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1. 发明授权

US5981238A Process for producing optically active aminopolycarboxylic acid 失效
标题翻译：光学活性氨基多羧酸的制备方法
公开(公告)号：US5981238A
公开(公告)日：1999-11-09
申请号：US841663
申请日：1997-04-30
申请人： Makoto Kaneko , Yoshihiro Hashimoto , Takakazu Endo , Mami Kato , Wataru Mizunashi
发明人： Makoto Kaneko , Yoshihiro Hashimoto , Takakazu Endo , Mami Kato , Wataru Mizunashi
IPC分类号： C12P13/04 , C12P13/20
CPC分类号： C12P13/04
摘要： A process for producing an optically active aminopolycarboxylic acid, such as S,S-ethylenediamine-N,N'-disuccinic acid, from a mixture of a diamine, such as ethylenediamine, with fumaric acid using a microorganism having a lyase activity, wherein at least one metal ion selected from the group consisting of an alkaline earth metal, iron, zinc, copper, nickel, aluminum, titanium and manganese is added to the reaction system. According to this process, aminopolycarboxylic acids, such as S,S-ethylenediamine-N,N'-disuccinic acid, or metal complexes thereof, can be appropriately and efficiently produced while improving the reaction yield.
摘要翻译：使用具有裂解酶活性的微生物从二胺（例如乙二胺）与富马酸的混合物制备光学活性氨基多羧酸如S，S-乙二胺-N，N'-二琥珀酸的方法，其中在将至少一种选自碱土金属，铁，锌，铜，镍，铝，钛和锰的金属离子加入到反应体系中。根据该方法，可以在提高反应收率的同时，适当且有效地制造氨基多元羧酸，如S，S-乙二胺-N，N'-二琥珀酸或其金属络合物。

2. 发明授权

US6136573A Process for producing alkali metal [S,S]-ethylenediamine-n,n'-disuccinates 有权
标题翻译：制备碱金属[S，S] - 乙二胺-N，N'-二琥珀酸盐的方法
公开(公告)号：US6136573A
公开(公告)日：2000-10-24
申请号：US446195
申请日：1999-12-17
申请人： Makoto Kaneko , Takakazu Endo , Toshihiko Fukuda , Mami Kato
发明人： Makoto Kaneko , Takakazu Endo , Toshihiko Fukuda , Mami Kato
IPC分类号： C12P13/00
CPC分类号： C12P13/001
摘要： A process for producing an [S,S]-ethylenediamine-N,N'-disuccinic acid alkali metal salt which comprises reacting fumaric acid with ethylenediamine in an aqueous medium in the presence of an ion of at least one metal selected from the group consisting of alkaline earth metals and transition metals by the action of ethylenediamine-disuccinic acid ethylenediamine lyase of microorganism origin, adding an alkali hydroxide to a reaction product mixture thereby to separate and recover the metal ion as an insoluble precipitate, and obtaining an [S,S]-ethylenediamine-N,N'-disuccinic acid alkali metal salt.
摘要翻译： PCT No.PCT / JP99 / 02003 Sec。 371 1999年12月17日第 102（e）1999年12月17日PCT PCT 1999年4月15日PCT公布。 WO99 / 54492 PCT出版物日期1999年10月28日制备[S，S] - 乙二胺-N，N'-二琥珀酸碱金属盐的方法，其包括在水性介质中在至少一种金属离子存在下使富马酸与乙二胺反应选自微生物来源的乙二胺 - 二琥珀酸乙二胺裂解酶的作用，由碱土金属和过渡金属组成的组中选择，向反应产物混合物中加入碱金属氢氧化物，从而将金属离子分离并回收为不溶性沉淀，得到 [S，S] - 乙二胺-N，N'-二琥珀酸碱金属盐。

