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1. 发明授权

US4645747A Specific binding assay technique 失效
标题翻译：特异性结合测定技术
公开(公告)号：US4645747A
公开(公告)日：1987-02-24
申请号：US470334
申请日：1983-02-28
申请人： Michael Cais , Moshe Shimoni , Abraham Baniel
发明人： Michael Cais , Moshe Shimoni , Abraham Baniel
IPC分类号： G01N33/537 , G01N33/538
CPC分类号： G01N33/5375 , Y10S436/807 , Y10S436/816 , Y10S436/817 , Y10S436/824
摘要： The present invention relates to a simple and accurate method to be applied in immunoassay technique for separating the free ligand from the antibody-bound ligand.The method involves the use of a solvent extraction operation which at equilibrium provides the formation of two distinct phases the free- and bound-fraction. The solvent to be utilized should be slightly water miscible or completely water immiscible, having the property of marked selective extraction power toward the gamma-labelled constituent to be determined. The method is applicable in a liquid-liquid system, a solid-liquid system and solid-solid system.The method is useful for radioimmunoassay, free radical assay or metallo-immunoassay, being most versatile having also the advantage that as a result of the free ligand being extracted into the solvent, the determination of the quantity of gamma-labelled substance in the free and/or bound fraction compares very favorably with the known prior art methods in immunoassays.
摘要翻译：本发明涉及将免疫测定技术应用于将游离配体与抗体结合的配体分离的简单而准确的方法。该方法包括使用溶剂萃取操作，其在平衡时提供形成两个不同相的游离和结合级分。待使用的溶剂应该是轻微的水混溶性或完全不混溶的水，具有显着选择性萃取力的特性，以朝向待测γ标记的成分。该方法适用于液液系统，固液系统和固体系统。该方法对于放射免疫测定，自由基测定或金属免疫测定是有用的，是最通用的，还具有以下优点：由于游离配体被提取到溶剂中，测定游离的γ标记物质的量 /或结合部分与免疫测定中已知的现有技术方法非常有利。

2. 发明授权

US4456690A Non-centrifugation method for immunoassay of materials 失效
标题翻译：材料免疫测定的非离心法
公开(公告)号：US4456690A
公开(公告)日：1984-06-26
申请号：US393397
申请日：1982-06-29
申请人： Michael Cais , Moshe Shimoni
发明人： Michael Cais , Moshe Shimoni
IPC分类号： A61K51/00 , G01N33/537 , G01N33/543 , G01N33/58 , G01N35/00 , G01T1/00
CPC分类号： G01N33/537 , Y10S436/80 , Y10S436/801 , Y10S436/804 , Y10S436/808 , Y10S436/809 , Y10S436/81 , Y10S436/814 , Y10S436/817 , Y10S436/824
摘要： The present invention relates to a method which eliminates centrifugation and decantation steps, to be performed in an automatic manner for carrying out specific binding assay tests, wherein liquid and solid phases are present.According to the invention, use is made of a specially designed device, consisting of a mixing reservoir into which is fitted snugly a mixer separator having a channel in the vertical axis of the mixer-separator. A rack holding a number of said mixing reservoirs containing the incubated reagents and analytes, capped with the mixer separators, is placed into a press-device designed to perform at a controlled rate a downward movement. The mixer separators are pushed downwards into the mixing reservoirs at a chosen rate for a preselected distance to complete the mass transport and separation operations. The separation devices are removed and either one of the separated phases can be measured in the desired analytical instrument for a quantitative or qualitative determination.The results obtained according to the present invention compare very favorably with those determined by other methods requiring centrifugation and/or decantation in the assay protocol.
摘要翻译：本发明涉及一种消除离心和倾析步骤的方法，其以自动方式进行以进行特定的结合测定试验，其中存在液相和固相。根据本发明，使用专门设计的装置，其由混合容器组成，混合容器紧密地装配有在混合器分离器的垂直轴线上具有通道的混合器分离器。将包含用混合器分离器封盖的温育的试剂和分析物的多个所述混合容器的架子放置在设计成以受控的速率执行向下运动的压力装置中。混合器分离器以选定的速率向下推入混合容器中以进行预选距离，以完成质量运输和分离操作。去除分离装置，并且可以在期望的分析仪器中测量分离相中的任何一个以进行定量或定性测定。根据本发明获得的结果与在测定方案中需要离心和/或倾析的其它方法测定的结果非常有利。

