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    • 5. 发明授权
    • Seed-preferred regulatory elements
    • 种子优先的监管要素
    • US07211712B2
    • 2007-05-01
    • US11284201
    • 2005-11-21
    • Shane E. AbbittRudolf Jung
    • Shane E. AbbittRudolf Jung
    • C12P7/62C12P11/00C07H21/04A01H1/00
    • C12N15/8234
    • The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a seed-preferred promoter and terminator isolated from the maize legumin 1 coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
    • 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是从玉米豆科1编码区分离的种子优选启动子和终止子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的一个或多个调控序列的异源核苷酸序列,并从转化的植物细胞再生稳定转化的植物。
    • 8. 发明授权
    • Methods of increasing polypeptide accumulation in plants
    • 增加植物多肽积累的方法
    • US07557263B2
    • 2009-07-07
    • US11971708
    • 2008-01-09
    • Darren B. GruisRudolf Jung
    • Darren B. GruisRudolf Jung
    • C12N15/82C12N15/87C01H21/20A01H5/00
    • C07K16/00C07K2317/13C07K2317/622C12N9/50C12N15/8251C12N15/8257C12N15/8258
    • The present invention provides methods and compositions directed to the production of polypeptides in plant protein storage tissue. Methods of the invention include methods for producing polypeptides in protein storage tissues by decreasing the level of protease activity in the plant protein storage tissue. Compositions of the invention include plants that are genetically modified such that the activity of one or more proteases is reduced or eliminated, transformed seeds obtained from these genetically modified plants, and sequences encoding a novel member of the VPE cysteine protease family found in seed, ε-VPE. These methods and compositions find utility in altering the nutritional or other qualities of seed, and in producing useful proteins including pharmacological or industrial proteins.
    • 本发明提供了用于在植物蛋白质储存组织中产生多肽的方法和组合物。 本发明的方法包括通过降低植物蛋白质储存组织中蛋白酶活性的水平来在蛋白质储存组织中产生多肽的方法。 本发明的组合物包括被遗传修饰的植物,使得一种或多种蛋白酶的活性被降低或消除,从这些遗传修饰的植物获得的转化的种子,以及编码在种子中的VPE半胱氨酸蛋白酶家族的新成员的序列, -VPE。 这些方法和组合物可用于改变种子的营养或其他品质,以及生产有用的蛋白质,包括药理学或工业蛋白质。