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1. 发明申请

US20090269749A1 METHOD FOR HIGH-THROUGHPUT AFLP-BASED POLYMORPHISM DETECTION 有权
标题翻译：用于高通量基于AFLP的多态性检测方法
公开(公告)号：US20090269749A1
公开(公告)日：2009-10-29
申请号：US12158040
申请日：2006-12-20
申请人： Michael Josephus Theresia Van Eijk , Anker Preben Sorensen , Marco Gerardus Maria Van Schriek
发明人： Michael Josephus Theresia Van Eijk , Anker Preben Sorensen , Marco Gerardus Maria Van Schriek
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
摘要： The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-) dominant genotypes of the genetic markers.
摘要翻译：本发明涉及一种用于一种或多种样品中一种或多种遗传标记物的高通量发现，检测和基因分型的方法，其包括DNA的限制性内切核酸酶消化，衔接子连接，任选的预扩增，选择性扩增，合并的扩增产物，对文库以足够的冗余进行测序，聚类，然后鉴定文库内和/或文库之间的遗传标记和确定遗传标记的（共）优势基因型。

2. 发明申请

US20090142758A1 STRATEGIES FOR SEQUENCING COMPLEX GENOMES USING HIGH THROUGHPUT SEQUENCING TECHNOLOGIES 审中-公开
标题翻译：用于使用高通量测序技术测序复合基因的策略
公开(公告)号：US20090142758A1
公开(公告)日：2009-06-04
申请号：US11993961
申请日：2006-06-23
申请人： Michael Josephus Theresia van Eijk , Anker Preben Sorensen , Rene Cornelis Josephus Hogers
发明人： Michael Josephus Theresia van Eijk , Anker Preben Sorensen , Rene Cornelis Josephus Hogers
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191 , C12Q2521/301
摘要： A method for determining a genome sequence comprising the steps of digesting the genome with at least one first restriction endonuclease, ligating at least one adaptor to the restriction fragments of the first subset, selectively amplifying the first set of adaptor-ligated restriction fragments using a first primer combination wherein at least a first primer contains a first selected sequence at the 3′ end of the primer sequence, comprising 1-10 selective nucleotides, repeating these steps with at least a second primer combinations wherein the primer contains a different second selected sequence, fragmenting each of the subsets of amplified adaptor-ligated restriction fragments to generate sequencing libraries, determine the nucleotide sequence of the fragments, aligning the sequence of the fragments in each of the libraries to generate contigs, repeating these steps for one second and/or further restriction endonucleases, aligning the contigs obtained for each of the second and/or further restriction endonucleases to provide for a sequence of the genome.
摘要翻译：一种用于确定基因组序列的方法，包括以下步骤：用至少一个第一限制性内切核酸酶消化所述基因组，将至少一个衔接子与所述第一亚型的限制性片段连接，用第一组引物组合，其中至少第一引物在引物序列的3'末端含有第一选择的序列，包含1-10个选择性核苷酸，用至少第二引物组合重复这些步骤，其中引物含有不同的第二选择序列，将扩增的接头连接的限制性片段的每个子集分段以产生测序文库，确定片段的核苷酸序列，对齐每个文库中的片段序列以产生重叠群，重复这些步骤1秒和/或进一步限制性内切核酸酶，使得对于第二和/或毛皮中的每一个获得的重叠群限制性内切核酸酶以提供基因组的序列。

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