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1. 发明申请

US20080108112A1 Apparatus and methods for parallel processing of micro-volume liquid reactions 有权
标题翻译：用于平行处理微量液体反应的装置和方法
公开(公告)号：US20080108112A1
公开(公告)日：2008-05-08
申请号：US12004116
申请日：2007-12-20
申请人： Matthew O'keefe , Pamela Foreman
发明人： Matthew O'keefe , Pamela Foreman
IPC分类号： C12P19/34 , C12M1/00
CPC分类号： B01L3/50857 , B01F13/0059 , B01F13/0071 , B01F13/0084 , B01F15/0201 , B01F15/0232 , B01F2215/0431 , B01J2219/00286 , B01J2219/00317 , B01J2219/0072 , B01J2219/00722 , B01J2219/0074 , B01L3/5025 , B01L3/5088 , B01L2200/0642 , B01L2300/0819 , B01L2300/0829 , B01L2300/087 , B01L2300/10 , B01L2300/165 , B01L2400/0688 , C40B40/06 , C40B60/14 , H01J49/0418 , Y02P20/582
摘要： Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.
摘要翻译：本文公开了用于以阵列格式进行多次同时微量化学和生化反应的装置和方法。在一个实施例中，格式包括衬底中的微孔阵列。除了作为样品室的有序阵列允许进行多个并行反应之外，阵列还可用于试剂储存和转移，库显示，试剂合成，组装多个相同的反应，稀释和脱盐。使用阵列有助于反应的光学分析，并允许实时进行光学分析。包括在本发明中的是包括微孔装置和在装置中要执行的方法的反应组分的试剂盒。

2. 发明申请

US20050148066A1 Apparatus and methods for parallel processing of micro-volume liquid reactions 有权
标题翻译：用于平行处理微量液体反应的装置和方法
公开(公告)号：US20050148066A1
公开(公告)日：2005-07-07
申请号：US11054184
申请日：2005-02-09
申请人： Matthew O'Keefe , Pamela Foreman
发明人： Matthew O'Keefe , Pamela Foreman
IPC分类号： B01F13/00 , B01F15/02 , B01L3/00 , C40B40/06 , C40B60/14 , H01J49/04 , C12M1/34
CPC分类号： C12Q1/686 , B01F13/0059 , B01F13/0071 , B01F13/0084 , B01F15/0201 , B01F15/0232 , B01F2215/0431 , B01J2219/00286 , B01J2219/00317 , B01J2219/0072 , B01J2219/00722 , B01J2219/0074 , B01L3/5025 , B01L3/50857 , B01L3/5088 , B01L2200/0642 , B01L2300/0819 , B01L2300/0829 , B01L2300/087 , B01L2300/10 , B01L2300/165 , B01L2400/0688 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2600/172 , C40B40/06 , C40B60/14 , H01J49/0418 , Y02P20/582
摘要： Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.
摘要翻译：本文公开了用于以阵列格式进行多次同时微量化学和生化反应的装置和方法。在一个实施例中，格式包括衬底中的微孔阵列。除了作为样品室的有序阵列允许进行多个并行反应之外，阵列还可用于试剂储存和转移，库显示，试剂合成，组装多个相同的反应，稀释和脱盐。使用阵列有助于反应的光学分析，并允许实时进行光学分析。包括在本发明中的是包括微孔装置和在装置中要执行的方法的反应组分的试剂盒。

3. 发明申请

US20100203593A1 CIP1 Polypeptides and their uses 有权
标题翻译： CIP1多肽及其用途
公开(公告)号：US20100203593A1
公开(公告)日：2010-08-12
申请号：US12683108
申请日：2010-01-06
申请人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
发明人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
IPC分类号： C12P19/00 , C12N9/24 , C12N9/42
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译：本文描述了从里氏木霉分离的新基因序列。描述编码包含纤维素结合结构域的蛋白质的两个基因，一个编码阿拉伯糖呋喃糖苷酶的基因和一个编码乙酰木聚糖酯酶的基因。序列CIP1和CIP2包含纤维素结合结构域。这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。

