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1. 发明授权

US5610036A Mutant AOX2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism 失效
标题翻译：突变体AOX2启动子，携带其的微生物，其制备方法和使用这种微生物产生异源蛋白质
公开(公告)号：US5610036A
公开(公告)日：1997-03-11
申请号：US288899
申请日：1994-08-10
申请人： Masami Miura , Yutaka Ishida , Hideyuki Oi , Koji Murakami , Yukimitsu Nakagawa , Haruhide Kawabe
发明人： Masami Miura , Yutaka Ishida , Hideyuki Oi , Koji Murakami , Yukimitsu Nakagawa , Haruhide Kawabe
IPC分类号： C07K14/765 , C12N9/26 , C12N15/14 , C12N15/81 , C07H21/04 , C12N1/16 , C12N1/19 , C12P21/02
CPC分类号： C12N9/2408 , C07K14/765 , C12N15/815
摘要： A mutant AOX2 (alcohol oxidase 2) promoter derived from the natural AOX2 promoter by base sequence deletion, insertion, or substitution is disclosed. A microbial strain carrying a gene under the control of such a mutant AOX2 promoter can be obtained by growing in a methanol-containing medium a strain incapable of producing AOX encoded by the AOX1 gene but carrying the AOX2 gene under the control of the natural AOX2 promoter. A heterologous protein may be produced by cultivating the mutant strain with the desired heterologous protein gene incorporated downstream from the mutant AOX2 promoter. A plasmid carrying the mutant AOX2 promoter is also provided.
摘要翻译：公开了通过碱基序列缺失，插入或取代衍生自天然AOX2启动子的突变体AOX2（醇氧化酶2）启动子。携带这种突变体AOX2启动子控制下的基因的微生物菌株可以通过在含甲醇培养基中生长不能产生由AOX1基因编码的AOX但携带AOX2基因在天然AOX2启动子控制下的菌株。可以通过用突变AOX2启动子下游掺入的所需异源蛋白质基因培养突变菌株来产生异源蛋白质。还提供携带突变型AOX2启动子的质粒。

2. 发明授权

US5756313A Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin 失效
标题翻译：含有白蛋白基因的质粒，携带相同的转化体，生产这种转化体和产生白蛋白
公开(公告)号：US5756313A
公开(公告)日：1998-05-26
申请号：US138384
申请日：1993-10-20
申请人： Ken Okabayashi , Hideyuki Oi , Kazumoto Hirabayashi , Masami Miura , Miho Shimizu , Haruhide Kawabe
发明人： Ken Okabayashi , Hideyuki Oi , Kazumoto Hirabayashi , Masami Miura , Miho Shimizu , Haruhide Kawabe
IPC分类号： C12N1/19 , C07K14/765 , C12N9/26 , C12N15/09 , C12N15/14 , C12N15/81 , C12N15/90 , C12P21/00 , C12P21/02 , C12R1/865 , C12N15/00
CPC分类号： C07K14/765 , C12N15/81 , C12N15/90 , C12N9/2408 , C07K2319/02
摘要： A plasmid for a yeast host, the plasmid comprising in sequence: (1) a yeast derived promoter, (2) an albumin-encoding region placed under control of the yeast-derived promoter, (3) a transcription terminator and (4) a sequence homologous to a part of the yeast host chromosomal sequence such that the plasmid is capable of being integrated into the yeast host cell chromosome and wherein the plasmid is incapable of autonomous replication in yeast cells; a yeast host transformed with the above plasmid; a method of producing a yeast transformant which comprises transforming a yeast host by integrating at least two different plasmids each having a sequence homologous to a part of the host yeast chromosomal sequence one at a time into the chromosome of the yeast host cell; and a method of producing albumin which comprises cultivating the above transformant and recovering the thus-produced albumin.
摘要翻译：一种用于酵母宿主的质粒，所述质粒按顺序包含：（1）酵母衍生的启动子，（2）置于酵母衍生的启动子控制下的白蛋白编码区，（3）转录终止子和（4）序列与酵母宿主染色体序列的一部分同源，使得质粒能够整合到酵母宿主细胞染色体中，并且其中质粒不能在酵母细胞中自主复制; 用上述质粒转化的酵母宿主; 生产酵母转化体的方法，其包括通过将至少两种与宿主酵母染色体部分的序列同源的不同质粒整合到酵母宿主细胞的染色体中来转化酵母宿主; 以及生产白蛋白的方法，其包括培养上述转化体并回收由此产生的白蛋白。

