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1. 发明授权

US5364591A Device for moving a target-bearing solid through a liquid for detection while being contained 失效
标题翻译：用于通过液体移动目标轴承固体以用于检测同时被容纳的装置
公开(公告)号：US5364591A
公开(公告)日：1994-11-15
申请号：US891517
申请日：1992-06-01
申请人： Nancy F. Green , Thomas J. Cummins , Fred T. Oakes
发明人： Nancy F. Green , Thomas J. Cummins , Fred T. Oakes
IPC分类号： C12M1/00 , A61B10/00 , B01J19/00 , B01L3/00 , B01L3/14 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N33/537 , G01N33/546
CPC分类号： B01L3/502 , A61B10/0096 , B01J19/0046 , B01J2219/00283 , B01J2219/00335 , B01J2219/00346 , B01J2219/00511 , B01J2219/00722 , B01L2300/0672 , B01L2300/087 , B01L2400/0677 , B01L2400/0683 , B01L3/5082 , B01L3/5635 , C40B40/06 , C40B60/14 , Y10S435/81 , Y10S436/807 , Y10S436/809 , Y10S436/81
摘要： There are described a device and method for doing confined reactions such as PCR amplification and detection, wherein solids (e.g., beads) used to obtain separation between bound and "free" label reagents, are transferred from region to region, specifically through a wash liquid so as to wash off the "free" label reagent from the solids. Separate chambers have dividers that are overcome by piercing or by liquification, to create passageways for the transfer of the solids. The passageways remain contained within the device.
摘要翻译：描述了用于进行限制性反应（例如PCR扩增和检测）的装置和方法，其中用于获得结合的和“游离的”标记试剂之间的分离的固体（例如，珠）从区域转移到区域，特别是通过洗涤液以便从固体中清除“游离”标记试剂。分隔室具有通过穿透或液化来克服的分隔件，以产生用于固体传递的通道。通道仍然包含在设备内。

2. 发明授权

US5567810A Nuclease resistant compounds 失效
标题翻译：核酸酶抗性化合物
公开(公告)号：US5567810A
公开(公告)日：1996-10-22
申请号：US456017
申请日：1995-05-31
申请人： Alexander L. Weis , Fred T. Oakes , Frederick H. Hausheer , Paul F. Cavanaugh, Jr. , Patricia S. Moskwa
发明人： Alexander L. Weis , Fred T. Oakes , Frederick H. Hausheer , Paul F. Cavanaugh, Jr. , Patricia S. Moskwa
IPC分类号： C07H21/00 , A61K31/045 , A61K31/715 , C07C31/20
CPC分类号： C07H21/00
摘要： Compounds, compositions and methods for inhibiting gene expression are disclosed. The compounds comprise oligonucleotide sequences of from about 9 to about 200 bases having a diol at either or both termini. Preferred diols are polyalkyleneglycols, preferably polyethyleneglycols. Pharmaceutical compositions comprising the compounds and a physiologically acceptable carrier and methods of inhibiting gene expression in mammals comprising administering such compounds are also provided. Methods for inhibiting nuclease cleavage of compounds are also provided.
摘要翻译：公开了抑制基因表达的化合物，组合物和方法。所述化合物包含约9至约200个碱基的寡核苷酸序列，在任一端或两端具有二醇。优选的二醇是聚亚烷基二醇，优选聚乙二醇。还提供了包含化合物和生理上可接受的载体的药物组合物和抑制哺乳动物中基因表达的方法，包括给予这些化合物。还提供了抑制化合物核酸酶切割的方法。

