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1. 发明授权

US08415119B2 Method for increasing expression yield of a protein of interest 失效
标题翻译：增加感兴趣的蛋白质的表达产率的方法
公开(公告)号：US08415119B2
公开(公告)日：2013-04-09
申请号：US12867612
申请日：2009-02-20
申请人： Mogens Trier Hansen , Birthe Marie Ravn , Leonardo De Maria
发明人： Mogens Trier Hansen , Birthe Marie Ravn , Leonardo De Maria
IPC分类号： C12P21/02 , C07K14/00
CPC分类号： C12P21/02 , C07K14/38 , C12N15/80
摘要： The present invention relates to a method of producing a protein of interest comprising: (a) cultivating a mutant cell under conditions conducive for production of the protein wherein the mutant has a reduced or no expression of an endogenous polypeptide shown in SEQ ID NO: 2 compared to a parent host cell grown under the same conditions; and optionally (b) recovering the protein of interest.
摘要翻译：本发明涉及产生感兴趣的蛋白质的方法，包括：（a）在有助于产生蛋白质的条件下培养突变细胞，其中突变体具有SEQ ID NO：2所示的内源性多肽的减少或不表达与在相同条件下生长的亲本宿主细胞相比; 和（b）回收目的蛋白质。

2. 发明申请

US20110003333A1 Method for Increasing Expression Yield of a Protein of Interest 失效
标题翻译：增加感兴趣的蛋白质的表达产率的方法
公开(公告)号：US20110003333A1
公开(公告)日：2011-01-06
申请号：US12867612
申请日：2009-02-20
申请人： Mogens Trier Hansen , Birthe Marie Ravn , Leonardo De Maria
发明人： Mogens Trier Hansen , Birthe Marie Ravn , Leonardo De Maria
IPC分类号： C12P21/02 , C07K14/00 , C07H21/04 , C12N15/63 , C12N1/00 , C12N1/15 , C12N1/19
CPC分类号： C12P21/02 , C07K14/38 , C12N15/80
摘要： The present invention relates to a method of producing a protein of interest comprising: (a) cultivating a mutant cell under conditions conducive for production of the protein wherein the mutant has a reduced or no expression of an endogenous polypeptide shown in SEQ ID NO: 2 compared to a parent host cell grown under the same conditions; and optionally (b) recovering the protein of interest.
摘要翻译：本发明涉及产生感兴趣的蛋白质的方法，包括：（a）在有助于产生蛋白质的条件下培养突变细胞，其中突变体具有SEQ ID NO：2所示的内源性多肽的减少或不表达与在相同条件下生长的亲本宿主细胞相比; 和（b）回收目的蛋白质。

3. 发明申请

US20120295000A1 Polypeptides and Nucleic Acids Encoding Same 审中-公开
标题翻译：编码相同的多肽和核酸
公开(公告)号：US20120295000A1
公开(公告)日：2012-11-22
申请号：US13566499
申请日：2012-08-03
申请人： Peter Rahbek Oestergaard , Lene Nonboe Anderson , Aurelia Séon , Carlos Simönes-Nunes , Anna-Maria Klünter , Leonardo De Maria , Bjorn Eggert Christensen
发明人： Peter Rahbek Oestergaard , Lene Nonboe Anderson , Aurelia Séon , Carlos Simönes-Nunes , Anna-Maria Klünter , Leonardo De Maria , Bjorn Eggert Christensen
IPC分类号： A23K1/16
CPC分类号： C07K14/32 , A23K10/18 , A23K20/174 , A23K20/30
摘要： The present invention relates to isolated polypeptides and isolated nucleic acid sequences encoding the polypeptides, as well as methods for producing and using the polypeptides in animal feed. An example of a polypeptide of the invention is the so-called L12 protein from Bacillus licheniformis ATCC 14580 which improves the Feed Conversion Ratio (FCR) and/or acts as a gut microflora modulator. The invention furthermore relates to the probiotic use of strains of Bacillus which produce proteins related to L12.
摘要翻译：本发明涉及编码多肽的分离的多肽和分离的核酸序列，以及在动物饲料中产生和使用多肽的方法。本发明的多肽的实例是来自地衣芽孢杆菌ATCC 14580的所谓的L12蛋白，其改进饲料转化率（FCR）和/或作为肠道微生物调节剂。本发明还涉及产生与L12相关的蛋白质的芽孢杆菌菌株的益生菌利用。