4. 发明申请

US20110287511A1 Novel Trichoderma Genes 有权
标题翻译：新型木霉属基因
公开(公告)号：US20110287511A1
公开(公告)日：2011-11-24
申请号：US13029919
申请日：2011-02-17
申请人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
发明人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
IPC分类号： C12N9/14 , C07H21/02 , C12N1/15 , C07H21/04 , C12N15/63 , C12N1/00
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译：本文描述了从里氏木霉分离的新基因序列。描述编码包含纤维素结合结构域的蛋白质的两个基因，一个编码阿拉伯糖呋喃糖苷酶的基因和一个编码乙酰木聚糖酯酶的基因。序列CIP1和CIP2含有纤维素结合结构域。这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。

5. 发明授权

US07923235B2 CIP1 polypeptides and their uses 有权
标题翻译： CIP1多肽及其用途
公开(公告)号：US07923235B2
公开(公告)日：2011-04-12
申请号：US12683108
申请日：2010-01-06
申请人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
发明人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
IPC分类号： C12N9/00 , C12N9/24 , C12N1/20 , C12N15/00 , C12N15/74 , C07H21/04 , C11D3/00
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译：本文描述了从里氏木霉分离的新基因序列。描述编码包含纤维素结合结构域的蛋白质的两个基因，一个编码阿拉伯糖呋喃糖苷酶的基因和一个编码乙酰木聚糖酯酶的基因。序列CIP1和CIP2含有纤维素结合结构域。这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。

6. 发明授权

US08202704B2 Trichoderma genes 有权
标题翻译：木霉属基因
公开(公告)号：US08202704B2
公开(公告)日：2012-06-19
申请号：US13029919
申请日：2011-02-17
申请人： Pamela Foreman , Frits Goedegebuur , Pieter Van Solingen , Michael Ward
发明人： Pamela Foreman , Frits Goedegebuur , Pieter Van Solingen , Michael Ward
IPC分类号： C12P21/06 , C12N9/00 , C12N9/24 , C12N1/20 , C12N15/00
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译：本文描述了从里氏木霉分离的新基因序列。描述编码包含纤维素结合结构域的蛋白质的两个基因，一个编码阿拉伯糖呋喃糖苷酶的基因和一个编码乙酰木聚糖酯酶的基因。序列CIP1和CIP2含有纤维素结合结构域。这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。

7. 发明授权

US07666648B2 Isolated polypeptide having arabinofuranosidase activity 有权
公开(公告)号：US07666648B2
公开(公告)日：2010-02-23
申请号：US10555358
申请日：2004-05-28
申请人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
发明人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
IPC分类号： C12N9/00 , C12N9/24 , C12N1/20 , C12N15/00 , C12N15/74 , C11D3/00 , C07H21/04
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry. cip1 cDNA sequence (SEQ ID NO: 1) GACTAGTTCA TAATACAGTA GTTGAGTTCA TAGCAACTTC50 ACTCTCTAGC TGAACAAATT ATCTGCGCAA ACATGGTTCG CCGGACTGCT100 CTGCTGGCCC TTGGGGCTCT CTCAACGCTC TCTATGGCCC AAATCTCAGA150 CGACTTCGAG TCGGGCTGGG ATCAGACTAA ATGGCCCATT TCGGCACCAG200 ACTGTAACCA GGGCGGCACC GTCAGCCTCG ACACCACAGT AGCCCACAGC250 GGCAGCAACT CCATGAAGGT CGTTGGTGGC CCCAATGGCT ACTGTGGACA300 CATCTTCTTC GGCACTACCC AGGTGCCAAC TGGGGATGTA TATGTCAGAG350 CTTGGATTCG GCTTCAGACT GCTCTCGGCA GCAACCACGT CACATTCATC400 ATCATGCCAG ACACCGCTCA GGGAGGGAAG CACCTCCGAA TTGGTGGCCA450 AAGCCAAGTT CTCGACTACA ACCGCGAGTC CGACGATGCC ACTCTTCCGG500 ACCTGTCTCC CAACGGCATT GCCTCCACCG TCACTCTGCC TACCGGCGCG550 TTCCAGTGCT TCGAGTACCA CCTGGGCACT GACGGAACCA TCGAGACGTG600 GCTCAACGGC AGCCTCATCC CGGGCATGAC CGTGGGCCCT GGCGTCGACA650 ATCCAAACGA CGCTGGCTGG ACGAGGGCCA GCTATATTCC GGAGATCACC700 GGTGTCAACT TTGGCTGGGA GGCCTACAGC GGAGACGTCA ACACCGTCTG750 GTTCGACGAC ATCTCGATTG CGTCGACCCG CGTGGGATGC GGCCCCGGCA800 GCCCCGGCGG TCCTGGAAGC TCGACGACTG GGCGTAGCAG CACCTCGGGC850 CCGACGAGCA CTTCGAGGCC AAGCACCACC ATTCCGCCAC CGACTTCCAG900 GACAACGACC GCCACGGGTC CGACTCAGAC ACACTATGGC CAGTGCGGAG1000 GGATTGGTTA CAGCGGGCCT ACGGTCTGCG CGAGCGGCAC GACCTGCCAG1050 GTCCTGAACC CATACTACTC CCAGTGCTTA TAAGGGGATG AGCATGGAGT1100 GAAGTGAAGT GAAGTGGAGA GAGTTGAAGT GGCATTGCGC TCGGCTGGGT1150 AGATAAAAGT CAGCAGCTAT GAATACTCTA TGTGATGCTC ATTGGCGTGT1200 ACGTTTTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA1250 AAAAAAAAAA AAAAAAAAAG GGGGCGGCCG C1271

