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1. 发明申请

US20110281266A1 Identification of Nucleic Acids 审中-公开
标题翻译：核酸鉴定
公开(公告)号：US20110281266A1
公开(公告)日：2011-11-17
申请号：US13106736
申请日：2011-05-12
申请人： Nikolay V. Sergeev , Maxim G. Brevnov , Manohar R. Furtado
发明人： Nikolay V. Sergeev , Maxim G. Brevnov , Manohar R. Furtado
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6818 , C12Q1/686 , Y02A50/52 , C12Q2565/1025 , C12Q2563/173 , C12Q2527/107 , C12Q2561/101
摘要： This disclosure relates to methods for identifying target nucleic acids in a sample by detecting an amplified sequence corresponding to the target using a detectable probe and by monitoring its melting temperature (Tm).
摘要翻译：本公开涉及通过使用可检测的探针检测对应于靶的扩增序列并通过监测其解链温度（Tm）来鉴定样品中的靶核酸的方法。

2. 发明申请

US20100029925A1 METHODS AND KITS FOR EXTRACTION OF DNA 审中-公开
标题翻译：提取DNA的方法和工具
公开(公告)号：US20100029925A1
公开(公告)日：2010-02-04
申请号：US12471325
申请日：2009-05-22
申请人： Maxim G. Brevnov , Jaiprakash Shewale , Manohar R. Furtado , Hemant S. Pawar
发明人： Maxim G. Brevnov , Jaiprakash Shewale , Manohar R. Furtado , Hemant S. Pawar
IPC分类号： C07H1/00 , C07H21/04
CPC分类号： C12N15/1013
摘要： Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.
摘要翻译：公开了用于从溶液中回收生物聚合物的方法和材料。特别地，本发明提供从生物材料中提取和分离核酸的方法。在洗涤剂和溶剂的存在下，可以通过与可溶性多糖聚合物和磁性颗粒形成稳定的络合物来分离核酸。当颗粒从溶液中磁性分离出来时，核酸与它们分离。随后可以将核酸从颗粒中释放并分离出来。核酸制备物可用于实现下游分子技术的有效和准确的结果，例如定量，核酸来源的鉴定和基因分型。

3. 发明授权

US08568580B2 Systems and methods for isolating nucleic acids 失效
标题翻译：用于分离核酸的系统和方法
公开(公告)号：US08568580B2
公开(公告)日：2013-10-29
申请号：US12179455
申请日：2008-07-24
申请人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
发明人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
IPC分类号： G01N27/453 , B01D61/42
CPC分类号： C12N15/101 , G01N27/44782
摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.
摘要翻译：用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间所述至少一个样品室。该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场，其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

4. 发明授权

US08497065B2 Methods and kits for extraction of DNA 有权
标题翻译：提取DNA的方法和试剂盒
公开(公告)号：US08497065B2
公开(公告)日：2013-07-30
申请号：US12371515
申请日：2009-02-13
申请人： Maxim G. Brevnov , Hemant S. Pawar , Manohar R. Furtado , Jaiprakash Shewale
发明人： Maxim G. Brevnov , Hemant S. Pawar , Manohar R. Furtado , Jaiprakash Shewale
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1013 , C12N15/1006 , C12Q1/6806 , C12Q2563/143 , C12Q2523/308
摘要： Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.
摘要翻译：公开了用于从溶液中回收生物聚合物的方法和材料。特别地，本发明提供从生物材料中提取和分离核酸的方法。在洗涤剂和溶剂的存在下，可以通过与可溶性多糖聚合物和磁性颗粒形成稳定的络合物来分离核酸。当颗粒从溶液中磁性分离出来时，核酸与它们分离。随后可以将核酸从颗粒中释放并分离出来。核酸制备物可用于实现下游分子技术的有效和准确的结果，例如定量，核酸来源的鉴定和基因分型。