3. 发明授权

US6103508A Method for removing fumarase activity, microorganisms obtainable by the method, and production of optically active aminopolycarboxylic acids using the microorganisms 有权
标题翻译：消除富马酸酶活性的方法，通过该方法获得的微生物和使用微生物制备光学活性氨基多羧酸的方法
公开(公告)号：US6103508A
公开(公告)日：2000-08-15
申请号：US179966
申请日：1998-10-28
申请人： Mami Kato , Makoto Kaneko , Takakazu Endo
发明人： Mami Kato , Makoto Kaneko , Takakazu Endo
IPC分类号： C12N1/38 , C12N9/00 , C12N9/99 , C12P13/04 , C12P13/00 , C12N9/04
CPC分类号： C12N9/93 , C12N1/38 , C12N9/99 , C12P13/04 , Y10S435/80 , Y10S435/82 , Y10S435/822 , Y10S435/83 , Y10S435/848 , Y10S435/874
摘要： This invention relates to a method for removing fumarase activity from a microorganism or processed product thereof having ethylenediamine-N,N'-disuccinic acid ethylenediamine lyase activity, which includes treating the microorganism or processed product thereof with an aqueous alkaline solution at a pH of 8.0 to 10.5 in the presence of at least one salt with a concentration of 5 mM to 1000 mM. The salt is preferably selected from the group consisting of sodium, potassium, ammonium and C.sub.2-6 alkanediamine salts of boric acid, phosphoric acid, hydrochloric acid, sulfuric acid, acetic acid, oxalic acid, fumaric acid, maleic acid and ethylenediamine-N,N'-disuccinic acid, and mixtures thereof. This invention also relates to a microorganism or processed product thereof having reduced fumarase activity obtainable by the above described method, and to a method for producing an optically active aminopolycarboxylic acid from fumaric acid and a compound having amino group in the presence of the microorganim or processed product thereof having reduced fumarase activity as a catalyst.
摘要翻译：本发明涉及一种从具有乙二胺-N，N'-二琥珀酸乙二胺裂解酶活性的微生物或其加工产物中除去富马酸酯活性的方法，其包括用pH为8.0的碱性水溶液处理微生物或其加工产物在存在至少一种浓度为5mM至1000mM的盐的情况下为10.5。该盐优选选自硼酸，磷酸，盐酸，硫酸，乙酸，草酸，富马酸，马来酸和乙二胺-N的钠，钾，铵和C 2-6烷二胺盐， N'-二琥珀酸，及其混合物。本发明还涉及通过上述方法获得的具有降低的延胡索酸酶活性的微生物或其加工产物，以及在微生物存在下由富马酸和具有氨基的化合物制备光学活性氨基多羧酸的方法或加工其产物具有降低的富马酸酯活性作为催化剂。

4. 发明授权

US06261798B1 Methods for producing [S,S]-ethylenediamine-N,N′-disuccinate 有权
标题翻译： [S，S] - 乙二胺-N，N'-二琥珀酸盐的制备方法
公开(公告)号：US06261798B1
公开(公告)日：2001-07-17
申请号：US09582171
申请日：2000-06-22
申请人： Osamu Kato , Makoto Kaneko , Takakazu Endo
发明人： Osamu Kato , Makoto Kaneko , Takakazu Endo
IPC分类号： C12P1302
CPC分类号： C12P13/02
摘要： The present invention relates to a method of producing [S,S]-ethylenediamine-N,N′-disuccinate wherein a microorganism having malate isomerase activity or matter processed therefrom and a microorganism having ethylenediamine-N,N′-disuccinate ethylenediamine lyase activity or matter processed therefrom are allowed to act on a substrate solution containing maleic acid, maleic anhydride, or a maleic acid salt, and ethylenediamine, in the presence of at least one metal ion selected from the group consisting of alkaline earth metals, iron, zinc, copper, nickel, aluminum, titanium and manganese. The present invention enables to accumulate [S,S]-ethylenediamine-N,N′-disuccinate in a higher yield and at a high concentration within a reaction system using maleic acid as a raw material.
摘要翻译：本发明涉及一种生产[S，S] - 乙二胺-N，N'-二琥珀酸盐的方法，其中具有苹果酸异构酶活性的微生物或由其处理的物质和具有乙二胺-N，N'-二琥珀酸乙二胺裂解酶活性的微生物或在其中处理的物质可以在至少一种选自碱土金属，铁，锌，锌等的金属离子的存在下作用于含有马来酸，马来酸酐或马来酸盐和乙二胺的底物溶液，铜，镍，铝，钛和锰。本发明能够以马来酸为原料，在反应体系内以更高的收率和高浓度积累[S，S] - 乙二胺-N，N'-二琥珀酸盐。

