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1. 发明授权

US08114611B2 Affinity chromatography matrices and methods of making and using the same 有权
标题翻译：亲和层析矩阵及其使用方法
公开(公告)号：US08114611B2
公开(公告)日：2012-02-14
申请号：US12916978
申请日：2010-11-01
申请人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
发明人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
IPC分类号： G01N33/53
CPC分类号： G01N33/54313 , B01D15/3809 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/315 , C12N11/08 , G01N33/54353
摘要： The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.
摘要翻译：本发明提供了将蛋白质配体与固体支持物偶联的方法。本发明还提供亲和层析基质和使用亲和层析基质纯化靶分子的方法。

2. 发明申请

US20110105730A1 AFFINITY CHROMATOGRAPHY MATRICES AND METHODS OF MAKING AND USING THE SAME 有权
标题翻译：亲和色谱法及其制备和使用方法
公开(公告)号：US20110105730A1
公开(公告)日：2011-05-05
申请号：US12916978
申请日：2010-11-01
申请人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
发明人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
IPC分类号： C07K1/22 , C07K1/36 , C07H1/06 , C07K17/10
CPC分类号： G01N33/54313 , B01D15/3809 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/315 , C12N11/08 , G01N33/54353
摘要： The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.
摘要翻译：本发明提供了将蛋白质配体与固体支持物偶联的方法。本发明还提供亲和层析基质和使用亲和层析基质纯化靶分子的方法。

3. 发明授权

US07833723B2 Affinity chromatography matrices and methods of making and using the same 有权
公开(公告)号：US07833723B2
公开(公告)日：2010-11-16
申请号：US11650093
申请日：2007-01-05
申请人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
发明人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
IPC分类号： G01N33/53
CPC分类号： G01N33/54313 , B01D15/3809 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/315 , C12N11/08 , G01N33/54353
摘要： The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

4. 发明申请

US20070207500A1 Affinity chromatography matrices and methods of making and using the same 有权
标题翻译：亲和层析矩阵及其使用方法
公开(公告)号：US20070207500A1
公开(公告)日：2007-09-06
申请号：US11650093
申请日：2007-01-05
申请人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
发明人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
IPC分类号： G01N33/53 , C07K14/47
CPC分类号： G01N33/54313 , B01D15/3809 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/315 , C12N11/08 , G01N33/54353
摘要： The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.
摘要翻译：本发明提供了将蛋白质配体与固体支持物偶联的方法。本发明还提供亲和层析基质和使用亲和层析基质纯化靶分子的方法。

5. 发明授权

US07846682B2 Affinity chromatography matrices and methods of making and using the same 有权
标题翻译：亲和层析矩阵及其使用方法
公开(公告)号：US07846682B2
公开(公告)日：2010-12-07
申请号：US12590726
申请日：2009-11-13
申请人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
发明人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Chen Wang
IPC分类号： G01N33/53
CPC分类号： G01N33/54313 , B01D15/3809 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/315 , C12N11/08 , G01N33/54353
摘要： The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.
摘要翻译：本发明提供了将蛋白质配体与固体支持物偶联的方法。本发明还提供亲和层析基质和使用亲和层析基质纯化靶分子的方法。

6. 发明申请

US20100063261A1 Affinity chromatography matrices and methods of making and using the same 有权
公开(公告)号：US20100063261A1
公开(公告)日：2010-03-11
申请号：US12590726
申请日：2009-11-13
申请人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Cheng Wang
发明人： Nanying Bian , John Charkoudian , Neil Soice , Joaquin Umana , Cheng Wang
IPC分类号： C07K14/00 , C07K1/22
CPC分类号： G01N33/54313 , B01D15/3809 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/315 , C12N11/08 , G01N33/54353
摘要： The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

7. 发明申请

US20070212540A1 Asymmetric porous adsorptive bead 有权
标题翻译：不对称多孔吸附珠
公开(公告)号：US20070212540A1
公开(公告)日：2007-09-13
申请号：US11520848
申请日：2006-09-13
申请人： Kwok-Shun Cheng , Senthilkumar Ramaswamy , Chen Wang , Nanying Bian , Brian Gagnon , Joaquin Umana , Dennis Aquino , Neil Soice , Lyddiatt Andrew
发明人： Kwok-Shun Cheng , Senthilkumar Ramaswamy , Chen Wang , Nanying Bian , Brian Gagnon , Joaquin Umana , Dennis Aquino , Neil Soice , Lyddiatt Andrew
IPC分类号： B32B1/00 , B01D15/08 , B01D39/00
CPC分类号： C07K16/00 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28019 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3212 , B01J20/3244 , B01J20/3274 , B01J20/328 , B01J20/3285 , B01J20/3293 , B01J39/26 , Y10T428/2982
摘要： The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.
摘要翻译：本发明涉及适用于分离应用的非对称色谱介质，特别是作为填充床，流化床或磁化床层析介质。在某些实施方案中，不对称色谱介质包含具有至少两个不同的，受控的孔径分布的不对称颗粒，优选珠粒。优选地，不同孔径分布中的一个在颗粒的内部区域中，另一个位于颗粒的外部区域或涂层中。这些不同的孔径分布可以用均匀的或替代地独特的官能团或官能团的混合物进行改性。本发明允许通过提供不同的内部区域，优选以珠粒的形式和不同的外部区域，优选以珠粒上的涂层的形式来控制不对称多孔颗粒内的孔径分布。

