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1. 发明授权

US08809053B2 Cardiomyocyte culture support 有权
标题翻译：心肌细胞培养支持
公开(公告)号：US08809053B2
公开(公告)日：2014-08-19
申请号：US12186572
申请日：2008-08-06
申请人： Masanao Watanabe , Teruo Okano , Masayuki Yamato , Tatsuya Shimizu , Yoshikatsu Akiyama
发明人： Masanao Watanabe , Teruo Okano , Masayuki Yamato , Tatsuya Shimizu , Yoshikatsu Akiyama
IPC分类号： C12N5/07
CPC分类号： C12N5/0657 , C12N2533/30
摘要： Methods and reagents are described for myocardial regenerative therapy using cardiomyocytes that are cultured ex vivo to have a controlled orientation of beating. This is achieved by growing the cardiomyocytes on a culture support that is coated with a polymerizable liquid crystal which is oriented so as to be in a liquid crystal phase state prior to curing with ionizing radiation or ultraviolet rays.
摘要翻译：描述了使用心肌细胞进行心肌再生治疗的方法和试剂，所述心肌细胞离体培养以具有受控的打浆取向。这是通过在涂覆有可聚合液晶的培养支持物上生长心肌细胞来实现的，该可聚合液晶在用电离辐射或紫外线固化之前被定向为处于液晶相状态。

2. 发明申请

US20080131476A1 Cultured Cell Sheet, Production Method and Tissue Repair Method Using Thereof 审中-公开
标题翻译：培养细胞片，生产方法和组织修复方法
公开(公告)号：US20080131476A1
公开(公告)日：2008-06-05
申请号：US11885222
申请日：2006-02-28
申请人： Masato Kanzaki , Masayuki Yamato , Tinatsu Kohno , Hidekazu Sekine , Akihiko Kikuchi , Teruo Okano , Takamasa Onuki
发明人： Masato Kanzaki , Masayuki Yamato , Tinatsu Kohno , Hidekazu Sekine , Akihiko Kikuchi , Teruo Okano , Takamasa Onuki
IPC分类号： C12N5/06 , A61F2/00
CPC分类号： A61L27/3895 , A61L27/34 , A61L27/3804 , A61L27/3839 , C12N5/0068 , C12N5/0688 , C12N2533/30
摘要： It is intended to provide a cultured cell sheet with excellent tissue adherence and flexibility. The above object can be achieved by culturing cells on a support for cell culture in which a surface of a substrate is coated with a temperature-responsive polymer whose lower or upper critical solution temperature against water is in the range of 0 and 80° C. along with a surfactant protein or a crosslinking inhibitor and producing a cultured cell sheet by detaching it by setting the temperature of the culture to the upper critical solution temperature or higher or to the lower critical solution temperature or lower.
摘要翻译：旨在提供具有优异组织粘附性和柔韧性的培养细胞片。上述目的可以通过在细胞培养支持体上培养细胞来实现，其中基质的表面被温度响应性聚合物涂覆，其温度较低或上限临界溶解温度对水在0和80℃的范围内。以及表面活性剂蛋白质或交联抑制剂，通过将培养物的温度设定在上限临界溶液温度以上或降低临界溶解温度以下，通过分离培养的细胞片材。

