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1. 发明申请

US20090035846A1 DEVICE FOR MEASURING EXTRACELLULAR POTENTIAL AND METHOD OF MANUFACTURING DEVICE 有权
标题翻译：用于测量细胞外潜能的装置和制造装置的方法
公开(公告)号：US20090035846A1
公开(公告)日：2009-02-05
申请号：US12245091
申请日：2008-10-03
申请人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
发明人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
IPC分类号： C12M1/34
CPC分类号： G01N33/48728 , G01N33/54353 , G01N33/552
摘要： A device for measuring an extracellular potential of a test cell includes a substrate having a well formed in a first surface thereof and a first trap hole formed therein. The well has a bottom. The first trap hole includes a first opening formed in the bottom of the well and extending toward a second face of the substrate, a first hollow section communicating with the first opening via a first connecting portion, and a second opening extending reaching the second surface and communicating with the first hollow section via a second connecting portion. The first connecting portion has a diameter smaller than a maximum diameter of the first hollow section, greater than a diameter of the second connecting portion, and smaller than a diameter of the test cell. The device can retain the test cell securely and accept chemicals and the test cell to be put into the device easily.
摘要翻译：用于测量测试电池的细胞外电位的装置包括在其第一表面中形成良好的衬底和形成在其中的第一陷阱。井有一个底部。第一陷阱包括形成在井的底部并朝向基板的第二面延伸的第一开口，经由第一连接部分与第一开口连通的第一中空部分，以及延伸到第二表面的第二开口，通过第二连接部与第一中空部连通。第一连接部分的直径小于第一中空部分的最大直径，大于第二连接部分的直径，并且小于测试电池的直径。该装置可以牢固地保持测试电池，并容易接受化学品和测试电池放入设备中。

2. 发明申请

US20090239033A1 DIAPHRAGM AND DEVICE FOR MEASURING CELLULAR POTENTIAL USING THE SAME, MANUFACTURING METHOD OF THE DIAPHRAGM 有权
标题翻译：用于测量细胞膜的膜片和装置，其制造方法
公开(公告)号：US20090239033A1
公开(公告)日：2009-09-24
申请号：US12359426
申请日：2009-01-26
申请人： Masaya Nakatani , Soichiro Hiraoka , Hiroshi Ushio , Akiyoshi Oshima , Hiroaki Oka , Fumiaki Emoto
发明人： Masaya Nakatani , Soichiro Hiraoka , Hiroshi Ushio , Akiyoshi Oshima , Hiroaki Oka , Fumiaki Emoto
IPC分类号： B32B3/10 , B44C1/22
CPC分类号： G01N33/48728 , Y10T428/24322
摘要： A diaphragm is formed by etching a substrate. This substrate has a first surface provided with a depression by isotropic dry etching, and a second surface opposite the first surface. Furthermore, a through-hole is formed from the depression to the second surface by anisotropic dry etching. The depression and the through-hole are formed by using one resist mask. The depression has a hemispherical shape or a semi-elliptical spherical shape.
摘要翻译：通过蚀刻基板形成隔膜。该基板具有通过各向同性干蚀刻设置有凹部的第一表面和与第一表面相对的第二表面。此外，通过各向异性干蚀刻从凹陷形成通孔到第二表面。通过使用一个抗蚀剂掩模形成凹陷和通孔。凹陷具有半球形或半椭圆形球形。

