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1. 发明申请

US20120073017A1 METHOD FOR PRODUCING CHRYSANTHEMUM PLANT HAVING PETALS CONTAINING MODIFIED ANTHOCYANIN 有权
标题翻译：生产具有改性蒽醌类化合物的化学植物的方法
公开(公告)号：US20120073017A1
公开(公告)日：2012-03-22
申请号：US13265791
申请日：2010-03-09
申请人： Naonobu Noda , Ryutaro Aida , Sanae Sato , Akemi Ohmiya , Yoshikazu Tanaka
发明人： Naonobu Noda , Ryutaro Aida , Sanae Sato , Akemi Ohmiya , Yoshikazu Tanaka
IPC分类号： C12N15/82 , A01H5/02 , C12N15/113 , A01H5/00
CPC分类号： C12N15/825 , C12N9/1029 , C12N15/8243
摘要： Disclosed are a method for controlling flavonoid synthesis in a chrysanthemum plant or non-chrysanthemum plant by genetic recombination technology using a transcriptional regulatory region useful for altering flower color, a method for modifying anthocyanins, a method for producing a chrysanthemum plant or non-chrysanthemum plant containing modified anthocyanins in the petals thereof, and a chrysanthemum plant or non-chrysanthemum plant, progeny thereof, or vegetatively propagated products, part or tissue thereof transformed with the regulatory region. In the method according to the present invention, an expression vector or expression cassette containing a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase gene, such as a nucleic acid containing the nucleotide sequence indicated in SEQ ID NO. 1, or a transcriptional regulatory region of pansy F3′5′H gene, such as the nucleotide sequence indicated in SEQ ID NO. 15, is used.
摘要翻译：公开了通过使用可用于改变花色的转录调节区域的基因重组技术控制菊花植物或非菊花植物中黄酮类化合物的方法，改性花青素的方法，菊花植物或非菊菊植物的制备方法在其花瓣中含有改性的花青素，以及菊花植物或非菊花植物，其后代或经调节区转化的营养繁殖产物，其部分或组织。在本发明的方法中，含有紫苏花青素3-酰基转移酶基因的转录调节区的表达载体或表达盒，例如含有SEQ ID NO：1所示的核苷酸序列的核酸。 1，或三色堇F3'5'H基因的转录调节区，如SEQ ID NO：1所示的核苷酸序列。 15，被使用。

2. 发明授权

US08871998B2 Method for producing chrysanthemum plant having petals containing modified anthocyanin 有权
标题翻译：生产含有改性花青素花瓣的菊花植物的方法
公开(公告)号：US08871998B2
公开(公告)日：2014-10-28
申请号：US13265791
申请日：2010-03-09
申请人： Naonobu Noda , Ryutaro Aida , Sanae Sato , Akemi Ohmiya , Yoshikazu Tanaka
发明人： Naonobu Noda , Ryutaro Aida , Sanae Sato , Akemi Ohmiya , Yoshikazu Tanaka
IPC分类号： C12N15/09 , C12N15/63 , C12N15/82 , C12N5/04 , C12N9/10
CPC分类号： C12N15/825 , C12N9/1029 , C12N15/8243
摘要： Disclosed are a method for controlling flavonoid synthesis in a chrysanthemum plant or non-chrysanthemum plant by genetic recombination technology using a transcriptional regulatory region useful for altering flower color, a method for modifying anthocyanins, a method for producing a chrysanthemum plant or non-chrysanthemum plant containing modified anthocyanins in the petals thereof, and a chrysanthemum plant or non-chrysanthemum plant, progeny thereof, or vegetatively propagated products, part or tissue thereof transformed with the regulatory region. In the method according to the present invention, an expression vector or expression cassette containing a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase gene, such as a nucleic acid containing the nucleotide sequence indicated in SEQ ID NO. 1, or a transcriptional regulatory region of pansy F3′5′H gene, such as the nucleotide sequence indicated in SEQ ID NO. 15, is used.
摘要翻译：公开了通过使用可用于改变花色的转录调节区域的基因重组技术控制菊花植物或非菊花植物中黄酮类化合物的方法，改性花青素的方法，菊花植物或非菊菊植物的制备方法在其花瓣中含有改性的花青素，以及菊花植物或非菊花植物，其后代或经调节区转化的营养繁殖产物，其部分或组织。在本发明的方法中，含有紫苏花青素3-酰基转移酶基因的转录调节区的表达载体或表达盒，例如含有SEQ ID NO：1所示的核苷酸序列的核酸。 1，或三色堇F3'5'H基因的转录调节区，如SEQ ID NO：1所示的核苷酸序列。 15，被使用。

