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    • 8. 发明授权
    • Sunflower regeneration from cotyledons
    • 从子叶向日葵再生
    • US5030572A
    • 1991-07-09
    • US453155
    • 1989-12-14
    • Christopher J. PowerEbrahim Firoozabady
    • Christopher J. PowerEbrahim Firoozabady
    • C12N5/00C12N5/04
    • C12N5/0025C12N5/04
    • A method for regeneration of sunflower plants via shoot induction from cotyledon explants prepared from non-germinated sunflower embryos is described. Several methods for preparing explants by dissection of mature and immature fully-formed embryos are provided. Explants are prepared to insure that substantially all of the induced shoots derive from single cotyledon cells. Appropriately prepared explants are cultured on a shoot induction medium containing a combination of BA and NAA. Induced shoots are rooted and the resulting plantlets are grown into mature sunflower plants. This regeneration method is efficient, rapid and employs a convenient explant source, sunflower seed. This regeneration method is particularly useful in combination with methods for introduction of foreign DNA into sunflower cells and tissue.
    • 描述了通过从非发芽向日葵胚胎制备的子叶外植体通过芽诱导再生向日葵植物的方法。 提供了通过解剖成熟和未成熟的完整胚胎制备外植体的几种方法。 外植体准备确保基本上所有的诱导芽来源于单个子叶细胞。 将适当制备的外植体在含有BA和NAA的组合的芽诱导培养基上培养。 诱导芽生根,并将所得的小植株生长成成熟的向日葵植物。 这种再生方法是高效,快速的,并采用方便的外植体源,向日葵种子。 这种再生方法特别适用于将外源DNA引入向日葵细胞和组织的方法。