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1. 发明授权

US06462179B1 Phenyldiboronic acid reagents and complexes 失效
标题翻译：苯二硼酸试剂和络合物
公开(公告)号：US06462179B1
公开(公告)日：2002-10-08
申请号：US09625231
申请日：2000-07-25
申请人： Mark L. Stolowitz , Edward A. Kesicki , Kevin P. Lund , Karin A. Hughes
发明人： Mark L. Stolowitz , Edward A. Kesicki , Kevin P. Lund , Karin A. Hughes
IPC分类号： C07F502
CPC分类号： C07C233/49 , C07C229/38 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/025 , C07K5/0207 , G01N33/531 , G01N33/54353 , Y10S530/81 , Y10S530/816
摘要： Reagents suitable for the modification of a bioactive species for the purpose of incorporating one or more phenyldiboronic acid (PDBA) moieties for subsequent conjugation to a different (or the same) bioactive species having one or more pendant boronic compound complexing moieties of the general formula of General Formula I, wherein group R is a reactive electrophilic or nucleophilic moiety suitable for reaction of the PDBA reagent with a bioactive species. Group Z is a spacer selected from a saturated or unsaturated chain up to about 0 to 6 carbon equivalents in length, an unbranched saturated or unsaturated chain of from about 6 to 18 carbon equivalents in length with at least one intermediate amide or disulfide moiety, and a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length. Group Q is a linkage that includes one of amide, ether and thioether moieties. Group R is preferably selected from, but not limited to, one of acrylamide, bromo, bromoacetamide, chloro, chloroacetamide, dithiopyridyl, hydrazide, N-hydroxysuccinimidyl ester, N-hydroxysulfo-succinimidyl ester, imidate ester, imidazolide, iodo, iodoacetamide, maleimide, amino and thiol moieties. Group Z is preferably an unbranched alkyl chain of the general formula (CH2)n, wherein n=1 to 6. Group Q is preferably selected from one of NHCO, CONH, NHCOCH2, CONHCH2, O, OCH2, S, and SCH2 moieties.
摘要翻译：适于修饰生物活性物质的试剂，用于引入一种或多种苯基二硼酸（PDBA）部分以用于随后与不同（或相同）生物活性物质缀合的具有一个或多个通式的侧链化合物络合部分的生物活性物质通式I，其中基团R是适于PDBA试剂与生物活性物质反应的反应性亲电或亲核部分。 Z组是选自饱和或不饱和链长度至多约0至6当量长度的间隔基，长度为约6至18当量长度的至少一个中间体酰胺或二硫化物部分的无支链饱和或不饱和链，以及长度为约3至12碳当量的聚乙二醇链。 Q组是包括酰胺，醚和硫醚部分之一的连接。基团R优选选自但不限于丙烯酰胺，溴代，溴乙酰胺，氯代，氯乙酰胺，二硫代吡啶基，酰肼，N-羟基琥珀酰亚胺酯，N-羟基磺基琥珀酰亚胺酯，亚氨酸酯，咪唑啉，碘，碘乙酰胺，马来酰亚胺，氨基和硫醇部分。组Z优选为通式（CH 2）n的无支链烷基链，其中n = 1-6。组Q优选选自NHCO，CONH，NHCOCH2，CONHCH2，O，OCH2，S和SCH2部分之一。

2. 发明授权

US6124471A Phenyldiboronic acid reagents and complexes 失效
标题翻译：苯二硼酸试剂和络合物
公开(公告)号：US6124471A
公开(公告)日：2000-09-26
申请号：US407673
申请日：1999-09-28
申请人： Mark L. Stolowitz , Edward A. Kesicki , Kevin P. Lund , Karin A. Hughes
发明人： Mark L. Stolowitz , Edward A. Kesicki , Kevin P. Lund , Karin A. Hughes
IPC分类号： C07C229/38 , C07C233/49 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/44 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/02 , G01N33/531 , G01N33/543
CPC分类号： C07C233/49 , C07C229/38 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/025 , C07K5/0207 , G01N33/531 , G01N33/54353 , Y10S530/81 , Y10S530/816
摘要： Reagents suitable for the modification of a bioactive species for the purpose of incorporating one or more phenyldiboronic acid (PDBA) moieties for subsequent conjugation to a different (or the same) bioactive species having one or more pendant boronic compound complexing moieties of the general formula of General Formula I, ##STR1## wherein group R is a reactive electrophilic or nucleophilic moiety suitable for reaction of the PDBA reagent with a bioactive species. Group Z is a spacer selected from a saturated or unsaturated chain up to about 0 to 6 carbon equivalents in length, an unbranched saturated or unsaturated chain of from about 6 to 18 carbon equivalents in length with at least one intermediate amide or disulfide moiety, and a polyethylene glycol chain of from about 3 to 12 carbon equivalents in length. Group Q is a linkage that includes one of amide, ether and thioether moieties. Group R is preferably selected from, but not limited to, one of acrylamide, bromo, bromoacetamide, chloro, chloroacetamide, dithiopyridyl, hydrazide, N-hydroxysuccinimidyl ester, N-hydroxysulfo-succinimidyl ester, imidate ester, imidazolide, iodo, iodoacetamide, maleimide, amino and thiol moieties. Group Z is preferably an unbranched alkyl chain of the general formula (CH.sub.2)n, wherein n=1 to 6. Group Q is preferably selected from one of NHCO, CONH, NHCOCH.sub.2, CONHCH.sub.2, O, OCH.sub.2, S and SCH.sub.2 moieties.
摘要翻译：适于修饰生物活性物质的试剂，用于引入一种或多种苯基二硼酸（PDBA）部分以用于随后与不同（或相同）生物活性物质缀合的具有一个或多个通式的侧链化合物络合部分的生物活性物质通式I，其中基团R是适于PDBA试剂与生物活性物质反应的反应性亲电或亲核部分。 Z组是选自饱和或不饱和链长度至多约0至6当量长度的间隔基，长度为约6至18当量长度的至少一个中间体酰胺或二硫化物部分的无支链饱和或不饱和链，以及长度为约3至12碳当量的聚乙二醇链。 Q组是包括酰胺，醚和硫醚部分之一的连接。基团R优选选自但不限于丙烯酰胺，溴代，溴乙酰胺，氯代，氯乙酰胺，二硫代吡啶基，酰肼，N-羟基琥珀酰亚胺酯，N-羟基磺基琥珀酰亚胺酯，亚氨酸酯，咪唑啉，碘，碘乙酰胺，马来酰亚胺，氨基和硫醇部分。组Z优选为通式（CH 2）n的无支链烷基链，其中n = 1-6。组Q优选选自NHCO，CONH，NHCOCH2，CONHCH2，O，OCH2，S和SCH2部分之一。

