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1. 发明申请

US20100174058A1 FLUORESCENT QUENCHING DETECTING REAGENTS AND METHODS 有权
标题翻译：荧光猝灭检测试剂和方法
公开(公告)号：US20100174058A1
公开(公告)日：2010-07-08
申请号：US12683326
申请日：2010-01-06
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M.J. Vermeulen
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M.J. Vermeulen
IPC分类号： C07H19/16 , C07H21/00
CPC分类号： C07H21/00 , C07B2200/11 , C07C245/08 , C07D519/00 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/04 , C09B29/0813 , C09B69/106 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
摘要翻译：提供了含有两个标记的寡核苷酸探针，可用于杂交测定。探针也可以包含一个小沟结合组。

2. 发明授权

US06727356B1 Fluorescent quenching detection reagents and methods 有权
标题翻译：荧光猝灭检测试剂及方法
公开(公告)号：US06727356B1
公开(公告)日：2004-04-27
申请号：US09457616
申请日：1999-12-08
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy
IPC分类号： C07H1900
CPC分类号： C07D519/00 , C07B2200/11 , C07C245/08 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide-fluorophore-quencher conjugates wherein the fluorophore moiety has emission wavelengths in the range of about 300 to about 800 nm, and or where the quencher includes a substituted 4-(phenyldiazenyl)phenylamine structure provide improved signal to noise ratios and other advantageous characteristics in hybridization and related assays. The oligonucleotide-fluorophore-quencher conjugates can be synthesized by utilizing novel phosphoramidite reagents that incorporate the quencher moiety based on the substituted 4-(phenyldiazenyl)phenylamine structure, and or novel phosphoramidite reagents that incorporate a fluorophore moiety based on the substituted coumarin, substituted 7-hydroxy-3H-phenoxazin-3-one, or substituted 5,10-dihydro-10-[phenyl]pyrido[2,3-d;6,5-d′]dipyrimidine-2,4,6,8-(1H,3H,7H,9H,10H)-tetrone structure. Oligonucleotide-fluorophore-quencher-minor groove binder conjugates including a pyrrolo[4,5-e]indolin-7-yl}carbonyl)pyrrolo[4,5-e]indolin-7-yl]carbonyl}pyrrolo[4,5-e]indoline-7-carboxylate (DPI3) moiety as the minor groove binder and the substituted 4-(phenyldiazenyl)phenylamine moiety as the quencher, were synthesized and have substantially improved hybridization and signal to noise ratio properties.
摘要翻译：寡核苷酸 - 荧光团 - 猝灭剂缀合物，其中荧光团部分具有在约300至约800nm范围内的发射波长，或者猝灭剂包含取代的4-（苯基二氮烯基）苯胺结构的提供改善的信噪比和其它有利特征杂交和相关测定。寡核苷酸 - 荧光团 - 猝灭剂缀合物可以通过利用引入基于取代的4-（苯基二氮烯基）苯基胺结构的猝灭剂部分的新型亚磷酰胺试剂，和/或新型亚磷酰胺试剂合成，所述亚磷酰胺试剂掺入基于取代香豆素的荧光团部分，取代7 - 羟基-3H-吩恶嗪-3-酮或取代的5,10-二氢-10- [苯基]吡啶并[2,3-d; 6,5-d']二嘧啶-2,4,6,8-（ 1H，3H，7H，9H，10H） - 四酮结构。吡咯并[4,5-e]二氢吲哚-7-基}羰基）吡咯并[4,5-e]二氢吲哚-7-基]羰基}吡咯并[4,5- e]二氢吲哚-7-羧酸酯（DPI 3）部分作为次槽粘合剂和取代的4-（苯基二氮烯基）苯胺部分作为猝灭剂，并且具有显着改善的杂交和信噪比性质。

3. 发明授权

US07662942B2 Fluorescent quenching detection reagents and methods 有权
标题翻译：荧光猝灭检测试剂及方法
公开(公告)号：US07662942B2
公开(公告)日：2010-02-16
申请号：US11338866
申请日：2006-01-23
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
IPC分类号： C07H21/00 , C07H21/02 , C12Q1/68
CPC分类号： C07H21/00 , C07B2200/11 , C07C245/08 , C07D519/00 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/04 , C09B29/0813 , C09B69/106 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
摘要翻译：提供了含有两个标记的寡核苷酸探针，可用于杂交测定。探针也可以包含一个小沟结合组。