5. 发明授权

US5705382A Method for preserving nitrile hydratase or nitrilase activity of microbial cells with inorganic salts 失效
标题翻译：用无机盐保存微生物细胞的腈水合酶或腈水解酶活性的方法
公开(公告)号：US5705382A
公开(公告)日：1998-01-06
申请号：US542291
申请日：1995-10-12
申请人： Takakazu Endo , Toshiaki Doi , Koji Tamura , Yuji Hirata , Kouzo Murao
发明人： Takakazu Endo , Toshiaki Doi , Koji Tamura , Yuji Hirata , Kouzo Murao
IPC分类号： C12N11/00 , C12N1/04 , C12N11/14 , C12R1/01 , C12N1/12 , C12N9/96 , C12N11/04
CPC分类号： C12N1/04 , C12N11/14
摘要： Cells having enzyme activity, and the enzyme activity thereof, are preserved for a prolonged period of time, as a suspension of microbial cells or as a suspension of immobilized cells in particles, in an aqueous medium that is a neutral or weakly basic aqueous solution of inorganic salts, having a molarity ranging from 100 mM to the saturation concentration of the inorganic salts. Preferably, the microbial cells are cells containing the enzyme, nitrile hydratase or nitrilase, such as Gordona terrae or Rhodococcus rhodochrous, and the inorganic salts are phosphates, borates, sulfates, sulfites or hydrochlorides. The present invention provides an industrially useful method for preserving a large quantity of cells or immobilized cells in particles having nitrile hydratase or nitrilase enzyme activity for a prolonged period of time (e.g., 300 days) without cell lysis or enzyme deterioration even at room temperature. The present invention also renders possible a sharp reduction in labor and cooling cost, which are necessary in the conventional preservation process.
摘要翻译：具有酶活性的细胞及其酶活性在水性培养基中作为微生物细胞的悬浮液或作为固定化细胞的悬浮液在水性培养基中保持较长时间，其为中性或弱碱性水溶液无机盐，其摩尔浓度范围为100mM至无机盐的饱和浓度。优选地，微生物细胞是含有酶，腈水合酶或腈水解酶的细胞，例如戈多纳土壤或玫瑰色红玫瑰色，无机盐是磷酸盐，硼酸盐，硫酸盐，亚硫酸盐或盐酸盐。本发明提供一种工业上有用的方法，即使在室温下，即使在室温下，也不会在细胞裂解或酶劣化的情况下长时间（例如，300天），在具有腈水合酶或腈水解酶活性的颗粒中保存大量细胞或固定化细胞。本发明还使得在常规保存过程中必需的劳动和冷却成本急剧下降。

6. 发明授权

US5223416A Process for producing R(-)-mandelic acid and derivatives thereof 失效
标题翻译：制备R（ - ） - 马来酸及其衍生物的方法
公开(公告)号：US5223416A
公开(公告)日：1993-06-29
申请号：US677175
申请日：1991-03-29
申请人： Takakazu Endo , Koji Tamura
发明人： Takakazu Endo , Koji Tamura
IPC分类号： C12P7/42 , C12P41/00
CPC分类号： C12P41/006 , C12P7/42 , Y10S435/822 , Y10S435/829 , Y10S435/839 , Y10S435/84 , Y10S435/872 , Y10S435/874
摘要： A process for the predominantly producing R(-)-mandelic acid or a derivative thereof which comprises subjecting (i) R,S-mandelonitrile or a derivative thereof, or (ii) a mixture of prussic acid and benzaldehyde or a derivative of benzaldehyde to the action of a microorganism selected from the group consisting of the genus Aureobacterium, Pseudomonas, Caseobacter, Alcaligenes, Acinetobacter, Brevibacterium, Nocardia, and Bacillus or treated cells thereof, which the microorganism is capable of stereospecifically hydrolyzing a nitrile group of the R,S-mandelonitrile or a derivative thereof, in a neutral or basic aqueous reaction system to produce the R(-)-mandelic acid.

7. 发明授权

US06495716B2 [S,S]-ethylenediamine-N,N′-disuccinic acid crystal with high bulk density and process for obtaining the same 有权
标题翻译：具有高堆积密度的[S，S] - 乙二胺-N，N'-二琥珀酸晶体及其制备方法
公开(公告)号：US06495716B2
公开(公告)日：2002-12-17
申请号：US10083536
申请日：2002-02-27
申请人： Hiroyasu Banba , Shigeho Tanaka , Kiyonobu Niwa , Takakazu Endo
发明人： Hiroyasu Banba , Shigeho Tanaka , Kiyonobu Niwa , Takakazu Endo
IPC分类号： C07C22900
CPC分类号： C07C229/24 , C07C227/42
摘要： Crystals of [S,S]-ethylenediamine-N,N′-disuccinic acid which have a bulk density of 0.45 to 1.2 g/cm3, and a process for obtaining these crystals comprising the steps of adjusting an aqueous solution of an [S,S]-ethylenediamine-N,N′-disuccinic acid metal salt to a temperature of 40 to 80° C. and a pH of 1.9 to 4.5, and cooling the solution to a temperature below 40° C. over a period of 0.2 to 10 hours while supplying a mineral acid so as to maintain the pH at 1.9 to 4.5 to precipitate [S,S]-ethylenediamine-N,N′-disuccinic acid in a high yield.
摘要翻译：具有堆积密度为0.45〜1.2g / cm 3的[S，S] - 乙二胺-N，N'-二琥珀酸的晶体以及获得这些结晶的方法，包括以下步骤：将[S， S] - 乙二胺-N，N'-二琥珀酸金属盐，温度为40〜80℃，pH为1.9〜4.5，在0.2〜 10小时，同时供给无机酸，以保持pH为1.9〜4.5，以高收率沉淀出[S，S] - 乙二胺-N，N'-二琥珀酸。