3. 发明授权

US4897193A Device for mass transport and separation through selective barriers 失效
标题翻译：通过选择性屏障进行大规模运输和分离的装置
公开(公告)号：US4897193A
公开(公告)日：1990-01-30
申请号：US303460
申请日：1989-01-31
申请人： Michael Cais , Moshe Shimoni
发明人： Michael Cais , Moshe Shimoni
IPC分类号： A61L2/02 , B01D11/02 , B01D11/04 , B01D15/08 , B01D33/01 , B01D35/00 , B01D61/00 , B01D61/02 , B01D61/08 , B01D61/10 , B01D61/42 , B01D61/58 , B01D63/00 , B01D63/16 , B01J19/00 , B01J20/02 , B01J47/00 , B01J47/12 , G01N25/14 , G01N30/52 , G01N33/53 , G01N33/537
CPC分类号： G01N33/537 , A61L2/022 , B01D11/02 , B01D11/0415 , B01D15/08 , B01D33/0183 , B01D61/025 , B01D61/08 , B01D63/08 , B01D63/087 , B01D2313/04 , B01D2313/50 , B01D2315/06 , B01D2317/025 , G01N2030/527 , Y10T436/255
摘要： The present invention relates to a new technique and device for mass transport operation and physical separation, being substantially quantitative and involving selective barriers. According to the new technique, the mass transport through the selective barrier and physical separation are carried out in the same device. The device consists of a mixing-reservoir into which is fitted snugly a mixer-separator, having a channel in the vertical axis of the mixer-separator, the mixer-separator being provided with means for accumulating a gas pocket to decrease the pressure exerted on the barrier. The completely solid-free liquid passing through the selective barrier is removed by pushing in the mixer-separator, said liquid being accumulated in a collecting container. Typical examples of such selective barriers are: membrane, bed of adsorbent material, paper, plastic, glass and metal filters or any other porous material, and hollow fibers. The new technique and device can be successfully utilized in any operation involving separation of solids from liquids and particularly useful in immunoassay, sterilization, ultra-filtration, desalination by reverse and osmosis.

4. 发明授权

US4454231A Method and device for mass transfer operation in immunoassays and other applications 失效
标题翻译：用于免疫测定和其他应用中质量传递操作的方法和装置
公开(公告)号：US4454231A
公开(公告)日：1984-06-12
申请号：US212806
申请日：1980-12-04
申请人： Michael Cais , Moshe Shimoni
发明人： Michael Cais , Moshe Shimoni
IPC分类号： B01D11/04 , B01J8/00 , B01J8/34 , G01N25/14 , G01N33/53 , G01N33/537 , G01N33/58 , B65D71/00 , G01N35/00
CPC分类号： B01D11/04 , B01J8/003 , B01J8/34 , G01N33/537 , B01L3/5021 , Y10S435/975 , Y10S436/80 , Y10S436/801 , Y10S436/804 , Y10S436/808 , Y10S436/81 , Y10S436/811 , Y10S436/816 , Y10S436/817
摘要： The present invention relates to a new technique and device for mass transfer of one or more components from one liquid phase to another liquid phase, involving a physical separation of said two phases.According to the new technique, the mass transfer and physical separation are carried out in the same device. The device consists of a mixing-reservoir into which is fitted snugly a mixer-separator, having a channel in the vertical axis of the mixer-separator. The two substantially immiscible liquid solutions are introduced into the mixing reservoir, the phases are thoroughly mixed, by moving the mixer-separator in and out the mixing reservoir. After the spontaneous separation into an upper and lower phase, the upper phase is removed by pushing in the mixer-separator said upper phase being accumulated in a collecting container.The new technique and device can be successfully utilized in the general field of liquid-liquid extraction and particularly applied in immunoassays, where the two basic operations of mass transfer and physical separation of fractions are required.
摘要翻译：本发明涉及用于将一种或多种组分从一个液相传质到另一个液相的新技术和装置，其涉及所述两相的物理分离。根据新技术，传质和物理分离在相同的装置中进行。该装置由混合容器组成，其中紧密地安装有混合器分离器，在混合器分离器的垂直轴上具有通道。将两个基本上不混溶的液体溶液引入混合容器中，通过将混合器分离器移入和移出混合容器而将相充分混合。在自发分离成上，下相之后，通过将混合器分离器中的上部相积聚在收集容器中来推动上相。新技术和设备可以在液 - 液萃取的一般领域得到成功应用，特别适用于免疫测定，其中需要传质和物理分离两种基本操作。