4. 发明授权

US06699975B2 Fluorescent quenching detection reagents and methods 有权
标题翻译：荧光猝灭检测试剂及方法
公开(公告)号：US06699975B2
公开(公告)日：2004-03-02
申请号：US10093769
申请日：2002-03-07
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy
IPC分类号： C07C24500
CPC分类号： C07D519/00 , C07B2200/11 , C07C245/08 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide-fluorophore-quencher conjugates wherein the fluorophore moiety has emission wavelengths in the range of about 300 to about 800 nm, and or where the quencher includes a substituted 4-(phenyldiazenyl)phenylamine structure provide improved signal to noise ratios and other advantageous characteristics in hybridization and related assays. The oligonucleotide-fluorophore-quencher conjugates can be synthesized by utilizing novel phosphoramidite reagents that incorporate the quencher moiety based on the substituted 4-(phenyidiazenyl)phenylamine structure, and or novel phosphoramidite reagents that incorporate a fluorophore moiety based on the substituted coumarin, substituted 7-hydroxy-3H-phenoxazin-3-one, or substituted 5,10-dihydro-10-[phenyl]pyrido[2,3-d;6,5-d′]dipyrimidine-2,4,6,8-(1H,3H,7H,9H,10H)-tetrone structure. Oligonucleotide-fluorophore-quencher-minor groove binder conjugates including a pyrrolo[4,5-e]indolin-7-yl}carbonyl) pyrrolo[4,5-e]indolin-7-yl]carbonyl}pyrrolo[4,5-e]indoline-7-carboxylate (DPI3) moiety as the minor groove binder and the substituted 4-(phenyldiazenyl)phenylamine moiety as the quencher, were synthesized and have substantially improved hybridization and signal to noise ratio properties.
摘要翻译：寡核苷酸 - 荧光团 - 猝灭剂缀合物，其中荧光团部分具有在约300至约800nm范围内的发射波长，或者其中猝灭剂包括取代的4-（苯基二氮烯基）苯胺结构提供改善的信噪比和其它有利特征杂交和相关测定。寡核苷酸 - 荧光团 - 猝灭剂缀合物可以通过利用基于取代的4-（苯基亚基）苯胺结构的掺入猝灭剂部分的新型亚磷酰胺试剂和/或掺入基于取代香豆素的荧光团部分的新型亚磷酰胺试剂来合成，取代7 - 羟基-3H-吩恶嗪-3-酮或取代的5,10-二氢-10- [苯基]吡啶并[2,3-d; 6,5-d']二嘧啶-2,4,6,8-（ 1H，3H，7H，9H，10H） - 四酮结构。吡咯并[4,5-e]二氢吲哚-7-基}羰基）吡咯并[4,5-e]二氢吲哚-7-基]羰基}吡咯并[4,5- e]二氢吲哚-7-羧酸酯（DPI 3）部分作为次槽粘合剂和取代的4-（苯基二氮烯基）苯胺部分作为猝灭剂，并且具有显着改善的杂交和信噪比性质。

5. 发明授权

US07897736B2 Fluorescent quenching detecting reagents and methods 有权
标题翻译：荧光猝灭检测试剂及方法
公开(公告)号：US07897736B2
公开(公告)日：2011-03-01
申请号：US12683326
申请日：2010-01-06
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
IPC分类号： C07G3/00 , C07H21/00 , C07H21/02 , C07H19/04 , C12Q1/68
CPC分类号： C07H21/00 , C07B2200/11 , C07C245/08 , C07D519/00 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/04 , C09B29/0813 , C09B69/106 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
摘要翻译：提供了含有两个标记的寡核苷酸探针，可用于杂交测定。探针也可以包含一个小沟结合组。

6. 发明授权

US06653473B2 Fluorescent quenching detection reagents and methods 有权
公开(公告)号：US06653473B2
公开(公告)日：2003-11-25
申请号：US10084818
申请日：2002-02-26
申请人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
发明人： Michael W. Reed , Eugeny Alexander Lukhtanov , Alexander A. Gall , Robert O. Dempcy , Nicolaas M. J. Vermeulen
IPC分类号： C07D28816
CPC分类号： C07D519/00 , C07B2200/11 , C07C245/08 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.