3. 发明授权

US5384242A Diagnostic kit, primer composition and their use for replication or detection of nucleic acids 失效
标题翻译：诊断试剂盒，底漆组合物及其用于复制或检测核酸的用途
公开(公告)号：US5384242A
公开(公告)日：1995-01-24
申请号：US895759
申请日：1992-06-09
申请人： Fred T. Oakes
发明人： Fred T. Oakes
IPC分类号： G01N33/48 , C12Q1/68 , C12Q1/70 , C07H21/04 , C12N15/00 , C12P19/34
CPC分类号： C12Q1/703 , C12Q1/6858
摘要： Amplification, replication or detection of a predetermined target nucleic acid can be carried out using a unique primer composition. This composition comprises and aqueous mixture of a first oligonucleotide primer which is substantially complementary to a first nucleic acid sequence of the target, but which is suspected of having one or more mismatches with the target at or near its 3' end. Also included in the composition is one or more additional primers which are complementary to a nucleic acid sequence of the target. This sequence is either: (i) inclusive of only a portion of the first nucleic acid sequence, (ii) immediately adjacent to the first nucleic acid sequence, or (iii) removed from the first sequence by one or more bases, but which additional primer is capable of forming a primer extension product complementary to the first sequence. These composition components can be supplied as part of a diagnostic test kit which can include other regents if desired.
摘要翻译：可以使用独特的底漆组合物进行预定靶核酸的扩增，复制或检测。该组合物包含第一寡核苷酸引物的水性混合物，该第一寡核苷酸引物基本上与靶标的第一个核酸序列互补，但被怀疑与其3'端或其附近的靶具有一个或多个失配。还包括在组合物中的是一种或多种与目标核酸序列互补的另外的引物。该序列是：（i）仅包括第一核酸序列的一部分，（ii）紧邻第一核酸序列，或（iii）通过一个或多个碱基从第一序列中除去，但另外引物能够形成与第一序列互补的引物延伸产物。这些组合物组分可以作为诊断测试试剂盒的一部分提供，如果需要，其可以包括其他试剂盒。

4. 发明授权

US5245022A Exonuclease resistant terminally substituted oligonucleotides 失效
标题翻译：核酸外切酶末端取代的寡核苷酸
公开(公告)号：US5245022A
公开(公告)日：1993-09-14
申请号：US562180
申请日：1990-08-03
申请人： Alexander L. Weis , Fred T. Oakes , Frederick H. Hausheer , Paul F. Cavanaugh, Jr. , Patricia S. Moskwa
发明人： Alexander L. Weis , Fred T. Oakes , Frederick H. Hausheer , Paul F. Cavanaugh, Jr. , Patricia S. Moskwa
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： Compounds, compositions and methods for inhibiting gene expression are disclosed. The compounds comprise oligonucleotide sequences of from about 9 to about 200 bases having a diol at either or both termini. Preferred diols are polyalkyleneglycols, preferably polyethyleneglycols. Pharmaceutical compositions comprising the compounds and a physiologically acceptable carrier and methods of inhibiting gene expression in mammals comprising administering such compounds are also provided. Methods for inhibiting nuclease cleavage of compounds are also provided.
摘要翻译：公开了抑制基因表达的化合物，组合物和方法。所述化合物包含约9至约200个碱基的寡核苷酸序列，在任一端或两端具有二醇。优选的二醇是聚亚烷基二醇，优选聚乙二醇。还提供了包含化合物和生理上可接受的载体的药物组合物和抑制哺乳动物中基因表达的方法，包括给予这些化合物。还提供了抑制化合物核酸酶切割的方法。