3. 发明申请

US20080118474A1 Cultured Periodontal Membrane Cell Sheet, Process for Producing the Same and Method of Use Thereof 有权
标题翻译：培养的牙周膜细胞片，其制备方法及其使用方法
公开(公告)号：US20080118474A1
公开(公告)日：2008-05-22
申请号：US11587427
申请日：2005-04-25
申请人： Teruo Okano , Masateru Hasegawa , Masayuki Yamato , Akihiko Kikuchi , Isao Ishikawa
发明人： Teruo Okano , Masateru Hasegawa , Masayuki Yamato , Akihiko Kikuchi , Isao Ishikawa
IPC分类号： A01N63/00 , A01N1/00 , C12N5/06
CPC分类号： A61L27/3886 , A61C8/0006 , A61K35/32 , A61L27/3804 , A61L27/3865 , C12N5/066 , C12N2502/097 , C12N2502/1311 , C12N2513/00 , C12N2539/10
摘要： A cultured periodontal ligament cell sheet is produced by a process comprising culturing cells on a cell culture support composed of a base material whose surface is coated with a temperature-responsive polymer having an upper limit or lower limit critical dissolution temperature in water of 0 to 80° C. under specified conditions, (1) regulating the temperature of a culture fluid to the upper limit critical dissolution temperature or higher or to the lower limit critical temperature or lower, (2) allowing a cultured periodontal ligament cell sheet resulting from the culturing to adhere to a carrier and (3) detaching the sheet intact together with the carrier. This cultured periodontal ligament cell sheet has a syndesmotic microstructure, exhibits extremely high bioadherence to the dental root surface and realizes high density transplantation of target cells and positive reconstruction of periodontal tissues. Moreover, through lamination of cell sheets to be transplanted and donation of a three-dimensional polarity, highly efficient reconstruction of an adhesion apparatus can be accomplished and clinical application thereof to moderate periodontitis, severe peridontitis, gingival recession and the like is highly promising.
摘要翻译：培养的牙周韧带细胞片通过以下方法制备：将细胞培养在由表面涂覆有在水中的上限或下限临界溶解温度为0〜80的温度敏感性聚合物的基材构成的细胞培养支持体上 ℃，（1）将培养液的温度调节至上限临界溶解温度或更高或下限临界温度或更低，（2）允许培养产生的培养的牙周膜细胞片粘贴在载体上，（3）与载体一起分离片材。这种培养的牙周膜细胞片具有相似的微观结构，对牙根表面具有极高的生物相干性，实现了靶细胞的高密度移植和牙周组织的积极重建。此外，通过层叠待移植的细胞片和三维极性的提供，可以实现粘附装置的高效重建，并且其适度的牙周炎，严重的周围炎，牙龈退缩等的临床应用是非常有希望的。

4. 发明申请

US20120210451A1 ISLET CELL SHEET, PROCESS FOR PRODUCTION THEREOF, AND USE THEREOF 审中-公开
标题翻译：印刷电路板，其生产工艺及其使用
公开(公告)号：US20120210451A1
公开(公告)日：2012-08-16
申请号：US13388535
申请日：2010-08-02
申请人： Hirofumi Shimizu , Kazuo Ohashi , Rie Utoh , Kazuya Ise , Masayuki Yamato , Teruo Okano , Mitsukazu Gotoh
发明人： Hirofumi Shimizu , Kazuo Ohashi , Rie Utoh , Kazuya Ise , Masayuki Yamato , Teruo Okano , Mitsukazu Gotoh
IPC分类号： A61K35/39 , C12N5/071 , A61P1/18 , G01N21/64 , A61P3/10 , A61K9/00 , A01K67/027
CPC分类号： A61L27/3804 , A61L27/3604 , A61L27/38 , C12N5/0676 , C12N2533/30 , C12N2533/52 , C12N2539/10
摘要： A polymer that changes hydration at a temperature between 0 to 80° C. is coated on the surface of a cell culture support, and islet cells are cultured on the support at a temperature range that causes polymer to have weak hydration, then the temperature of a culture solution is changed to a temperature that causes the polymer to have strong hydration to obtain islet cells in a sheet form. Such islet cells in a sheet form have an insulin producing function even if there is no blood flow.
摘要翻译：将在0至80℃温度下变化水合的聚合物涂覆在细胞培养载体的表面上，并且在使得聚合物具有弱水合的温度范围内将胰岛细胞在载体上培养，然后将温度将培养溶液变为导致聚合物具有强水合作用以获得片状的胰岛细胞的温度。即使没有血液流动，片状的这种胰岛细胞也具有产生胰岛素的功能。

5. 发明授权

US08642338B2 Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same 有权
标题翻译：前眼部相关细胞片，三维结构及其制备方法
公开(公告)号：US08642338B2
公开(公告)日：2014-02-04
申请号：US10544542
申请日：2003-02-06
申请人： Masayuki Yamato , Teruo Okano
发明人： Masayuki Yamato , Teruo Okano
IPC分类号： C12N11/08 , C12N5/00 , C12N5/02
CPC分类号： C12N5/0621 , A61K35/12
摘要： An anterior ocular segment related cell sheet or three-dimensional structure that have only a few structural defects as they have been recovered retaining the intercellular desmosome structure and the basement membrane-like protein between cell and substrate. The anterior ocular segment related cell sheet or three-dimensional structure is produced by a process comprising the steps of cultivating cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer having an upper or lower critical dissolution temperature of 0-80° C. with respect to water, optionally stratifying the layer of cultured cells by the usual method, and thereafter, (1) adjusting the temperature of the culture solution to either above the upper critical dissolution temperature or below the lower critical dissolution temperature, and further optionally (2) bringing the cultured anterior ocular segment related cell sheet or three-dimensional structure into close contact with a polymer membrane, and (3) detaching the sheet or three-dimensional structure together with the polymer membrane.
摘要翻译：前眼部相关的细胞片或三维结构只有几个结构缺陷，因为它们已经被回收，保留了细胞和基质之间的细胞间质结构和基底膜样蛋白。前眼部相关细胞片或三维结构的制备方法包括以下步骤：在细胞培养支持物上培养细胞，所述细胞培养载体包含其表面被温度响应性聚合物覆盖的基底，所述温度响应聚合物具有上或下临界溶解温度为0 -80℃，任选地通过常规方法分层培养细胞层，然后，（1）将培养溶液的温度调节至高于上临界溶解温度或低于临界溶解温度，进一步任选地（2）使培养的眼前段相关细胞片或三维结构与聚合物膜紧密接触，和（3）与聚合物膜一起分离片或三维结构。

