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    • 4. 发明授权
    • Methods for producing [S,S]-ethylenediamine-N,N′-disuccinate
    • [S,S] - 乙二胺-N,N'-二琥珀酸盐的制备方法
    • US06261798B1
    • 2001-07-17
    • US09582171
    • 2000-06-22
    • Osamu KatoMakoto KanekoTakakazu Endo
    • Osamu KatoMakoto KanekoTakakazu Endo
    • C12P1302
    • C12P13/02
    • The present invention relates to a method of producing [S,S]-ethylenediamine-N,N′-disuccinate wherein a microorganism having malate isomerase activity or matter processed therefrom and a microorganism having ethylenediamine-N,N′-disuccinate ethylenediamine lyase activity or matter processed therefrom are allowed to act on a substrate solution containing maleic acid, maleic anhydride, or a maleic acid salt, and ethylenediamine, in the presence of at least one metal ion selected from the group consisting of alkaline earth metals, iron, zinc, copper, nickel, aluminum, titanium and manganese. The present invention enables to accumulate [S,S]-ethylenediamine-N,N′-disuccinate in a higher yield and at a high concentration within a reaction system using maleic acid as a raw material.
    • 本发明涉及一种生产[S,S] - 乙二胺-N,N'-二琥珀酸盐的方法,其中具有苹果酸异构酶活性的微生物或由其处理的物质和具有乙二胺-N,N'-二琥珀酸乙二胺裂解酶活性的微生物或 在其中处理的物质可以在至少一种选自碱土金属,铁,锌,锌等的金属离子的存在下作用于含有马来酸,马来酸酐或马来酸盐和乙二胺的底物溶液, 铜,镍,铝,钛和锰。 本发明能够以马来酸为原料,在反应体系内以更高的收率和高浓度积累[S,S] - 乙二胺-N,N'-二琥珀酸盐。
    • 5. 发明授权
    • Method for preserving nitrile hydratase or nitrilase activity of
microbial cells with inorganic salts
    • 用无机盐保存微生物细胞的腈水合酶或腈水解酶活性的方法
    • US5705382A
    • 1998-01-06
    • US542291
    • 1995-10-12
    • Takakazu EndoToshiaki DoiKoji TamuraYuji HirataKouzo Murao
    • Takakazu EndoToshiaki DoiKoji TamuraYuji HirataKouzo Murao
    • C12N11/00C12N1/04C12N11/14C12R1/01C12N1/12C12N9/96C12N11/04
    • C12N1/04C12N11/14
    • Cells having enzyme activity, and the enzyme activity thereof, are preserved for a prolonged period of time, as a suspension of microbial cells or as a suspension of immobilized cells in particles, in an aqueous medium that is a neutral or weakly basic aqueous solution of inorganic salts, having a molarity ranging from 100 mM to the saturation concentration of the inorganic salts. Preferably, the microbial cells are cells containing the enzyme, nitrile hydratase or nitrilase, such as Gordona terrae or Rhodococcus rhodochrous, and the inorganic salts are phosphates, borates, sulfates, sulfites or hydrochlorides. The present invention provides an industrially useful method for preserving a large quantity of cells or immobilized cells in particles having nitrile hydratase or nitrilase enzyme activity for a prolonged period of time (e.g., 300 days) without cell lysis or enzyme deterioration even at room temperature. The present invention also renders possible a sharp reduction in labor and cooling cost, which are necessary in the conventional preservation process.
    • 具有酶活性的细胞及其酶活性在水性培养基中作为微生物细胞的悬浮液或作为固定化细胞的悬浮液在水性培养基中保持较长时间,其为中性或弱碱性水溶液 无机盐,其摩尔浓度范围为100mM至无机盐的饱和浓度。 优选地,微生物细胞是含有酶,腈水合酶或腈水解酶的细胞,例如戈多纳土壤或玫瑰色红玫瑰色,无机盐是磷酸盐,硼酸盐,硫酸盐,亚硫酸盐或盐酸盐。 本发明提供一种工业上有用的方法,即使在室温下,即使在室温下,也不会在细胞裂解或酶劣化的情况下长时间(例如,300天),在具有腈水合酶或腈水解酶活性的颗粒中保存大量细胞或固定化细胞。 本发明还使得在常规保存过程中必需的劳动和冷却成本急剧下降。