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1. 发明授权

US07285199B2 Apparatus and method for electrophoretic microspot concentration 有权
标题翻译：电泳微球浓度的仪器和方法
公开(公告)号：US07285199B2
公开(公告)日：2007-10-23
申请号：US10399882
申请日：2001-10-31
申请人： Masato Mitsuhashi , Taku Murakami , Tsuruki Tamura , Masafumi Yohda , Mina Okochi
发明人： Masato Mitsuhashi , Taku Murakami , Tsuruki Tamura , Masafumi Yohda , Mina Okochi
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44704 , B01L3/5025 , B01L2300/0618 , B01L2300/0829 , B01L2400/0421 , G01N27/44756 , G01N2001/4016 , G01N2001/4038
摘要： A method and apparatus for concentrating dilute biomolecules in samples using electrophoresis are described. The method involves using the charged nature of the biomolecules to localize them to a microspot containing a dialysis membrane. The method and apparatus may also be used for identifying the presence of a biomolecule in a mixture or to quantitate the amount of a specific biomolecule in a mixture. The method and apparatus may also be used to produce a high throughput method for analysis and quantitation of a sample containing biomolecules.
摘要翻译：描述了使用电泳浓缩样品中的稀释生物分子的方法和装置。该方法包括使用生物分子的带电性质将其定位于含有透析膜的微管。该方法和装置也可用于鉴定混合物中生物分子的存在或定量混合物中特定生物分子的量。该方法和装置还可用于产生用于分析和定量含有生物分子的样品的高通量方法。

2. 发明授权

US10542745B2 Portable device for ex vivo stimulation of whole blood 有权
公开(公告)号：US10542745B2
公开(公告)日：2020-01-28
申请号：US13883269
申请日：2011-10-31
申请人： Masato Mitsuhashi , Taku Murakami
发明人： Masato Mitsuhashi , Taku Murakami
IPC分类号： A01N1/02 , A61B5/15
摘要： Disclosed are methods, device kits, and systems for improved quantification of mRNA from whole blood. More particularly, the devices and kites related thereto are useful for the controlled and repeatable ex vivo stimulation of whole blood.

3. 发明申请

US20130226032A1 PORTABLE DEVICE FOR EX VIVO STIMULATION OF WHOLE BLOOD 审中-公开
标题翻译：便携式装置用于全血淋巴细胞浸润
公开(公告)号：US20130226032A1
公开(公告)日：2013-08-29
申请号：US13883269
申请日：2011-10-31
申请人： Masato Mitsuhashi , Taku Murakami
发明人： Masato Mitsuhashi , Taku Murakami
IPC分类号： A01N1/02 , A61B5/15
CPC分类号： A01N1/0284 , A01N1/0252 , A61B5/15003 , A61B5/150206 , A61B5/150305 , A61B5/150389 , A61B5/150755 , A61B5/153 , A61B5/154 , A61K35/14
摘要： Disclosed are methods, device kits, and systems for improved quantification of mRNA from whole blood. More particularly, the devices and kites related thereto are useful for the controlled and repeatable ex vivo stimulation of whole blood.
摘要翻译：公开了用于改进来自全血的mRNA定量的方法，装置试剂盒和系统。更具体地，与其相关的装置和风筝对于全血的受控和可重复的离体刺激是有用的。

4. 发明申请

US20110045470A1 PATHOGEN DETECTION IN LARGE-VOLUME PARTICULATE SAMPLES 审中-公开
标题翻译：大容量样品中的病原体检测
公开(公告)号：US20110045470A1
公开(公告)日：2011-02-24
申请号：US12671675
申请日：2008-08-01
申请人： Taku Murakami , Jifan Li , Masato Mitsuhashi , Melanie Oakes
发明人： Taku Murakami , Jifan Li , Masato Mitsuhashi , Melanie Oakes
IPC分类号： C12Q1/68 , C12Q1/04 , C12N1/02 , C12M1/34 , G01N33/53
CPC分类号： C12Q1/04 , C12Q1/24
摘要： Methods and filter systems for detecting microorganisms in a sample, such as a food sample, are disclosed. The filter is configured to attract the microorganism, for example by electrostatic charge. The filter containing the microorganism is incubated to grow the microorganism so that it may be detected. The filter may be degradable and the detection may involve extracting the microorganism or the molecular marker of the microorganism from the filter for detection. The filter system may also include a porous microbead component selected to trap dirt and other contaminants from the sample while allowing the microorganisms to pass among the microbeads. Methods are disclosed for detecting microorganisms in samples using the filter systems described. The methods may also include adding a polymer and/or a microorganism detection reagent to the filter to localize microorganism growth and prevent evaporation of reagents.
摘要翻译：公开了用于检测样品（例如食品样品）中的微生物的方法和过滤系统。过滤器被配置为吸引微生物，例如通过静电电荷。将含有微生物的过滤器温育以生长微生物，使其可以被检测。过滤器可以是可降解的，并且检测可能涉及从过滤器中提取微生物或微生物的分子标记物进行检测。过滤系统还可以包括多孔微珠组分，其被选择用于从样品中捕获污垢和其它污染物，同时允许微生物在微珠之间通过。公开了使用所述过滤系统检测样品中的微生物的方法。所述方法还可以包括向过滤器中加入聚合物和/或微生物检测试剂以定位微生物生长并防止试剂的蒸发。