6. 发明申请

US20130289723A1 MIDDLE EAR MUCOSA-LIKE CELL SHEET, PROCESS OF PRODUCING THE SAME AND METHOD OF USING THE SAME 审中-公开
标题翻译：中等耳状细胞片及其制造方法及其使用方法
公开(公告)号：US20130289723A1
公开(公告)日：2013-10-31
申请号：US13823240
申请日：2011-09-15
申请人： Takanori Hama , Hiromi Kojima , Hiroshi Moriyama , Masayuki Yamato , Teruo Okano
发明人： Takanori Hama , Hiromi Kojima , Hiroshi Moriyama , Masayuki Yamato , Teruo Okano
IPC分类号： A61F2/18
CPC分类号： A61F2/18 , A61F2002/183 , A61F2310/00365 , A61K35/12 , A61L27/3804 , A61L27/3813 , A61L27/3839 , A61L27/3886 , A61L2430/14 , C12N5/062 , C12N2533/30
摘要： The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cell sir the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80 ° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.
摘要翻译：本发明的目的是获得中耳粘膜替代物，其置于中耳骨表面上，肉芽组织和骨骼的增生以及成纤维细胞的发育中耳的中耳被抑制，并获得在表层中保留纤毛的中耳粘膜样细胞片，包括在涂覆有聚合物的细胞培养基质上培养鼻上皮细胞，其水合力在0至 80℃，其中在聚合物的水合力弱的温度范围内培养细胞，然后将温度改变到水合力强的温度以回收培养的细胞片。

7. 发明申请

US20110207220A1 CELL PATTERN RECOVERY TOOL 有权
标题翻译：细胞图案恢复工具
公开(公告)号：US20110207220A1
公开(公告)日：2011-08-25
申请号：US13125491
申请日：2009-08-11
申请人： Teruo Okano , Masayuki Yamato , Tatsuya Shimizu , Masatoshi Kuroda
发明人： Teruo Okano , Masayuki Yamato , Tatsuya Shimizu , Masatoshi Kuroda
IPC分类号： C12N5/071
CPC分类号： C12N11/02 , C12M23/20 , C12M33/00 , C12N5/0062 , C12N2535/10 , C12N2539/10
摘要： This invention provides a cell pattern recovery tool comprising a base material layer having a surface subjected to easy adhesion treatment, a temperature responsive polymer layer that is provided on the base material layer and has a surface subjected to silane treatment, and a cell adhesion inhibiting material layer provided on the temperature responsive polymer layer. According to the present invention, a cell pattern can be rapidly recovered while maintaining the cell pattern stably and reliably under minimally invasive conditions for the cells.
摘要翻译：本发明提供了一种电池图案回收工具，其包括具有易于粘附处理的表面的基材层，设置在基材层上并具有进行硅烷处理的表面的温度响应性聚合物层和细胞粘附抑制材料提供在温度敏感聚合物层上的层。根据本发明，可以在针对细胞的微创条件下稳定且可靠地保持细胞图案，能够快速地恢复细胞图案。

