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1. 发明授权

US06653092B2 Method to determine hydrogen peroxide 失效
标题翻译：测定过氧化氢的方法
公开(公告)号：US06653092B2
公开(公告)日：2003-11-25
申请号：US10003597
申请日：2001-10-30
申请人： Qinghong Han , Li Tang , Mingxu Xu , Yuying Tan , Shigeo Yagi
发明人： Qinghong Han , Li Tang , Mingxu Xu , Yuying Tan , Shigeo Yagi
IPC分类号： C12Q148
CPC分类号： C12Q1/48 , G01N2333/91011
摘要： A method to detect and measure the concentration of hydrogen peroxide comprises adding reagents consisting essentially of peroxidase and a dialkyl phenylenediamine and detecting any color generated by the interaction of hydrogen peroxide with these reagents.
摘要翻译：检测和测量过氧化氢浓度的方法包括加入基本上由过氧化物酶和二烷基苯二胺组成的试剂，并检测由过氧化氢与这些试剂的相互作用产生的任何颜色。

2. 发明授权

US06329162B1 Biological fluid assay methods 有权
标题翻译：生物流体测定方法
公开(公告)号：US06329162B1
公开(公告)日：2001-12-11
申请号：US09550723
申请日：2000-04-17
申请人： Qinghong Han , Li Tang , Mingxu Xu , Yuying Tan , Shigeo Yagi
发明人： Qinghong Han , Li Tang , Mingxu Xu , Yuying Tan , Shigeo Yagi
IPC分类号： C12Q148
CPC分类号： C12Q1/48 , G01N2333/91011
摘要： A method to assess the level of folate in a biological sample comprises: providing said sample with glycine N-methyltransferase (GMT) and with an excess of S-adenosyl methionine (SAM) and of glycine; providing a control which contains no folate with said GMT and excess SAM and glycine in comparable amounts to those provided to the sample; and comparing the concentration of at least one product formed in the sample with the concentrations of said product formed in the control, whereby the difference in levels of said product in the sample as compared to the control is directly proportional to the level of folate in the sample.
摘要翻译：评估生物样品中叶酸水平的方法包括：向所述样品提供甘氨酸N-甲基转移酶（GMT）和过量的S-腺苷甲硫氨酸（SAM）和甘氨酸;提供不含所述叶酸的叶酸的对照 GMT和过量的SAM和甘氨酸与提供给样品的量相当; 并将样品中形成的至少一种产品的浓度与对照中形成的所述产物的浓度进行比较，由此与对照相比，样品中所述产物的水平差异与样品中叶酸水平成正比。

3. 发明授权

US6066467A High specificity homocysteine assays for biological samples 有权
标题翻译：生物样品的高特异性同型半胱氨酸测定
公开(公告)号：US6066467A
公开(公告)日：2000-05-23
申请号：US340991
申请日：1999-06-28
申请人： Mingxu Xu , Yuying Tan , Qinghong Han , Li Tang
发明人： Mingxu Xu , Yuying Tan , Qinghong Han , Li Tang
IPC分类号： C12Q1/527 , G01N33/68 , C12Q1/37 , C12Q1/00
CPC分类号： C12Q1/527 , G01N33/6815 , Y10S435/975
摘要： Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The novel methods of the invention involve use of particular homocysteinase enzymes that permit the determination of homocysteine concentrations in biological samples without interference from the concentrations of cysteine and/or of methionine that are routinely present in such samples. There is also provided a diagnostic kit for use in determining the amount of homocysteine in a biological sample comprising (a) a homocysteinase having the aforementioned characteristics, and (b) at least one reagent capable of being used to determine the amount of product formed in the homocysteinase reaction. In a further aspect, the homocysteinase is provided as a chimeric molecule that comprises amino acid subsequences derived from, or patterned on, more than one homocysteinase, and which is typically produced from a chimeric polynucleotide that encodes therefor. Additional enhancements in homocysteine assay methodology include use of the enzyme .gamma.-glutamylcysteine synthetase to further limit any interference from cysteine present in the biological samples.
摘要翻译：描述了确定生物液体中同型半胱氨酸浓度的新型酶法。在本发明的典型实施方案中，生物流体样品来自患者，本发明的方法可用于评估心血管疾病的风险。本发明的新方法涉及使用特异性同型半胱氨酸酶，其允许在生物样品中测定同型半胱氨酸浓度，而不受这些样品中常规存在的半胱氨酸和/或甲硫氨酸的浓度的干扰。还提供了用于确定生物样品中同型半胱氨酸量的诊断试剂盒，其包含（a）具有上述特征的同型半胱氨酸酶，和（b）至少一种能够用于测定形成的产物量的试剂同型半胱氨酸酶反应。在另一方面，提供同型半胱氨酸酶作为嵌合分子，其包含衍生自或多于一种同型半胱氨酸酶的图案化的氨基酸亚序列，并且其通常由其编码的嵌合多核苷酸产生。同型半胱氨酸测定方法的其它改进包括使用γ-谷氨酰半胱氨酸合成酶来进一步限制存在于生物样品中的半胱氨酸的任何干扰。

