专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20090036323A1 STRATEGIES FOR HIGH THROUGHPUT IDENTIFICATION AND DETECTION OF POLYMORPHISMS 有权
标题翻译：用于高通量识别和多态性检测的策略
公开(公告)号：US20090036323A1
公开(公告)日：2009-02-05
申请号：US11993945
申请日：2006-06-23
申请人： Michael Josephus Theresia van Eijk , Henricus Johannes Adam van der Poel
发明人： Michael Josephus Theresia van Eijk , Henricus Johannes Adam van der Poel
IPC分类号： C40B30/04
CPC分类号： G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
摘要： The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
摘要翻译：本发明涉及通过在两个或多个样品上进行复杂性降低以产生两个或更多个文库，测序至少部分文库，对齐所鉴定的序列并确定任何推定的单核苷酸的方法，用于高通量鉴定单核苷酸多态性的方法多态性，确认任何推定的单核苷酸多态性，产生用于确认的单核苷酸多态性的检测探针，将测试样品命名为相同的复杂性降低以提供测试文库并筛选测试文库以存在或不存在单核苷酸多态性检测探头。

2. 发明授权

US08685889B2 Strategies for high throughput identification and detection of polymorphisms 有权
标题翻译：高通量鉴定和多态性检测策略
公开(公告)号：US08685889B2
公开(公告)日：2014-04-01
申请号：US13449611
申请日：2012-04-18
申请人： Michael Josephus Theresia van Eijk , Henricus Johannes Adam van der Poel
发明人： Michael Josephus Theresia van Eijk , Henricus Johannes Adam van der Poel
IPC分类号： C40B20/00
CPC分类号： G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
摘要： The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
摘要翻译：本发明涉及通过在两个或多个样品上进行复杂性降低以产生两个或更多个文库，测序至少部分文库，对齐所鉴定的序列并确定任何推定的单核苷酸的方法，用于高通量鉴定单核苷酸多态性的方法多态性，确认任何推定的单核苷酸多态性，产生用于确认的单核苷酸多态性的检测探针，将测试样品命名为相同的复杂性降低以提供测试文库并筛选测试文库以存在或不存在单核苷酸多态性检测探头。

3. 发明申请

US20090208943A1 Method for the High Throughput Screening of Transposon Tagging Populations and Massive Parallel Sequence Identification of Insertion Sites 失效
标题翻译：用于高吞吐量筛选转座子标记群体的方法和插入位点的大规模并行序列识别
公开(公告)号：US20090208943A1
公开(公告)日：2009-08-20
申请号：US12093574
申请日：2006-11-08
申请人： Michael Josephus Theresia van Eijk , Antonius Gerardus Marie Gerats , Adrianus Johannes van Tunen , Michiel Marcel Albert Vandenbussche
发明人： Michael Josephus Theresia van Eijk , Antonius Gerardus Marie Gerats , Adrianus Johannes van Tunen , Michiel Marcel Albert Vandenbussche
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q2535/101 , C12Q2525/155 , C12Q2521/301 , C12Q2535/138
摘要： Method for the identification of a gene in a transposon population, comprising isolating genomic DNA, optionally pooling the DNA, restrict the DNA in the pools using an enzyme, ligate adaptors, amplifying the adaptor-ligated fragments with primers, one of which is a primer complementary to a border of a transposon sequence, high throughput sequencing of the fragments, aligning the fragments with known sequences in a database and thereby identifying gene candidates.
摘要翻译：用于鉴定转座子群体中的基因的方法，包括分离基因组DNA，任选地汇集DNA，使用酶限制DNA中的DNA，连接衔接子，用引物扩增衔接子连接的片段，其中之一是引物与转座子序列的边界互补，片段的高通量测序，将片段与数据库中的已知序列进行比对，从而鉴定候选基因。

4. 发明申请

US20090170713A1 HIGH THROUGHPUT SCREENING OF MUTAGENIZED POPULATIONS 审中-公开
标题翻译：繁殖人口的高通量筛选
公开(公告)号：US20090170713A1
公开(公告)日：2009-07-02
申请号：US12088794
申请日：2006-09-21
申请人： Michael Josephus Theresia van Eijk , Adrianus Johannes van Tunen
发明人： Michael Josephus Theresia van Eijk , Adrianus Johannes van Tunen
IPC分类号： C40B30/00 , C07H21/00
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6827 , C12Q1/6846 , C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , C12Q1/6869 , C12Q2600/13 , G16B30/00 , C12Q2525/155 , C12Q2563/179 , C12Q2563/155 , C12Q2537/143
摘要： Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
摘要翻译：公开了有效的方法用于在诱变的群体成员中基因突变的高通量鉴定。所述方法包括DNA分离，合并，扩增，文库的产生，文库的高通量测序，优选通过合成序列测序技术，鉴定突变和携带突变和识别突变的群体成员的鉴定。