8. 发明申请

US20080289052A1 Method of Constructing Animal Having Cancer Cells Transplanted Thereinto 审中-公开
标题翻译：构建具有移植癌细胞的动物的方法
公开(公告)号：US20080289052A1
公开(公告)日：2008-11-20
申请号：US10591612
申请日：2005-03-04
申请人： Teruo Okano , Akihiko Kikuchi , Masayuki Yamato , Akira Masuda
发明人： Teruo Okano , Akihiko Kikuchi , Masayuki Yamato , Akira Masuda
IPC分类号： G01N33/15 , A01K67/027
CPC分类号： A01K67/0271 , A01K2227/105 , A01K2267/0331 , A61K49/0008 , C12N5/0693
摘要： A cell culture support is first prepared that is coated on a surface with a polymer the hydration force of which changes in a temperature range of 0-80° C.; cancer cells are then cultivated on the support in a temperature region where the polymer has weak hydration force; thereafter, the culture solution is adjusted to a temperature at which the polymer has a stronger hydration force, whereby the cultured cancer cells are detached; the detached cancer cells are then transplanted to a specified site of an animal on which transplantation is to be performed; this method is an efficient way of cancer cells transplantation.
摘要翻译：首先制备细胞培养载体，其涂覆在表面上，其水合力在0-80℃的温度范围内变化; 然后将癌细胞在聚合物具有弱水合力的温度区域中在载体上培养; 此后，将培养液调整为聚合物具有更强的水合力的温度，由此分离培养的癌细胞。然后将分离的癌细胞移植到要进行移植的动物的特定部位; 这种方法是癌细胞移植的有效途径。

9. 发明申请

US20080009063A1 SUBSTRATE FOR CELL CULTURE 审中-公开
标题翻译：细胞培养基质
公开(公告)号：US20080009063A1
公开(公告)日：2008-01-10
申请号：US11773127
申请日：2007-07-03
申请人： Teruo Okano , Masayuki Yamato , Yoshikatsu Akiyama , Masanao Watanabe , Hiroyuki Naganuma , Masami Nara
发明人： Teruo Okano , Masayuki Yamato , Yoshikatsu Akiyama , Masanao Watanabe , Hiroyuki Naganuma , Masami Nara
IPC分类号： C12N5/02
CPC分类号： C12N5/0068 , C12M25/00 , C12M25/06 , C12N2533/30 , C12N2535/00 , C12N2535/10 , C12N2539/10
摘要： An object of the present invention is to provide a cell culture substrate capable of quickly forming a cell sheet and easily removing the cell sheet after formed. The present invention relates to a cell culture substrate for forming a cell sheet by culturing cells, comprising a plurality of projections each having a top face and depressions formed between the projections, in which the depressions each have an opening whose dimensions are too small for a cell to be cultured to enter and the cell sheet is removable. The present invention further relates to a method of preparing a cell sheet using the substrate and a cell sheet prepared by the method.
摘要翻译：本发明的目的是提供能够快速形成细胞片并且容易地除去细胞片的细胞培养基材。本发明涉及用于通过培养细胞形成细胞片的细胞培养基质，所述细胞培养基底包括多个突起，每个突起具有顶表面和在所述突起之间形成的凹陷，其中所述凹陷各自具有尺寸对于要培养的细胞进入，细胞片是可移除的。本发明还涉及使用该基材制备细胞片的方法和通过该方法制备的细胞片。

10. 发明授权

US09127270B2 Cell pattern recovery tool 有权
标题翻译：细胞图案恢复工具
公开(公告)号：US09127270B2
公开(公告)日：2015-09-08
申请号：US13125491
申请日：2009-08-11
申请人： Teruo Okano , Masayuki Yamato , Tatsuya Shimizu , Masatoshi Kuroda
发明人： Teruo Okano , Masayuki Yamato , Tatsuya Shimizu , Masatoshi Kuroda
IPC分类号： C12N5/071 , C12N11/02 , C12N5/00
CPC分类号： C12N11/02 , C12M23/20 , C12M33/00 , C12N5/0062 , C12N2535/10 , C12N2539/10
摘要： This invention provides a cell pattern recovery tool comprising a base material layer having a surface subjected to easy adhesion treatment, a temperature responsive polymer layer that is provided on the base material layer and has a surface subjected to silane treatment, and a cell adhesion inhibiting material layer provided on the temperature responsive polymer layer. According to the present invention, a cell pattern can be rapidly recovered while maintaining the cell pattern stably and reliably under minimally invasive conditions for the cells.
摘要翻译：本发明提供了一种电池图案回收工具，其包括具有易于粘附处理的表面的基材层，设置在基材层上并具有进行硅烷处理的表面的温度响应性聚合物层和细胞粘附抑制材料提供在温度敏感聚合物层上的层。根据本发明，可以在针对细胞的微创条件下稳定且可靠地保持细胞图案，能够快速地恢复细胞图案。

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