3. 发明授权

US07939081B2 Method for producing cercosporamide 失效
标题翻译：铈孢子胺的制备方法
公开(公告)号：US07939081B2
公开(公告)日：2011-05-10
申请号：US11997112
申请日：2006-08-08
申请人： Tsuyoshi Hosoya , Jun Ohsumi , Kiyoshi Hamano , Yasunori Ono , Masami Miura
发明人： Tsuyoshi Hosoya , Jun Ohsumi , Kiyoshi Hamano , Yasunori Ono , Masami Miura
IPC分类号： A61K36/84 , C12P21/04 , A01N63/04
CPC分类号： C07D307/91 , C12P17/04 , C12R1/645
摘要： The present invention provides a process for producing cercosporamide comprising culturing a fungus belonging to the genus Lachnum and/or fungus belonging to the genus Pseudaegerita and recovering cercosporamide from the culture thereof. In addition, the present invention provides cercosporamide acquired according to this production process. Further, the present invention provides novel microorganisms consisting of Lachnum fuscescens SANK 19096, Lachnum calycioides SANK 12497, Lachnum caesaliatum SANK 10906, and Pseudaegerita websteri SANK 11006.
摘要翻译：本发明提供了一种生产铈孢子酰胺的方法，其包括培养属于属于假单胞菌属的Lachnum属和/或真菌的真菌并从其培养物中回收孢子虫酰胺。此外，本发明提供根据该制造方法获得的孢霉素。此外，本发明提供了由Lachnum fuscescens SANK 19096，Lachnum calycioides SANK 12497，Lachnum caesaliatum SANK 10906和Pseudaegerita websterica SANK 11006组成的新型微生物。

4. 发明授权

US07872111B2 Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby 有权
标题翻译：生产蛋白质的方法是由此产生的酸性糖链和糖蛋白的还原
公开(公告)号：US07872111B2
公开(公告)日：2011-01-18
申请号：US12184334
申请日：2008-08-01
申请人： Masami Miura , Masaaki Hirose , Taeko Miwa , Hiroyuki Irie , Shinobu Kuwae , Kenmi Miyano , Wataru Otani , Hideyuki Ohi
发明人： Masami Miura , Masaaki Hirose , Taeko Miwa , Hiroyuki Irie , Shinobu Kuwae , Kenmi Miyano , Wataru Otani , Hideyuki Ohi
IPC分类号： C07H21/04 , C12N1/00 , C12N14/39 , C12N9/10
CPC分类号： C12N9/1205 , C12P21/005
摘要： A protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus Pichia; a gene coding this protein; a mutant of this gene; a vector carrying the mutant gene; a yeast strain belonging to the genus Pichia having been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced, are described.
摘要翻译：参与将磷酸甘露糖添加到源自毕赤酵母属的酵母的糖链的糖链上的蛋白质; 编码该蛋白的基因; 该基因的突变体; 携带突变基因的载体; 已经通过该载体转化了属于毕赤酵母属的酵母菌株; 通过使用转化的酵母菌株来生产具有降低酸性糖链的蛋白质的方法; 和如此制备的糖蛋白。

5. 发明申请

US20100227115A1 Method of Molding Composite Material Structural Member and Composite Material Structural Member 审中-公开
标题翻译：复合材料结构件和复合材料结构件成型方法
公开(公告)号：US20100227115A1
公开(公告)日：2010-09-09
申请号：US12223068
申请日：2007-04-27
申请人： Kouji Esaki , Yoshinori Nonaka , Masami Miura , Shigeru Nishiyama , Toshio Abe
发明人： Kouji Esaki , Yoshinori Nonaka , Masami Miura , Shigeru Nishiyama , Toshio Abe
IPC分类号： B32B5/12 , B32B37/26
CPC分类号： B29C70/34 , B29C70/222 , B29C70/342 , B29D99/0003 , B29K2105/246 , B29L2031/003 , B29L2031/3076 , Y10T156/1067 , Y10T428/24132
摘要： The present invention relates to a method of molding a composite material structural member used mainly within structural members such as channel materials or angle materials, and also to a composite material structural member. An object of the invention is to provide a method of molding a composite material structural member that is capable of suppressing fiber creasing even for very long shapes having non-developable surfaces, and also to provide a composite material structural member.When pressing a prepreg laminate against a molding die, the method comprises a preparation step of preparing a prepreg laminate for molding by laminating a plurality of prepregs with different fiber orientations into a flat plate shape, and a pressure application step of pressing the prepreg laminate for molding prepared in the preparation step against the molding die, and in the preparation step, a specified prepreg having a fiber orientation that coincides with, or is close to, the direction of creasing occurrence is split, either within the region of creasing occurrence or in the vicinity thereof, along a direction that is effective in inhibiting creasing occurrence, and the split prepreg is then used in preparing the prepreg laminate.
摘要翻译：本发明涉及一种主要用于诸如通道材料或角材料的结构构件中的复合材料结构构件的成型方法，以及复合材料结构构件。本发明的目的是提供一种即使对于具有不可展开表面的非常长的形状也能够抑制纤维皱纹的复合材料结构件的成型方法，并且还提供复合材料结构构件。当将预浸料层合体压在成形模上时，该方法包括通过将具有不同纤维取向的多个预浸料层压成平板状而制备用于成型的预浸料层合体的制备步骤，以及将预浸料层压板在制备步骤中制备的模塑成型模具，并且在制备步骤中，具有与折痕发生方向一致或接近于折痕发生方向的纤维取向的特定预浸料被分裂，或者在折皱区域内或在折痕区域内其附近沿着有效抑制折痕发生的方向，然后将该预浸料坯用于制备预浸料层压体。

