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    • 7. 发明授权
    • Debranching enzymes and DNA sequences coding them, suitable for changing
the degree of branching of amylopectin starch in plants
    • 提取酶和编码它们的DNA序列,适合于改变植物中支链淀粉的支化度
    • US6001628A
    • 1999-12-14
    • US596257
    • 1996-04-18
    • Jens KossmannMichael EmmermannIvar Virgin
    • Jens KossmannMichael EmmermannIvar Virgin
    • C12N15/09C12N5/10C12N9/44C12N15/56C12N15/82C12P19/04C07H21/04C12N1/20C12N15/00
    • C12N15/8245C12N9/2451
    • DNA sequences are described which on the codogenic strand code plant debranching enzymes, whose transcripts formed in transgenic plants code new proteins with the enzymatic activity of debranching enzymes which in transgenic plants reduce the degree of branching of amylopectin starch and DNA sequences which on the codogenic strand code plant debranching enzymes, whose transcripts formed in transgenic plants prevent the synthesis of proteins with the enzymatic activity of debranching enzymes, which in the transgenic plants increases the degree of branching of amylopectin starch, as well as plasmids on which these DNA sequences are localized, which can be introduced into plant cells and plants. Also described is a process for the production of plants changed by genetic engineering whose amylopectin starch is modified, and the modified starch obtainable from these plants.
    • PCT No.PCT / EP94 / 02623 Sec。 371日期:1996年4月18日 102(e)1996年4月18日PCT PCT 1994年8月8日PCT公布。 出版物WO95 / 04826 日期1995年2月16日描述了在编码植物脱支酶的编码基因编码植物脱支酶上的DNA序列,其在转基因植物中形成的转录物用转基因植物的酶活性代​​谢新的蛋白质,其在转基因植物中降低支链淀粉和DNA序列的支化度 其在代码链编码植物脱支酶上,其在转基因植物中形成的转录物阻止蛋白质的合成,其具有脱支酶的酶活性,其在转基因植物中增加了支链淀粉淀粉的支化度,以及这些质粒 DNA序列是局部化的,其可以被引入植物细胞和植物中。 还描述了由支链淀粉改性的遗传工程改变的植物的生产方法,以及可从这些植物获得的改性淀粉。