8. 发明授权

US5238827A Process for preparing glycine from glycinonitrile 失效
标题翻译：从甘氨酸制备甘氨酸的方法
公开(公告)号：US5238827A
公开(公告)日：1993-08-24
申请号：US677176
申请日：1991-03-29
申请人： Hitoshi Shimizu , Chiharu Fujita , Takakazu Endo , Ichiro Watanabe
发明人： Hitoshi Shimizu , Chiharu Fujita , Takakazu Endo , Ichiro Watanabe
IPC分类号： C12P13/04 , C12R1/01 , C12R1/05 , C12R1/06 , C12R1/15 , C12R1/38 , C12R1/545
CPC分类号： C12P13/04
摘要： A process for preparing glycine from glycinonitrile by reacting glycinonitrile with a hydrolase produced by a microorganism, wherein the microorganism is a member of a genus selected from the group consisting of Rhodococcus, Arthrobacter, Caseobacter, Pseudomonas, Enterobacter, Acinetobacter, Alcaliqenes, and Streptomyces. The process involves no by-production, and the microorganism has high hydrolizing activity to convert glycinonitrile to glycine.

9. 发明授权

US06414189B1 Crystals of [S,S]-ethylenediamine-N,N′-disuccinic acid with high bulk density and method of obtaining the same 有权
标题翻译：具有高堆积密度的[S，S] - 乙二胺-N，N'-二琥珀酸的晶体和获得相同的方法
公开(公告)号：US06414189B1
公开(公告)日：2002-07-02
申请号：US09530964
申请日：2000-05-18
申请人： Hiroyasu Banba , Shigeho Tanaka , Kiyonobu Niwa , Takakazu Endo
发明人： Hiroyasu Banba , Shigeho Tanaka , Kiyonobu Niwa , Takakazu Endo
IPC分类号： C07C22900
CPC分类号： C07C229/24 , C07C227/42
摘要： Crystals of [S,S]-ethylenediamine-N,N′-disuccinic acid which have a bulk density of 0.45 to 1.2 g/cm3, and a process for obtaining these crystals comprising the steps of adjusting an aqueous solution of an [S,S]-ethylenediamine-N,N′-disuccinic acid metal salt to a temperature of 40 to 80° C. and a pH of 1.9 to 4.5, and cooling the solution to a temperature below 40° C. over a period of 0.2 to 10 hours while supplying a mineral acid so as to maintain the pH at 1.9 to 4.5 to precipitate [S,S]-ethylenediamine-N,N′-disuccinic acid in a high yield.
摘要翻译：具有堆积密度为0.45〜1.2g / cm 3的[S，S] - 乙二胺-N，N'-二琥珀酸的晶体以及获得这些结晶的方法，包括以下步骤：将[S， S] - 乙二胺-N，N'-二琥珀酸金属盐，温度为40〜80℃，pH为1.9〜4.5，在0.2〜 10小时，同时供给无机酸，以保持pH为1.9〜4.5，以高收率沉淀出[S，S] - 乙二胺-N，N'-二琥珀酸。

10. 发明授权

US5580765A Process for producing optically active a-hydroxycarboxylic acid having phenyl group using gordona terrae 失效
标题翻译：使用戈多纳土生产具有苯基的光学活性α-羟基羧酸的方法
公开(公告)号：US5580765A
公开(公告)日：1996-12-03
申请号：US191164
申请日：1994-02-03
申请人： Yoshihiro Hashimoto , Takakazu Endo , Koji Tamura , Yuji Hirata
发明人： Yoshihiro Hashimoto , Takakazu Endo , Koji Tamura , Yuji Hirata
IPC分类号： C12P7/42 , C12P41/00 , C12R1/01
CPC分类号： C12P41/006 , C12P7/42 , Y10S435/822
摘要： A biological process for predominantly producing an optically active .alpha.-hydroxycarboxylic acid having a phenyl group directly from a racemic .alpha.-hydroxynitrile or a mixture of an aldehyde corresponding to the nitrile and prussic acid as a substrate is disclosed, comprising reacting a microorganism belonging to the genus Gordona with the substrate in a neutral to basic aqueous medium. A desired optically active .alpha.-hydroxycarboxylic acid having a phenyl group can be obtained quantitatively at a high optical purity.
摘要翻译：公开了一种用于主要生产具有直接来自外消旋α-羟基腈的苯基的光学活性α-羟基羧酸或对应于腈和氢醌作为底物的醛的混合物的生物方法，其包括将属于 Gordona属与中性至碱性水性介质中的底物。可以以高光学纯度定量获得具有苯基的所需光学活性α-羟基羧酸。

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