5. 发明授权

US08614073B2 High throughput screening of mutagenized populations 有权
标题翻译：诱变人群的高通量筛选
公开(公告)号：US08614073B2
公开(公告)日：2013-12-24
申请号：US13447871
申请日：2012-04-16
申请人： Michael Josephus Theresia van Eijk , Adrianus Johannes van Tunen
发明人： Michael Josephus Theresia van Eijk , Adrianus Johannes van Tunen
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6827 , C12Q1/6846 , C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , C12Q1/6869 , C12Q2600/13 , G06F19/22 , C12Q2525/155 , C12Q2563/179 , C12Q2563/155 , C12Q2537/143
摘要： Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
摘要翻译：公开了有效的方法用于在诱变的群体成员中基因突变的高通量鉴定。所述方法包括DNA分离，合并，扩增，文库的产生，文库的高通量测序，优选通过合成序列测序技术，鉴定突变和携带突变和识别突变的群体成员的鉴定。

6. 发明授权

US08071310B2 Method for the high throughput screening of transposon tagging populations and massive parallel sequence identification of insertion sites 失效
标题翻译：用于转座子标记群体的高通量筛选和插入位点的大量并行序列鉴定的方法
公开(公告)号：US08071310B2
公开(公告)日：2011-12-06
申请号：US12093574
申请日：2006-11-08
申请人： Michael Josephus Theresia van Eijk , Antonius Gerardus Marie Gerats , Adrianus Johannes van Tunen , Michiel Marcel Albert Vandenbussche
发明人： Michael Josephus Theresia van Eijk , Antonius Gerardus Marie Gerats , Adrianus Johannes van Tunen , Michiel Marcel Albert Vandenbussche
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q2535/101 , C12Q2525/155 , C12Q2521/301 , C12Q2535/138
摘要： A method for the identification of a gene in a transposon population is provided. The method comprises isolating genomic DNA, optionally pooling the DNA, restricting the DNA in the pools using an enzyme, ligating adaptors, amplifying the adaptor-ligated fragments with primers one of which is a primer complementary to a border of a transposon sequence, sequencing the fragments using high throughput sequencing, aligning the fragments with known sequences in a database and thereby identifying gene candidates.
摘要翻译：提供了用于鉴定转座子群体中的基因的方法。该方法包括分离基因组DNA，任选地汇集DNA，使用酶限制DNA中的DNA，连接衔接子，用引物扩增衔接子连接的片段，其中一个引物是与转座子序列的边界互补的引物，测序使用高通量测序的片段，将片段与数据库中的已知序列进行比对，从而鉴定候选基因。

7. 发明授权

US08932812B2 Restriction enzyme based whole genome sequencing 有权
标题翻译：基于限制酶的全基因组测序
公开(公告)号：US08932812B2
公开(公告)日：2015-01-13
申请号：US13320379
申请日：2010-12-16
申请人： René Cornelis Josephus Hogers , Michael Josephus Theresia van Eijk
发明人： René Cornelis Josephus Hogers , Michael Josephus Theresia van Eijk
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6869 , C12Q2525/191 , C12Q2521/301
摘要： Method for de novo whole genome sequencing based on a (sequence-based) physical map of a DNA sample clone bank based on end-sequencing tagged adapter-ligated restriction fragments, in combination with sequencing adapter-ligated restriction fragments of the DNA sample wherein the recognition sequence of the restriction enzyme used in the generation of the physical map is identical to at least part of the recognition sequence of the restriction enzyme used in the generation of the DNA sample.
摘要翻译：基于基于末端测序标记衔接子连接的限制性片段的DNA样品克隆库的基于（基于序列的）物理图谱的新的全基因组测序的方法，与DNA样品的测序接头连接的限制性片段的组合，其中用于生成物理图谱的限制酶的识别序列与用于产生DNA样品的限制酶的识别序列的至少一部分相同。

