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1. 发明申请

US20060204959A1 Solid support having electrostatic layer and use thereof 有权
公开(公告)号：US20060204959A1
公开(公告)日：2006-09-14
申请号：US10521176
申请日：2003-07-03
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.

2. 发明授权

US08563239B2 Solid support having electrostatic layer and use thereof 有权
标题翻译：具有静电层的固体支持物及其用途
公开(公告)号：US08563239B2
公开(公告)日：2013-10-22
申请号：US12246152
申请日：2008-10-06
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： C12Q1/68 , C12M1/34 , C12M3/00 , C23C16/00 , C07H21/04
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
摘要翻译：旨在提供能够以高比例固定核酸分子并且对核酸分子具有高粘合强度的固体支持物。固体支持物包括底物，并且在其上提供用于静电吸引核酸分子的静电层和能够与核酸分子共价结合的官能团。

3. 发明授权

US08551765B2 Solid support having electrostatic layer and use thereof 有权
公开(公告)号：US08551765B2
公开(公告)日：2013-10-08
申请号：US10521176
申请日：2003-07-03
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： C12M1/38 , C12M3/00 , C12Q1/68 , C23C16/00 , B05D1/36 , C07H21/02 , C07H21/04
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.

4. 发明申请

US20090111113A1 SOLID SUPPORT HAVING ELECTROSTATIC LAYER AND USE THEREOF 审中-公开
标题翻译：具有静电层的固体支持及其使用
公开(公告)号：US20090111113A1
公开(公告)日：2009-04-30
申请号：US12246082
申请日：2008-10-06
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
摘要翻译：旨在提供能够以高比例固定核酸分子并且对核酸分子具有高粘合强度的固体支持物。固体支持物包括底物，并且在其上提供用于静电吸引核酸分子的静电层和能够与核酸分子共价结合的官能团。

5. 发明申请

US20090098301A1 SOLID SUPPORT HAVING ELECTROSTATIC LAYER AND USE THEREOF 有权
公开(公告)号：US20090098301A1
公开(公告)日：2009-04-16
申请号：US12246152
申请日：2008-10-06
申请人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
发明人： Hiroshi Okamura , Michifumi Tanga , Hirofumi Yamano , Mitsuyoshi Ohba
IPC分类号： B05D1/36
CPC分类号： C07H21/04 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00722 , C12N15/1096 , C12Q1/6834 , C12Q1/686 , C40B40/06
摘要： It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.

6. 发明申请

US20060141465A1 Method of immobilizing a polynucleotide on a solid support, solid immobilization support thus obtained, method of detecting target polynucleotide from the solid support and solution for immobilizing the polynucleotide 审中-公开
标题翻译：将多核苷酸固定在固体支持物上的方法，由此获得的固体固定化载体，从固体支持物检测靶多核苷酸的方法和固定多核苷酸的溶液
公开(公告)号：US20060141465A1
公开(公告)日：2006-06-29
申请号：US10536357
申请日：2003-11-28
申请人： Michifumi Tanga , Hiroshi Okamura , Hirofumi Yamano , Mityuyoshi Ohba , Shuuichi Kamei , Teruhisa Ichihara
发明人： Michifumi Tanga , Hiroshi Okamura , Hirofumi Yamano , Mityuyoshi Ohba , Shuuichi Kamei , Teruhisa Ichihara
IPC分类号： C12Q1/68 , B05D3/02
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00387 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , C12Q1/6837 , C40B40/06 , C40B60/14
摘要： It is intended to provide a method of immobilizing a polynucleotide on a solid support by spotting the polynucleotide on the solid support in a uniform spot shape without any irregularity. Namely, a method of immobilizing a polynucleotide on a solid support by spotting a solution containing the polynucleotide together with polyether or glycol on the solid support.
摘要翻译：旨在提供一种将多核苷酸固定在固体支持物上的方法，其通过以均匀斑点形状将固体支持物上的多核苷酸点样而没有任何不规则性。即，通过将含有多核苷酸的溶液与聚醚或二醇一起点样固定在固体支持物上，将多核苷酸固定在固体支持物上的方法。

7. 发明申请

US20060121473A1 Solid support and method of mass spectrometry of multiple substances or composites immobilized on the solid support through desorption/ionization 审中-公开
标题翻译：通过解吸/电离固定在固体支持物上的多种物质或复合物的固体支持和质谱法
公开(公告)号：US20060121473A1
公开(公告)日：2006-06-08
申请号：US10525394
申请日：2003-08-18
申请人： Michifumi Tanga , Shuuichi Kamei , Hiroshi Okamura , Kaoru Yamakawa , Hirofumi Yamano , Mityuyoshi Ohba , Hisashi Hirano
发明人： Michifumi Tanga , Shuuichi Kamei , Hiroshi Okamura , Kaoru Yamakawa , Hirofumi Yamano , Mityuyoshi Ohba , Hisashi Hirano
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： G01N27/44739 , H01J49/0418
摘要： Means for effecting rapid mass spectrometry of a multiplicity of samples; and a method of rapidly analyzing biosubstances such as nucleic acids and proteins. The above means is a solid support provided with a carbon layer onto which substances separated by gel electrophoresis are transferred. The above method is one for mass spectrometry of multiple substances which comprises separating substances of a sample by gel electrophoresis, transferring separated substances of the gel onto the above solid support so as to immobilize the same and subjecting the immobilized substances to desorption/ionization.
摘要翻译：用于快速质谱分析多种样品的方法; 以及快速分析生物体例如核酸和蛋白质的方法。上述方法是提供有碳层的固体支持物，通过凝胶电泳分离的物质被转移到该碳层上。上述方法是用于通过凝胶电泳分离样品的物质的多种物质的质谱法，将凝胶的分离物质转移到上述固体载体上以使其固定并使固定化物质进行解吸/电离。