3. 发明申请

US20120096589A1 METHOD FOR PRODUCTION OF CHRYSANTHEMUM PLANT HAVING DELPHINIDIN-CONTAINING PETALS 有权
标题翻译：用于生产含有含有蝶啶含量的石蜡的植物的方法
公开(公告)号：US20120096589A1
公开(公告)日：2012-04-19
申请号：US13265688
申请日：2010-03-09
申请人： Naonobu Noda , Ryutaro Aida , Sanae Sato , Akemi Ohmiya , Yoshikazu Tanaka
发明人： Naonobu Noda , Ryutaro Aida , Sanae Sato , Akemi Ohmiya , Yoshikazu Tanaka
IPC分类号： A01H5/02 , A01H5/00 , C12N5/04 , A01H1/00
CPC分类号： C12N15/8243 , A01H5/02 , A01H6/1424 , C12N9/0071 , C12N15/825
摘要： Disclosed are: a method for producing a chrysanthemum plant having delphinidin-containing petals using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene; and a chrysanthemum plant, a progeny or a vegetative proliferation product of the plant, or a part or a tissue of the plant, the progeny or the vegetative proliferation product, and particularly a petal or a cut flower of the plant. In the method for producing a chrysanthemum plant having delphinidin-containing petals, a flavonoid 3′,5′-hydroxylase (F3′5′H) is caused to be expressed in a chrysanthemum plant using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene.
摘要翻译：公开了一种使用菊花衍生的黄烷酮3-羟化酶（F3H）基因的转录调节区域产生具有花翠素的花瓣的菊花植物的方法。菊花植物，植物的后代或营养增殖产物，植物的一部分或组织，子代或营养增殖产物，特别是植物的花瓣或切花。在具有含花翠素的花瓣的菊花植物的制造方法中，使用菊花衍生的黄烷酮的转录调节区域在菊花植物中表达黄酮3'，5'-羟化酶（F3'5'H） 3-羟化酶（F3H）基因。

4. 发明申请

US20090288225A1 Novel Aromatic Acyltransferase Genes 有权
标题翻译：新型芳香族酰基转移酶基因
公开(公告)号：US20090288225A1
公开(公告)日：2009-11-19
申请号：US12083581
申请日：2006-10-20
申请人： Naonobu Noda , Kohei Kazuma , Takeshi Sasaki , Koichiro Kogawa , Masahiko Suzuki
发明人： Naonobu Noda , Kohei Kazuma , Takeshi Sasaki , Koichiro Kogawa , Masahiko Suzuki
IPC分类号： A01H1/00 , C07H21/04 , C12N15/63 , C12N5/10 , C07K14/415 , C12P21/06 , A01H5/00
CPC分类号： C12N15/825 , C12N9/1051
摘要： The present invention provides a protein having the amino acid sequence as shown in SEQ ID NO: 2, 4, 6, 8, 10 or 12 or a protein having a modified amino acid sequence thereof and having an activity of transferring an aromatic acyl group to a sugar residue of a flavonoid; a gene, especially cDNA, encoding the protein; and use thereof. For example, by introducing the above gene into a plant expressing hydroxycinnamate 1-O-glucosyltransferase gene, optionally together with a cDNA encoding a protein having the amino acid sequence as shown in SEQ ID NO: 14, 16 or 18 or a protein having an amino acid sequence derived therefrom by modification and having an activity of glucosylating a hydroxyl group at position 1 of hydroxycinnamic acid, and then expressing the introduced gene(s), it is possible to acylate the sugar residue of flavonoids in flowers of the plant to thereby confer a blue color on the flowers.
摘要翻译：本发明提供具有如SEQ ID NO：2,4,6,8,10或12所示氨基酸序列的蛋白质或具有修饰氨基酸序列并具有将芳族酰基转移至类黄酮的糖残基; 编码蛋白质的基因，特别是cDNA; 及其用途。例如，通过将上述基因导入表达羟基肉桂酸酯1-O-葡糖基转移酶基因的植物中，任选与编码具有SEQ ID NO：14,16或18所示氨基酸序列的蛋白质的cDNA一起，或具有通过改性衍生的氨基酸序列，并且具有将羟基肉桂酸的1位的羟基糖基化的活性，然后表达所引入的基因，可以将植物花中的黄酮类化合物的糖残基酰化，由此在花上赋予蓝色。

