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    • 4. 发明授权
    • Process for purification of proanthocyanidin oligomer
    • 纯化原花青素低聚物的方法
    • US06800433B1
    • 2004-10-05
    • US09959274
    • 2001-10-19
    • Shinkichi HondaTomoya TakahashiAyako KamimuraTakako MatsuokaTomomasa KandaAkio YanagidaKazuo Hieda
    • Shinkichi HondaTomoya TakahashiAyako KamimuraTakako MatsuokaTomomasa KandaAkio YanagidaKazuo Hieda
    • C12Q100
    • C07D311/62C07D311/60
    • The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises extracting the proanthocyanidin oligomers from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by solid-liquid extraction using methyl acetate as a liquid phase; a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these; and a process for purifying dimeric to pentameric proanthocyanidin oligomers with a uniform polymerization degree which comprises separating and purifying the proanthocyanidin oligomers by polymerization degree from raw materials containing the proanthocyanidin oligomers or crude purification products there from by normal phase silica gel liquid chromatography using as a mobile phase a single solvent or a mixed solvent of two or more solvents selected from the group consisting of an ester solvent, a ketone solvent, a hydrocarbon solvent, an ether solvent and an alcohol solvent.
    • 本发明涉及一种纯化二聚至五聚原花青素低聚物的方法,该方法包括通过使用乙酸甲酯作为液相通过固液萃取从原料中提取原花青素低聚物或粗提纯产物; 用于纯化二聚体至五聚原花青素低聚物的方法,其包括用酶预处理含有原花青素低聚物的水解原料,其粗提纯产物或含有这些之一的溶液; 以及具有均匀聚合度的二聚至五聚原花青素低聚物的纯化方法,该方法包括通过使用作为流动相的正相硅胶液相色谱法从含有原花青素低聚物或粗纯化产物的原料中分离纯化原花青素低聚物 将溶剂选自酯溶剂,酮溶剂,烃溶剂,醚溶剂和醇溶剂中的两种或更多种溶剂的单一溶剂或混合溶剂。