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1. 发明授权

US09334495B2 Minor groove binder (MGB)-oligonucleotide miRNA antagonists 有权
公开(公告)号：US09334495B2
公开(公告)日：2016-05-10
申请号：US12953098
申请日：2010-11-23
申请人： Anastasia Khvorova , Annaleen Vermeulen , Rob Kaiser , Jon Karpilow , Nicolaas M. J. Vermeulen , Walt Mahoney
发明人： Anastasia Khvorova , Annaleen Vermeulen , Rob Kaiser , Jon Karpilow , Nicolaas M. J. Vermeulen , Walt Mahoney
IPC分类号： C12N15/11 , C07H21/02 , C12Q1/68
CPC分类号： C12N15/111 , C12N2310/113 , C12N2310/321 , C12N2310/351 , C12N2310/3521
摘要： Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.

2. 发明授权

US06951930B2 Hybridization-triggered fluorescent detection of nucleic acids 失效
标题翻译：杂交荧光检测核酸
公开(公告)号：US06951930B2
公开(公告)日：2005-10-04
申请号：US10227001
申请日：2002-08-21
申请人： Robert O. Dempcy , Irina Aleksandrovna Afonina , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Irina Aleksandrovna Afonina , Nicolaas M. J. Vermeulen
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C12Q1/6816 , C12Q2563/107 , C12Q2565/107 , C12Q2563/173
摘要： Compositions and methods for fluorescent detection of nucleic acids are provided. The compositions can be detected by fluorescence when hybridized to a nucleic acid containing a target sequence, but are non-fluorescent in the non-hybridized state. Alternatively, the fluorescence properties of the compositions change in a detectable manner upon hybridization to a nucleic acid containing a target sequence. Methods for synthesis and methods of use of the compositions are also provided.
摘要翻译：提供了核酸荧光检测的组合物和方法。当与含有靶序列的核酸杂交时，可以通过荧光检测组合物，但是在非杂交状态下是非荧光的。或者，当与含有靶序列的核酸杂交时，组合物的荧光特性以可检测的方式变化。还提供了合成方法和组合物的使用方法。

3. 发明授权

US08569516B2 Compounds and methods for fluorescent labeling 有权
标题翻译：用于荧光标记的化合物和方法
公开(公告)号：US08569516B2
公开(公告)日：2013-10-29
申请号：US11357750
申请日：2006-02-17
申请人： Eugeny A. Lukhtanov , Alexei V. Vorobiev , Michael W. Reed , Nicolaas M. J. Vermeulen
发明人： Eugeny A. Lukhtanov , Alexei V. Vorobiev , Michael W. Reed , Nicolaas M. J. Vermeulen
IPC分类号： C07D311/82
CPC分类号： C07D311/78 , C07D493/10 , C07D493/20 , C07D497/10 , C07D497/20 , C07H21/04
摘要： Compounds useful in labeling biological materials are provided along with methods for their use.
摘要翻译：提供了用于标记生物材料的化合物及其使用方法。

4. 发明授权

US07723038B2 Amplification methods 有权
标题翻译：扩增方法
公开(公告)号：US07723038B2
公开(公告)日：2010-05-25
申请号：US11958895
申请日：2007-12-18
申请人： Walt Mahoney , Nicolaas M. J. Vermeulen , Irina Afonina
发明人： Walt Mahoney , Nicolaas M. J. Vermeulen , Irina Afonina
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H19/04
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/6827 , C12Q2565/1015 , C12Q2563/173 , C12Q2561/113 , C12Q2561/101
摘要： Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.
摘要翻译：提供了用于扩增和监测寡核苷酸扩增的方法，其中引物与检测探针的一个或多个碱基重叠。

5. 发明授权

US07601851B2 Compounds and methods for fluorescent labeling 有权
标题翻译：用于荧光标记的化合物和方法
公开(公告)号：US07601851B2
公开(公告)日：2009-10-13
申请号：US11127040
申请日：2005-05-10
申请人： Eugeny A. Lukhtanov , Alexei V. Vorobiev , Michael W. Reed , Nicolaas M. J. Vermeulen
发明人： Eugeny A. Lukhtanov , Alexei V. Vorobiev , Michael W. Reed , Nicolaas M. J. Vermeulen
IPC分类号： C07D311/82 , C07D311/86 , C12Q1/68
CPC分类号： C09B57/14 , C09B11/08 , C09B19/00 , C09B57/02 , G01N33/533
摘要： Dye reagents useful in labeling biological materials are provided along with methods for their use.
摘要翻译：提供用于标记生物材料的染料试剂及其使用方法。

6. 发明授权

US07582739B2 Negatively charged minor groove binders 有权
标题翻译：带负电荷的小槽粘合剂
公开(公告)号：US07582739B2
公开(公告)日：2009-09-01
申请号：US10507267
申请日：2003-03-11
申请人： Eugeny A. Lukhtanov , Nicolaas M. J. Vermeulen , Alexander A. Gall
发明人： Eugeny A. Lukhtanov , Nicolaas M. J. Vermeulen , Alexander A. Gall
IPC分类号： C07H21/04
CPC分类号： C07H21/04 , C07H21/00
摘要： The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
摘要翻译：本发明提供带负电荷的小沟结合化合物，包含其的寡核苷酸缀合物及其使用方法。本发明的带负电荷的小沟结合化合物包含能够在生理条件下电离的酸性部分。特别地，本发明的带负电荷的小沟结合化合物包含优先结合双重，三重或更高链DNA，RNA，PNA或其杂交体的小沟的结合部分。结合部分包含至少一个芳基部分和酸性部分，其共价连接到芳基部分的苯基部分或芳基部分的杂芳基部分的杂原子。