5. 发明授权

US4832850A Method and device for mass transport and separation through selective barriers 失效
标题翻译：通过选择性屏障进行质量运输和分离的方法和装置
公开(公告)号：US4832850A
公开(公告)日：1989-05-23
申请号：US821768
申请日：1985-12-27
申请人： Michael Cais , Moshe Shimoni
发明人： Michael Cais , Moshe Shimoni
IPC分类号： A61L2/02 , B01D11/02 , B01D11/04 , B01D15/08 , B01D33/01 , B01D35/00 , B01D61/00 , B01D61/02 , B01D61/08 , B01D61/10 , B01D61/42 , B01D61/58 , B01D63/00 , B01D63/16 , B01J19/00 , B01J20/02 , B01J47/00 , B01J47/12 , G01N25/14 , G01N30/52 , G01N33/53 , G01N33/537
CPC分类号： G01N33/537 , A61L2/022 , B01D11/02 , B01D11/0415 , B01D15/08 , B01D33/0183 , B01D61/025 , B01D61/08 , B01D63/08 , B01D63/087 , B01D2313/04 , B01D2313/50 , B01D2315/06 , B01D2317/025 , G01N2030/527 , Y10T436/255
摘要： The present invention relates to a new technique and device for mass transport operation and physical separation, being substantially quantitative and involving selective barriers. According to the new technique, the mass transport through the selective barrier and physical separation are carried out in the same device. The device consists of a mixing-reservoir into which is fitted snugly a mixer-separator, having a channel in the vertical axis of the mixer-separator, the mixer-separator being provided with means for accumulating a gas pocket to decrease the pressure exerted on the barrier. The completely solid-free liquid passing through the selective barrier is removed by pushing in the mixer-separator, said liquid being accumulated in a collecting container. Typical examples of such selective barriers are: membrane, bed of adsorbent material, paper, plastic, glass and metal filters or any other porous material, hollow fibers etc. The new technique and device can be successfully utilized in any operation involving separation of solids from liquids and particularly useful in immunoassay, sterilization, ultra-filtration, desalination by reverse osmosis, etc.
摘要翻译：本发明涉及一种用于大规模运输和物理分离的新技术和装置，其基本上是定量的并涉及选择性障碍物。根据新技术，通过选择性屏障和物理分离的质量传输在相同的装置中进行。该装置由混合容器组成，其中紧密地安装有混合器分离器，该混合器分离器在混合器分离器的垂直轴上具有通道，混合器分离器设置有用于累积气体袋以减小施加在屏障。通过推入混合器分离器来除去通过选择性屏障的完全无固体液体，所述液体积聚在收集容器中。这种选择性屏障的典型实例是：膜，吸附剂材料床，纸，塑料，玻璃和金属过滤器或任何其他多孔材料，中空纤维等。新技术和设备可以成功地用于任何操作，包括将固体从液体，特别适用于免疫测定，灭菌，超滤，反渗透脱盐等。

