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    • 2. 发明申请
    • CARRIER, METHOD AND REAGENT FOR OBTAINING SMALL RNA
    • 载体,用于获得小RNA的方法和试剂
    • US20100311958A1
    • 2010-12-09
    • US12744797
    • 2008-11-27
    • Takahiro NishibuSohei FunaokaYuzo Yamaguchi
    • Takahiro NishibuSohei FunaokaYuzo Yamaguchi
    • C07H1/06B01J20/26
    • C12N15/1006C12N15/111C12N15/113C12N2310/14C12N2310/141C12Q1/6813G01N33/5308C12Q2525/207
    • An object of the present invention is to provide a carrier which enables to obtain a small RNA in high yield yet in high purity, a simple method for obtaining a small RNA and/or a target nucleic acid of small RNA using the same, and a reagent and a kit comprising the above-described carrier.The present invention relates to (1) a carrier for obtaining a small RNA produced by introducing a polymerizable functional group or a chain transfer group to the surface of a core particle, mixing said particle with a polymerizable monomer, then promoting polymerization reaction to form a layer comprising a polymer compound on the surface of said particle, and immobilizing a physiologically active substance having affinity to a small RNA-binding protein on the surface of the particle; (2) a method for obtaining a small RNA, characterized by using the above-described carrier; (3) a reagent for obtaining a small RNA and/or a target nucleic acid of small RNA, comprising the above-described carrier; (4) a reagent kit for obtaining a small RNA and/or a target nucleic acid of small RNA, comprising said reagent; and (5) a method for obtaining a target nucleic acid of small RNA, characterized by using the above-described carrier.
    • 本发明的目的是提供能够以高产率而高纯度获得小RNA的载体,使用该载体获得小RNA的RNA和/或靶核酸的简单方法,以及 试剂和包含上述载体的试剂盒。 本发明涉及(1)一种用于获得通过将可聚合官能团或链转移基团引入核心颗粒表面而获得的小RNA的载体,将所述颗粒与可聚合单体混合,然后促进聚合反应以形成 在所述颗粒的表面上包含聚合物化合物的层,并且将对小的RNA结合蛋白具有亲和力的生理活性物质固定在所述颗粒的表面上; (2)获得小RNA的方法,其特征在于使用上述载体; (3)包含上述载体的用于获得小RNA的小RNA和/或靶核酸的试剂; (4)包含所述试剂的用于获得小RNA的小RNA和/或靶核酸的试剂盒; 和(5)获得小RNA靶核酸的方法,其特征在于使用上述载体。
    • 3. 发明申请
    • Protein Immobilization Method and Quantification Method
    • 蛋白质固定化方法和定量方法
    • US20080227118A1
    • 2008-09-18
    • US10585985
    • 2005-01-21
    • Naoyuki KohnoHitoshi UemoriTakahiro NishibuKazunari HirayasuYoshiteru KobayashiTakashi Yokoyama
    • Naoyuki KohnoHitoshi UemoriTakahiro NishibuKazunari HirayasuYoshiteru KobayashiTakashi Yokoyama
    • G01N33/543C07K17/00G01N33/567C07C315/00C07C61/00C07C29/00G01N33/68
    • C07K17/02G01N33/543G01N33/6896G01N2800/2828
    • The present invention relates to a method for immobilizing a protein in a sample, which could not easily be immobilized by the conventional immobilization method, to a solid-phase; a method for quantitative determination of protein wherein an effect of inhibitory substance coexisting in a sample prepared using the immobilization method can be reduced; and a rapid and highly precise method for detecting an abnormal PrP and a method for determining BSE using the immobilization method as compared with the conventional method. The present invention provides: “a method for immobilizing a protein to a solid-phase comprising contacting the protein with the solid-phase having hydrophobic surface in the presence of a lower alcohol, and a halogenocarboxylic acid and/or a long chain alkyl sulfate, and an immobilizing reagent solution to be used therefor; a method for quantitative determination of protein comprising contacting a protein-staining solution with the solid-phase immobilized with a protein by the immobilization method, and determining a degree of color development generated thereby; an immunoblotting method wherein the solid-phase immobilized with a protein by the immobilization method is used; and a method for detecting an abnormal PrP a method for determining BSE by using the immobilization method.”
    • 本发明涉及通过常规固定方法将样品中的蛋白质固定化为固相的方法, 用于定量测定蛋白质的方法,其中可以减少使用固定化方法制备的样品中共存的抑制物质的作用; 以及用于检测异常PrP的快速且高精度的方法以及与常规方法相比使用固定方法确定BSE的方法。 本发明提供:“将蛋白质固定在固相中的方法,包括在低级醇和卤代羧酸和/或长链烷基硫酸盐的存在下使蛋白质与具有疏水性表面的固相接触, 和用于其的固定试剂溶液;一种用于定量测定蛋白质的方法,包括通过固定方法使蛋白质染色溶液与固定有蛋白质的固相接触,并确定由此产生的显色程度;免疫印迹 使用通过固定化方法固定有蛋白质的固相的方法;以及通过使用固定化方法来检测异常PrP的方法来测定BSE的方法。