPC分类号： G01N33/543 , C07K17/00 , G01N33/567 , C07C315/00 , C07C61/00 , C07C29/00 , G01N33/68
CPC分类号： C07K17/02 , G01N33/543 , G01N33/6896 , G01N2800/2828
摘要： The present invention relates to a method for immobilizing a protein in a sample, which could not easily be immobilized by the conventional immobilization method, to a solid-phase; a method for quantitative determination of protein wherein an effect of inhibitory substance coexisting in a sample prepared using the immobilization method can be reduced; and a rapid and highly precise method for detecting an abnormal PrP and a method for determining BSE using the immobilization method as compared with the conventional method. The present invention provides: “a method for immobilizing a protein to a solid-phase comprising contacting the protein with the solid-phase having hydrophobic surface in the presence of a lower alcohol, and a halogenocarboxylic acid and/or a long chain alkyl sulfate, and an immobilizing reagent solution to be used therefor; a method for quantitative determination of protein comprising contacting a protein-staining solution with the solid-phase immobilized with a protein by the immobilization method, and determining a degree of color development generated thereby; an immunoblotting method wherein the solid-phase immobilized with a protein by the immobilization method is used; and a method for detecting an abnormal PrP a method for determining BSE by using the immobilization method.”
摘要翻译：本发明涉及通过常规固定方法将样品中的蛋白质固定化为固相的方法，用于定量测定蛋白质的方法，其中可以减少使用固定化方法制备的样品中共存的抑制物质的作用; 以及用于检测异常PrP的快速且高精度的方法以及与常规方法相比使用固定方法确定BSE的方法。本发明提供：“将蛋白质固定在固相中的方法，包括在低级醇和卤代羧酸和/或长链烷基硫酸盐的存在下使蛋白质与具有疏水性表面的固相接触，和用于其的固定试剂溶液;一种用于定量测定蛋白质的方法，包括通过固定方法使蛋白质染色溶液与固定有蛋白质的固相接触，并确定由此产生的显色程度;免疫印迹使用通过固定化方法固定有蛋白质的固相的方法;以及通过使用固定化方法来检测异常PrP的方法来测定BSE的方法。

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