8. 发明申请

US20070184463A1 Microfluidic device for purifying a biological component using magnetic beads 审中-公开
标题翻译：用于使用磁珠净化生物成分的微流体装置
公开(公告)号：US20070184463A1
公开(公告)日：2007-08-09
申请号：US11542652
申请日：2006-10-02
申请人： Josh Molho , Pamela Foreman
发明人： Josh Molho , Pamela Foreman
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： G01N1/34 , B01L3/502761 , B01L2200/027 , B01L2200/0668 , B01L2300/0816 , B01L2400/0415 , B01L2400/0487 , G01N35/0098
摘要： A method of purifying a biological component found in a biological sample by extracting the biological component from the biological sample. The method is performed using a microfluidic device having at least one well for receiving the biological sample and at least one channel for introducing and removing fluids. A plurality of magnetic beads having a factor with an affinity for the biological component is introduced to the well together with a suitable biological sample. The biological sample is manipulated to release the biological component in proximity to the magnetic beads which are then segregated within the well while removing the biological sample. An elution solution for the biological component is introduced to the well and the elution solution together with the biological component are withdrawn therefrom.
摘要翻译：一种通过从生物样品提取生物成分来纯化生物样品中生物成分的方法。该方法使用具有至少一个孔的微流体装置进行，用于接收生物样品和用于引入和去除流体的至少一个通道。将具有对生物成分具有亲和性的因子的多个磁珠与合适的生物样品一起引入到孔中。操作生物样品以释放靠近磁珠的生物组分，然后在除去生物样品之前将其分离在孔内。将生物成分的洗脱溶液引入到孔中，从生物成分中抽出洗脱溶液。

9. 发明申请

US20070128690A1 Novel trichoderma genes 有权
标题翻译：新型木霉基因
公开(公告)号：US20070128690A1
公开(公告)日：2007-06-07
申请号：US10555358
申请日：2004-05-28
申请人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
发明人： Pamela Foreman , Pieter Van Solingen , Frits Goedegebuur , Michael Ward
IPC分类号： C11D3/386 , C12N9/42 , C12P21/06 , C07H21/04 , C12N1/16 , C12N15/74
CPC分类号： C07K14/37 , C11D3/38645 , C12N9/18 , C12Y302/01004 , C12Y302/01091 , Y02E50/16
摘要： Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
摘要翻译：本文描述了从里氏木霉分离的新基因序列。描述编码包含纤维素结合结构域的蛋白质的两个基因，一个编码阿拉伯糖呋喃糖苷酶的基因和一个编码乙酰木聚糖酯酶的基因。序列CIP1和CIP2含有纤维素结合结构域。这些蛋白质在纺织和洗涤剂行业以及纸浆和造纸工业中特别有用。

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