6. 发明授权

US4587221A Non-centrifugation method for immunoassay of materials 失效
标题翻译：材料免疫测定的非离心法
公开(公告)号：US4587221A
公开(公告)日：1986-05-06
申请号：US567003
申请日：1983-12-30
申请人： Michael Cais , Moshe Shimoni
发明人： Michael Cais , Moshe Shimoni
IPC分类号： A61K51/00 , G01N33/537 , G01N33/543 , G01N33/53 , B01D33/00 , G01N33/536
CPC分类号： G01N33/537 , Y10S436/80 , Y10S436/801 , Y10S436/804 , Y10S436/808 , Y10S436/809 , Y10S436/81 , Y10S436/814 , Y10S436/817 , Y10S436/824
摘要： The present invention relates to a method which eliminates centrifugation and decantation steps, to be performed in an automatic manner for carrying out specific binding assay tests, wherein liquid and solid phases are present.According to the invention, use is made of a specially designed device, consisting of a mixing reservoir into which is fitted snugly a mixer separator having a channel in the vertical axis of the mixer-separator. A rack holding a number of said mixing reservoirs containing the incubated reagents and analytes, capped with the mixer separators, is placed into a press-device designed to perform at a controlled rate a downward movement. The mixer separators are pushed downwards into the mixing reservoirs at a chosen rate for a preselected distance to complete the mass transport and separation operations. The separation devices are removed and either one of the separated phases can be measured in the desired analytical instrument for a quantitative or qualitative determination.The results obtained according to the present invention compare very favorably with those determined by other methods requiring centrifugation and/or decantation in the assay protocol.
摘要翻译：本发明涉及一种消除离心和倾析步骤的方法，其以自动方式进行以进行特定的结合测定试验，其中存在液相和固相。根据本发明，使用专门设计的装置，其由混合容器组成，混合容器紧密地装配有在混合器分离器的垂直轴线上具有通道的混合器分离器。将包含用混合器分离器封盖的温育的试剂和分析物的多个所述混合容器的架子放置在设计成以受控的速率执行向下运动的压力装置中。混合器分离器以选定的速率向下推入混合容器中以进行预选距离，以完成质量运输和分离操作。去除分离装置，并且可以在期望的分析仪器中测量分离相中的任何一个以进行定量或定性测定。根据本发明获得的结果与在测定方案中需要离心和/或倾析的其它方法测定的结果非常有利。

7. 发明授权

US4510058A Method for a new type of chromatography and device therefor 失效
标题翻译：新型色谱法及其设备的方法
公开(公告)号：US4510058A
公开(公告)日：1985-04-09
申请号：US518811
申请日：1983-07-29
申请人： Michael Cais , Moshe Shimoni
发明人： Michael Cais , Moshe Shimoni
IPC分类号： B01D15/02 , B01D15/08 , G01N30/02 , G01N30/46 , G01N30/58 , G01N30/60 , G01N33/543
CPC分类号： G01N30/58 , B01D15/1892 , B01D15/1885 , G01N30/461 , G01N30/6004 , G01N30/6091
摘要： The present invention relates to a new type of chromatography technique, referred to as dynamic column chromatography, for separation of one or more compounds present in a solution, which is characterized by the existence of a moving solid adsorbent bed. According to the invention, the chromatographic system comprises a piston having at its bottom a sealing element and a longitudinal channel containing the adsorbent between two barriers and a test tube having at its bottom a multiple way valve. By pushing the piston into the test tube, the desired eluent which was prior forced through said valve is entering under intrinsic pressure of the closed system through the channel moving the adsorbed compounds to be separated between said barriers, the solution obtained going out through a nozzle located at one of the end parts of the piston. The dynamic column liquid chromatography is applicable to: silica gel chromatography, reversed phase liquid chromatography, affinity chromatography, capillary chromatography, chromato-focusing, gel filtration and ion exchange chromatography. In the dynamic column liquid chromatography, the equilibrium distribution of the compounds between the adsorbent and liquid is established very rapidly, resulting in sharp and narrow zones of the separated fractions.
摘要翻译：本发明涉及用于分离存在于溶液中的一种或多种化合物的新型色谱技术（称为动态柱色谱法），其特征在于存在移动的固体吸附剂床。根据本发明，色谱系统包括在其底部具有密封元件的活塞和在两个屏障之间包含吸附剂的纵向通道和在其底部具有多重阀的试管。通过将活塞推入试管中，先前强制通过所述阀的所需洗脱液通过所述通道移动被封闭系统的固有压力进入所述阻挡层之间被分离的吸附化合物，通过喷嘴获得的溶液位于活塞的一个端部。动态柱色谱法适用于硅胶色谱，反相液相色谱，亲和层析，毛细管色谱，色谱聚焦，凝胶过滤和离子交换色谱。在动态柱液相色谱中，化合物在吸附剂和液体之间的平衡分布非常迅速地建立，导致分离的馏分的尖锐和窄的区域。