5. 发明申请

US20110318743A1 METHODS, WORKFLOWS, KITS, APPARATUSES, AND COMPUTER PROGRAM MEDIA FOR NUCLEIC ACID SAMPLE PREPARATION FOR NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：用于核酸序列的核酸样品制备的方法，工作流程，工具，设备和计算机程序介质
公开(公告)号：US20110318743A1
公开(公告)日：2011-12-29
申请号：US13171322
申请日：2011-06-28
申请人： Maxim G. Brevnov , Elena Bolchakova , Manohar Furtado
发明人： Maxim G. Brevnov , Elena Bolchakova , Manohar Furtado
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/6869 , C12Q2563/131 , C12Q2563/143
摘要： A method for preparing a nucleic acid sample for nucleic acid sequencing includes amplifying a nucleic acid target sequence using a primer bound to a first capture substrate; capturing an amplified nucleic acid product by the first capture substrate; generating at least one sequencing ladder from the amplified nucleic acid product using at least one sequencing primer; capturing the at least one sequencing ladder by hybridizing the at least one sequencing ladder to a complementary capture compound on a second capture substrate; and removing the at least one sequencing ladder from the second capture substrate. The first and/or second capture substrate may include a magnetic particle. Other methods, workflows, kits, and computer program media for nucleic acid sample preparation are also disclosed.
摘要翻译：用于制备用于核酸测序的核酸样品的方法包括使用与第一捕获底物结合的引物扩增核酸靶序列; 通过第一捕获基质捕获扩增的核酸产物; 使用至少一个测序引物从扩增的核酸产物产生至少一个测序梯; 通过将所述至少一个测序梯度杂交到第二捕获基底上的互补捕获化合物来捕获所述至少一个测序梯; 以及从所述第二捕获基板去除所述至少一个测序梯。第一和/或第二捕获基板可以包括磁性颗粒。还公开了用于核酸样品制备的其它方法，工作流程，试剂盒和计算机程序培养基。

6. 发明申请

US20090209751A1 METHODS AND KITS FOR EXTRACTION OF DNA 有权
标题翻译：提取DNA的方法和工具
公开(公告)号：US20090209751A1
公开(公告)日：2009-08-20
申请号：US12371515
申请日：2009-02-13
申请人： Maxim G. Brevnov , Hemant S. Pawar , Manohar R. Furtado , Jaiprakash Shewale
发明人： Maxim G. Brevnov , Hemant S. Pawar , Manohar R. Furtado , Jaiprakash Shewale
IPC分类号： C07H1/00 , C07H21/04
CPC分类号： C12N15/1013 , C12N15/1006 , C12Q1/6806 , C12Q2563/143 , C12Q2523/308
摘要： Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.
摘要翻译：公开了用于从溶液中回收生物聚合物的方法和材料。特别地，本发明提供从生物材料中提取和分离核酸的方法。在洗涤剂和溶剂的存在下，可以通过与可溶性多糖聚合物和磁性颗粒形成稳定的络合物来分离核酸。当颗粒从溶液中磁力分离出来时，核酸与它们分离。随后可以将核酸从颗粒中释放并分离出来。核酸制备物可用于实现下游分子技术的有效和准确的结果，例如定量，核酸来源的鉴定和基因分型。

7. 发明申请

US20090071830A1 Systems and Methods for Isolating Nucleic Acids 失效
标题翻译：用于分离核酸的系统和方法
公开(公告)号：US20090071830A1
公开(公告)日：2009-03-19
申请号：US12179455
申请日：2008-07-24
申请人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
发明人： Charles S. Vann , Maxim G. Brevnov , David W. Ruff , Kenneth J. Livak
IPC分类号： B01D57/02 , B01D61/42 , C25B9/08
CPC分类号： C12N15/101 , G01N27/44782
摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.
摘要翻译：用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间所述至少一个样品室。该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场，其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

8. 发明授权

US06942974B2 Selective elution of immobilized multiplexed primer extension products 有权
标题翻译：固定化多重引物延伸产物的选择性洗脱
公开(公告)号：US06942974B2
公开(公告)日：2005-09-13
申请号：US10096176
申请日：2002-03-08
申请人： Maxim G. Brevnov
发明人： Maxim G. Brevnov
IPC分类号： B01D20060101 , B01D1/00 , C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2565/519 , C12Q2563/149 , C12Q2537/161 , C12Q2565/537 , C12Q2537/149
摘要： The present invention provides methods and kits for the sequential elution of families of extension products from one or more substrates. The methods and kits can be used, for example, to separate families of primer extension products generated by multiplex sequencing reactions or by other multiplex primer extension reactions.
摘要翻译：本发明提供了用于从一个或多个底物顺序洗脱延伸产物家族的方法和试剂盒。所述方法和试剂盒可用于例如分离由多重测序反应或其它多重引物延伸反应产生的引物延伸产物的家族。

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