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1. 发明申请

US20060040339A1 Method of constructing a calibration curve in microorganism assay 审中-公开
标题翻译：在微生物测定中构建校准曲线的方法
公开(公告)号：US20060040339A1
公开(公告)日：2006-02-23
申请号：US10919290
申请日：2004-08-17
申请人： Naoki Fukui , Megumi Akamatsu , Seiichiro Miyahara , Chiaki Okumura , Toshimi Murata , Yoshihisa Amano
发明人： Naoki Fukui , Megumi Akamatsu , Seiichiro Miyahara , Chiaki Okumura , Toshimi Murata , Yoshihisa Amano
IPC分类号： G06F19/00 , C12Q1/04
CPC分类号： C12Q1/06
摘要： A method of constructing a calibration curve in a determination of microbial counts is provided. An immunoassay of the present invention is characterized in that samples of a specimen material are prepared so that microbial counts therein are altered.
摘要翻译：提供了一种在确定微生物计数中构建校准曲线的方法。本发明的免疫测定法的特征在于，准备样品的样品，使其中的微生物计数改变。

2. 发明申请

US20080197023A1 Method Of Measuring the Number Of Bacteria, Device Of Measuring the Number Of Bacteria and Cell Used In the Device 有权
标题翻译：测量细菌数量的方法，测量装置中使用的细菌数和细胞数量的装置
公开(公告)号：US20080197023A1
公开(公告)日：2008-08-21
申请号：US11573081
申请日：2005-06-16
申请人： Seiichiro Miyahara , Chiaki Okumura , Naoki Fukui , Megumi Akamatsu
发明人： Seiichiro Miyahara , Chiaki Okumura , Naoki Fukui , Megumi Akamatsu
IPC分类号： G01N27/26 , G01N33/487
CPC分类号： G01N15/1031 , C12Q1/06 , C12Q1/10
摘要： A method of measuring the number of bacteria according to the present invention includes step (a) through step (d). First, in step (a), a to-be-measured sample (specimen) including a predetermined bacterial strain (such as Escherichia coli or coliform bacteria) is added to a predetermined medium (such as a medium used for a specific enzyme substrate culture medium method). In step (b), a current value flowing through the medium added with the sample is measured with an oxygen electrode at a predetermined temperature and at a predetermined constant voltage. In step (c), time required is measured for the current value that has decreased temporarily after starting the measurement of step (b) to increase thereafter to exceed a predetermined threshold value. In step (d), the initial number of bacteria of the bacterial strain included in the sample is calculated based on the time required.
摘要翻译：根据本发明的测量细菌数量的方法包括步骤（a）至步骤（d）。首先，在步骤（a）中，向规定的培养基（例如用于特定的酶底物培养基的培养基）中加入包含规定菌种（例如大肠杆菌或大肠菌群）的待测样品（标本）中等方法）。在步骤（b）中，用氧电极在预定温度和预定的恒定电压下测量流过添加了样品的介质的电流值。在步骤（c）中，测量在开始步骤（b）的测量之后临时减小的电流值随后增加到超过预定阈值的时间。在步骤（d）中，根据所需的时间计算样品中包含的细菌菌株的初始菌数。

3. 发明申请

US20080057535A1 Method of Measuring the Number of Microorganisms and Apparatus for Measuring the Number of Microorganisms 审中-公开
标题翻译：微生物数量测定方法及微生物数量测定装置
公开(公告)号：US20080057535A1
公开(公告)日：2008-03-06
申请号：US11791312
申请日：2005-11-22
申请人： Megumi Akamatsu , Chiaki Okumura , Seiichiro Miyahara , Naoki Fukui
发明人： Megumi Akamatsu , Chiaki Okumura , Seiichiro Miyahara , Naoki Fukui
IPC分类号： C12Q1/06 , C12M1/34
CPC分类号： C12Q1/06
摘要： Various settings used in a process proceeding in line with a flow chart are reset. An oxygen current is measured. A slope of the oxygen current is obtained. A determination is made as to whether or not the slope can be considered to become stable. A first measuring time is determined. In order to determine the stability of the slope, the oxygen current is sequentially measured and the slope of the oxygen current is updated until the slope is determined to become stable. When the slope is determined to become stable, the first measuring time is determined. When the slope is not determined to become stable, the oxygen current is further measured.
摘要翻译：根据流程图进行的过程中使用的各种设置被重置。测量氧气电流。得到氧气电流的斜率。确定斜率是否可以被认为变得稳定。确定第一测量时间。为了确定斜率的稳定性，依次测量氧气电流，并更新氧气电流的斜率，直到确定斜率变得稳定为止。当确定斜率变得稳定时，确定第一测量时间。当斜率未确定为稳定时，进一步测量氧气电流。

