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1. 发明授权

US09006410B2 Nucleic acid for plant expression of a fusion protein comprising hydroxyproline O-glycosylation glycomodule 有权
标题翻译：用于植物表达包含羟脯氨酸O-糖基化糖基化的融合蛋白的核酸
公开(公告)号：US09006410B2
公开(公告)日：2015-04-14
申请号：US13019819
申请日：2011-02-02
申请人： Marcia J. Kieliszewski , Jianfeng Xu , Gary D. Meyer
发明人： Marcia J. Kieliszewski , Jianfeng Xu , Gary D. Meyer
IPC分类号： C12N15/82 , C12N15/18 , C12N15/20 , C12N15/62 , C07H21/04 , C07K14/415 , C07K14/61 , C07K14/765 , C12P21/00 , C07K14/56 , A61K38/21 , A61K38/27
CPC分类号： C07K14/415 , A61K38/27 , C07K14/56 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/8257 , C12P21/005
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.
摘要翻译：包括使用驱动植物中翻译后修饰的密码子的蛋白质工程化糖基化位点的重组蛋白质的植物表达产量增加到克隆基因或cDNA的方法，并且设计克隆的基因或cDNA以包含靶向基因的植物分泌信号序列存在用于分泌的产品（蛋白质）。该方法导致重组糖基化蛋白质产量增加。也存在根据这些方法生产的蛋白质。

2. 发明申请

US20110230404A1 Glycoproteins Produced in Plants and Methods of Their Use 审中-公开
标题翻译：在植物中生产的糖蛋白及其使用方法
公开(公告)号：US20110230404A1
公开(公告)日：2011-09-22
申请号：US13005715
申请日：2011-01-13
申请人： Marcia J. Kieliszewski , Jianfeng Xu , John Kopchick , Shigeru Okada , Gary D. Meyer
发明人： Marcia J. Kieliszewski , Jianfeng Xu , John Kopchick , Shigeru Okada , Gary D. Meyer
IPC分类号： A61K38/27 , A61P3/10 , C07K14/61 , A61P5/06
CPC分类号： C07K14/415 , A61K38/27 , C07K14/56 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/8257 , C12P21/005
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.
摘要翻译：包括使用驱动植物翻译后修饰的密码子将包含工程化糖基化位点的重组蛋白质的产量提高到克隆基因或蛋白质cDNA的方法; 并对克隆的基因或cDNA进行工程以包含靶向分泌基因产物（蛋白质）的植物分泌信号序列。该方法导致重组糖基化蛋白质产量增加。公开了根据这些方法生产的蛋白质。

3. 发明申请

US20110217766A1 Methods of Producing Peptides in Plants and Peptides Produced Thereby 有权
标题翻译：在此产生的植物和肽中产生肽的方法
公开(公告)号：US20110217766A1
公开(公告)日：2011-09-08
申请号：US13019819
申请日：2011-02-02
申请人： Marcia J. Kieliszewski , Jianfeng Xu , Gary D. Meyer
发明人： Marcia J. Kieliszewski , Jianfeng Xu , Gary D. Meyer
IPC分类号： C12N15/85
CPC分类号： C07K14/415 , A61K38/27 , C07K14/56 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/8257 , C12P21/005
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.
摘要翻译：包括使用驱动植物中翻译后修饰的密码子的蛋白质工程化糖基化位点的重组蛋白质的植物表达产量增加到克隆基因或cDNA的方法，并且设计克隆的基因或cDNA以包含靶向基因的植物分泌信号序列存在用于分泌的产品（蛋白质）。该方法导致重组糖基化蛋白质产量增加。也存在根据这些方法生产的蛋白质。

4. 发明申请

US20100029548A1 GLYCOPROTEINS PRODUCED IN PLANTS AND METHODS OF THEIR USE 审中-公开
标题翻译：在植物中生产的GLYCOPROTEINS及其使用方法
公开(公告)号：US20100029548A1
公开(公告)日：2010-02-04
申请号：US12117692
申请日：2008-05-08
申请人： Marcia J. Kieliszewski , Jianfeng Xu , John Kopchick , Shigeru Okada , Gary D. Meyer
发明人： Marcia J. Kieliszewski , Jianfeng Xu , John Kopchick , Shigeru Okada , Gary D. Meyer
IPC分类号： A61K38/14 , C07K14/575 , A61K38/27 , A61P5/00 , A61P3/10
CPC分类号： C07K14/415 , A61K38/27 , C07K14/56 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/8257 , C12P21/005
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.
摘要翻译：提高重组蛋白质植物表达产量的方法，其包括：使用驱动植物翻译后修饰的密码子将糖基化位点工程化为克隆基因或蛋白质cDNA; 并对克隆的基因或cDNA进行工程以包含靶向分泌基因产物（蛋白质）的植物分泌信号序列。该方法导致重组糖基化蛋白质产量增加。公开了根据这些方法生产的蛋白质。