4. 发明授权

US06927038B2 Total cysteine assay 失效
标题翻译：总半胱氨酸测定
公开(公告)号：US06927038B2
公开(公告)日：2005-08-09
申请号：US10301531
申请日：2002-11-20
申请人： Qinghong Han , Mingxu Xu , Li Tang , Yuying Tan
发明人： Qinghong Han , Mingxu Xu , Li Tang , Yuying Tan
IPC分类号： C12Q1/527 , C12Q1/34 , C12Q1/48 , G01N33/68 , C12Q3/00 , G01N33/53
CPC分类号： C12Q1/527 , C12Q1/34 , C12Q1/48 , G01N33/6815 , Y10S435/975 , Y10T436/182
摘要： A method to determine a total cysteine in biological fluids utilizes similarly treated portions of the fluid with a homocysteinase and a non-specific desulfurase.
摘要翻译：确定生物流体中的总半胱氨酸的方法利用具有同型半胱氨酸酶和非特异性脱硫酶的类似处理的流体部分。

5. 发明申请

US20050031606A1 Lyase treatment for P. carinii 失效
标题翻译：裂殖酵母处理卡氏菌
公开(公告)号：US20050031606A1
公开(公告)日：2005-02-10
申请号：US10873583
申请日：2004-06-22
申请人： Yuying Tan , Zhijian Yang , Xinghua Sun , Shukuan Li , Qinghong Han , Mingxu Xu
发明人： Yuying Tan , Zhijian Yang , Xinghua Sun , Shukuan Li , Qinghong Han , Mingxu Xu
IPC分类号： A61K20060101 , A61K31/655 , A61K38/00 , A61K38/44 , A61K39/00
CPC分类号： A61K38/51 , A61K31/135 , A61K31/505 , A61K31/635 , A61K45/06 , A61K47/60 , A61K2300/00
摘要： Infection by P. carinii can be treated by administering methioninase optionally in combination with additional therapeutic agents, such as antibiotics.
摘要翻译：可以通过将蛋氨酸酶任选地与另外的治疗剂如抗生素组合来治疗卡氏肺囊虫感染。

6. 发明授权

US5690929A Use of methioninase and chemotherapy agents in chemotherapy 失效
标题翻译：化疗中使用蛋氨酸酶和化疗药物
公开(公告)号：US5690929A
公开(公告)日：1997-11-25
申请号：US424300
申请日：1995-04-24
申请人： Valeryi Lishko , Yuying Tan , Qinghong Han , Mingxu Xu , Huiyan Guo
发明人： Valeryi Lishko , Yuying Tan , Qinghong Han , Mingxu Xu , Huiyan Guo
IPC分类号： C12N15/09 , A61K31/00 , A61K31/337 , A61K31/475 , A61K38/00 , A61K38/46 , A61K38/51 , A61K45/00 , A61K47/48 , A61K48/00 , A61P35/00 , C12N5/10 , C12N9/88 , C12P21/00
CPC分类号： A61K47/48215 , A61K31/337 , A61K38/51 , A61K48/00 , C12N9/88
摘要： The present invention describes methods for using methioninase as an antitumor reagent in anti-methionine chemotherapy. Specifically, methioninase is used to slow or stop cell division which can be enhanced with competitive inhibitors of methionine. In addition, methioninase can be used in combination with chemotherapeutic agents to increase the therapeutic effectiveness of the agent by inducing cell cycle synchronization.
摘要翻译： PCT No.PCT / US93 / 11311 Sec。 371日期1995年04月24日 102（e）日期1995年4月24日PCT提交1993年11月19日PCT公布。第WO94 / 11535号公报日期1994年5月26日本发明描述了在抗甲硫氨酸化疗中使用蛋氨酸酶作为抗肿瘤试剂的方法。具体来说，蛋氨酸酶用于减缓或停止细胞分裂，这可以通过竞争性的甲硫氨酸抑制剂来增强。此外，蛋氨酸酶可以与化学治疗剂组合使用，通过诱导细胞周期同步来增加药剂的治疗效果。

7. 发明授权

US07264819B2 Lyase treatment for P. carinii 失效
标题翻译：裂殖酵母处理卡氏菌
公开(公告)号：US07264819B2
公开(公告)日：2007-09-04
申请号：US10873583
申请日：2004-06-22
申请人： Yuying Tan , Zhijian Yang , Xinghua Sun , Shukuan Li , Qinghong Han , Mingxu Xu
发明人： Yuying Tan , Zhijian Yang , Xinghua Sun , Shukuan Li , Qinghong Han , Mingxu Xu
IPC分类号： A61K39/00 , A61K38/51 , A61K39/385 , A61K38/00
CPC分类号： A61K38/51 , A61K31/135 , A61K31/505 , A61K31/635 , A61K45/06 , A61K47/60 , A61K2300/00
摘要： Infection by P. carinii can be treated by administering methioninase optionally in combination with additional therapeutic agents, such as antibiotics.
摘要翻译：可以通过将蛋氨酸酶任选地与另外的治疗剂如抗生素组合来治疗卡氏肺囊虫感染。

