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1. 发明授权

US08828678B2 Self-immolative probes for enzyme activity detection 有权
标题翻译：用于酶活性检测的自发性探针
公开(公告)号：US08828678B2
公开(公告)日：2014-09-09
申请号：US12927497
申请日：2010-11-16
申请人： Maciej Szczepanik , Irina V. Lebedeva , Yuejun Xiang , Praveen Pande , Wayne Forrest Patton
发明人： Maciej Szczepanik , Irina V. Lebedeva , Yuejun Xiang , Praveen Pande , Wayne Forrest Patton
IPC分类号： C12Q1/26 , C07D311/02 , C07D305/14 , C07D413/00 , G01N21/00
CPC分类号： C12Q1/26 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10 , C09K11/06 , C09K2211/1022 , C09K2211/1088 , C12Q1/37 , C12Q1/44 , C12Q1/485 , G01N2333/902 , G01N2500/00 , Y10T436/18
摘要： A compound comprising the structure: (SIG)-(SI-MOD)m. is provided in which SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample comprises an activator such as a nitroreductase, and methods of determining whether a mammalian cell is hypoxic using a compound of the formula (SIG)-(SI-MOD)m.
摘要翻译：包含以下结构的化合物：（SIG） - （SI-MOD）m。其中SIG是信号分子，SI是与SIG结合的自消除结构，使得SIG相对于没有SI的SIG的信号具有减少的信号，MOD是与SI结合的部分，其经受活化剂，m是1至约10的整数。当通过活化剂修饰MOD时，SI是不稳定的并且从SIG自分离，使得SIG产生增加的信号。还提供了确定样品是否包含诸如硝酸还原酶的活化剂的方法以及使用式（SIG） - （SI-MOD）m的化合物来确定哺乳动物细胞是否缺氧的方法。

2. 发明申请

US20120122136A1 Self-immolative probes for enzyme activity detection 有权
标题翻译：用于酶活性检测的自发性探针
公开(公告)号：US20120122136A1
公开(公告)日：2012-05-17
申请号：US12927497
申请日：2010-11-16
申请人： Maciej Szczepanik , Irina V. Lebedeva , Yuejun Xiang , Praveen Pande , Wayne Forrest Patton
发明人： Maciej Szczepanik , Irina V. Lebedeva , Yuejun Xiang , Praveen Pande , Wayne Forrest Patton
IPC分类号： C12Q1/26 , C07D491/22 , G01N21/75 , C07D493/10 , C07D413/14 , C07D237/32 , C07D311/08
CPC分类号： C12Q1/26 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10 , C09K11/06 , C09K2211/1022 , C09K2211/1088 , C12Q1/37 , C12Q1/44 , C12Q1/485 , G01N2333/902 , G01N2500/00 , Y10T436/18
摘要： Provided is a compound comprising the structure: (SIG)-(SI-MOD)m. In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.
摘要翻译：提供了包含以下结构的化合物：（SIG） - （SI-MOD）m。在该化合物中，SIG是信号分子，SI是与SIG结合的自消除结构，使得SIG相对于没有SI的SIG的信号具有降低的信号，MOD是与SI结合的部分，其经受活化剂，m为1至约10的整数。用该化合物，当MOD被活化剂修饰时，SI是不稳定的并且从SIG自分离，使得SIG产生增加的信号。还提供了使用上述化合物来确定样品是否包含活化剂的方法。另外提供了使用上述硝基还原酶是活化剂的化合物来确定细胞是否包含硝基还原酶的方法。还提供了使用上述硝基还原酶是活化剂的化合物来确定哺乳动物细胞是否缺氧的方法。还提供了使用上述硝基还原酶为活化剂的化合物检测包含硝基还原酶的微生物的方法。还提供了使用上述硝酸还原酶是活化剂的化合物来鉴定样品中的硝基还原酶的方法。

3. 发明申请

US20100081159A1 Profiling reactive oxygen, nitrogen and halogen species 审中-公开
标题翻译：分析活性氧，氮和卤素
公开(公告)号：US20100081159A1
公开(公告)日：2010-04-01
申请号：US12286103
申请日：2008-09-26
申请人： Irina V. Lebedeva , Wayne Forrest Patton
发明人： Irina V. Lebedeva , Wayne Forrest Patton
IPC分类号： C12Q1/02
CPC分类号： G01N33/582 , C12Q1/02 , G01N15/14 , G01N33/5005
摘要： This invention provides methods, kits and systems which permit simultaneous profiling of multiple reactive oxygen species (ROS), reactive nitrogen species (RNS) and/or reactive halogen species (RHS) including reactive chlorine species (RCS) and/or reactive bromine species (RBS) through multiplexed fluorescence detection of three or more indicator probes in live cells or subcellular organelles.
摘要翻译：本发明提供方法，试剂盒和系统，其允许包括反应性氯物质（RCS）和/或反应性溴物质（RCS）和/或反应性溴物质的多种活性氧（ROS），活性氮物质（RNS）和/或反应性卤素物质（RHS） RBS）通过活细胞或亚细胞器中的三个或更多个指示物探针的多重荧光检测。

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