8. 发明申请

US20120245037A1 RESTRICTION ENZYME BASED WHOLE GENOME SEQUENCING 有权
标题翻译：限制酶基因全基因组序列
公开(公告)号：US20120245037A1
公开(公告)日：2012-09-27
申请号：US13320379
申请日：2010-12-16
申请人： René Cornelis Josephus Hogers , Michael Josephus Theresia van Eijk
发明人： René Cornelis Josephus Hogers , Michael Josephus Theresia van Eijk
IPC分类号： C12Q1/68 , C40B20/00
CPC分类号： C12Q1/6869 , C12Q2525/191 , C12Q2521/301
摘要： Method for de novo whole genome sequencing based on a (sequence-based) physical map of a DNA sample clone bank based on end-sequencing tagged adapter-ligated restriction fragments, in combination with sequencing adapter-ligated restriction fragments of the DNA sample wherein the recognition sequence of the restriction enzyme used in the generation of the physical map is identical to at least part of the recognition sequence of the restriction enzyme used in the generation of the DNA sample.
摘要翻译：基于基于末端测序标记衔接子连接的限制性片段的DNA样品克隆库的基于（基于序列的）物理图谱的新的全基因组测序的方法，与DNA样品的测序接头连接的限制性片段的组合，其中用于生成物理图谱的限制酶的识别序列与用于产生DNA样品的限制酶的识别序列的至少一部分相同。

9. 发明申请

US20110287492A1 METHOD FOR THE REDUCTION OF REPETITIVE SEQUENCES IN ADAPTER-LIGATED RESTRICTION FRAGMENTS 审中-公开
标题翻译：减少适配器限制片段中重复序列的方法
公开(公告)号：US20110287492A1
公开(公告)日：2011-11-24
申请号：US13132698
申请日：2009-11-25
申请人： Daniele Trebbi , Michael Josephus Theresia van Eijk
发明人： Daniele Trebbi , Michael Josephus Theresia van Eijk
IPC分类号： C12P19/34
CPC分类号： C12N15/1093
摘要： The present invention relates to a method for the reduction of repetitive sequences (or the improvement in low-copy sequences) in DNA samples by a combination of restriction endonuclease treatment, followed by adapter ligation, renaturation kinetics-based fractionation, optionally coupled with duplex sequence nucleases and further restriction endonuclease treatment, followed by adapter ligation. The low-copy enriched fractions can be used in further DNA analysis.
摘要翻译：本发明涉及通过限制性内切核酸酶处理的组合来减少DNA样品中的重复序列（或低拷贝序列的改进）的方法，随后是衔接子连接，基于复性动力学的分级，任选与双链序列偶联核酸酶和进一步的限制性内切核酸酶处理，然后进行适配器连接。低拷贝浓缩的级分可用于进一步的DNA分析。

10. 发明申请

US20090142758A1 STRATEGIES FOR SEQUENCING COMPLEX GENOMES USING HIGH THROUGHPUT SEQUENCING TECHNOLOGIES 审中-公开
标题翻译：用于使用高通量测序技术测序复合基因的策略
公开(公告)号：US20090142758A1
公开(公告)日：2009-06-04
申请号：US11993961
申请日：2006-06-23
申请人： Michael Josephus Theresia van Eijk , Anker Preben Sorensen , Rene Cornelis Josephus Hogers
发明人： Michael Josephus Theresia van Eijk , Anker Preben Sorensen , Rene Cornelis Josephus Hogers
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191 , C12Q2521/301
摘要： A method for determining a genome sequence comprising the steps of digesting the genome with at least one first restriction endonuclease, ligating at least one adaptor to the restriction fragments of the first subset, selectively amplifying the first set of adaptor-ligated restriction fragments using a first primer combination wherein at least a first primer contains a first selected sequence at the 3′ end of the primer sequence, comprising 1-10 selective nucleotides, repeating these steps with at least a second primer combinations wherein the primer contains a different second selected sequence, fragmenting each of the subsets of amplified adaptor-ligated restriction fragments to generate sequencing libraries, determine the nucleotide sequence of the fragments, aligning the sequence of the fragments in each of the libraries to generate contigs, repeating these steps for one second and/or further restriction endonucleases, aligning the contigs obtained for each of the second and/or further restriction endonucleases to provide for a sequence of the genome.
摘要翻译：一种用于确定基因组序列的方法，包括以下步骤：用至少一个第一限制性内切核酸酶消化所述基因组，将至少一个衔接子与所述第一亚型的限制性片段连接，用第一组引物组合，其中至少第一引物在引物序列的3'末端含有第一选择的序列，包含1-10个选择性核苷酸，用至少第二引物组合重复这些步骤，其中引物含有不同的第二选择序列，将扩增的接头连接的限制性片段的每个子集分段以产生测序文库，确定片段的核苷酸序列，对齐每个文库中的片段序列以产生重叠群，重复这些步骤1秒和/或进一步限制性内切核酸酶，使得对于第二和/或毛皮中的每一个获得的重叠群限制性内切核酸酶以提供基因组的序列。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式