4. 发明申请

US20100083392A1 HAFNIA PHYTASE VARIANTS 有权
公开(公告)号：US20100083392A1
公开(公告)日：2010-04-01
申请号：US12568312
申请日：2009-09-28
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： A01K67/00 , C12N9/16 , C12P1/00 , C12P7/06 , C07H21/00 , C12N15/63 , C12N1/00 , C12P21/00 , A01H5/00 , A23L1/28 , A23K1/18 , A23K1/16 , C12G1/00 , C12C11/00 , C12C7/00
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

5. 发明授权

US08697063B2 Polypeptides having phytase activity and polynucleotides encoding same 有权
标题翻译：具有植酸酶活性的多肽和编码其的多核苷酸
公开(公告)号：US08697063B2
公开(公告)日：2014-04-15
申请号：US13327831
申请日：2011-12-16
申请人： Carsten Sjoeholm , Soeren Flensted Lassen , Lars Kobberoee Skov , Leonardo De Maria
发明人： Carsten Sjoeholm , Soeren Flensted Lassen , Lars Kobberoee Skov , Leonardo De Maria
IPC分类号： C12N9/16 , C12P7/06 , C07K14/195 , A61K38/16
CPC分类号： C12N9/16 , A23L33/18 , C12N15/8243 , C12P7/06 , Y02E50/17
摘要： The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有植酸酶活性的多肽。这些多肽具有与来自细菌Buttiauxella的三种植酸酶中的任一种具有至少70％同一性的氨基酸序列，并且其在所示位置包含至少一个以下氨基酸：119N，120L和/或121E。这些植酸酶具有改善的比活性。还公开了另外的特定氨基酸取代，其特征和区分本发明的另外的植酸酶具有改善的性质如温度和/或pH稳定性，pH活性曲线，温度活性分布，底物分布，动物饲料体外或体内改善的性能。本发明还涉及编码多肽的分离的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生和使用多肽的方法。

6. 发明申请

US20130017185A1 Thermostable Phytase Variants 审中-公开
标题翻译：热稳定植酸酶变体
公开(公告)号：US20130017185A1
公开(公告)日：2013-01-17
申请号：US13636199
申请日：2011-03-25
申请人： Leonardo De Maria , Lars Kobberoee Skov , Michael Skjoet
发明人： Leonardo De Maria , Lars Kobberoee Skov , Michael Skjoet
IPC分类号： C12N9/16 , C12S3/02 , A61K38/46 , C12P7/14
CPC分类号： C12N9/16 , A23K20/147 , A23K20/189 , A23K50/10 , A23K50/30 , A23K50/75 , A23K50/80 , A23L33/10 , A23L33/15 , A23L33/16 , A23V2002/00 , C12N15/8243 , C12P7/06 , Y02A40/818 , Y02E50/17
摘要： The present invention relates to a phytase which has at least 70% identity to a phytase derived from E. coli and comprises at least one modification as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensibility, and/or an modified glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
摘要翻译：本发明涉及与来自大肠杆菌的植酸酶具有至少70％同一性的植酸酶，并且与该植酸酶相比包含至少一种修饰。这些植酸酶变体具有修饰的，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如，在动物饲料和动物饲料添加剂中。

7. 发明申请

US20110258714A1 PHYTASE VARIANTS 有权
公开(公告)号：US20110258714A1
公开(公告)日：2011-10-20
申请号：US12294526
申请日：2007-03-19
申请人： Leonardo De Maria , Carsten Andersen , Lars Kobberoee Skov , Mikael Blom Soerensen
发明人： Leonardo De Maria , Carsten Andersen , Lars Kobberoee Skov , Mikael Blom Soerensen
IPC分类号： A01K67/00 , C12N15/63 , C12N5/10 , C07H21/00 , A23L1/304 , A01H5/00 , A23K1/16 , A23K1/18 , A23L1/29 , A23L1/302 , C12N9/16 , C12N1/00
CPC分类号： C12N9/16 , A23K20/189 , C12Y301/03
摘要： The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
摘要翻译：本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如，在动物饲料和动物饲料添加剂中。

