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1. 发明申请

US20220396792A1 METHOD FOR PRODUCING NUCLEIC ACID MOLECULE, BIOMATERIAL, AND METHOD FOR PRODUCING BIOMATERIAL 有权
公开(公告)号：US20220396792A1
公开(公告)日：2022-12-15
申请号：US17773461
申请日：2020-10-29
申请人： NEC Solution Innovators, Ltd. , MOSAIC BIOSCIENCES, INC.
发明人： Tomoko FUJITA , Iwao WAGA , Katsunori HORII , Shintaro KATO , Martin STANTON , Andrew B. DALBY
IPC分类号： C12N15/113 , C07K14/705 , C12N15/62
摘要： The present invention provides a method for producing a nucleic acid molecule that can obtain a nucleic acid molecule that binds to a target and does not inhibit a function of the target. The production method for a nucleic acid molecule of the present invention is a method for producing a nucleic acid molecule that binds to a first biological molecule and does not inhibit a function of the first biological molecule, the method including the steps of: (A) bringing a candidate nucleic acid molecule into contact with the first biological molecule to select a nucleic acid molecule that has bound to the first biological molecule as a first selected nucleic acid molecule; and (B) selecting the first selected nucleic acid molecule as an intended nucleic acid molecule.

2. 发明申请

US20160298117A1 PEANUT-BINDING NUCLEIC ACID MOLECULE AND USE THEREOF 有权
标题翻译： PEANUT-BINDIN NUCLEIC ACID MOLECULLE及其用途
公开(公告)号：US20160298117A1
公开(公告)日：2016-10-13
申请号：US15100833
申请日：2014-06-26
申请人： NEC SOLUTION INNOVATORS, LTD.
发明人： Tomoko MURATA , Katsunori HORII , Ikuo SHIRATORI , Jou AKITOMI , Naoto KANEKO , Iwao WAGA
IPC分类号： C12N15/115 , C12Q1/68
CPC分类号： C12N15/115 , C12N2310/16 , C12Q1/6895
摘要： The present invention provides a novel nucleic acid molecule that can be used for detection of peanuts. The peanut-binding nucleic acid molecule of the present invention is characterized in that it binds to a peanut allergen with a dissociation constant of 10 nM or less, and preferably contains a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 15, for example. It is also preferable that, for example, the peanut-binding nucleic acid molecule of the present invention binds with significant specificity to the peanut allergen rather than to a soybean protein.
摘要翻译：本发明提供了可用于检测花生的新型核酸分子。本发明的花生结合核酸分子的特征在于其以10nM以下的解离常数结合花生过敏原，优选含有由SEQ ID NO：1〜15的碱基序列中的任一种组成的多核苷酸，例如。还优选的是，例如，本发明的花生结合核酸分子对花生过敏原而不是大豆蛋白具有显着特异性的结合。

3. 发明申请

US20210187499A1 TARGET ANALYSIS KIT AND TARGET ANALYSIS METHOD 有权
公开(公告)号：US20210187499A1
公开(公告)日：2021-06-24
申请号：US16318951
申请日：2017-03-23
申请人： NEC SOLUTION INNOVATORS LTD.
发明人： Yoshihito YOSHIDA , Katsunori HORII , Iwao WAGA , Jou AKITOMI , Naoto KANEKO
IPC分类号： B01L3/00 , C12Q1/6813 , G01N33/543
摘要： A target analysis kit includes: a sample holder including: a reagent chamber containing a first reagent; a sample holding portion; and a liquid transfer portion; and an analysis container including: a first chamber containing a second reagent; and a second chamber with a labeling substance contained in the first or second reagent to be detected. A combination of the first and second reagents is any of the combinations (1) to (3) and (4): (1) first reagent is the immobilized second binding substance and second reagent is the labeled first binding substance; (2) first reagent is the labeled first binding substance and second reagent is the immobilized second binding substance; (3) first reagent is the immobilized first binding substance and second reagent is the labeled second binding substance; and (4) first reagent is the labeled second binding substance and second reagent is the immobilized first binding substance.

