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    • 7. 发明授权
    • Promoters for expressing genes in a fungal cell
    • 在真菌细胞中表达基因的启动子
    • US06361973B1
    • 2002-03-26
    • US09534407
    • 2000-03-22
    • Randy M. BerkaMichael W. ReyKimberly BrownStephen H. Brown
    • Randy M. BerkaMichael W. ReyKimberly BrownStephen H. Brown
    • C12P2102
    • C12N9/2428C07K14/37C12N15/80
    • The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the nucleic acid sequence, wherein the promoter comprises a sequence selected from the group consisting of nucleotides 1 to 3949 of SEQ ID NO:1, nucleotides 1 to 938 of SEQ ID NO:2, and nucleotides 1 to 3060 of SEQ ID NO:3, and a subsequence thereof; and mutant, hybrid, and tandem promoters thereof; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated promoter sequences and to constructs, vectors, and fungal host cells comprising the promoter sequences operably linked to nucleic acid sequences encoding polypeptides.
    • 本发明涉及生产多肽的方法,其包括:(a)在有助于产生多肽的培养基中培养真菌宿主细胞,其中真菌宿主细胞包含编码多肽的第一核酸序列,其可操作地连接到 第二核酸序列,其包含核酸序列外源的启动子,其中所述启动子包含选自SEQ ID NO:1的核苷酸1至3949,SEQ ID NO:2的核苷酸1至938和核苷酸的核苷酸 SEQ ID NO:3的1〜3060及其子序列; 及其突变体,杂种和串联启动子; 和(b)从培养基中分离多肽。 本发明还涉及分离的启动子序列和构建体,载体和真菌宿主细胞,其包含可操作地连接到编码多肽的核酸序列的启动子序列。