5. 发明授权

US09150920B2 Methods of characterizing host responsiveness to interferon by ex vivo induction of interferon-responsive markers 有权
标题翻译：通过体外诱导干扰素反应标记表征宿主对干扰素的反应性的方法
公开(公告)号：US09150920B2
公开(公告)日：2015-10-06
申请号：US13695755
申请日：2011-05-03
申请人： Masato Mitsuhashi , Ernest C. Borden
发明人： Masato Mitsuhashi , Ernest C. Borden
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6876 , C12Q1/6883 , C12Q1/6886 , C12Q2600/106 , C12Q2600/158
摘要： Embodiments of the invention relate generally to ex vivo methods of quantifying expression of interferon responsive genes and characterizing an individual's potential responsiveness to interferon administration. Certain embodiments relate to methods to monitor the efficacy of ongoing interferon therapy by evaluating expression of interferon responsive genes before and after interferon administration.
摘要翻译：本发明的实施方案一般涉及定量干扰素应答基因的表达的离体方法，并表征个体对干扰素施用的潜在反应性。某些实施方案涉及通过评估干扰素施用前后的干扰素应答基因的表达来监测正在进行的干扰素疗法的功效的方法。

6. 发明授权

US08783810B2 Dot formation positioning device, recording method, setting method, and recording program 有权
标题翻译：点阵定位装置，记录方法，设定方法和记录程序
公开(公告)号：US08783810B2
公开(公告)日：2014-07-22
申请号：US13411092
申请日：2012-03-02
申请人： Tomohiro Yuda , Masato Mitsuhashi , Sumito Anzai
发明人： Tomohiro Yuda , Masato Mitsuhashi , Sumito Anzai
IPC分类号： B41J29/38 , B41J19/14 , B41J2/21
CPC分类号： B41J19/142 , B41J2/2132
摘要： To improve positioning accuracy in a direction of relative movement of dots which are formed by a fluid ejection device in which a plurality of nozzle groups contained in the direction of the relative movement of the head section contain a plurality of nozzles in the direction of the relative movement, an ejection timing is set for adjusting in a direction of the relative movement the positions of dots formed by each of nozzle groups. A distribution of adjustment pixels is set for adjusting in the direction of the relative movement the positions of dots formed by each of nozzles. A fluid is ejected in accordance with raster data generated on the basis of a distribution of the adjustment pixels set by an adjustment pixel setting unit, on the basis of ejection timing set by a nozzle group timing setting unit.
摘要翻译：为了提高由流体喷射装置形成的点的相对运动方向上的定位精度，其中包含在头部相对运动方向上的多个喷嘴组在相对方向上包含多个喷嘴设置喷射时刻，以便在相对运动的方向上调整由每个喷嘴组形成的点的位置。设置调整像素的分布，以便在相对运动的方向上调整由每个喷嘴形成的点的位置。根据由调整像素设定单元设定的调整像素的分布而生成的光栅数据，基于由喷嘴群定时设定单元设定的喷射时刻喷射流体。

7. 发明授权

US08708589B2 Transport device, recording apparatus, transport method 有权
标题翻译：运输装置，记录装置，运输方式
公开(公告)号：US08708589B2
公开(公告)日：2014-04-29
申请号：US13216994
申请日：2011-08-24
申请人： Masato Mitsuhashi , Sumito Anzai
发明人： Masato Mitsuhashi , Sumito Anzai
IPC分类号： B41J11/66 , B41J11/70
CPC分类号： B65H35/04 , B65H29/20 , B65H2404/1441 , B65H2601/421 , Y10T83/04 , Y10T83/4592
摘要： The transport control section controls, at the time of cutting of the long sheet by the cutting unit, the motor for pinching such that a downstream portion in the transport direction, which is cut from the sheet, remains pinched by the first roller and the second roller at the point of time of completion of cutting, and controls, after the completion of cutting, the motor for discharge such that the downstream portion is discharged downstream in the transport direction by rotation of the respective rollers which pinch the downstream portion.
摘要翻译：运输控制部分在通过切割单元切割长片材时控制用于夹持的电动机，使得从片材切割的输送方向上的下游部分保持被第一辊挤压，而第二辊在完成切割的时间点之后，控制用于排出的电动机，使得下游部分通过夹持下游部分的各个辊的旋转沿输送方向向下游排出。

8. 发明授权

US08268982B2 Primers and probes for the detection of HIV 有权
标题翻译：用于检测HIV的引物和探针
公开(公告)号：US08268982B2
公开(公告)日：2012-09-18
申请号：US10585393
申请日：2005-01-07
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： C07H21/04 , C12Q1/68 , C12P19/34 , C12N9/00
CPC分类号： C12Q1/686 , C12Q2561/101
摘要： Provided herein are primer/probe sets useful for detecting HIV (HIV-1) in a test sample. The primer/probe sets can be employed according to nucleic acid amplification procedures including PCR, real-time quantitative PCR, or RT-PCR. The primer/probe sets can also be provided in the form of a kit with other reagents for performing a nucleic acid amplification reaction.
摘要翻译：本文提供了可用于检测测试样品中HIV（HIV-1）的引物/探针组。可以根据包括PCR，实时定量PCR或RT-PCR的核酸扩增方法使用引物/探针组。引物/探针组还可以以试剂盒的形式提供用于进行核酸扩增反应的其它试剂。