4. 发明申请

US20180187206A1 NOVEL PROTEIN, NOVEL GENE, EXPRESSION VECTOR, TRANSFORMANT, METHOD FOR PRODUCING TRANSFORMANT, AND METHOD FOR SCREENING FOR NOVEL FLUORESCENT PROTEIN 审中-公开
公开(公告)号：US20180187206A1
公开(公告)日：2018-07-05
申请号：US15737517
申请日：2016-06-13
申请人： NEC Solution Innovators, Ltd.
发明人： Ikuo SHIRATORI , Akihisa SHIMIZU , Katsunori HORII , Hiroshi MISHIMA , Iwao WAGA
IPC分类号： C12N15/82 , C07K14/435 , A01H1/04
CPC分类号： C12N15/8212 , A01H1/04 , C07K14/435 , C07K14/43595 , C12N5/10 , C12N15/09
摘要： The present invention provides a novel fluorescent protein.A novel protein includes the following protein (F1): (F1) a protein consisting of an amino acid sequence of SEQ ID NO: 1, wherein in the amino acid sequence of SEQ ID NO: 1, a 52nd amino acid Xaa52 is an arbitrary amino acid, a 133rd amino acid Xaa133 is an arbitrary amino acid, and a 154th amino acid Xaa154 is an arbitrary amino acid.

5. 发明申请

US20160202154A1 METHOD FOR PRODUCING SAMPLE AND METHOD FOR ANALYZING TARGET 审中-公开
标题翻译：生产样本的方法和分析目标的方法
公开(公告)号：US20160202154A1
公开(公告)日：2016-07-14
申请号：US14916942
申请日：2014-06-27
申请人： NEC SOLUTION INNOVATORS, LTD.
发明人： Katsunori HORII , Jou AKITOMI , Naoto KANEKO , Iwao WAGA
IPC分类号： G01N1/30 , G01N33/538 , G01N33/04 , G01N33/53
CPC分类号： G01N1/30 , C12N15/113 , C12N15/115 , C12N2310/127 , C12N2310/16 , C12N2310/3519 , C12Q1/6806 , G01N30/88 , G01N30/96 , G01N33/04 , G01N33/5308 , G01N33/538 , G01N33/566 , G01N2030/8813 , C12Q2527/125
摘要： The present invention is intended to provide a novel sensor for target analysis and a target analysis method using the same.The sensor for target analysis according to the present invention includes a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule includes a first catalytic nucleic acid region (D1), a second catalytic nucleic acid region (D2), and a binding nucleic acid region (Ap) that binds to a target. The single-stranded nucleic acid molecule includes the first catalytic nucleic acid region (D1) at one end of the binding nucleic acid region (Ap) and the second catalytic nucleic acid region (D2) at the other end of the binding nucleic acid (Ap). In the absence of a target, the catalytic function by the first catalytic nucleic acid region (D1) and the second catalytic nucleic acid region (D2) is inhibited. In the presence of a target, the catalytic function is generated owing to formation of a G-quartet of the first catalytic nucleic acid region (D1) and the second catalytic nucleic acid region (D2) due to the contact between the target and the binding nucleic acid region (Ap).
摘要翻译：本发明旨在提供一种用于目标分析的新型传感器和使用其的目标分析方法。根据本发明的用于靶分析的传感器包括单链核酸分子。单链核酸分子包括与靶标结合的第一催化核酸区域（D1），第二催化核酸区域（D2）和结合核酸区域（Ap）。单链核酸分子包括在结合核酸区（Ap）一端的第一催化核酸区（D1）和结合核酸另一端的第二催化核酸区（D2））。在没有靶的情况下，抑制了第一催化核酸区（D1）和第二催化核酸区（D2）的催化功能。在靶的存在下，由于靶和结合物之间的接触，由于形成第一催化核酸区域（D1）和第二催化核酸区域（D2）的G四重奏，产生催化功能核酸区（Ap）。