8. 发明授权

US06426194B1 Homogeneous enzymatic assay for vitamin B6 and improvements in H2S detection 有权
标题翻译：维生素B6的均匀酶分析和H2S检测的改进
公开(公告)号：US06426194B1
公开(公告)日：2002-07-30
申请号：US09495889
申请日：2000-02-01
申请人： Mingxu Xu , Qinghong Han , Yuying Tan
发明人： Mingxu Xu , Qinghong Han , Yuying Tan
IPC分类号： C12Q137
CPC分类号： C12Q1/527 , Y10S435/968 , Y10S435/975
摘要： Enzymatic methods to determine the concentration of pyridoxal 5′-phosphate (PLP) in biological fluids are described. The methods of the invention are useful to assess risk for cardiovascular disease. The assay can be a homogeneous assay using the ability of PLP to function as a co-enzyme for homocysteinase and related enzymes and measuring the products of the reaction preferably spectrophotometrically. The invention also includes improvements in sensitivity of assays for measuring hydrogen sulfide production by measuring fluorescence as opposed to absorbance of the oxidized product of H2S with N,N-dialkyl p-phenylene diamine.
摘要翻译：描述了确定生物液体中吡哆醛5'-磷酸（PLP）浓度的酶法。本发明的方法可用于评估心血管疾病的风险。该测定可以是使用PLP用作同型半胱氨酸酶和相关酶的辅酶的能力的均一测定法，并且优选地通过分光光度法测量反应产物。本发明还包括通过测量荧光来测量硫化氢产生的测定的灵敏度的改进，而不是H2S与N，N-二烷基对苯二胺的氧化产物的吸光度。

9. 发明授权

US06524571B1 Methioninase gene therapy for tumor treatment 失效
标题翻译：甲硫氨酸酶基因治疗肿瘤治疗
公开(公告)号：US06524571B1
公开(公告)日：2003-02-25
申请号：US09195055
申请日：1998-11-18
申请人： Mingxu Xu , Yuying Tan
发明人： Mingxu Xu , Yuying Tan
IPC分类号： A01N4304
CPC分类号： G02B5/32 , A01K67/0271 , A61K38/51 , A61K48/00
摘要： A depletion method to inhibit tumor growth includes introducing a viral expression system capable of expressing methioninase or a fusion protein containing methioninase into a tumor contained in a vertebrate subject or cells thereof. The fusion protein may contain a fluorescent protein to permit monitoring of the completeness of the depletion method. The fusion protein can be used in vivo as well as in vitro screening protocols which employ the viral expression system. The expression system includes control sequences and means to integrate the nucleotide sequence into the genome of a host cell for expression. The method may also include treating the cells with isolated methioninase and/or with a therapeutic cell. The depletion method can be with other known therapies, such as maintaining the animal having the tumor on a methionine depleted diet.
摘要翻译：抑制肿瘤生长的消耗方法包括将能够表达蛋氨酸酶的病毒表达系统或含有蛋氨酸酶的融合蛋白引入到脊椎动物对象或其细胞中所含的肿瘤中。融合蛋白可以含有荧光蛋白以允许监测耗尽方法的完整性。融合蛋白可以在体内以及使用病毒表达系统的体外筛选方案中使用。表达系统包括将核苷酸序列整合到宿主细胞的基因组中用于表达的控制序列和方法。该方法还可以包括用分离的蛋氨酸酶和/或用治疗细胞处理细胞。耗尽方法可以与其他已知的疗法一起使用，例如将具有肿瘤的动物维持在消耗蛋氨酸的饮食上。

10. 发明申请

US20120316321A1 METHODS FOR IDENTIFYING MARKERS FOR EARLY-STAGE HUMAN CANCER, CANCER PROGRESSION AND RECURRENCE 审中-公开
标题翻译：识别早期人类癌症标志物的方法，癌症进展和复发
公开(公告)号：US20120316321A1
公开(公告)日：2012-12-13
申请号：US13525174
申请日：2012-06-15
申请人： Mingxu XU , Yuying Tan , Levy Kopelovich
发明人： Mingxu XU , Yuying Tan , Levy Kopelovich
IPC分类号： C40B40/02 , C40B30/06 , C40B40/08 , C07K14/00
CPC分类号： C12N15/1051 , C12N15/1086
摘要： A method is described to identify secreted proteins identified with stages of malignancy of cancer. The proteins are initially identified by trapping them with a fluorescent protein containing vector that can insert in any gene. The secreted proteins are initially identified by their fluorescence. Secreted proteins identifying tumors with specific degrees of malignancy are isolated to determine if they can serve as markers of cancer progression.
摘要翻译：描述了一种鉴定鉴定为癌症恶性程度的分泌蛋白的方法。最初通过用含有可插入任何基因的含荧光蛋白的载体捕获蛋白质来鉴定蛋白质。分泌的蛋白质最初通过其荧光来鉴定。分离识别具有特定恶性程度的肿瘤的分泌蛋白，以确定它们是否可以作为癌症进展的标志物。

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