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1. 发明授权

US08173590B2 AXMI-004, a delta-endotoxin gene and methods for its use 有权
公开(公告)号：US08173590B2
公开(公告)日：2012-05-08
申请号：US12032479
申请日：2008-02-15
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01N37/18 , C07K14/375
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.

2. 发明授权

US08101825B2 AXMI-004, a delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-004，一种δ-内毒素基因及其使用方法
公开(公告)号：US08101825B2
公开(公告)日：2012-01-24
申请号：US12032445
申请日：2008-02-15
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：3和5所示的氨基酸序列的核苷酸序列和SEQ ID NO：1,2和4所示的核苷酸序列的分离的核酸分子，以及作为其变体和片段。

3. 发明授权

US07692068B2 AXMI-010, a delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-010，一种δ-内毒素基因及其使用方法
公开(公告)号：US07692068B2
公开(公告)日：2010-04-06
申请号：US11657964
申请日：2007-01-25
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32 , C12N1/21 , C12P21/04
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子及其变体。

4. 发明授权

US07689366B2 Integrated system for high throughput capture of genetic diversity 有权
标题翻译：用于高产量捕获遗传多样性的综合系统
公开(公告)号：US07689366B2
公开(公告)日：2010-03-30
申请号：US10386401
申请日：2003-03-11
申请人： Nicholas B. Duck , Michael G. Koziel , Nadine Carozzi , Brian Carr , Tracy Hargiss
发明人： Nicholas B. Duck , Michael G. Koziel , Nadine Carozzi , Brian Carr , Tracy Hargiss
IPC分类号： G06F19/00 , C40B30/00 , C40B30/02
CPC分类号： G06F19/22 , C12Q1/6888 , C12Q1/689
摘要： Compositions and methods for rapid and highly efficient characterization of genetic diversity in organisms are provided. The methods involve rapid sequencing and characterization of extrachromosomal DNA, particularly plasmids, to identify useful nucleotide sequences. The method involves generating a library of extrachromosomal DNA clones, sequencing a portion of the clones, comparing the sequences against a database of existing DNA sequences, using an algorithm to select novel nucleotide sequences based on the presence or absence of the sequence in a database, and identification of at least one novel nucleotide sequence. The DNA sequence can also be translated in all six frames and the resulting amino acid sequences compared against a database of protein sequences. Organisms of particular interest include, but are not limited to bacteria, fungi, algae, and the like. Compositions comprise a mini-cosmid vector comprising a stuffer fragment and at least one cos site.
摘要翻译：提供了生物体内遗传多样性快速高效表征的组成和方法。该方法涉及染色体外DNA特别是质粒的快速测序和表征，以鉴定有用的核苷酸序列。该方法包括产生染色体外DNA序列文库，测序一部分克隆，比较序列与现有DNA序列的数据库，使用算法根据数据库中序列的存在或不存在来选择新的核苷酸序列，以及鉴定至少一个新的核苷酸序列。 DNA序列也可以在所有六个帧中翻译，并且得到的氨基酸序列与蛋白质序列的数据库进行比较。特别感兴趣的生物体包括但不限于细菌，真菌，藻类等。组合物包含迷你粘粒载体，其包含填充片段和至少一个cos位点。

5. 发明申请

US20090100543A1 AXMI-018, AXMI-020, AND AXMI-021, A FAMILY OF DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 审中-公开
标题翻译： AXMI-018，AXMI-020和AXMI-021，三肽内毒素基因家族及其使用方法
公开(公告)号：US20090100543A1
公开(公告)日：2009-04-16
申请号：US12192904
申请日：2008-08-15
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck
IPC分类号： A01H1/00 , C12N15/31 , C12N15/64 , C12N5/10 , C12N1/21 , A01H5/00 , A01N37/00 , C12P21/00
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8281 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4或6所示氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列，以及其变体和片段。

6. 发明申请

US20090023654A1 AXMI-008, A DELTA-ENDOTOXIN GENE AND METHODS FOR ITS USE 有权
标题翻译： AXMI-008，DELTA-ENDOTOXIN基因及其使用方法
公开(公告)号：US20090023654A1
公开(公告)日：2009-01-22
申请号：US12026427
申请日：2008-02-05
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01N61/00 , C07K14/00 , A01P7/04 , C07K16/00
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3, 5, and 7, and the nucleotide sequences set forth in SEQ ID NO:1, 2, 4, and 6, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,5和7所示氨基酸序列的核苷酸序列，以及SEQ ID NO：和6，以及其变体和片段。

7. 发明授权

US07253343B2 AXMI-003, a delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-003，一种δ-内毒素基因及其使用方法
公开(公告)号：US07253343B2
公开(公告)日：2007-08-07
申请号：US10926819
申请日：2004-08-26
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01N5/00 , A01N5/10 , C12N15/32 , C12N15/82 , C12P21/04
CPC分类号： C07H21/04 , C07K14/325
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体。

8. 发明申请

US20100298211A1 AXMI-001, AXMI-002, AXMI-030, AXMI-035, AND AXMI-045: TOXIN GENES AND METHODS FOR THEIR USE 审中-公开
标题翻译： AXMI-001，AXMI-002，AXMI-030，AXMI-035和AXMI-045：毒素基因及其使用方法
公开(公告)号：US20100298211A1
公开(公告)日：2010-11-25
申请号：US12721595
申请日：2010-03-11
申请人： Nadine Carozzi , Nicholas B. Duck , Michael G. Koziel , Tracy Hargiss , Rebekah Deter , Cheryl L. Peters , Sandra L. Volrath , Daniel J. Tomso
发明人： Nadine Carozzi , Nicholas B. Duck , Michael G. Koziel , Tracy Hargiss , Rebekah Deter , Cheryl L. Peters , Sandra L. Volrath , Daniel J. Tomso
IPC分类号： A01N37/18 , C07K14/325 , A01P7/04
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:6-11, or the nucleotide sequence set forth in SEQ ID NO:1-5, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：6-11所示氨基酸序列的核苷酸序列或SEQ ID NO：1-5所示的核苷酸序列，以及变体和其片段。

9. 发明授权

US07803391B2 AXMI-028 and AXMI-029, a family of novel delta-endotoxin genes and methods for their use 有权
标题翻译： AXMI-028和AXMI-029是一种新型的δ-内毒素基因及其使用方法
公开(公告)号：US07803391B2
公开(公告)日：2010-09-28
申请号：US12166209
申请日：2008-07-01
申请人： Nadine Carozzi , Michael G. Koziel , Tracy Hargiss , Nicholas B. Duck
发明人： Nadine Carozzi , Michael G. Koziel , Tracy Hargiss , Nicholas B. Duck
IPC分类号： A01N25/00 , A61K38/16 , C12P21/02
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,15,17或19所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 3,14,16或18，以及其变体和片段。

10. 发明申请

US20100037348A1 AXMI-010, A DELTA-ENDOTOXIN GENE AND METHODS FOR ITS USE 有权
标题翻译： AXMI-010，DELTA-ENDOTOXIN基因及其使用方法
公开(公告)号：US20100037348A1
公开(公告)日：2010-02-11
申请号：US11657964
申请日：2007-01-25
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： C12N15/82 , C12N15/11 , C12N15/00 , C12N1/21 , C12N5/04 , A01H5/00 , C12P21/02
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子及其变体。

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