3. 发明授权

US6075126A Phenyldiboronic acid reagents and complexes 失效
标题翻译：苯二硼酸试剂和络合物
公开(公告)号：US6075126A
公开(公告)日：2000-06-13
申请号：US138105
申请日：1998-08-21
申请人： Mark L. Stolowitz , Edward A. Kesicki , Kevin P. Lund , Karin A. Hughes
发明人： Mark L. Stolowitz , Edward A. Kesicki , Kevin P. Lund , Karin A. Hughes
IPC分类号： C07C229/38 , C07C233/49 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/44 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/02 , G01N33/531 , G01N33/543 , C07F5/04
CPC分类号： C07C233/49 , C07C229/38 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/025 , C07K5/0207 , G01N33/531 , G01N33/54353 , Y10S530/81 , Y10S530/816
摘要： Boron compound complexing reagents, boron compound complexes, and methods of synthesizing these reagents and complexes are disclosed. These reagents and complexes include those shown in General Formula CIII, General Formula CIV, and General Formula CVI. In one embodiment, the reagents of General Formula CIII may be used to produce, after condensation with a bioactive species (BAS), the reagent of General Formula CIV. The reagent of General Formula CIV may be used to form a complex with a boron compound, such as a complex shown in General Formula CVI. ##STR1##
摘要翻译：硼化合物络合剂，硼化合物络合物，以及合成这些试剂和络合物的方法。这些试剂和配合物包括通式CIII，通式CIV和通式CVI所示的试剂和络合物。在一个实施方案中，通式CIII的试剂可用于在与生物活性物质（BAS）缩合之后产生通式CIV的试剂。通式CIV的试剂可用于与硼化合物形成络合物，例如通式CVI所示的络合物。

4. 发明授权

US06818760B1 Removal of dye-labeled dideoxy terminators from DNA sequencing reactions 失效
标题翻译：从DNA测序反应中去除染料标记的双脱氧终止子
公开(公告)号：US06818760B1
公开(公告)日：2004-11-16
申请号：US09680889
申请日：2000-10-06
申请人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
发明人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
IPC分类号： C07H2102
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q2563/107 , C12Q2563/149 , C12Q2565/137
摘要： This invention provides methods for removing unincorporated dye-labeled molecules from a mixture that includes dye-labeled polynucleotides or other polymers and the unincorporated dye-labeled molecules. The methods involve adsorbing the unincorporated dye-labeled molecules into a plurality of particles that are made up of one or more porous hydrophobic materials that are encapsulated in a hydrophilic matrix.
摘要翻译：本发明提供了从包含染料标记的多核苷酸或其它聚合物的混合物和未掺入的染料标记分子的混合物中除去未结合的染料标记的分子的方法。所述方法包括将未结合的染料标记的分子吸附到多个由包封在亲水基质中的一种或多种多孔疏水性材料构成的颗粒中。

5. 发明授权

US06414136B1 Removal of dye-labeled dideoxy terminators from DNA sequencing reactions 失效
标题翻译：从DNA测序反应中去除染料标记的双脱氧终止子
公开(公告)号：US06414136B1
公开(公告)日：2002-07-02
申请号：US09564117
申请日：2000-05-03
申请人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
发明人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
IPC分类号： C07H2102
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q2563/107 , C12Q2563/149 , C12Q2565/137
摘要： This invention provides methods for removing unincorporated fluorescent dye-labeled molecules from a mixture that includes fluorescently-labeled polynucleotides and the unincorporated fluorescent dye-labeled molecules. The methods involve adsorbing the unincorporated fluorescent dye-labeled molecules into a plurality of particles that are made up of one or more porous hydrophobic materials that are encapsulated in a hydrophilic matrix.
摘要翻译：本发明提供从包含荧光标记的多核苷酸和未掺入的荧光染料标记的分子的混合物中除去未结合的荧光染料标记的分子的方法。所述方法包括将未掺入的荧光染料标记的分子吸附到由封装在亲水基质中的一种或多种多孔疏水性材料构成的多个颗粒中。

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