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1. 发明申请

US20060141465A1 Method of immobilizing a polynucleotide on a solid support, solid immobilization support thus obtained, method of detecting target polynucleotide from the solid support and solution for immobilizing the polynucleotide 审中-公开
标题翻译：将多核苷酸固定在固体支持物上的方法，由此获得的固体固定化载体，从固体支持物检测靶多核苷酸的方法和固定多核苷酸的溶液
公开(公告)号：US20060141465A1
公开(公告)日：2006-06-29
申请号：US10536357
申请日：2003-11-28
申请人： Michifumi Tanga , Hiroshi Okamura , Hirofumi Yamano , Mityuyoshi Ohba , Shuuichi Kamei , Teruhisa Ichihara
发明人： Michifumi Tanga , Hiroshi Okamura , Hirofumi Yamano , Mityuyoshi Ohba , Shuuichi Kamei , Teruhisa Ichihara
IPC分类号： C12Q1/68 , B05D3/02
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00387 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , C12Q1/6837 , C40B40/06 , C40B60/14
摘要： It is intended to provide a method of immobilizing a polynucleotide on a solid support by spotting the polynucleotide on the solid support in a uniform spot shape without any irregularity. Namely, a method of immobilizing a polynucleotide on a solid support by spotting a solution containing the polynucleotide together with polyether or glycol on the solid support.
摘要翻译：旨在提供一种将多核苷酸固定在固体支持物上的方法，其通过以均匀斑点形状将固体支持物上的多核苷酸点样而没有任何不规则性。即，通过将含有多核苷酸的溶液与聚醚或二醇一起点样固定在固体支持物上，将多核苷酸固定在固体支持物上的方法。

2. 发明申请

US20060121473A1 Solid support and method of mass spectrometry of multiple substances or composites immobilized on the solid support through desorption/ionization 审中-公开
标题翻译：通过解吸/电离固定在固体支持物上的多种物质或复合物的固体支持和质谱法
公开(公告)号：US20060121473A1
公开(公告)日：2006-06-08
申请号：US10525394
申请日：2003-08-18
申请人： Michifumi Tanga , Shuuichi Kamei , Hiroshi Okamura , Kaoru Yamakawa , Hirofumi Yamano , Mityuyoshi Ohba , Hisashi Hirano
发明人： Michifumi Tanga , Shuuichi Kamei , Hiroshi Okamura , Kaoru Yamakawa , Hirofumi Yamano , Mityuyoshi Ohba , Hisashi Hirano
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： G01N27/44739 , H01J49/0418
摘要： Means for effecting rapid mass spectrometry of a multiplicity of samples; and a method of rapidly analyzing biosubstances such as nucleic acids and proteins. The above means is a solid support provided with a carbon layer onto which substances separated by gel electrophoresis are transferred. The above method is one for mass spectrometry of multiple substances which comprises separating substances of a sample by gel electrophoresis, transferring separated substances of the gel onto the above solid support so as to immobilize the same and subjecting the immobilized substances to desorption/ionization.
摘要翻译：用于快速质谱分析多种样品的方法; 以及快速分析生物体例如核酸和蛋白质的方法。上述方法是提供有碳层的固体支持物，通过凝胶电泳分离的物质被转移到该碳层上。上述方法是用于通过凝胶电泳分离样品的物质的多种物质的质谱法，将凝胶的分离物质转移到上述固体载体上以使其固定并使固定化物质进行解吸/电离。

3. 发明申请

US20060204959A1 Solid support having electrostatic layer and use thereof 有权
公开(公告)号：US20060204959A1
公开(公告)日：2006-09-14
申请号：US10521176
申请日：2003-07-03
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.

