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    • 1. 发明申请
    • Cartridge and System for Manipulating Samples in Liquid Droplets
    • 用于操作液滴样品的墨盒和系统
    • US20130020202A1
    • 2013-01-24
    • US13188584
    • 2011-07-22
    • Marc N. FEIGLINJoseph MamoneAnne R. Kopf-SillIan FitzpatrickMark RobPhillip Duncan
    • Marc N. FEIGLINJoseph MamoneAnne R. Kopf-SillIan FitzpatrickMark RobPhillip Duncan
    • G01N27/447B01D57/02
    • B01L3/502738B01L3/502715B01L3/502784B01L3/5029B01L3/523B01L3/527B01L7/52B01L2200/025B01L2200/027B01L2200/04B01L2200/0647B01L2200/141B01L2300/044B01L2300/0672B01L2300/0681B01L2300/0829B01L2300/0864B01L2300/0867B01L2300/0887B01L2300/089B01L2300/161B01L2400/0427B01L2400/043B01L2400/0478B01L2400/0638B01L2400/0683
    • A cartridge (1) comprises a working film (10) for manipulating samples in liquid droplets with an electrode array (20) when the working film (10) of the cartridge (1) is placed on said electrode array (20). The cartridge (1) comprises a body (2,2′,2″) with a number of wells (5) configured to hold therein reagents (6) or samples (6′); a flexibly deformable top structure (7) impermeable to liquids and configured to seal a top side of the wells (5); a piercable bottom structure (8) impermeable to liquids and configured to seal a bottom side of the wells (5); a working film (10) located below a lower surface (4) of the body (2,2′,2″), the working film (10) being impermeable to liquids and comprising a hydrophobic upper surface (11); a peripheral spacer (9,9′,9″) located below the lower surface (4) of the body (2,2′,2″) and connecting the working film (10) to the body (2,2′,2″); a gap (12) between the lower surface (4) of the body (2,2′,2″) and the hydrophobic upper surface (11) of the working film (10), the gap (12) being defined by the peripheral spacer (9,9′,9″); and a number of piercing elements (13) located below piercable bottom structures (8) and configured to pierce the piercable bottom structures (8) for releasing reagents or samples (6,6′) from the wells (5) into the gap (12). Also disclosed is a system (40) with an electrode array (20) onto which the cartridge (1) can be placed.
    • 当药筒(1)的工作薄膜(10)被放置在所述电极阵列(20)上时,墨盒(1)包括用于用电极阵列(20)操纵液滴样品的工作薄膜(10)。 墨盒(1)包括具有多个孔(5)的主体(2,2',2“),其构造成容纳其中的试剂(6)或样品(6'); 柔性可变形顶部结构(7),其不透液体并且构造成密封所述孔(5)的顶侧; 不透液体并且被构造成密封井(5)的底侧的可穿透底部结构(8); 位于主体(2,2',2“)的下表面(4)下方的工作薄膜(10),所述工作薄膜(10)不透液体并包括疏水性上表面(11); 位于主体(2,2',2“)的下表面(4)下方的外围间隔件(9,9',9”),并将工作膜(10)连接到主体(2,2',2“ “); 主体(2,2',2“)的下表面(4)与工作薄膜(10)的疏水性上表面(11)之间的间隙(12),间隙(12)由周边 间隔物(9,9',9“); 以及位于穿孔底部结构(8)下面的多个穿孔元件(13),并被构造成刺穿可穿透底部结构(8),用于将试剂或样品(6,6')从孔(5)释放到间隙(12) )。 还公开了一种具有电极阵列(20)的系统(40),其上可以放置盒(1)。
    • 2. 发明授权
    • Cartridge and system for manipulating samples in liquid droplets
    • 用于操作液滴样品的墨盒和系统
    • US08470153B2
    • 2013-06-25
    • US13188584
    • 2011-07-22
    • Marc N. FeiglinJoseph MamoneAnne R. Kopf-SillIan FitzpatrickMark RobPhillip Duncan
    • Marc N. FeiglinJoseph MamoneAnne R. Kopf-SillIan FitzpatrickMark RobPhillip Duncan
    • C02F1/04C02F11/00C25B9/00C25B11/00C25B13/00G01N27/00
    • B01L3/502738B01L3/502715B01L3/502784B01L3/5029B01L3/523B01L3/527B01L7/52B01L2200/025B01L2200/027B01L2200/04B01L2200/0647B01L2200/141B01L2300/044B01L2300/0672B01L2300/0681B01L2300/0829B01L2300/0864B01L2300/0867B01L2300/0887B01L2300/089B01L2300/161B01L2400/0427B01L2400/043B01L2400/0478B01L2400/0638B01L2400/0683
    • A cartridge (1) comprises a working film (10) for manipulating samples in liquid droplets with an electrode array (20) when the working film (10) of the cartridge (1) is placed on said electrode array (20). The cartridge (1) comprises a body (2,2′,2″) with a number of wells (5) configured to hold therein reagents (6) or samples (6′); a flexibly deformable top structure (7) impermeable to liquids and configured to seal a top side of the wells (5); a piercable bottom structure (8) impermeable to liquids and configured to seal a bottom side of the wells (5); a working film (10) located below a lower surface (4) of the body (2,2′,2″), the working film (10) being impermeable to liquids and comprising a hydrophobic upper surface (11); a peripheral spacer (9,9′,9″) located below the lower surface (4) of the body (2,2′,2″) and connecting the working film (10) to the body (2,2′,2″); a gap (12) between the lower surface (4) of the body (2,2′,2″) and the hydrophobic upper surface (11) of the working film (10), the gap (12) being defined by the peripheral spacer (9,9′,9″); and a number of piercing elements (13) located below piercable bottom structures (8) and configured to pierce the piercable bottom structures (8) for releasing reagents or samples (6,6′) from the wells (5) into the gap (12). Also disclosed is a system (40) with an electrode array (20) onto which the cartridge (1) can be placed.
    • 当药筒(1)的工作薄膜(10)被放置在所述电极阵列(20)上时,墨盒(1)包括用于用电极阵列(20)操纵液滴样品的工作薄膜(10)。 墨盒(1)包括具有多个孔(5)的主体(2,2',2“),所述孔被配置为容纳其中的试剂(6)或样品(6'); 柔性可变形顶部结构(7),其不透液体并且构造成密封所述孔(5)的顶侧; 不透液体并且被构造成密封井(5)的底侧的可穿透底部结构(8); 位于主体(2,2',2“)的下表面(4)下方的工作薄膜(10),工作薄膜(10)不透液体并包括疏水性上表面(11); 位于主体(2,2',2“)的下表面(4)下方的外围间隔件(9,9',9”),并将工作膜(10)连接到主体(2,2' ,2“); 主体(2,2',2“)的下表面(4)与工作膜(10)的疏水性上表面(11)之间的间隙(12),间隙(12)由 外围间隔物(9,9',9“); 以及位于穿孔底部结构(8)下面的多个穿孔元件(13),并被构造成刺穿可穿透底部结构(8),用于将试剂或样品(6,6')从孔(5)释放到间隙(12) )。 还公开了一种具有电极阵列(20)的系统(40),其上可以放置盒(1)。