5. 发明授权

US09074215B2 Method for production of chrysanthemum plant having delphinidin-containing petals 有权
标题翻译：生产含有花翠素的菊花植物的方法
公开(公告)号：US09074215B2
公开(公告)日：2015-07-07
申请号：US13265688
申请日：2010-03-09
申请人： Naonobu Noda , Ryutaro Aida , Sanae Sato , Akemi Ohmiya , Yoshikazu Tanaka
发明人： Naonobu Noda , Ryutaro Aida , Sanae Sato , Akemi Ohmiya , Yoshikazu Tanaka
IPC分类号： C12N15/82 , C12N9/02 , A01H5/02
CPC分类号： C12N15/8243 , A01H5/02 , A01H6/1424 , C12N9/0071 , C12N15/825
摘要： Disclosed are: a method for producing a chrysanthemum plant having delphinidin-containing petals using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene; and a chrysanthemum plant, a progeny or a vegetative proliferation product of the plant, or a part or a tissue of the plant, the progeny or the vegetative proliferation product, and particularly a petal or a cut flower of the plant. In the method for producing a chrysanthemum plant having delphinidin-containing petals, a flavonoid 3′,5′-hydroxylase (F3′5′H) is caused to be expressed in a chrysanthemum plant using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene.
摘要翻译：公开了一种使用菊花衍生的黄烷酮3-羟化酶（F3H）基因的转录调节区域产生具有花翠素的花瓣的菊花植物的方法。菊花植物，植物的后代或营养增殖产物，植物的一部分或组织，子代或营养增殖产物，特别是植物的花瓣或切花。在具有含花翠素的花瓣的菊花植物的制造方法中，使用菊花衍生的黄烷酮的转录调节区域在菊花植物中表达黄酮3'，5'-羟化酶（F3'5'H） 3-羟化酶（F3H）基因。

6. 发明授权

US08110722B2 Aromatic acyltransferase genes 有权
标题翻译：芳香酰基转移酶基因
公开(公告)号：US08110722B2
公开(公告)日：2012-02-07
申请号：US12083581
申请日：2005-10-20
申请人： Naonobu Noda , Kohei Kazuma , Takeshi Sasaki , Koichiro Kogawa , Masahiko Suzuki
发明人： Naonobu Noda , Kohei Kazuma , Takeshi Sasaki , Koichiro Kogawa , Masahiko Suzuki
IPC分类号： A01H5/00 , C12N15/82 , C12N15/52 , C12N15/29 , C07K1/00 , C07K14/415
CPC分类号： C12N15/825 , C12N9/1051
摘要： The present invention provides a protein having the amino acid sequence as shown in SEQ ID NO: 2, 4, 6, 8, 10 or 12 or a protein having a modified amino acid sequence thereof and having an activity of transferring an aromatic acyl group to a sugar residue of a flavonoid; a gene, especially cDNA, encoding the protein; and use thereof. For example, by introducing the above gene into a plant expressing hydroxycinnamate 1-O-glucosyltransferase gene, optionally together with a cDNA encoding a protein having the amino acid sequence as shown in SEQ ID NO: 14, 16 or 18 or a protein having an amino acid sequence derived therefrom by modification and having an activity of glucosylating a hydroxyl group at position 1 of hydroxycinnamic acid, and then expressing the introduced gene(s), it is possible to acylate the sugar residue of flavonoids in flowers of the plant to thereby confer a blue color on the flowers.
摘要翻译：本发明提供具有如SEQ ID NO：2,4,6,8,10或12所示氨基酸序列的蛋白质或具有修饰氨基酸序列并具有将芳族酰基转移至类黄酮的糖残基; 编码蛋白质的基因，特别是cDNA; 及其用途。例如，通过将上述基因导入表达羟基肉桂酸酯1-O-葡糖基转移酶基因的植物中，任选与编码具有SEQ ID NO：14,16或18所示氨基酸序列的蛋白质的cDNA一起，或具有通过改性衍生的氨基酸序列，并且具有将羟基肉桂酸的1位的羟基糖基化的活性，然后表达引入的基因，可以将植物花中的黄酮类的糖残基酰化，由此在花上赋予蓝色。

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