3. 发明授权

US08257962B2 Extracellular potential measuring device and its manufacturing method 有权
标题翻译：细胞外电位测定装置及其制造方法
公开(公告)号：US08257962B2
公开(公告)日：2012-09-04
申请号：US10513392
申请日：2004-03-08
申请人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
发明人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
IPC分类号： C12M1/42 , C12M3/00 , C12N13/00
CPC分类号： G01N33/48728
摘要： An extracellular potential measuring device includes a plate portion having a first surface and a second surface opposite to the first surface, and an electrode provided on the second surface of the plate portion. In the plate portion, a pocket having an opening which opens to the first surface is formed, and a through-hole communicating to the second surface from the pocket. The through-hole communicates from a position which is closer to the opening than a deepest point of the first pocket. The electrode is provided around of the opening of the through-hole. In this device, even if a cell to be examined does not reach the deepest point of the pocket, a cell membrane of the cell can tightly attaches onto the through-hole securely without a clearance. Hence, culture solution inside the through-hole is isolated from culture solution over an upper surface of the plate portion, thereby allowing electrochemical changes caused by activities of the cell to be detected efficiently with a detector electrode.
摘要翻译：细胞外电位测量装置包括具有第一表面和与第一表面相对的第二表面的板部分，以及设置在板部分的第二表面上的电极。在该板部分中，形成具有向第一表面开口的开口的口袋，以及从口袋连通到第二表面的通孔。通孔从比第一口袋的最深点更靠近开口的位置连通。电极设置在通孔的开口周围。在该装置中，即使要检查的细胞未达到口袋的最深点，细胞的细胞膜可以牢固地紧贴在通孔上而没有间隙。因此，通孔内部的培养液在培养液的上表面上与培养液分离，从而能够通过检测器电极有效地检测细胞活性引起的电化学变化。

4. 发明授权

US08202439B2 Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm 有权
标题翻译：膜片和用于测量细胞电位的装置使用相同的膜片的制造方法
公开(公告)号：US08202439B2
公开(公告)日：2012-06-19
申请号：US12359426
申请日：2009-01-26
申请人： Masaya Nakatani , Soichiro Hiraoka , Hiroshi Ushio , Akiyoshi Oshima , Hiroaki Oka , Fumiaki Emoto
发明人： Masaya Nakatani , Soichiro Hiraoka , Hiroshi Ushio , Akiyoshi Oshima , Hiroaki Oka , Fumiaki Emoto
IPC分类号： B44C1/22 , H01L21/302
CPC分类号： G01N33/48728 , Y10T428/24322
摘要： A diaphragm is formed by etching a substrate. This substrate has a first surface provided with a depression by isotropic dry etching, and a second surface opposite the first surface. Furthermore, a through-hole is formed from the depression to the second surface by anisotropic dry etching. The depression and the through-hole are formed by using one resist mask. The depression has a hemispherical shape or a semi-elliptical spherical shape.
摘要翻译：通过蚀刻基板形成隔膜。该基板具有通过各向同性干蚀刻设置有凹部的第一表面和与第一表面相对的第二表面。此外，通过各向异性干蚀刻从凹陷形成通孔到第二表面。通过使用一个抗蚀剂掩模形成凹陷和通孔。凹陷具有半球形或半椭圆形球形。

5. 发明授权

US08232084B2 Device for measuring extracellular potential and method of manufacturing device 有权
标题翻译：用于测量细胞外电位的装置和制造装置的方法
公开(公告)号：US08232084B2
公开(公告)日：2012-07-31
申请号：US12245091
申请日：2008-10-03
申请人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
发明人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
IPC分类号： C12N13/00 , C12M1/42 , C12M3/00
CPC分类号： G01N33/48728 , G01N33/54353 , G01N33/552
摘要： A device for measuring an extracellular potential of a test cell includes a substrate having a well formed in a first surface thereof and a first trap hole formed therein. The well has a bottom. The first trap hole includes a first opening formed in the bottom of the well and extending toward a second face of the substrate, a first hollow section communicating with the first opening via a first connecting portion, and a second opening extending reaching the second surface and communicating with the first hollow section via a second connecting portion. The first connecting portion has a diameter smaller than a maximum diameter of the first hollow section, greater than a diameter of the second connecting portion, and smaller than a diameter of the test cell. The device can retain the test cell securely and accept chemicals and the test cell to be put into the device easily.
摘要翻译：用于测量测试电池的细胞外电位的装置包括在其第一表面中形成良好的衬底和形成在其中的第一陷阱。井有一个底部。第一陷阱包括形成在井的底部并朝向基板的第二面延伸的第一开口，经由第一连接部分与第一开口连通的第一中空部分和延伸到第二表面的第二开口，通过第二连接部与第一中空部连通。第一连接部分的直径小于第一中空部分的最大直径，大于第二连接部分的直径，并且小于测试电池的直径。该装置可以牢固地保持测试电池，并容易接受化学品和测试电池放入设备中。