6. 发明申请

US20160146772A1 FLUORESCENSE SENSOR FOR TARGET ANALYSIS, KIT FOR TARGET ANALYSIS, AND TARGET ANALYSIS METHOD USING SAME 有权
标题翻译：用于目标分析的荧光传感器，目标分析工具箱和使用相同目标的目标分析方法
公开(公告)号：US20160146772A1
公开(公告)日：2016-05-26
申请号：US14906897
申请日：2014-06-27
申请人： NEC SOLUTION INNOVATORS, LTD.
发明人： Naoto KANEKO , Ikuo SHIRATORI , Katsunori HORII , Jou AKITOMI , Iwao WAGA
IPC分类号： G01N33/04 , C12N15/11 , C12N15/115
CPC分类号： G01N33/04 , C12N15/11 , C12N15/115 , C12N2310/127 , C12N2310/151 , C12N2310/16 , C12N2310/3519 , C12Q1/6825 , G01N33/542 , C12Q2521/345 , C12Q2563/107
摘要： The present invention is intended to provide a novel fluorescence sensor for target analysis, a kit for target analysis, and a target analysis method using the same.The fluorescence sensor for target analysis according to the present invention includes a nucleic acid molecule that includes a G-quartet-forming nucleic acid region (D) that forms a G-quartet and a binding nucleic acid region (A) that binds to a target. In the absence of a target, formation of a G-quartet in the G-quartet-forming nucleic acid region (D) is inhibited. In the presence of a target, the target comes into contact with the binding nucleic acid region (A), the G-quartet is formed in the G-quartet-forming nucleic acid region (D) due to the contact, the G-quartet-forming region (D) and porphyrin forms a complex, and the complex generates fluorescence.
摘要翻译：本发明旨在提供一种用于目标分析的新型荧光传感器，用于目标分析的试剂盒和使用该荧光传感器的目标分析方法。根据本发明的用于靶分析的荧光传感器包括核酸分子，其包括形成G-四重态的G-四重峰形成核酸区（D）和与靶标结合的结合核酸区（A）。在不存在目标的情况下，G-四重峰形成核酸区（D）中的G四重奏的形成被抑制。在靶的存在下，目标与结合核酸区域（A）接触，G-四重峰由于接触而在G-四重峰形成核酸区域（D）中形成，G-四重峰形成区域（D）和卟啉形成复合物，并且复合物产生荧光。

7. 发明申请

US20190183060A1 GLOWING PLANT 审中-公开
公开(公告)号：US20190183060A1
公开(公告)日：2019-06-20
申请号：US16330181
申请日：2017-09-06
申请人： NEC Solution Innovators, Ltd.
发明人： Iwao WAGA , Ayako ISHIWATA , Ikuo SHIRATORI , Akihisa SHIMIZU , Hiroshi MISHIMA
IPC分类号： A01G7/06 , C09K11/06
CPC分类号： A01G7/06 , C09K11/06 , C09K2211/14
摘要： The present invention provides a new glowing plant. The glowing plant of the present invention includes a fluorescent protein applied to a surface of the plant; or a fluorescent protein absorbed inside the plant. The plant is preferably a flower.

8. 发明申请

US20190071735A1 TARGET ANALYSIS METHOD AND TARGET ANALYSIS KIT FOR USE IN THE METHOD 审中-公开
公开(公告)号：US20190071735A1
公开(公告)日：2019-03-07
申请号：US16071977
申请日：2017-01-20
申请人： NEC Solution Innovators, Ltd.
发明人： Yoshihito YOSHIDA , Katsunori HORII , Jou AKITOMI , Naoto KANEKO , Akihisa SHIMIZU , Tomoko FUJITA , Iwao WAGA
IPC分类号： C12Q1/6895 , C12Q1/66 , G01N33/02 , G01N33/00 , G01N33/543
摘要： The present invention provides a new target analysis method and a target analysis kit for use in the method. The target analysis method of the present invention includes: causing a specimen, a labeled binding nucleic acid molecule that binds to a target, and a carrier on which a blocking nucleic acid molecule that binds to the labeled binding nucleic acid molecule is immobilized to react; separating a fraction of the carrier and a fraction of components other than the carrier from each other; and detecting a label of the labeled binding nucleic acid molecule in at least one of the fraction of the carrier and the fraction of components other than the carrier to analyze a target in the specimen.

