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1. 发明申请

US20100286380A1 PRETREATMENT METHOD FOR EXTRACTION OF NUCLEIC ACID FROM BIOLOGICAL SAMPLES AND KITS THEREFOR 审中-公开
标题翻译：从生物样品中提取核酸的预处理方法及其制备方法
公开(公告)号：US20100286380A1
公开(公告)日：2010-11-11
申请号：US12778501
申请日：2010-05-12
申请人： Matthew P. Collis , Donald Copertino , Karen Eckert , Thomas Fort
发明人： Matthew P. Collis , Donald Copertino , Karen Eckert , Thomas Fort
IPC分类号： C07H21/02 , C12N1/08 , C12N9/00
CPC分类号： C12Q1/6806 , C12Q2523/113 , C12Q2527/101 , C12Q2527/125
摘要： The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
摘要翻译：本发明涉及用于从中提取核酸的生物样品的预处理方法。本发明使用至少一种蛋白质变性剂与一种或多种以下元素的组合来形成用于提取核酸的反应混合物：（1）至少一种非质子溶剂，（2）逐步加热，和（3）样品稀释。

2. 发明授权

US07601491B2 Pretreatment method for extraction of nucleic acid from biological samples and kits therefor 失效
标题翻译：从生物样品中提取核酸的预处理方法及其试剂盒
公开(公告)号：US07601491B2
公开(公告)日：2009-10-13
申请号：US10359179
申请日：2003-02-06
申请人： Matthew P. Collis , Donald W. Copertino , Karen Eckert , Thomas L. Fort
发明人： Matthew P. Collis , Donald W. Copertino , Karen Eckert , Thomas L. Fort
IPC分类号： C12Q1/68 , C07H21/00 , C07K14/00
CPC分类号： C12Q1/6806 , C12Q2523/113 , C12Q2527/101 , C12Q2527/125
摘要： The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
摘要翻译：本发明涉及用于预处理提取核酸的生物样品的方法本发明采用至少一种蛋白质变性剂与一种或多种以下元素的组合形成用于提取核酸的反应混合物：（1）至少一种非质子溶剂，（2）逐步加热，和（3）样品稀释。

3. 发明授权

US07727727B2 Pretreatment method for extraction of nucleic acid from biological samples and kits therefor 失效
标题翻译：从生物样品中提取核酸的预处理方法及其试剂盒
公开(公告)号：US07727727B2
公开(公告)日：2010-06-01
申请号：US12359854
申请日：2009-01-26
申请人： Matthew P. Collis , Donald W. Copertino , Karen Eckert , Thomas L. Fort
发明人： Matthew P. Collis , Donald W. Copertino , Karen Eckert , Thomas L. Fort
IPC分类号： C12Q1/68 , B01L3/00 , C07H19/00 , C07K2/00
CPC分类号： C12Q1/6806 , C12Q2523/113 , C12Q2527/101 , C12Q2527/125
摘要： The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
摘要翻译：本发明涉及用于从中提取核酸的生物样品的预处理方法。本发明使用至少一种蛋白质变性剂与一种或多种以下元素的组合来形成用于提取核酸的反应混合物：（1）至少一种非质子溶剂，（2）逐步加热，和（3）样品稀释。

4. 发明授权

US06258546B1 Detection of nucleic acid amplification 有权
标题翻译：检测核酸扩增
公开(公告)号：US06258546B1
公开(公告)日：2001-07-10
申请号：US09602996
申请日：2000-06-23
申请人： Ray A. McMillian , Karen Eckert , Donald W. Copertino , Tobin J. Hellyer
发明人： Ray A. McMillian , Karen Eckert , Donald W. Copertino , Tobin J. Hellyer
IPC分类号： C12Q168
CPC分类号： C12Q1/6818 , C12Q1/6844 , C12Q2531/119 , C12Q2525/301 , C12Q2521/301 , C12Q2565/1025
摘要： For use in nucleic acid amplification reactions, the detector oligonucleotides of the invention comprise a target binding sequence which is at least partially the same as the target binding sequence of an amplification primer present in the target amplification reaction, so that the detector oligonucleotide and the amplification primer compete for hybridization to the same sequence in the target. Hybridization of the amplification primer to the target upstream from the detector oligonucleotide generates a nickable restriction endonuclease recognition site. When this site is nicked and strand displacement occurs from the nick, both the 3′ end of the amplification primer and the detector oligonucleotide are displaced. The displaced detector oligonucleotide may then be detected as an indication of the presence of the target sequence, for example by unfolding of a fluorescently labeled secondary structure present in the detector oligonucleotide to reduce fluorescence quenching.
摘要翻译：为了用于核酸扩增反应，本发明的检测寡核苷酸包含与目标扩增反应中存在的扩增引物的靶结合序列至少部分相同的靶结合序列，使得检测寡核苷酸和扩增引物竞争与目标中相同序列的杂交。扩增引物与检测寡核苷酸上游的靶的杂交产生可切口的限制性内切核酸酶识别位点。当该位点被切口并且从切口发生链置换时，扩增引物的3'末端和检测器寡核苷酸都被置换。然后可以将位移的检测器寡核苷酸作为目标序列存在的指示进行检测，例如通过解折叠存在于检测器寡核苷酸中的荧光标记的二级结构以减少荧光猝灭。

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