9. 发明申请

US20120174653A1 PM EMISSION AMOUNT ESTIMATION DEVICE FOR DIESEL ENGINE 有权
公开(公告)号：US20120174653A1
公开(公告)日：2012-07-12
申请号：US13386142
申请日：2010-06-11
申请人： Yoshikatsu Ikawa , Masato Mitsuhashi , Kazunari Ide
发明人： Yoshikatsu Ikawa , Masato Mitsuhashi , Kazunari Ide
IPC分类号： F01N11/00
CPC分类号： F01N9/002 , F01N3/021 , F01N2900/1606 , F02D41/1456 , F02D41/1467 , F02D2200/0812 , Y02T10/20 , Y02T10/47
摘要： Providing a PM emission amount estimation device for the diesel engine wherein the accuracy of the correction in correcting the basic level of the PM emission amount by use of the PM emission amount estimation map can be enhanced; and, the PM emission amount particularly during the transient state of the engine operation condition can be accurately computed so as to estimate, with high precision, the PM emission amount and the PM emission accumulated-amount (integrated amount) during the whole engine operation conditions including the transient state as well as the steady state. A PM emission amount estimation device for estimating the amount of the PM for estimating the amount of the PM that is emitted from a diesel engine and collected by a DPF provided in the exhaust gas passage of the engine, the device including, but not limited to: a PM emission amount estimation base-map 3 with which the base PM emission amount is computed in response to the operation condition of the engine; a PM emission amount estimation correction-map 5 that determines a correction factor for correcting the base PM emission amount computed by the PM emission amount estimation base-map 3, the correction factor being determined in response to the transient engine operation condition; a transient state judgment device 7 that judges that the engine is operated in a transient operation condition based on the change of the air excess ratio regarding the engine; a PM emission amount computation device 9 that corrects the computed base PM emission amount by multiplying the computed base PM emission amount by the correction factor computed with the PM emission amount estimation correction-map 5 only in a case where it is judged, by the transient state judgment device 7, that the engine is in a transient operation condition whereas the base PM emission amount is directly outputted in a case where it is judged that the engine is in a steady operation condition.

10. 发明申请

US20120132103A1 FLUORINE-CONTAINING COMPOUND, FLUORINE-CONTAINING SURFACTANT AND COMPOSITIONS CONTAINING SAME 有权
标题翻译：含氟化合物，含荧光体的表面活性剂及其组合物
公开(公告)号：US20120132103A1
公开(公告)日：2012-05-31
申请号：US13388195
申请日：2010-07-26
申请人： Katsuyuki Tsugita , Masato Mitsuhashi
发明人： Katsuyuki Tsugita , Masato Mitsuhashi
IPC分类号： C09G1/04 , C07C309/14
CPC分类号： C07C309/14 , C07C305/04 , C09G1/14
摘要： A fluorine-containing compound exhibiting excellent surface tension-reducing ability despite the absence of perfluoroalkyl group having a chain length of 8 or more which had been the cause of the PFOS and PFOA problems and use of a fluorine material with low environmental load is provided. Also provided are a fluorine-containing surfactant and a composition thereof, an aqueous resin emulsion and a floor polish composition containing such surfactant. The fluorine-containing compound is represented by the following formula (1): Rf1—CpH2p—CH(OH)—CqH2q—NR—CrH2r—(O)n—SO3M (1) wherein Rf1 is a C1-6 perfluoroalkyl group, p, q, and r are independently an integer of 1 to 6, M is a cationic atom or atomic group, n is 0 or 1, R is hydrogen atom, a C1-12 alkyl group, or a group represented by the following formula (2): Rf2—CsH2s—CH(OH)—CtH2t— (2) wherein Rf2 is a C1-6 perfluoroalkyl group, and s and t are independently an integer of 1 to 6.
摘要翻译：提供了尽管不具有全氟烷基的链长为8或更大的全氟烷基，其具有优异的表面张力降低能力的含氟化合物，这是PFOS和PFOA问题的原因，以及使用环境负荷低的氟材料。还提供了含氟表面活性剂及其组合物，水性树脂乳液和含有这种表面活性剂的地板油漆组合物。含氟化合物由下式（1）表示：Rf1-CpH2p-CH（OH）-CqH2q-NR-CrH2r-（O）n-SO3M（1）其中Rf1为C1-6全氟烷基，p ，q和r分别为1〜6的整数，M为阳离子性原子或原子团，n为0或1，R为氢原子，碳原子数为1〜12的烷基或下述式 2）：Rf2-CsH2s-CH（OH）-CtH2t-（2）其中Rf2为C1-6全氟烷基，s和t独立地为1〜6的整数。

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