6. 外观设计

USD443475S1 Cup for beverages or food 失效
公开(公告)号：USD443475S1
公开(公告)日：2001-06-12
申请号：US29112047
申请日：1999-10-07
申请人： Masami Miura
设计人： Masami Miura

7. 发明授权

US09219381B2 Charging system, control method thereof, and program 有权
标题翻译：充电系统及其控制方法和程序
公开(公告)号：US09219381B2
公开(公告)日：2015-12-22
申请号：US13389195
申请日：2011-02-25
申请人： Mitsufumi Goto , Masami Miura
发明人： Mitsufumi Goto , Masami Miura
IPC分类号： H02J7/00 , H01M10/44 , H01M10/46 , H02J1/00 , H02J3/00 , G01N27/416 , H02J7/35 , H02J7/34 , H02J3/38
CPC分类号： H02J7/35 , H01M10/441 , H01M10/46 , H01M10/465 , H02J3/383 , H02J3/386 , H02J7/34 , H02J2007/0095 , Y02E10/563 , Y02E10/566 , Y02E10/763 , Y02E10/766
摘要： A power generating unit generates electric power using natural energy, plural secondary battery packs are charged with the electric power generated by the power generating unit, and the secondary battery pack as a power source to be used for an operation control of a control unit controlling the operation of a charging system is selected on the basis of a state of charge of the corresponding secondary battery pack.
摘要翻译：发电单元利用自然能产生电力，多个二次电池组被发电单元产生的电力充电，二次电池组作为电源用于控制单元的控制单元的运行控制基于对应的二次电池组的充电状态来选择充电系统的操作。

8. 发明申请

US20120148890A1 BATTERY SYSTEM 审中-公开
标题翻译：电池系统
公开(公告)号：US20120148890A1
公开(公告)日：2012-06-14
申请号：US13390605
申请日：2011-03-15
申请人： Mitsufumi Goto , Masami Miura
发明人： Mitsufumi Goto , Masami Miura
IPC分类号： H01M10/48
CPC分类号： H01M2/1077 , H01M2/345 , H01M10/425 , H01M10/482 , H01M10/486 , H01M2010/4271
摘要： A battery system (1) includes: an electrical cell (2) which is accommodated in each battery accommodation casing (22) so that at least one of first facing side surfaces (51) and (52) in a stacking direction (X) of a stacked structure and at least one of second facing side surfaces (53) and (54) in the direction (Y) lying at right angles to the stacking direction (X) faces the battery accommodation casing wall surface (22a) or the battery accommodation casing partition plate wall surface (23a); separation state detecting devices (24a) and (24b) for detecting a first separation distance (W1) between the first side surfaces (51) and (52) and any one of the wall surfaces (22a) and (23a) and a second separation distance (W2) between the second side surfaces (53) and (54) and any one of the wall surfaces (22a) and (23a); and a control unit that determines that the corresponding secondary battery (2) has an abnormal internal pressure when both the first separation distance (W1) and the second separation distance (W2) become smaller on the basis of the detection result obtained by the separation state detecting devices (24a) and (24b).
摘要翻译：电池系统（1）包括：容纳在每个电池容纳壳体（22）中的电池（2），使得在层叠方向（X）上的第一面对侧表面（51）和（52）中的至少一个与层叠方向（X）成直角的方向（Y）上的堆叠结构和第二面侧面（53）和（54）中的至少一个面向电池容纳壳体壁面（22a）或电池容纳套管隔板墙面（23a）; 用于检测第一侧表面（51）和（52）与任何一个壁表面（22a）和（23a）之间的第一分隔距离（W1）的分离状态检测装置（24a）和（24b）第二侧面（53）和（54）之间的距离（W2）和壁面（22a）和（23a）中的任何一个之间的距离（W2）。以及控制单元，其基于通过分离状态获得的检测结果，当第一间隔距离（W1）和第二间隔距离（W2）都变小时，确定相应的二次电池（2）具有异常的内部压力检测装置（24a）和（24b）。