9. 发明申请

US20170335365A1 METHOD FOR DETECTING BACTERIA 审中-公开
公开(公告)号：US20170335365A1
公开(公告)日：2017-11-23
申请号：US15523920
申请日：2015-07-23
申请人： NEC Solution Innovators, Ltd.
发明人： Yoshihito YOSHIDA , Iwao WAGA
IPC分类号： C12Q1/04 , C12Q1/02 , C12Q1/24
CPC分类号： C12Q1/04 , C12M1/34 , C12Q1/02 , C12Q1/045 , C12Q1/24 , G01N33/56911
摘要： The present invention provides a novel method for more simply and rapidly detecting target bacterial cells by a device using a binding molecule capable of binding to the target bacterial cells. In the method, a sample is incubated in a reagent for concentration of bacteria to cause the sample to react with a fluorescently-labeled binding molecule, and a fluorescence polarization degree is then detect to detect the target, and this is performed using a bacterial detection tool. The bacterial detection tool is obtained by attaching a sample collection tool to a main body. The sample collection tool includes a collection section and a connection section that is connected to the main body. The main body includes a reagent storage chamber, a collection section storage chamber, and a connection section that is connected to the sample collection tool. The reagent storage chamber and the collection section storage chamber are separated from each other. The sample collection tool and the main body are connected to each other at the connection section of the sample collection tool and the connection section of the main body after the preparation step and before the incubation step with the collection section being placed inside the collection section storage chamber. The reagent storage chamber and the collection section storage chamber internally communicate with each other after the preparation step to perform the incubation step and the reaction step. h a sample is collected; and a connection section that is connected to the main body, the main body comprises: a reagent storage chamber that contains the reagent and the fluorescently-labeled binding molecule; a collection section storage chamber that contains the collection section of the sample collection tool; and a connection section that is connected to the sample collection tool, the reagent storage chamber and the collection section storage chamber are separated from each other, and the sample collection tool and the main body are connected to each other at the connection section of the sample collection tool and the connection section of the main body with the collection section of the sample collection tool being placed inside the collection section storage chamber of the main body.

10. 发明申请

US20160230212A1 METHOD AND KIT FOR ANALYZING TARGET 审中-公开
标题翻译：用于分析目标的方法和工具包
公开(公告)号：US20160230212A1
公开(公告)日：2016-08-11
申请号：US15023838
申请日：2014-07-23
申请人： NEC Solution Innovators, Ltd.
发明人： Yoshihito YOSHIDA , Katsunori HORII , Iwao WAGA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6804 , C12Q1/6834 , C12Q2521/325 , C12Q2525/30
摘要： The present invention provides a novel method that can analyze a target easily utilizing binding nucleic acid molecules and an analysis kit for use in the method. The analysis method of the present invention includes: a complex formation step of causing a binding nucleic acid molecule that binds to the target and a sample to come into contact with each other to form a complex of the binding nucleic acid molecule and the target in the sample; a nuclease treatment step of releasing a nucleic acid monomer from at least one of a complex fraction and a non-complex fraction by a nuclease treatment; an enzyme treatment step of reacting the released nucleic acid monomer with an enzyme for which the nucleic acid monomer is a substrate; a detection step of detecting the enzyme reaction; and an analysis step of analyzing the target that has formed the complex from the result of detecting the enzyme reaction.
摘要翻译：本发明提供了一种可以容易地利用结合核酸分子对目标进行分析的新方法和用于该方法的分析试剂盒。本发明的分析方法包括：复合物形成步骤，使与靶标结合的结合核酸分子与样品相互接触，形成结合核酸分子与靶的复合物样品; 通过核酸酶处理从复合部分和非复合部分中的至少一种释放核酸单体的核酸酶处理步骤; 使所述释放的核酸单体与所述核酸单体为底物的酶反应的酶处理步骤; 检测酶反应的检测步骤; 以及从检测酶反应的结果分析形成复合物的靶的分析步骤。

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