8. 发明授权

US4205952A Specific binding assay method and reagent means 失效
标题翻译：特异性结合法和试剂方法
公开(公告)号：US4205952A
公开(公告)日：1980-06-03
申请号：US795457
申请日：1977-05-10
申请人： Michael Cais
发明人： Michael Cais
IPC分类号： C07D239/62 , C07D311/80 , C07F11/00 , C07F13/00 , C07F17/02 , C07J1/00 , C07J41/00 , C07J43/00 , C07J51/00 , G01N33/532 , G01N33/536 , G01N33/543 , G01N33/16
CPC分类号： G01N33/532 , C07D239/62 , C07D311/80 , C07F11/00 , C07F13/00 , C07F17/02 , C07J1/007 , C07J41/0011 , C07J41/0072 , C07J41/0088 , C07J43/003 , C07J51/00 , Y10S436/801 , Y10S436/805 , Y10S436/816 , Y10S436/817
摘要： A method and reagent means for the determination of small quantities of chemical compounds in man, animal and plants by a specific binding assay technique.The method involves the utilization of a labelling constituent comprising a conjugate of a labelling substance and a binding component said labelling substance being one or more metal atoms which can be easily determined. The labelling constituents are selected from various metalo organic derivatives or metal coordination complexes.The method is very specific and sensitive and can be successfully utilized for the determination of haptens selected from various groups such as alkaloids, barbiturates, steroids, cannabinoids, vitamins, aminoacids, tranquilizers, sugars, penicillins, etc.
摘要翻译：用于通过特异性结合测定技术测定人，动物和植物中少量化合物的方法和试剂方法。该方法包括利用包含标记物质和结合成分的缀合物的标记成分，所述标记物质是易于测定的一种或多种金属原子。标记成分选自各种金属有机衍生物或金属配位络合物。该方法非常特异和灵敏，可以成功地用于测定从生物碱，巴比妥类，类固醇，大麻素，维生素，氨基酸，镇定剂，糖，青霉素等各种组中选择的半抗原。

9. 发明授权

US4230664A Test pack kit for immunoassay 失效
标题翻译：用于免疫测定的测试包试剂盒
公开(公告)号：US4230664A
公开(公告)日：1980-10-28
申请号：US5754
申请日：1979-01-23
申请人： Michael Cais
发明人： Michael Cais
IPC分类号： B01L3/14 , G01N33/532 , G01N33/537 , G01N33/16
CPC分类号： B01L3/5082 , G01N33/532 , G01N33/537 , Y10S436/801 , Y10S436/808 , Y10S436/81
摘要： A test pack kit for assaying for a ligand in a liquid medium. The kit comprises specially designed assay tubes, each tube having a cylindrical side arm which can be interconnected with the side arm of another assay tube. In addition, the pack kit contains antiserum; a conjugate of a metal labelling substance with the ligand; assay calibration standards--in vials containing known concentrations of unlabelled ligand; a separation agent in vials containing material used for separating the bound antibody--metal labelling complex from the unbound metal labelling substance and instrument calibration standards--in vials containing several known concentrations of the metal--labelling substance. Errors caused by pipetting or transferring of reagents from one container to another are practically eliminated.
摘要翻译：用于测定液体介质中配体的测试包试剂盒。该试剂盒包括特别设计的测定管，每个管具有可与另一测定管的侧臂互连的圆柱形侧臂。此外，包装盒含有抗血清; 金属标记物质与配体的共轭物; 测定校准标准品 - 含有已知浓度的未标记配体的小瓶; 在包含用于从未结合的金属标记物质和仪器校准标准物分离结合的抗体 - 金属标记复合物的材料的小瓶中的分离剂 - 在含有几种已知浓度的金属标记物质的小瓶中。实际上消除了将试剂从一个容器移液或转移到另一个容器造成的错误。

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