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1. 发明授权

US07781573B2 Multi layer chromatography of nucleic acids 有权
标题翻译：核酸多层色谱法
公开(公告)号：US07781573B2
公开(公告)日：2010-08-24
申请号：US11888490
申请日：2007-07-31
申请人： Nam Q Ngo , Laurent Jaquinod
发明人： Nam Q Ngo , Laurent Jaquinod
IPC分类号： C07H21/02 , C07H21/04 , C07H1/06
CPC分类号： C12N15/101
摘要： Methods using two to (n) purification columns to separate full length 5′-DMT-on oligonucleotides with size ranging from 40 to 180-mers from short length 5′-DMT-on oligonucleotides. Two of the said methods require using some columns sequentially with the collection and reprocessing of an intermediate fraction and are used for oligonucleotides with length ranging from 70 to 180-mers. A third method is carried out with columns stacked and used in series and is best used to purify oligonucleotides with length ranging from 40 to 80-mers. In the presence of a high ionic strength buffer, the short length DMT-on oligonucleotides bind to the top stacked columns while the less hydrophobic contaminant or DMT-off failures do not bind and/or are being washed off. In a stacked configuration, the full length DMT-on oligonucleotides are retained by the bottom column while in a 15 sequential configuration, full length DMT-on oligonucleotides are collected and reprocessed.
摘要翻译：使用两对（n）纯化柱分离长度为5'-DMT-寡核苷酸的全长5'-DMT-寡核苷酸的大小从40至180毫升的方法。所述方法中的两个需要依次使用一些列进行中间馏分的收集和再处理，并且用于长度范围为70-180的寡核苷酸。第三种方法用柱堆叠并串联使用，最适用于纯化长度范围为40至80毫升的寡核苷酸。在存在高离子强度缓冲液的情况下，短长度DMT-on寡核苷酸结合顶部堆叠柱，而疏水性较小的污染物或DMT脱离失败不结合和/或被洗掉。在堆叠配置中，全长DMT-on寡核苷酸由底部柱保留，而在15个连续配置中，全长DMT-寡核苷酸被收集并再处理。

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