4. 发明授权

US08563239B2 Solid support having electrostatic layer and use thereof 有权
标题翻译：具有静电层的固体支持物及其用途
公开(公告)号：US08563239B2
公开(公告)日：2013-10-22
申请号：US12246152
申请日：2008-10-06
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： C12Q1/68 , C12M1/34 , C12M3/00 , C23C16/00 , C07H21/04
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
摘要翻译：旨在提供能够以高比例固定核酸分子并且对核酸分子具有高粘合强度的固体支持物。固体支持物包括底物，并且在其上提供用于静电吸引核酸分子的静电层和能够与核酸分子共价结合的官能团。

5. 发明授权

US08551765B2 Solid support having electrostatic layer and use thereof 有权
公开(公告)号：US08551765B2
公开(公告)日：2013-10-08
申请号：US10521176
申请日：2003-07-03
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： C12M1/38 , C12M3/00 , C12Q1/68 , C23C16/00 , B05D1/36 , C07H21/02 , C07H21/04
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.

6. 发明申请

US20090111113A1 SOLID SUPPORT HAVING ELECTROSTATIC LAYER AND USE THEREOF 审中-公开
标题翻译：具有静电层的固体支持及其使用
公开(公告)号：US20090111113A1
公开(公告)日：2009-04-30
申请号：US12246082
申请日：2008-10-06
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
摘要翻译：旨在提供能够以高比例固定核酸分子并且对核酸分子具有高粘合强度的固体支持物。固体支持物包括底物，并且在其上提供用于静电吸引核酸分子的静电层和能够与核酸分子共价结合的官能团。

7. 发明申请

US20090098301A1 SOLID SUPPORT HAVING ELECTROSTATIC LAYER AND USE THEREOF 有权
公开(公告)号：US20090098301A1
公开(公告)日：2009-04-16
申请号：US12246152
申请日：2008-10-06
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： B05D1/36
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.

8. 发明申请

US20060024741A1 Support for fixing nucleotide and process for producing the same 审中-公开
标题翻译：支持固定核苷酸及其制备方法
公开(公告)号：US20060024741A1
公开(公告)日：2006-02-02
申请号：US11239418
申请日：2005-09-30
申请人： Michifumi Tanga , Hiroshi Okamura , Kenichi Takagi , Kojiro Takahashi
发明人： Michifumi Tanga , Hiroshi Okamura , Kenichi Takagi , Kojiro Takahashi
IPC分类号： C40B40/08 , C40B50/18
CPC分类号： C07H21/00 , C07B2200/11 , C12N15/1006 , C12Q1/6837 , C12Q2521/501 , C12Q2521/301
摘要： A support for fixing a nucleotide that contributes to the efficient clarification of DNA without damaging the terminal parts of the DNA and, therefore, is useful in the fields of biology, biochemistry and the like. This support for fixing a nucleotide is a chemically modified substrate on which oligonucleotide are immobilized characterized in that the oligonucleotide have a restriction enzyme cleavage site. This support is produced by chemically modifying the substrate, immobilizing a single-stranded oligonucleotide thereon, then hybridizing the single-stranded oligonucleotide with another single-stranded oligonucleotide having a base sequence complementary thereto, and ligating an oligonucleotide having a restriction enzyme site at the end thereof.
摘要翻译：用于固定有助于DNA的有效澄清而不损害DNA的末端部分的核苷酸的支持物，因此可用于生物学，生物化学等领域。用于固定核苷酸的这种支持是其上固定有寡核苷酸的化学修饰底物，其特征在于寡核苷酸具有限制酶切割位点。通过化学修饰底物，将单链寡核苷酸固定在其上，然后将单链寡核苷酸与其碱基序列互补的另一单链寡核苷酸杂交，并在末端连接具有限制酶位点的寡核苷酸，其中。