5. 发明授权

US5055414A Phenalenimine Fluorescent dyes and their use in analytical methods 失效
标题翻译：。INE。。。。。。。。。。。。。。。。。。。。。。。。
公开(公告)号：US5055414A
公开(公告)日：1991-10-08
申请号：US215478
申请日：1988-07-05
申请人： Bruce E. Babb , Fred T. Oakes
发明人： Bruce E. Babb , Fred T. Oakes
IPC分类号： G01N33/533 , C07C251/20 , C07D213/74 , C07D221/18 , C09B55/00 , C09B57/00 , C09K11/07 , G01N33/58
CPC分类号： C07D213/74 , C07C251/20 , C07D221/18 , C09B55/009 , C09B57/00 , G01N33/582 , C07C2101/14 , C07C2103/28 , Y10S436/80 , Y10S436/805
摘要： Certain phenalenimine fluorescent compounds represented by the structure ##STR1## wherein R' and R" are independently hydrogen, alkyl, cycloalkyl, aryl or a heterocycle, or R' comprises the carbon and heteroatoms which forms a fused ring with the compound nucleus, are useful in biomedical and analytical determinations. These compounds can be used for staining cells, as well as for the determination of various analytes found in human or animal biological fluids. Such determination can be carried out in solution or by using dry analytical elements. The fluorescent compounds can be reacted with quinone nuclei to form reducible compounds which are also useful in analytical methods. In addition, the compounds can be incorporated into what are known as "loadable" latex particles to form detectable labels and biological reagents.

6. 发明授权

US4962029A Covalent oligonucleotide-horseradish peroxidase conjugate 失效
标题翻译：共价寡核苷酸 - 辣根过氧化物酶缀合物
公开(公告)号：US4962029A
公开(公告)日：1990-10-09
申请号：US437311
申请日：1989-11-16
申请人： Corey Levenson , Chu-an Chang , Fred T. Oakes
发明人： Corey Levenson , Chu-an Chang , Fred T. Oakes
IPC分类号： C07H21/00
CPC分类号： C07H21/00 , Y10S435/964
摘要： Oligonucleotides derivatized to incorporate horseradish peroxidase (HRP) are useful in diagnostic methods and provide significant advantage over radioactively labeled oligonucleotide probes. The HRP-derivatized oligonucleotides can be easily and efficiently prepared by reacting a sulfhydryl containing oligonucleotide with a conjugate of a mal-sac-HNSA ester and HRP under mild and easily controlled reaction conditions.
摘要翻译：衍生成掺入辣根过氧化物酶（HRP）的寡核苷酸可用于诊断方法，并且比放射性标记的寡核苷酸探针提供显着的优点。通过在温和且容易控制的反应条件下使含巯基的寡核苷酸与mal-sac-HNSA酯和HRP的缀合物反应，可以容易且有效地制备HRP衍生的寡核苷酸。

7. 发明授权

US5147777A Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use 失效
标题翻译：含羧基聚合物制备的生物活性试剂，分析元素和使用方法
公开(公告)号：US5147777A
公开(公告)日：1992-09-15
申请号：US539774
申请日：1990-06-18
申请人： Richard C. Sutton , Susan J. Danielson , John B. Findlay , Fred T. Oakes , Marsha D. B. Oenick , Ignazio S. Ponticello , Harold C. Warren, III
发明人： Richard C. Sutton , Susan J. Danielson , John B. Findlay , Fred T. Oakes , Marsha D. B. Oenick , Ignazio S. Ponticello , Harold C. Warren, III
IPC分类号： C08F8/00 , C08F246/00 , C12Q1/68 , C12Q1/70 , G01N33/53 , G01N33/543 , G01N33/545
CPC分类号： G01N33/54313 , C12Q1/6876 , C12Q1/701 , C12Q1/703 , G01N33/545 , Y10S436/805 , Y10T428/2991 , Y10T428/2998
摘要： Biologically active reagents are prepared from particles of copolymers having highly reactive carboxy or equivalent groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through highly reactive carboxy groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents.