8. 发明申请

US20070069408A1 Method and apparatus for making porous agarose beads 有权
标题翻译：制备多孔琼脂糖珠的方法和设备
公开(公告)号：US20070069408A1
公开(公告)日：2007-03-29
申请号：US11520775
申请日：2006-09-13
申请人： Kwok-Shun Cheng , Senthilkumar Ramaswamy , Nanying Bian , Brian Gagnon , Joaquin Umana , Neil Soice
发明人： Kwok-Shun Cheng , Senthilkumar Ramaswamy , Nanying Bian , Brian Gagnon , Joaquin Umana , Neil Soice
IPC分类号： B01J13/04 , B28B1/54
CPC分类号： B01J20/24 , B01J13/04 , B01J20/28009 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3204 , B01J20/3242 , B01J20/3244 , B01J20/3272 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J20/3293 , B01J2220/4825
摘要： The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/ or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.
摘要翻译：本发明涉及用于形成琼脂糖或核心琼脂糖珠的方法和装置。该方法包括将琼脂糖溶解/凝胶化在合适的液体中，将其与疏水性液体混合以形成乳液，并将该乳液保持在等于或大于琼脂糖凝胶点的温度，将其通过静态混合器以产生琼脂糖滴并在第二浴疏水性液体中固化琼脂糖滴。然后可以将珠子洗涤并使用或进一步加工以使琼脂糖交联和/或在琼脂糖上添加各种功能。用于固化琼脂糖滴的另一种方法是使用热交换器在离开静态混合器之后连续冷却流。对于“核心”珠粒，使用类似的方法，除了核心，优选以珠粒形式，在其进入第一疏水性液体之前将其引入琼脂糖，使得琼脂糖在芯上形成涂层。可以使用与琼脂糖珠（由该方法或另一方法制备）或核心琼脂糖（由该方法或另一方法制备）相似的方法将多层琼脂糖添加到现有珠粒上。还公开了一种用于运行该过程的装置。

9. 发明申请

US20100227015A1 Method and apparatus for making porous agarose beads 有权
公开(公告)号：US20100227015A1
公开(公告)日：2010-09-09
申请号：US12660708
申请日：2010-03-02
申请人： Kwok-Shun Cheng , Senthilkumar Ramaswamy , Nanying Bian , Brian Gagnon , Joaquin Umana , Neil P. Soice
发明人： Kwok-Shun Cheng , Senthilkumar Ramaswamy , Nanying Bian , Brian Gagnon , Joaquin Umana , Neil P. Soice
IPC分类号： B01J13/04
CPC分类号： B01J20/24 , B01J13/04 , B01J20/28009 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3204 , B01J20/3242 , B01J20/3244 , B01J20/3272 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J20/3293 , B01J2220/4825
摘要： The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.

10. 发明授权

US07678302B2 Method and apparatus for making porous agarose beads 有权
标题翻译：制备多孔琼脂糖珠的方法和设备
公开(公告)号：US07678302B2
公开(公告)日：2010-03-16
申请号：US11520775
申请日：2006-09-13
申请人： Kwok-Shun Cheng , Senthilkumar Ramaswamy , Nanying Bian , Brian Gagnon , Joaquin Umana , Neil P. Soice
发明人： Kwok-Shun Cheng , Senthilkumar Ramaswamy , Nanying Bian , Brian Gagnon , Joaquin Umana , Neil P. Soice
IPC分类号： B29B9/00
CPC分类号： B01J20/24 , B01J13/04 , B01J20/28009 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3204 , B01J20/3242 , B01J20/3244 , B01J20/3272 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J20/3293 , B01J2220/4825
摘要： The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.
摘要翻译：本发明涉及用于形成琼脂糖或核心琼脂糖珠的方法和装置。该方法包括将琼脂糖溶解/凝胶化在合适的液体中，将其与疏水性液体混合以形成乳液，并将该乳液保持在等于或大于琼脂糖凝胶点的温度，将其通过静态混合器以产生琼脂糖滴并在第二浴疏水性液体中固化琼脂糖滴。然后可以将珠子洗涤并使用或进一步加工以使琼脂糖交联和/或在琼脂糖上添加各种功能。用于固化琼脂糖滴的另一种方法是通过使用热交换器在离开静态混合器之后连续冷却流。对于“核心”珠粒，使用类似的方法，除了核心，优选以珠粒形式，在其进入第一疏水性液体之前将其引入琼脂糖，使得琼脂糖在芯上形成涂层。可以使用与琼脂糖珠（由该方法或另一方法制备）或核心琼脂糖（由该方法或另一方法制备）相似的方法将多层琼脂糖添加到现有珠粒上。还公开了一种用于运行该过程的装置。

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