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1. 发明申请

US20090288225A1 Novel Aromatic Acyltransferase Genes 有权
标题翻译：新型芳香族酰基转移酶基因
公开(公告)号：US20090288225A1
公开(公告)日：2009-11-19
申请号：US12083581
申请日：2006-10-20
申请人： Naonobu Noda , Kohei Kazuma , Takeshi Sasaki , Koichiro Kogawa , Masahiko Suzuki
发明人： Naonobu Noda , Kohei Kazuma , Takeshi Sasaki , Koichiro Kogawa , Masahiko Suzuki
IPC分类号： A01H1/00 , C07H21/04 , C12N15/63 , C12N5/10 , C07K14/415 , C12P21/06 , A01H5/00
CPC分类号： C12N15/825 , C12N9/1051
摘要： The present invention provides a protein having the amino acid sequence as shown in SEQ ID NO: 2, 4, 6, 8, 10 or 12 or a protein having a modified amino acid sequence thereof and having an activity of transferring an aromatic acyl group to a sugar residue of a flavonoid; a gene, especially cDNA, encoding the protein; and use thereof. For example, by introducing the above gene into a plant expressing hydroxycinnamate 1-O-glucosyltransferase gene, optionally together with a cDNA encoding a protein having the amino acid sequence as shown in SEQ ID NO: 14, 16 or 18 or a protein having an amino acid sequence derived therefrom by modification and having an activity of glucosylating a hydroxyl group at position 1 of hydroxycinnamic acid, and then expressing the introduced gene(s), it is possible to acylate the sugar residue of flavonoids in flowers of the plant to thereby confer a blue color on the flowers.
摘要翻译：本发明提供具有如SEQ ID NO：2,4,6,8,10或12所示氨基酸序列的蛋白质或具有修饰氨基酸序列并具有将芳族酰基转移至类黄酮的糖残基; 编码蛋白质的基因，特别是cDNA; 及其用途。例如，通过将上述基因导入表达羟基肉桂酸酯1-O-葡糖基转移酶基因的植物中，任选与编码具有SEQ ID NO：14,16或18所示氨基酸序列的蛋白质的cDNA一起，或具有通过改性衍生的氨基酸序列，并且具有将羟基肉桂酸的1位的羟基糖基化的活性，然后表达所引入的基因，可以将植物花中的黄酮类化合物的糖残基酰化，由此在花上赋予蓝色。

2. 发明授权

US08110722B2 Aromatic acyltransferase genes 有权
标题翻译：芳香酰基转移酶基因
公开(公告)号：US08110722B2
公开(公告)日：2012-02-07
申请号：US12083581
申请日：2005-10-20
申请人： Naonobu Noda , Kohei Kazuma , Takeshi Sasaki , Koichiro Kogawa , Masahiko Suzuki
发明人： Naonobu Noda , Kohei Kazuma , Takeshi Sasaki , Koichiro Kogawa , Masahiko Suzuki
IPC分类号： A01H5/00 , C12N15/82 , C12N15/52 , C12N15/29 , C07K1/00 , C07K14/415
CPC分类号： C12N15/825 , C12N9/1051
摘要： The present invention provides a protein having the amino acid sequence as shown in SEQ ID NO: 2, 4, 6, 8, 10 or 12 or a protein having a modified amino acid sequence thereof and having an activity of transferring an aromatic acyl group to a sugar residue of a flavonoid; a gene, especially cDNA, encoding the protein; and use thereof. For example, by introducing the above gene into a plant expressing hydroxycinnamate 1-O-glucosyltransferase gene, optionally together with a cDNA encoding a protein having the amino acid sequence as shown in SEQ ID NO: 14, 16 or 18 or a protein having an amino acid sequence derived therefrom by modification and having an activity of glucosylating a hydroxyl group at position 1 of hydroxycinnamic acid, and then expressing the introduced gene(s), it is possible to acylate the sugar residue of flavonoids in flowers of the plant to thereby confer a blue color on the flowers.
摘要翻译：本发明提供具有如SEQ ID NO：2,4,6,8,10或12所示氨基酸序列的蛋白质或具有修饰氨基酸序列并具有将芳族酰基转移至类黄酮的糖残基; 编码蛋白质的基因，特别是cDNA; 及其用途。例如，通过将上述基因导入表达羟基肉桂酸酯1-O-葡糖基转移酶基因的植物中，任选与编码具有SEQ ID NO：14,16或18所示氨基酸序列的蛋白质的cDNA一起，或具有通过改性衍生的氨基酸序列，并且具有将羟基肉桂酸的1位的羟基糖基化的活性，然后表达引入的基因，可以将植物花中的黄酮类的糖残基酰化，由此在花上赋予蓝色。

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