5. 发明申请

US20100028993A1 METHODS OF PRODUCING PEPTIDES IN PLANTS AND PEPTIDES PRODUCED THEREBY 审中-公开
标题翻译：在植物中生产肽的方法和生产的肽
公开(公告)号：US20100028993A1
公开(公告)日：2010-02-04
申请号：US12122606
申请日：2008-05-16
申请人： Marcia J. Kieliszewski , Jianfeng Xu , Gary D. Meyer
发明人： Marcia J. Kieliszewski , Jianfeng Xu , Gary D. Meyer
IPC分类号： C12N15/74
CPC分类号： C07K14/415 , A61K38/27 , C07K14/56 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/8257 , C12P21/005
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present.
摘要翻译：包括使用驱动植物中翻译后修饰的密码子的蛋白质工程化糖基化位点的重组蛋白质的植物表达产量增加到克隆基因或cDNA的方法，并且设计克隆的基因或cDNA以包含靶向基因的植物分泌信号序列存在用于分泌的产品（蛋白质）。该方法导致重组糖基化蛋白质产量增加。也存在根据这些方法生产的蛋白质。

6. 发明申请

US20080242834A1 Methods of Predicting Hyp-Glycosylation Sites For Proteins Expressed and Secreted in Plant Cells, and Related Methods and Products 审中-公开
标题翻译：预测植物细胞表达和分泌的蛋白质的低糖基化位点的方法及相关方法和产品
公开(公告)号：US20080242834A1
公开(公告)日：2008-10-02
申请号：US11995063
申请日：2006-07-10
申请人： Marcia J. Kieliszewski , Jianfeng Xu
发明人： Marcia J. Kieliszewski , Jianfeng Xu
IPC分类号： C07K2/00 , C12P21/00
CPC分类号： C12N15/8257 , G16B30/00
摘要： Proteins with Hyp-glycosylation are more likely to be secreted in plant cells at high levels than those without. Methods are disclosed for the prediction of Pro-hydroxylation and Hyp-glycosylationsites in proteins. Such methods can be used to identify (1) proteins which, without modification, are predisposed to develop Hyp-glycosylation, if expressed in plant cells, and (2) modifications (especially substitution mutations) which increase the propensity of a protein to develop Hyp-glycosylation, with a view to high level or increased secretion. It is also possible to determine empirically whether a particular protein will undergo Hyp-glycosylation suitable for the desired level of secretion in plant cells. Both modified proteins, and methods for the expression and secretion of predisposed and modified proteins, are claimed.
摘要翻译：具有低糖基化功能的蛋白质在植物细胞中比不含有高蛋白质的蛋白质更有可能分泌。公开了用于预测蛋白质中的Pro-羟基化和Hyp-糖基化的方法。这样的方法可用于鉴定（1）如果在植物细胞中表达，则不经修饰，易于发生Hyp-糖基化的蛋白质，和（2）增加蛋白质发展Hyp的倾向的修饰（特别是取代突变）糖基化，以达到高水平或增加分泌。也可以根据经验确定特定蛋白质是否将进行适合植物细胞中所需水平分泌的Hyp糖基化。要求修饰的蛋白质，以及倾向和修饰的蛋白质的表达和分泌的方法。

7. 发明授权

US08563687B2 Synthetic genes for plant gums and other hydroxyproline rich glycoproteins 失效
标题翻译：植物牙龈和其他富含羟脯氨酸的糖蛋白的合成基因
公开(公告)号：US08563687B2
公开(公告)日：2013-10-22
申请号：US12820943
申请日：2010-06-22
申请人： Marcia J. Kieliszewski
发明人： Marcia J. Kieliszewski
IPC分类号： C07K9/00 , C07K14/00 , C07H21/04
CPC分类号： C07K14/415 , C07K2319/00 , C12N15/8241 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabino-galactan proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

8. 发明申请

US20140287457A9 SYNTHETIC GENES FOR PLANT GUMS AND OTHER HYDROXYPROLINE-RICH GLYCOPROTEINS 有权
标题翻译：植物学家和其他羟脯氨酸富含蛋白的合成基因
公开(公告)号：US20140287457A9
公开(公告)日：2014-09-25
申请号：US13149016
申请日：2011-05-31
申请人： Marcia J. Kieliszewski
发明人： Marcia J. Kieliszewski
IPC分类号： C12P21/02 , C12N15/82 , C12N15/11
CPC分类号： C07K14/415 , C07K2319/00 , C12N15/8241 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

9. 发明申请

US20100261874A1 SYNTHETIC GENES FOR PLANT GUMS AND OTHER HYDROXYPROLINE RICH GLYCOPROTEINS 失效
标题翻译：植物学家和其他羟脯氨酸富含蛋白酶的合成基因
公开(公告)号：US20100261874A1
公开(公告)日：2010-10-14
申请号：US12820943
申请日：2010-06-22
申请人： Marcia J. Kieliszewski
发明人： Marcia J. Kieliszewski
IPC分类号： C07K9/00 , C07H21/04 , C07K14/00
CPC分类号： C07K14/415 , C07K2319/00 , C12N15/8241 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabino-galactan proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAUP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAUP）的肽序列）设计的合成基因的表达。

10. 发明授权

US06548642B1 Synthetic genes for plant gums 失效
标题翻译：植物胶的合成基因
公开(公告)号：US06548642B1
公开(公告)日：2003-04-15
申请号：US09119507
申请日：1998-07-20
申请人： Marcia J. Kieliszewski
发明人： Marcia J. Kieliszewski
IPC分类号： C07K700
CPC分类号： C12N15/8241 , C07K14/415 , C07K2319/00 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabino-galactan proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

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