7. 发明授权

US5667976A Solid supports for nucleic acid hybridization assays 失效
标题翻译：用于核酸杂交测定的固体支持物
公开(公告)号：US5667976A
公开(公告)日：1997-09-16
申请号：US601419
申请日：1996-02-14
申请人： Jeffrey Van Ness , Charles R. Petrie , John C. Tabone , Nicolaas M.J. Vermeulen , Michael W. Reed
发明人： Jeffrey Van Ness , Charles R. Petrie , John C. Tabone , Nicolaas M.J. Vermeulen , Michael W. Reed
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： C07H21/00 , Y10S436/81
摘要： Compositions and methods for covalently immobilizing an oligonucleotide onto a polymer-coated solid support or similar structure are provided. Specifically, the polymer-coated support, such as a bead, possesses a large number of activatable moieties, preferably primary and secondary amines. An oligonucleotide is activated with a monofunctional or multifunctional reagent, preferably the homotrifunctional reagent cyanuric chloride. The resultant covalently immobilized oligonucleotides on the support serve as nucleic acid probes, and hybridization assays can be conducted wherein specific target nucleic acids are detected in complex biological samples. The beads or similar structures can be employed free in solution, such as in a microtiter well format; in a flow-through format, such as in a column; or in a dipstick. Additionally, dichlorotriazine oligonucleotides and processes for activating oligonucleotides by treatment with cyanuric chloride and derivatives are included in the present invention.
摘要翻译：提供了将寡核苷酸共价固定在聚合物涂覆的固体支持物或类似结构上的组合物和方法。具体来说，聚合物涂覆的载体如珠粒具有大量的可活化部分，优选伯胺和仲胺。寡核苷酸用单官能或多功能试剂，优选同三功能试剂氰尿酰氯活化。所得到的载体上共价固定的寡核苷酸用作核酸探针，并且可以进行杂交测定，其中在复杂生物样品中检测到特异性靶核酸。珠或类似结构可以在溶液中自由使用，例如以微量滴定孔形式存在; 以流通格式，例如列; 或在量油尺中。此外，二氯三嗪寡核苷酸和通过用氰尿酰氯及其衍生物处理来活化寡核苷酸的方法包括在本发明中。

8. 发明申请

US20120322161A1 FLUORESCENT pH DETECTOR SYSTEM AND RELATED METHODS 有权
标题翻译：荧光检测器系统及相关方法
公开(公告)号：US20120322161A1
公开(公告)日：2012-12-20
申请号：US13482687
申请日：2012-05-29
申请人： Michael W. Reed , Steven J. Geelhood , Robert O. Dempcy
发明人： Michael W. Reed , Steven J. Geelhood , Robert O. Dempcy
IPC分类号： G01N21/64
CPC分类号： G01N21/81 , G01N2021/6484 , G01N2021/7786 , G01N2201/0846
摘要： Fluorescent pH detector and methods for measuring pH using the fluorescent pH detector.
摘要翻译：荧光pH检测器和使用荧光pH检测器测量pH的方法。

9. 发明授权

US09040307B2 Fluorescent pH detector system and related methods 有权
公开(公告)号：US09040307B2
公开(公告)日：2015-05-26
申请号：US13482687
申请日：2012-05-29
申请人： Michael W. Reed , Steven J. Geelhood , Robert O. Dempcy
发明人： Michael W. Reed , Steven J. Geelhood , Robert O. Dempcy
IPC分类号： G01N31/16 , G01N21/81 , G01N21/64 , G01N21/77
CPC分类号： G01N21/81 , G01N2021/6484 , G01N2021/7786 , G01N2201/0846
摘要： Fluorescent pH detector and methods for measuring pH using the fluorescent pH detector.

10. 发明申请

US20130130402A1 FLUORESCENT COMPOUNDS, COMPOSITIONS, AND METHODS FOR USING THE COMPOUNDS AND COMPOSITIONS 审中-公开
标题翻译：荧光化合物，组合物和使用该化合物和组合物的方法
公开(公告)号：US20130130402A1
公开(公告)日：2013-05-23
申请号：US13478071
申请日：2012-05-22
申请人： Michael W. Reed , Robert O. Dempcy
发明人： Michael W. Reed , Robert O. Dempcy
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , G01N21/6428 , G01N21/77 , G01N21/80 , G01N33/582 , G01N2021/6421 , G01N2021/6484 , G01N2021/7786 , G01N2201/084
摘要： Low pKa fluorescent compounds, compositions that include the compounds, bioconjugates made from the compounds, and methods for making and using the compounds and bioconjugates.
摘要翻译：低pKa荧光化合物，包括化合物的组合物，由化合物制成的生物缀合物，以及制备和使用化合物和生物缀合物的方法。

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