4. 发明授权

US07955493B2 Method of measuring the number of bacteria, device of measuring the number of bacteria and cell used in the device 有权
标题翻译：测量细菌数量的方法，测量装置中使用的细菌和细胞数量的装置
公开(公告)号：US07955493B2
公开(公告)日：2011-06-07
申请号：US11573081
申请日：2005-06-16
申请人： Seiichiro Miyahara , Chiaki Okumura , Naoki Fukui , Megumi Akamatsu
发明人： Seiichiro Miyahara , Chiaki Okumura , Naoki Fukui , Megumi Akamatsu
IPC分类号： C12M1/34
CPC分类号： G01N15/1031 , C12Q1/06 , C12Q1/10
摘要： A method of measuring the number of bacteria according to the present invention includes step (a) through step (d). First, in step (a), a to-be-measured sample (specimen) including a predetermined bacterial strain (such as Escherichia coli or coliform bacteria) is added to a predetermined medium (such as a medium used for a specific enzyme substrate culture medium method). In step (b), a current value flowing through the medium added with the sample is measured with an oxygen electrode at a predetermined temperature and at a predetermined constant voltage. In step (c), time required is measured for the current value that has decreased temporarily after starting the measurement of step (b) to increase thereafter to exceed a predetermined threshold value. In step (d), the initial number of bacteria of the bacterial strain included in the sample is calculated based on the time required.
摘要翻译：根据本发明的测量细菌数量的方法包括步骤（a）至步骤（d）。首先，在步骤（a）中，向规定的培养基（例如用于特定的酶底物培养基的培养基）中加入包含规定菌种（例如大肠杆菌或大肠菌群）的待测样品（标本）中等方法）。在步骤（b）中，用氧电极在预定温度和预定的恒定电压下测量流过添加了样品的介质的电流值。在步骤（c）中，测量在开始步骤（b）的测量之后临时减小的电流值随后增加到超过预定阈值的时间。在步骤（d）中，根据所需的时间计算样品中包含的细菌菌株的初始菌数。

5. 发明授权

US07198906B2 Drug sensitivity measuring method 失效
标题翻译：药物敏感性测定方法
公开(公告)号：US07198906B2
公开(公告)日：2007-04-03
申请号：US10221800
申请日：2001-03-19
申请人： Katsuhiko Machida , Sadayori Hoshina , Takashi Ushida , Junichiro Arai , Hideo Katayama , Chiaki Okumura , Yoshihisa Amano
发明人： Katsuhiko Machida , Sadayori Hoshina , Takashi Ushida , Junichiro Arai , Hideo Katayama , Chiaki Okumura , Yoshihisa Amano
IPC分类号： C12Q1/18 , C12M1/34
CPC分类号： C12Q1/025 , C12Q1/18
摘要： Disclosed herein is a method for determining the drug sensitivity of a microbe which comprises pouring a microbial suspension into each of the compartments or wells at a position which is in the vicinity of an electrode for measuring dissolved oxygen concentration, measuring current from each electrode at a second time interval for a third time period, each time is obtained based upon each of the measured currents at which the maximum current is obtained, obtaining each current value within a fourth time period which starts from the time at which the maximum current is obtained, detecting drug sensitivity based upon the variation condition of each current value during the fourth time period. The method allows rapid drug susceptibility measurements.
摘要翻译：本文公开了一种用于测定微生物药物敏感性的方法，该方法包括将微生物悬浮液在位于用于测量溶解氧浓度的电极附近的位置处的每个隔室或孔中倒入，测量来自每个电极的电流第三时间段的第二时间间隔，基于获得最大电流的每个测量电流获得每次，获得从获得最大电流的时间开始的第四时间段内的每个电流值，基于第四时间段内的每个电流值的变化条件来检测药物敏感性。该方法允许快速的药物敏感性测量。