8. 发明申请

US20110124084A1 PROTEASES 审中-公开
公开(公告)号：US20110124084A1
公开(公告)日：2011-05-26
申请号：US13014974
申请日：2011-01-27
申请人： Peter Rahbek Oestergaard , Leonardo De Maria
发明人： Peter Rahbek Oestergaard , Leonardo De Maria
IPC分类号： C12N9/48 , C07H21/00 , C12N15/85 , C12N5/10 , A23K1/165 , C11D3/386
CPC分类号： C12N9/58 , A01K2217/05 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g., in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
摘要翻译：本发明涉及肽类家族S2A或S1E的新一类丝氨酸蛋白酶，其在铜（Cu 2+）存在下是稳定的，和/或仅在有限程度上被铜抑制。还揭示了与此相关的结构特征。这类蛋白酶包括衍生自Brachysporiella gayana，Nocardiopsis dassonvillei subsp。的蛋白酶。 dassonvillei，Nocardiopsis prasina和Nocardiopsis alba，但不包括来源于Metarhizium anisopliae和Nocardiopsis sp的已知蛋白酶。 NRRL18262。本发明还涉及编码这种蛋白酶的DNA，其在宿主细胞中的表达，包括动物和植物细胞，以及其在动物饲料和洗涤剂中的用途。特别地，本发明涉及动物饲料和动物饲料添加剂，例如预混物，将这些蛋白酶与1-500ppm Cu（进料中浓度）一起掺入。

9. 发明授权

US07906310B2 Proteases 有权
标题翻译：蛋白酶
公开(公告)号：US07906310B2
公开(公告)日：2011-03-15
申请号：US12466470
申请日：2009-05-15
申请人： Peter Rahbek Oestergaard , Leonardo De Maria
发明人： Peter Rahbek Oestergaard , Leonardo De Maria
IPC分类号： C12N9/52 , C12N15/57 , C12N15/74 , C12N15/75 , C12N15/80 , A23K1/165 , C11D3/386
CPC分类号： C12N9/58 , A01K2217/05 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g., in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
摘要翻译：本发明涉及肽类家族S2A或S1E的新一类丝氨酸蛋白酶，其在铜（Cu 2+）存在下是稳定的，和/或仅在有限程度上被铜抑制。还揭示了与此相关的结构特征。这类蛋白酶包括衍生自Brachysporiella gayana，Nocardiopsis dassonvillei subsp。的蛋白酶。 dassonvillei，Nocardiopsis prasina和Nocardiopsis alba，但不包括来源于Metarhizium anisopliae和Nocardiopsis sp的已知蛋白酶。 NRRL18262。本发明还涉及编码这种蛋白酶的DNA，其在宿主细胞中的表达，包括动物和植物细胞，以及其在动物饲料和洗涤剂中的用途。特别地，本发明涉及动物饲料和动物饲料添加剂，例如预混物，将这些蛋白酶与1-500ppm Cu（进料中浓度）一起掺入。

10. 发明授权

US07588926B2 Proteases 有权
标题翻译：蛋白酶
公开(公告)号：US07588926B2
公开(公告)日：2009-09-15
申请号：US10544861
申请日：2004-02-09
申请人： Peter Rahbek Oestergaard , Leonardo De Maria
发明人： Peter Rahbek Oestergaard , Leonardo De Maria
IPC分类号： C12N9/58 , C12N15/57 , C12N15/74 , C12N15/79 , C11D3/386 , A23K1/165
CPC分类号： C12N9/58 , A01K2217/05 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba. The invention also relates to DNA encoding such proteases and the expression thereof in a host cell, animal and plant cells, as well as to the use thereof in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
摘要翻译：本发明涉及肽类家族S2A或S1E的新一类丝氨酸蛋白酶，其在铜（Cu 2+）存在下是稳定的，和/或仅在有限程度上被铜抑制。还揭示了与此相关的结构特征。这类蛋白酶包括衍生自Brachysporiella gayana，Nocardiopsis dassonvillei subsp。的蛋白酶。 dassonvillei，Nocardiopsis prasina和Nocardiopsis alba。本发明还涉及编码这种蛋白酶的DNA及其在宿主细胞，动物和植物细胞中的表达，以及其在动物饲料和洗涤剂中的用途。特别地，本发明涉及动物饲料和动物饲料添加剂，例如预混物，将这些蛋白酶与1-500ppm Cu（进料中浓度）一起掺入。

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