9. 发明授权

US5707827A Mutant AOX2 promoter, vector carrying same, transformant and production of heterologous protein 失效
标题翻译：突变体AOX2启动子，携带相同载体，转化体和产生异源蛋白质
公开(公告)号：US5707827A
公开(公告)日：1998-01-13
申请号：US281025
申请日：1994-07-27
申请人： Hideyuki Ohi , Masami Miura , Ryuji Hiramatsu , Takao Ohmura
发明人： Hideyuki Ohi , Masami Miura , Ryuji Hiramatsu , Takao Ohmura
IPC分类号： C12N1/19 , C07K14/765 , C12N15/09 , C12N15/81 , C12P21/02 , C12R1/84 , C07H21/04 , C12N15/67
CPC分类号： C12N15/815 , C07K14/765
摘要： A mutant AOX2 promoter wherein 1 to 3 oligonucleotide(s) GATAGGCTATTTTTGTCGCATAAAT (SEQUENCE ID NO: 2) is (are) added in the normal direction, the reverse direction or in both the normal and reverse directions at the 5' end side of a partial DNA fragment of a wild-type AOX2 promoter, a vector carrying the promoter, a transformant into which the vector has been introduced and a method for producing a heterologous protein, comprising culture of the transformant. The mutant AOX2 promoter of the present invention has a markedly enforced promoter activity as compared with wild-type AOX2 promoters. Accordingly, the promoter of the present invention is highly utilizable as a promoter to be carried by a vector capable of expressing a heterologous protein. The vector of the present invention can efficiently express and produce various useful heterologous proteins in hosts.
摘要翻译：在一部分的5'末端侧沿正常方向，相反方向或正向和反向两个方向添加1至3个寡核苷酸GATAGGCTATTTTTGTCGCATAAAT（SEQ ID NO：2）的突变体AOX2启动子野生型AOX2启动子的DNA片段，携带启动子的载体，导入载体的转化体和异源蛋白质的制备方法，包括转化体的培养。与野生型AOX2启动子相比，本发明的突变型AOX2启动子具有明显的促进启动子活性。因此，本发明的启动子高度可用作能够表达异源蛋白质的载体携带的启动子。本发明的载体可以有效地在宿主中表达和产生各种有用的异源蛋白质。

10. 发明授权

US4674121A Circuit for detecting the level of noise in FM signal for use in AM/FM receiver 失效
标题翻译：用于检测AM / FM接收机中使用的FM信号中的噪声电平的电路
公开(公告)号：US4674121A
公开(公告)日：1987-06-16
申请号：US777163
申请日：1985-09-18
申请人： Masami Miura , Naotoshi Higashiyama
发明人： Masami Miura , Naotoshi Higashiyama
IPC分类号： H03D5/00 , H04B1/16 , H04H5/00
CPC分类号： H04B1/1661 , H03D5/00
摘要： A circuit is provided for detecting the level of noises in received FM signals in an AM/FM receiver which includes at least an FM intermediate-frequency amplifier, an FM detector connected thereto, an AM intermediate-frequency amplifier and an AM detector connected thereto. This FM noise level detecting circuit includes a frequency converter receiving the output of the FM detector for converting noise components contained in the output of the FM detector into a noise signal of the AM intermediate-frequency, which is in turn outputted to the AM intermediate-frequency amplifier, so that the converted noise signal is amplified by the AM intermediate-frequency amplifier and then detected by the AM detector to produce a DC signal. The signal thus obtained is not only representative of the level of the noise contained in the received FM signal, but also representative of the received FM signal strength.
摘要翻译：提供了一种用于检测AM / FM接收机中接收的FM信号中的噪声电平的电路，其包括至少一个FM中频放大器，与其连接的FM检测器，AM中频放大器和与其连接的AM检测器。该FM噪声电平检测电路包括频率转换器，接收FM检测器的输出，用于将FM检波器的输出中包含的噪声分量转换成AM中频的噪声信号，该噪声信号又被输出到AM中间 - 使得转换的噪声信号由AM中频放大器放大，然后由AM检测器检测以产生DC信号。这样获得的信号不仅代表接收的FM信号中包含的噪声电平，而且也代表接收到的FM信号强度。

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