8. 发明申请

US20120088690A1 METHOD FOR DETECTING A POSITION OF PROBE ON MICROARRAY 有权
标题翻译：检测微波探针位置的方法
公开(公告)号：US20120088690A1
公开(公告)日：2012-04-12
申请号：US13378202
申请日：2010-06-10
申请人： Koichi Hirayama , Hirofumi Yamano , Michifumi Tanga
发明人： Koichi Hirayama , Hirofumi Yamano , Michifumi Tanga
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： C12Q1/6813 , G01N21/6428 , G01N21/6452
摘要： A method for detecting hybridization between a probe polynucleotide and a target polynucleotide on a microarray is presented. The method may be used to determine the accuracy of hybridization and possible causes of inaccuracy, such as insufficient washing or deterioration of the microarray.
摘要翻译：提出了一种用于检测微阵列上的探针多核苷酸和靶多核苷酸之间杂交的方法。该方法可以用于确定杂交的准确性和可能的不精确的原因，例如洗涤不足或微阵列的劣化。

9. 发明授权

US08778667B2 Method and unit for detection of interactions of biologically relevant molecules 有权
标题翻译：用于检测生物相关分子相互作用的方法和单元
公开(公告)号：US08778667B2
公开(公告)日：2014-07-15
申请号：US12994990
申请日：2009-05-26
申请人： Hirofumi Yamano , Shuuichi Kamei , Michifumi Tanga
发明人： Hirofumi Yamano , Shuuichi Kamei , Michifumi Tanga
IPC分类号： C12M1/34
CPC分类号： B01L3/50825 , B01L2200/142 , B01L2300/042 , B01L2300/046 , B01L2300/0636 , G01N33/54386
摘要： The present invention relates to a unit to be used for detection of interactions of biologically relevant molecules using a carrier on which the biologically relevant molecules are immobilized, comprising: a hollow holder having an open part at one end with the other end being closed; and a carrier-supporting member that can be inserted into the hollow holder, on which is mounted a carrier upon which biologically relevant molecules are immobilized, wherein: while the carrier-supporting member is being inserted into the hollow holder, a rear-end portion of the carrier-supporting member is engaged with an edge of the open part of the hollow holder, so that the hollow holder is sealed and the positions of the carrier-supporting member and the hollow holder are determined; and the area on the left and the area on the right of the axial center, which are defined by the inner side of the hollow holder and the external side of the carrier-supporting member on which the carrier has been mounted, are approximately the same within the region from the carrier-mounting part to the apical part of the carrier-supporting member, as in a section cut along a plane including the axial center of the hollow holder in a positioned state.
摘要翻译：本发明涉及一种用于检测生物相关分子的相互作用的单元，所述分子使用其上固定有生物相关分子的载体，包括：中空保持器，其一端具有开放部分，另一端封闭; 以及载体支撑构件，其能够插入到所述中空保持器中，所述载体支撑构件安装在固定有生物相关分子的载体上，其中：当所述载体支撑构件插入所述中空保持器中时，后端部载体支撑构件与中空保持器的开口部分的边缘接合，使得中空保持器被密封，并且确定载体支撑构件和中空保持器的位置; 并且由中空保持器的内侧和其上安装有载体的载体支撑构件的外侧限定的左轴和中心的左侧区域和右侧的区域大致相同在从载体安装部到载体支撑构件的顶部的区域内，如在包括处于定位状态的中空保持器的轴心的平面的截面中。

10. 发明授权

US08497068B2 Method for detecting a position of probe on microarray 有权
标题翻译：用于检测微阵列上探针位置的方法
公开(公告)号：US08497068B2
公开(公告)日：2013-07-30
申请号：US13378202
申请日：2010-06-10
申请人： Koichi Hirayama , Hirofumi Yamano , Michifumi Tanga
发明人： Koichi Hirayama , Hirofumi Yamano , Michifumi Tanga
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , G01N21/6428 , G01N21/6452
摘要： A method for detecting hybridization between a probe polynucleotide and a target polynucleotide on a microarray is presented. The method may be used to determine the accuracy of hybridization and possible causes of inaccuracy, such as insufficient washing or deterioration of the microarray.
摘要翻译：提出了一种用于检测微阵列上的探针多核苷酸和靶多核苷酸之间杂交的方法。该方法可以用于确定杂交的准确性和可能的不精确的原因，例如洗涤不足或微阵列的劣化。

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