8. 发明授权

US5132215A Method of making double-stranded DNA sequences 失效
标题翻译：制作双链DNA序列的方法
公开(公告)号：US5132215A
公开(公告)日：1992-07-21
申请号：US244871
申请日：1988-09-15
申请人： Krishna Jayaraman , Brent A. Burdick , Fred T. Oakes
发明人： Krishna Jayaraman , Brent A. Burdick , Fred T. Oakes
IPC分类号： C12P21/00 , C07K14/80 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/10 , C07K14/80 , C12Q1/6806 , C12Q1/686
摘要： A method of synthesizing double-stranded DNA sequences is disclosed. The method comprises the steps of:(a) preparing a first series of oligodeoxyribonucleotide fragments which, when joined in proper sequence, form a DNA coding strand;(b) preparing a second series of oligodeoxyribonucleotide fragments which, when joined in proper sequence, form a DNA strand complementary to the coding strand;(c) compelling hydrogen bonding and ligation in proper sequence between the first and second series of oligodeoxyribonucleotide fragments prepared in steps (a) and (b) in a single reaction to produce the entire double-stranded DNA sequence;(d) treating the double-stranded DNA sequence with one oligonucleotide primer for each strand under hybridizing conditions;(e) polymerizing an extension product of each primer that is complementary to each strand of the double-stranded DNA sequence which is a template for forming the primer extension product;(f) denaturing the product of step (e) to separate the primer extension products from their respective templates to form four separate single-stranded DNA sequences;(g) treating the denatured product of (f) with oligonucleotide primers, such that a primer extension product is synthesizing using each of the single strands produced in step (f) as a template resulting in amplification of the double-stranded DNA sequence; and(h) repeating steps (d), (e), (f) and (g) until the desired quantity of the double-stranded DNA sequence is formed.

9. 发明授权

US5082780A Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures 失效
标题翻译：可用于混合测定和聚合酶链反应步骤中的探针的寡糖 - 酶联合
公开(公告)号：US5082780A
公开(公告)日：1992-01-21
申请号：US406224
申请日：1989-09-12
申请人： Harold C. Warren, III , Fred T. Oakes
发明人： Harold C. Warren, III , Fred T. Oakes
IPC分类号： C12N9/00 , C07H21/00 , C12N15/09 , C12Q1/68
CPC分类号： C07H21/00
摘要： A covalent conjugate of an enzyme, such as peroxidase, glucose oxidase, alkaline phosphatase and beta-galactosidase, and an oligonucleotide is herein disclosed. This conjugate can be used as a probe in hybridization assays and in polymerase chain reaction procedures.

10. 发明授权

US4914210A Oligonucleotide functionalizing reagents 失效
标题翻译：寡核苷酸功能化试剂
公开(公告)号：US4914210A
公开(公告)日：1990-04-03
申请号：US104200
申请日：1987-10-02
申请人： Corey Levenson , Chu-An Chang , Fred T. Oakes
发明人： Corey Levenson , Chu-An Chang , Fred T. Oakes
IPC分类号： C07C43/11 , C07C43/178 , C07C323/12 , C07D209/48 , C07D317/22 , C07F9/24 , C07F9/26 , C07F9/48 , C07F9/553 , C07H21/00 , C07H21/04 , C08G65/335 , C12Q1/68 , G01N33/535
CPC分类号： C07D209/48 , C07C323/12 , C07C43/11 , C07C43/178 , C07D317/22 , C07F9/2408 , C07F9/2441 , C07F9/2458 , C07F9/2487 , C07F9/26 , C07F9/5537 , C07H21/00 , C08G65/3356 , C12Q1/6816 , G01N33/535
摘要： Oligonucleotide functionalizing reagents are disclosed which are useful in introducing sulfhydryl, amino and additional hydroxyl groups into oligonucleotides. The reagents are substantially linear in structure, at one end provided with a phosphoramidite moiety, at an opposing end provided with a sulfhydryl, amino or hydroxyl moiety, the two ends linked through a hydrophilic spacer chain. Methods of using and synthesizing the novel reagents are disclosed as well.
摘要翻译：公开了寡核苷酸官能化试剂，其用于将巯基，氨基和另外的羟基引入寡核苷酸。试剂在结构上基本上是线性的，在一端具有亚磷酰胺部分，在相对端具有巯基，氨基或羟基部分，两个末端通过亲水间隔链连接。还公开了使用和合成新试剂的方法。

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