6. 发明授权

US07501278B2 Extracellular potential measuring device and method for fabricating the same 有权
标题翻译：细胞外电位测定装置及其制造方法
公开(公告)号：US07501278B2
公开(公告)日：2009-03-10
申请号：US10485644
申请日：2003-06-02
申请人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
发明人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
IPC分类号： C12M1/36 , C12M1/38 , C12M3/00 , C12M1/34 , C12M1/00
CPC分类号： G01N33/48728 , G01N33/54353 , G01N33/552
摘要： A device for measuring an extracellular potential of a test cell includes a substrate having a well formed in a first surface thereof and a first trap hole formed therein. The well has a bottom. The first trap hole includes a first opening formed in the bottom of the well and extending toward a second face of the substrate, a first hollow section communicating with the first opening via a first connecting portion, and a second opening extending reaching the second surface and communicating with the first hollow section via a second connecting portion. The first connecting portion has a diameter smaller than a maximum diameter of the first hollow section, greater than a diameter of the second connecting portion, and smaller than a diameter of the test cell. The device can retain the test cell securely and accept chemicals and the test cell to be put into the device easily.
摘要翻译：用于测量测试电池的细胞外电位的装置包括在其第一表面中形成良好的衬底和形成在其中的第一陷阱。井有一个底部。第一陷阱包括形成在井的底部并朝向基板的第二面延伸的第一开口，经由第一连接部分与第一开口连通的第一中空部分和延伸到第二表面的第二开口，通过第二连接部与第一中空部连通。第一连接部分的直径小于第一中空部分的最大直径，大于第二连接部分的直径，并且小于测试电池的直径。该装置可以牢固地保持测试电池，并容易接受化学品和测试电池放入设备中。

7. 发明申请

US20050221469A1 Extracellular potential measuring device and its manufacturing method 有权
标题翻译：细胞外电位测定装置及其制造方法
公开(公告)号：US20050221469A1
公开(公告)日：2005-10-06
申请号：US10513392
申请日：2004-03-08
申请人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
发明人： Masaya Nakatani , Hiroaki Oka , Fumiaki Emoto
IPC分类号： G01N33/487 , C12M1/34
CPC分类号： G01N33/48728
摘要： An extracellular potential measuring device includes a plate portion having a first surface and a second surface opposite to the first surface, and an electrode provided on the second surface of the plate portion. In the plate portion, a pocket having an opening which opens to the first surface is formed, and a through-hole communicating to the second surface from the pocket. The through-hole communicates from a position which is closer to the opening than a deepest point of the first pocket. The electrode is provided around of the opening of the through-hole. In this device, even if a cell to be examined does not reach the deepest point of the pocket, a cell membrane of the cell can tightly attaches onto the through-hole securely without a clearance. Hence, culture solution inside the through-hole is isolated from culture solution over an upper surface of the plate portion, thereby allowing electrochemical changes caused by activities of the cell to be detected efficiently with a detector electrode.
摘要翻译：细胞外电位测量装置包括具有第一表面和与第一表面相对的第二表面的板部分，以及设置在板部分的第二表面上的电极。在该板部分中，形成具有向第一表面开口的开口的口袋，以及从口袋连通到第二表面的通孔。通孔从比第一口袋的最深点更靠近开口的位置连通。电极设置在通孔的开口周围。在该装置中，即使要检查的细胞未达到口袋的最深点，细胞的细胞膜可以牢固地紧贴在通孔上而没有间隙。因此，通孔内部的培养液在培养液的上表面上与培养液分离，从而能够通过检测器电极有效地检测细胞活性引起的电化学变化。