6. 发明授权

US07081353B2 Drug susceptibility measurement method and apparatus thereof 失效
标题翻译：药敏试验方法及其装置
公开(公告)号：US07081353B2
公开(公告)日：2006-07-25
申请号：US10221796
申请日：2001-03-20
申请人： Katsuhiko Machida , Sadayori Hoshina , Takashi Ushida , Junichiro Arai , Hideo Katayama , Chiaki Okumura , Yoshihisa Amano
发明人： Katsuhiko Machida , Sadayori Hoshina , Takashi Ushida , Junichiro Arai , Hideo Katayama , Chiaki Okumura , Yoshihisa Amano
IPC分类号： C12M1/34 , C12Q1/18
CPC分类号： C12Q1/06
摘要： A method and apparatus wherein measurement current values output from a first oxygen sensor and a second oxygen sensor are time sequentially measured. Each moving average value is calculated from each sequential measurement current value. Each time differential value is calculated from the pair of each calculated moving average values, by least squares approximation. Then, drug susceptibility is measured based upon each calculated time differential value, so that drug the susceptibility measurement is performed quickly or accurately.
摘要翻译：其中从第一氧传感器和第二氧传感器输出的测量电流值被时间依次测量的方法和装置。每个移动平均值由每个顺序测量电流值计算。每次通过最小平方近似计算每个计算的移动平均值对的差分值。然后，基于每个计算的时间微分值测量药物敏感性，使得药物敏感性测量快速或准确地进行。

7. 发明授权

US5563194A Propylene resin composition 失效
标题翻译：丙烯树脂组合物
公开(公告)号：US5563194A
公开(公告)日：1996-10-08
申请号：US514138
申请日：1995-08-11
申请人： Kazuyuki Watanabe , Chiaki Okumura
发明人： Kazuyuki Watanabe , Chiaki Okumura
IPC分类号： C08K3/34 , C08K5/00 , C08K5/52 , C08K5/521 , C08L23/00 , C08L23/08 , C08L23/10 , C08L23/12 , C08L23/16 , C08L53/00
CPC分类号： C08K3/34 , C08L23/10 , C08L23/12 , C08L53/00 , C08K5/0083 , C08L2205/02 , C08L2205/035 , C08L23/0815 , C08L23/16
摘要： A propylene resin composition comprising: a polypropylelne having specific physical properties, 25.about.40% by weight; a propylene-ethylene block copolymer, 25.about.45% by weight; an ethylene-propylene rubber, 5.about.15% by weight; an ethylene-.alpha.-olefin copolymer, 5-15% by weight; and a talc, 5-30% by weight; is superior in all of the following properties concurrently: rigidity, heat resistance, impact resistance, surface hardness, etc.; and is suited for use in machine parts such as automobile parts; electric and electronic parts; packaging materials, engineering plastic substitutes, etc.
摘要翻译：丙烯树脂组合物，其包含：具有特定物理性质的聚丙烯，40重量％的差异; 丙烯 - 乙烯嵌段共聚物，25重量％; 乙烯 - 丙烯橡胶，5％重量比; 5-15重量％的乙烯-α-烯烃共聚物; 和5-30重量％的滑石粉; 在所有以下性能方面同时优越：刚性，耐热性，抗冲击性，表面硬度等; 适用于汽车零部件等机械部件; 电气电子配件; 包装材料，工程塑料替代品等

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