9. 发明授权

US07078172B1 Substrate activation kit and method for detecting DNA and the like using the same 有权
标题翻译：底物活化试剂盒及其检测方法
公开(公告)号：US07078172B1
公开(公告)日：2006-07-18
申请号：US10344166
申请日：2001-06-20
申请人： Hiroshi Okamura , Michifumi Tanga , Kaoru Yamakawa , Mitsuyoshi Ohba , Kenichi Takagi
发明人： Hiroshi Okamura , Michifumi Tanga , Kaoru Yamakawa , Mitsuyoshi Ohba , Kenichi Takagi
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： G01N33/54353 , C12Q1/6837 , G01N33/552 , C12Q2565/518
摘要： It is intended to provide a method whereby DNA can be conveniently and rigidly immobilized on a substrate at such a high density as achieved by the conventional methods and a method of accurately detecting DNA. A substrate activation kit comprising a phosphate buffer (pH6), a solution A (an aqueous solution) of N-hydroxysuccinimide and a solution B (a dioxide solution) of 1-[3-(dimethylamino) propyl]3-ethylcarbodiimide; and a method of detecting DNA characterized by comprising spotting DNA on a substrate having been activated by using the above activation kit and then hybridizing the spotted DNA with fluorescence labeled DNA.
摘要翻译：旨在提供一种方法，其中DNA可以如通过常规方法实现的高密度方便且刚性地固定在基底上，以及精确检测DNA的方法。包含磷酸盐缓冲液（pH6），N-羟基琥珀酰亚胺的溶液A（水溶液）和1- [3-（二甲基氨基）丙基] -3-乙基碳二亚胺的溶液B（二氧化物溶液）的底物活化试剂盒; 以及一种检测DNA的方法，其特征在于包括通过使用上述活化试剂盒在已经活化的底物上点样DNA，然后将斑点DNA与荧光标记的DNA杂交。

10. 发明申请

US20100300527A1 SUBSTRATE FOR COMPOUND SEMICONDUCTOR SOLAR CELL 审中-公开
标题翻译：复合半导体太阳能电池基板
公开(公告)号：US20100300527A1
公开(公告)日：2010-12-02
申请号：US12790213
申请日：2010-05-28
申请人： Hiroshi Okamura , Hirofumi Yamano , Masanobu Matsubara , Shigeyoshi Nishiyama
发明人： Hiroshi Okamura , Hirofumi Yamano , Masanobu Matsubara , Shigeyoshi Nishiyama
IPC分类号： H01L31/0216
CPC分类号： H01L31/0392 , H01L31/03928 , Y02E10/541
摘要： [Problem] A substrate for a compound semiconductor solar cell which maintains excellent elasticity even after a high temperature process at the time of forming a thin film is provided.[Means for Resolution] The substrate for a compound semiconductor solar cell is formed of a steel plate, and a Cr layer having a coating film quantity of 300 to 8000 mg/m2 is formed on a surface of the substrate on a side to which a solar cell layer is laminated. The Cr layer having a coating film quantity of 500 to 3000 mg/m2 may be formed on the surface of the substrate on a side to which the solar cell layer is laminated, and a film forming temperature of the solar cell layer is less than 550° C. The Cr layer having a coating film quantity of 2000 to 8000 mg/m2 may be formed on the surface of the substrate on a side to which the solar cell layer is laminated, and a film forming temperature of the solar cell layer is more than 800° C. The Cr layer having a coating film quantity of 2000 to 5000 mg/m2 may be formed on the surface of the substrate on a side to which the solar cell layer is laminated, and a film forming temperature of the solar cell layer is not less than 550° C. and not more than 800° C. A quantity of Mn component in a steel strip is not more than 2 wt % . A quantity of Fe component in the steel strip is not more than 98 wt %.
摘要翻译： [问题]提供了一种化合物半导体太阳能电池的基板，其即使在形成薄膜时的高温处理之后也保持优异的弹性。 [解决方法]化合物半导体太阳能电池用基板由钢板形成，并且在基板的表面上形成具有300〜8000mg / m 2的涂膜量的Cr层，层叠太阳能电池层。可以在层叠有太阳能电池层的一侧的基板的表面上形成涂膜量为500〜3000mg / m 2的Cr层，并且太阳能电池层的成膜温度小于550 可以在层叠太阳能电池层的基板的表面上形成涂膜量为2000〜8000mg / m 2的Cr层，太阳能电池层的成膜温度为超过800℃。可以在层叠太阳能电池层的一侧的基板的表面上形成涂膜量为2000〜5000mg / m 2的Cr层，并且形成太阳能电池层的成膜温度电池层不低于550℃并且不超过800℃。钢带中的Mn组分的量不大于2重量％。钢带中的Fe成分量不大于98重量％。

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