7. 发明授权

US06683173B2 Tm leveling methods 有权
标题翻译：调平方法
公开(公告)号：US06683173B2
公开(公告)日：2004-01-27
申请号：US10032307
申请日：2001-12-21
申请人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
IPC分类号： C07H2104
CPC分类号： C07H21/00 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C12Q1/6832 , C40B40/00 , G06F19/22 , C12Q2525/117
摘要： Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要翻译：提供了修饰的寡核苷酸，其含有选自未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的碱，并且任选具有连接的小沟结合剂和报道基团。

8. 发明授权

US06660845B1 Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof 有权
标题翻译：包含核苷酸类似物的非聚集性非猝灭性低聚物; 合成和使用方法
公开(公告)号：US06660845B1
公开(公告)日：2003-12-09
申请号：US09447936
申请日：1999-11-23
申请人： Alexander A. Gall , Igor V. Kutyavin , Nicolaas M. J. Vermeulen , Robert O. Dempcy
发明人： Alexander A. Gall , Igor V. Kutyavin , Nicolaas M. J. Vermeulen , Robert O. Dempcy
IPC分类号： C07H2100
CPC分类号： C07D487/04 , A61K31/519 , C07H21/00 , C07K5/06026 , C07K5/06139 , C07K14/003 , C12Q1/6818 , C12Q1/6832 , C12Q2525/107 , C12Q2525/101
摘要： The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced fluorescence quenching are obtained when the oligomers are used in various methods involving hybridization. Methods of synthesis, as well as novel synthetic precursors, are also provided.
摘要翻译：本发明提供使用DNA，RNA，PNA和嵌合寡聚物改进杂交分析的组合物和方法，其中一个或多个嘌呤碱基被吡唑并[5,4-d]嘧啶或7-脱氮嘌呤嘌呤类似物取代。当寡聚物以涉及杂交的各种方法使用时，获得了减少的自聚集和减少的荧光猝灭。还提供了合成方法，以及新型合成前体。

9. 发明授权

US5872104A Combinations and methods for reducing antimicrobial resistance 失效
标题翻译：减少抗菌素耐药性的组合和方法
公开(公告)号：US5872104A
公开(公告)日：1999-02-16
申请号：US364246
申请日：1994-12-27
申请人： Nicolaas M. J. Vermeulen , Dennis E. Schwartz
发明人： Nicolaas M. J. Vermeulen , Dennis E. Schwartz
IPC分类号： A61K45/06 , A61K31/70
CPC分类号： A61K45/06 , Y10S977/80 , Y10S977/801
摘要： Disclosed are novel methods, combinations of agents and kits for use in killing, or inhibiting the growth of, microorganisms. Enhanced antimicrobial action is provided by using a methylation inhibitor, as exemplified by using an agent that inhibits methylation or maturation of bacterial RNA in combination with, e.g., a macrolide lincosamide streptogramin B (MLS) antibiotic. The methods and compositions described may be employed to reduce the resistance of susceptible microorganisms to antimicrobial agents and thus to treat animals or patients with infections.
摘要翻译：公开了用于杀死或抑制微生物生长的新方法，试剂和试剂盒的组合。通过使用甲基化抑制剂提供增强的抗微生物作用，例如通过使用抑制细菌RNA的甲基化或成熟的药剂与例如大环内酯氯胺素链霉素B（MLS）抗生素的组合来实施。可以使用所描述的方法和组合来降低敏感微生物对抗微生物剂的抵抗力，从而治疗感染的动物或患者。

10. 发明授权

US5393672A Non toxic compositions and methods useful for the extraction of nucleic acids 失效
标题翻译：用于提取核酸的无毒组合物和方法
公开(公告)号：US5393672A
公开(公告)日：1995-02-28
申请号：US156519
申请日：1993-11-23
申请人： Jeffrey V. Ness , B. Melina Cimler , Rich B. Meyer, Jr. , Nicolaas M. J. Vermeulen
发明人： Jeffrey V. Ness , B. Melina Cimler , Rich B. Meyer, Jr. , Nicolaas M. J. Vermeulen
IPC分类号： C12N15/10 , C12Q1/68 , G01N33/50
CPC分类号： C12Q1/6806 , C12N15/1003 , Y10T436/143333 , Y10T436/25125 , Y10T436/25375
摘要： This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
摘要翻译：本发明涉及安全有效的提取核酸的方法。特别地，描述了从含有核酸和其他生物化合物的生物混合物的样品中分离核酸的方法，其中样品与含有至少一种有机化合物如苄醇或苄醇衍生物的提取溶液组合形成水相和非水相。从水相中分离核酸。优选地，所得到的组合溶液还含有如下定义的膨润土。通常，样品首先在萃取前与裂解剂组合。优选的裂解剂是离液盐，例如盐酸胍和异硫氰酸胍。

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