8. 发明授权

US08247218B2 Extracellular potential sensing element, device for measuring extracellular potential, apparatus for measuring extracellular potential and method of measuring extracellular potential by using the same 有权
标题翻译：细胞外电位感测元件，用于测量细胞外电位的装置，用于测量细胞外电位的装置和使用该细胞外电位测量细胞外电位的方法
公开(公告)号：US08247218B2
公开(公告)日：2012-08-21
申请号：US12133452
申请日：2008-06-05
申请人： Masaya Nakatani , Nobuhiko Ozaki , Hiroaki Oka
发明人： Masaya Nakatani , Nobuhiko Ozaki , Hiroaki Oka
IPC分类号： C12M1/34
CPC分类号： G01N33/48728
摘要： A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.
摘要翻译：用于测量细胞外电位的感测元件，包括衬底，设置在衬底中的阱，设置在阱的壁上的引导部分和形成在衬底的下表面处的检测电极。指导部分是指导药物。井在底部设置有凹陷，并且第一通孔穿过基底的凹陷和下表面。该井用于将主体细胞，培养液和药物混合在一起。上述构成的感测元件精确地测量由被摄体单元产生的变化。

9. 发明授权

US07736477B2 Probe for measuring electric potential of cell 失效
标题翻译：用于测量电池电位的探头
公开(公告)号：US07736477B2
公开(公告)日：2010-06-15
申请号：US10595275
申请日：2005-07-14
申请人： Masaya Nakatani , Nobuhiko Ozaki , Hiroaki Oka
发明人： Masaya Nakatani , Nobuhiko Ozaki , Hiroaki Oka
IPC分类号： G01N33/487
CPC分类号： G01N33/48728
摘要： A probe for measuring an electric potential of a cell includes a plate having a surface having a first cavity provided therein, and a sensor element provided in the first cavity. A second cavity is provided in the bottomsurface of the first cavity. The first flow passage having first and second openings is provided in the plate. The first and second openings of the first flow passage open to the second cavity and outside the plate, respectively. The sensor element includes a thin plate, and a supporting substrate provided around the thin plate and in the first cavity of the plate. The thin plate has a through-hole therein having a first opening and a second opening communicating with the second cavity of the plate. The first flow passage allows fluid to flow therein. A sucking device is coupled with the second opening of the first flow passage as to suck the fluid flowing in the first flow passage. This probe can measure an electric potential of a cell floating in solution as it is in this environment.
摘要翻译：用于测量电池的电位的探针包括具有设置在其中的具有第一腔的表面的板和设置在第一腔中的传感器元件。在第一腔的底面设有第二腔。具有第一和第二开口的第一流动通道设置在板中。第一流动通道的第一和第二开口分别向第二腔室和板外开口。传感器元件包括薄板，以及设置在薄板周围和板的第一腔中的支撑基板。薄板的通孔中具有第一开口和与板的第二腔连通的第二开口。第一流道允许流体流入其中。抽吸装置与第一流动通道的第二开口联接以吸收在第一流动通道中流动的流体。该探头可以在这种环境中测量浮动在溶液中的电池的电位。

10. 发明申请

US20050214740A1 Device for measuring extracellular potential and manufacturing method of the same 审中-公开
标题翻译：用于测量细胞外电位的装置及其制造方法
公开(公告)号：US20050214740A1
公开(公告)日：2005-09-29
申请号：US11081759
申请日：2005-03-17
申请人： Hiroshi Ushio , Masaya Nakatani , Hiroaki Oka , Nobuhiko Ozaki
发明人： Hiroshi Ushio , Masaya Nakatani , Hiroaki Oka , Nobuhiko Ozaki
IPC分类号： G01N33/48 , A61B5/04 , C12M1/00 , C12M1/34 , C12P21/06 , C12Q1/00 , G01N33/487
CPC分类号： G01N33/48728
摘要： A device for measuring extracellular potential includes a diaphragm and a detecting electrode. The diaphragm is formed on one surface thereof with a depression including at least one curved surface, and a hole is provided so as to penetrate from the curved surface of the depression through to the other surface of the diaphragm. An opening on the curved surface of the depression is smaller than an opening on the other side, and a detecting electrode is provided on the wall surface of the hole.
摘要翻译：用于测量细胞外电位的装置包括隔膜和检测电极。隔膜在其一个表面上形成有包括至少一个弯曲表面的凹陷，并且设置有从凹部的弯曲表面穿透到隔膜的另一个表面的孔。凹陷的曲面上的开口小于另一侧的开口，并且在孔的壁面上设置有检测电极。

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